A rapid and sensitive lateral flow immunoassay (LFIA) for the detection of gluten in foods
•Monoclonal antibodies for sensitive and selective gluten detection.•Rapid gluten detection (
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| Veröffentlicht in: | Food chemistry 2021-09, Vol.355, p.129514-129514, Article 129514 |
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| container_end_page | 129514 |
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| container_issue | |
| container_start_page | 129514 |
| container_title | Food chemistry |
| container_volume | 355 |
| creator | Hnasko, Robert M. Jackson, Eric S. Lin, Alice V. Haff, Ronald P. McGarvey, Jeffery A. |
| description | •Monoclonal antibodies for sensitive and selective gluten detection.•Rapid gluten detection ( |
| doi_str_mv | 10.1016/j.foodchem.2021.129514 |
| format | Article |
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The gluten protein found in a variety of cereal grains is a food allergen that can elicit a spectrum of immuno-inflammatory responses in people. Consumer awareness has prompted changes in food labeling requirements, expanded gluten-free food product availability and increased demand for effective gluten testing methodologies. To meet the challenges associated with gluten testing from diverse and complex foods we developed a lateral flow immunoassay (LFIA) using a pair of novel gliadin monoclonal antibodies (MAbs). Using a visual gold reporter, we show sensitive gluten detection (150 ng/mL) from complex food substrates using a fast (<5 min) and easy testing methodology. 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The gluten protein found in a variety of cereal grains is a food allergen that can elicit a spectrum of immuno-inflammatory responses in people. Consumer awareness has prompted changes in food labeling requirements, expanded gluten-free food product availability and increased demand for effective gluten testing methodologies. To meet the challenges associated with gluten testing from diverse and complex foods we developed a lateral flow immunoassay (LFIA) using a pair of novel gliadin monoclonal antibodies (MAbs). Using a visual gold reporter, we show sensitive gluten detection (150 ng/mL) from complex food substrates using a fast (<5 min) and easy testing methodology. In this report we characterize the binding properties of a cohort of newly generated gliadin monoclonal antibodies suitable for gluten detection using multiple assay formats and introduce a novel plug-n-play test strip platform with integrated test components in a single-use format.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>33774225</pmid><doi>10.1016/j.foodchem.2021.129514</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0308-8146 |
| ispartof | Food chemistry, 2021-09, Vol.355, p.129514-129514, Article 129514 |
| issn | 0308-8146 1873-7072 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - immunology Biosensor Food allergen Food Analysis - methods Food Labeling Gliadin Gliadin - immunology Gluten Glutens - analysis Glutens - immunology Gold - chemistry Humans Immunoassay - methods Lateral Flow Immunoassay (LFIA) Limit of Detection Monoclonal antibodies Time Factors |
| title | A rapid and sensitive lateral flow immunoassay (LFIA) for the detection of gluten in foods |
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