TRIF-mediated antiviral signaling is differentially regulated by TRAF2 and TRAF6 in black carp
TRIF is an antiviral adaptor downstream of Toll-like receptors, the roles of teleost TRIF and their regulation remain largely unknown. In this study, a TRIF homologue (bcTRIF) of black carp (Mylopharyngodon piceus) has been cloned, and the transcription of bcTRIF in vivo and ex vivo increased in res...
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| Veröffentlicht in: | Developmental and comparative immunology 2021-08, Vol.121, p.104073-104073, Article 104073 |
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| container_title | Developmental and comparative immunology |
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| creator | Peng, Fei Jin, Saisai Chen, Zhaoyuan Chang, Haiyan Xiao, Jun Li, Jianzhong Zou, Jun Feng, Hao |
| description | TRIF is an antiviral adaptor downstream of Toll-like receptors, the roles of teleost TRIF and their regulation remain largely unknown. In this study, a TRIF homologue (bcTRIF) of black carp (Mylopharyngodon piceus) has been cloned, and the transcription of bcTRIF in vivo and ex vivo increased in response to different stimuli. Overexpressed bcTRIF induced the transcription of interferon promoter in the EPC cells and enhanced protection of cells against infection of spring viremia of carp virus (SVCV). The previous study has identified that black carp TRAF2 (bcTRAF2) and TRAF6 (bcTRAF6) functioned positively in RIG-I/MAVS signaling. When co-expressed with bcTRAF2, bcTRIF-induced the transcription of interferon promoter in EPC cells was decreased, and the antiviral activity of bcTRIF was dampened accordingly. On the contrary, co-expressed bcTRAF6 enhanced both bcTRIF-mediated interferon promoter transcription and antiviral activity. The subsequent co-immunoprecipitation identified the interaction between bcTRAF2/6 and bcTRIF. Thus, bcTRIF-mediated antiviral signaling is up-regulated by bcTRAF6 and down-regulated by bcTRAF2.
•bcTRIF interacts with both bcTRAF2 and bcTRAF6.•bcTRIF-mediated antiviral signaling is positively regulated by bcTRAF6.•bcTRIF-mediated antiviral signaling is negatively regulated by bcTRAF2. |
| doi_str_mv | 10.1016/j.dci.2021.104073 |
| format | Article |
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•bcTRIF interacts with both bcTRAF2 and bcTRAF6.•bcTRIF-mediated antiviral signaling is positively regulated by bcTRAF6.•bcTRIF-mediated antiviral signaling is negatively regulated by bcTRAF2.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2021.104073</identifier><identifier>PMID: 33766587</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Adaptor Proteins, Vesicular Transport - genetics ; Adaptor Proteins, Vesicular Transport - metabolism ; Animals ; Antiviral activity ; Black carp ; Carp ; Carps - genetics ; Carps - immunology ; Carps - virology ; Cloning, Molecular ; Down-Regulation - immunology ; Fish Diseases - immunology ; Fish Diseases - virology ; Fish Proteins - genetics ; Fish Proteins - metabolism ; Homology ; Immunoprecipitation ; Interferon ; Receptors, Immunologic - genetics ; Receptors, Immunologic - metabolism ; Signaling ; TNF Receptor-Associated Factor 2 - metabolism ; TNF Receptor-Associated Factor 6 - metabolism ; Toll-like receptors ; TRAF2 ; TRAF2 protein ; TRAF6 ; TRAF6 protein ; Transcription ; TRIF ; Up-Regulation - immunology ; Viremia ; Viruses</subject><ispartof>Developmental and comparative immunology, 2021-08, Vol.121, p.104073-104073, Article 104073</ispartof><rights>2021 Elsevier Ltd</rights><rights>Copyright © 2021 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Aug 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-aedcf8a647f53d5c21d62f306125b6283c8000f36038f61b436d6b8a623204c33</citedby><cites>FETCH-LOGICAL-c381t-aedcf8a647f53d5c21d62f306125b6283c8000f36038f61b436d6b8a623204c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2021.104073$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33766587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Fei</creatorcontrib><creatorcontrib>Jin, Saisai</creatorcontrib><creatorcontrib>Chen, Zhaoyuan</creatorcontrib><creatorcontrib>Chang, Haiyan</creatorcontrib><creatorcontrib>Xiao, Jun</creatorcontrib><creatorcontrib>Li, Jianzhong</creatorcontrib><creatorcontrib>Zou, Jun</creatorcontrib><creatorcontrib>Feng, Hao</creatorcontrib><title>TRIF-mediated antiviral signaling is differentially regulated by TRAF2 and TRAF6 in black carp</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>TRIF is an antiviral adaptor downstream of Toll-like receptors, the roles of teleost TRIF and their regulation remain largely unknown. In this study, a TRIF homologue (bcTRIF) of black carp (Mylopharyngodon piceus) has been cloned, and the transcription of bcTRIF in vivo and ex vivo increased in response to different stimuli. Overexpressed bcTRIF induced the transcription of interferon promoter in the EPC cells and enhanced protection of cells against infection of spring viremia of carp virus (SVCV). The previous study has identified that black carp TRAF2 (bcTRAF2) and TRAF6 (bcTRAF6) functioned positively in RIG-I/MAVS signaling. When co-expressed with bcTRAF2, bcTRIF-induced the transcription of interferon promoter in EPC cells was decreased, and the antiviral activity of bcTRIF was dampened accordingly. On the contrary, co-expressed bcTRAF6 enhanced both bcTRIF-mediated interferon promoter transcription and antiviral activity. The subsequent co-immunoprecipitation identified the interaction between bcTRAF2/6 and bcTRIF. Thus, bcTRIF-mediated antiviral signaling is up-regulated by bcTRAF6 and down-regulated by bcTRAF2.
•bcTRIF interacts with both bcTRAF2 and bcTRAF6.•bcTRIF-mediated antiviral signaling is positively regulated by bcTRAF6.•bcTRIF-mediated antiviral signaling is negatively regulated by bcTRAF2.</description><subject>Adaptor Proteins, Vesicular Transport - genetics</subject><subject>Adaptor Proteins, Vesicular Transport - metabolism</subject><subject>Animals</subject><subject>Antiviral activity</subject><subject>Black carp</subject><subject>Carp</subject><subject>Carps - genetics</subject><subject>Carps - immunology</subject><subject>Carps - virology</subject><subject>Cloning, Molecular</subject><subject>Down-Regulation - immunology</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - virology</subject><subject>Fish Proteins - genetics</subject><subject>Fish Proteins - metabolism</subject><subject>Homology</subject><subject>Immunoprecipitation</subject><subject>Interferon</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Signaling</subject><subject>TNF Receptor-Associated Factor 2 - metabolism</subject><subject>TNF Receptor-Associated Factor 6 - metabolism</subject><subject>Toll-like receptors</subject><subject>TRAF2</subject><subject>TRAF2 protein</subject><subject>TRAF6</subject><subject>TRAF6 protein</subject><subject>Transcription</subject><subject>TRIF</subject><subject>Up-Regulation - immunology</subject><subject>Viremia</subject><subject>Viruses</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEYRoMotl4ewI0E3LiZmstMJoOrUqwKgiAVXBkyuZTUdKYmM4W-vWmrLlyYTRJyvo_8B4ALjEYYYXazGGnlRgQRnO45KukBGGJeVhlCvDoEQ4TzIqOoeBuAkxgXKC2O0TEYUFoyVvByCN5nL4_TbGm0k53RUDadW7sgPYxu3kjvmjl0EWpnrQkmPUrvNzCYee93fL2Bs5fxlKSg3p0YdA2svVQfUMmwOgNHVvpozr_3U_A6vZtNHrKn5_vHyfgpU5TjLpNGK8sly0tbUF0ogjUjliKGSVEzwqni6e-WMkS5ZbjOKdOsTgFCCcoVpafget-7Cu1nb2Inli4q471sTNtHQQrESJWmrxJ69QddtH1Is24pWlU5ymmeKLynVGhjDMaKVXBLGTYCI7GVLxYiyRdb-WIvP2Uuv5v7Ohn9TfzYTsDtHjBJxdqZIKJyplHJfjCqE7p1_9R_ATgCkV0</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Peng, Fei</creator><creator>Jin, Saisai</creator><creator>Chen, Zhaoyuan</creator><creator>Chang, Haiyan</creator><creator>Xiao, Jun</creator><creator>Li, Jianzhong</creator><creator>Zou, Jun</creator><creator>Feng, Hao</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>202108</creationdate><title>TRIF-mediated antiviral signaling is differentially regulated by TRAF2 and TRAF6 in black carp</title><author>Peng, Fei ; Jin, Saisai ; Chen, Zhaoyuan ; Chang, Haiyan ; Xiao, Jun ; Li, Jianzhong ; Zou, Jun ; Feng, Hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-aedcf8a647f53d5c21d62f306125b6283c8000f36038f61b436d6b8a623204c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adaptor Proteins, Vesicular Transport - genetics</topic><topic>Adaptor Proteins, Vesicular Transport - metabolism</topic><topic>Animals</topic><topic>Antiviral activity</topic><topic>Black carp</topic><topic>Carp</topic><topic>Carps - genetics</topic><topic>Carps - immunology</topic><topic>Carps - virology</topic><topic>Cloning, Molecular</topic><topic>Down-Regulation - immunology</topic><topic>Fish Diseases - immunology</topic><topic>Fish Diseases - virology</topic><topic>Fish Proteins - genetics</topic><topic>Fish Proteins - metabolism</topic><topic>Homology</topic><topic>Immunoprecipitation</topic><topic>Interferon</topic><topic>Receptors, Immunologic - genetics</topic><topic>Receptors, Immunologic - metabolism</topic><topic>Signaling</topic><topic>TNF Receptor-Associated Factor 2 - metabolism</topic><topic>TNF Receptor-Associated Factor 6 - metabolism</topic><topic>Toll-like receptors</topic><topic>TRAF2</topic><topic>TRAF2 protein</topic><topic>TRAF6</topic><topic>TRAF6 protein</topic><topic>Transcription</topic><topic>TRIF</topic><topic>Up-Regulation - immunology</topic><topic>Viremia</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Fei</creatorcontrib><creatorcontrib>Jin, Saisai</creatorcontrib><creatorcontrib>Chen, Zhaoyuan</creatorcontrib><creatorcontrib>Chang, Haiyan</creatorcontrib><creatorcontrib>Xiao, Jun</creatorcontrib><creatorcontrib>Li, Jianzhong</creatorcontrib><creatorcontrib>Zou, Jun</creatorcontrib><creatorcontrib>Feng, Hao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Fei</au><au>Jin, Saisai</au><au>Chen, Zhaoyuan</au><au>Chang, Haiyan</au><au>Xiao, Jun</au><au>Li, Jianzhong</au><au>Zou, Jun</au><au>Feng, Hao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TRIF-mediated antiviral signaling is differentially regulated by TRAF2 and TRAF6 in black carp</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2021-08</date><risdate>2021</risdate><volume>121</volume><spage>104073</spage><epage>104073</epage><pages>104073-104073</pages><artnum>104073</artnum><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>TRIF is an antiviral adaptor downstream of Toll-like receptors, the roles of teleost TRIF and their regulation remain largely unknown. In this study, a TRIF homologue (bcTRIF) of black carp (Mylopharyngodon piceus) has been cloned, and the transcription of bcTRIF in vivo and ex vivo increased in response to different stimuli. Overexpressed bcTRIF induced the transcription of interferon promoter in the EPC cells and enhanced protection of cells against infection of spring viremia of carp virus (SVCV). The previous study has identified that black carp TRAF2 (bcTRAF2) and TRAF6 (bcTRAF6) functioned positively in RIG-I/MAVS signaling. When co-expressed with bcTRAF2, bcTRIF-induced the transcription of interferon promoter in EPC cells was decreased, and the antiviral activity of bcTRIF was dampened accordingly. On the contrary, co-expressed bcTRAF6 enhanced both bcTRIF-mediated interferon promoter transcription and antiviral activity. The subsequent co-immunoprecipitation identified the interaction between bcTRAF2/6 and bcTRIF. Thus, bcTRIF-mediated antiviral signaling is up-regulated by bcTRAF6 and down-regulated by bcTRAF2.
•bcTRIF interacts with both bcTRAF2 and bcTRAF6.•bcTRIF-mediated antiviral signaling is positively regulated by bcTRAF6.•bcTRIF-mediated antiviral signaling is negatively regulated by bcTRAF2.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>33766587</pmid><doi>10.1016/j.dci.2021.104073</doi><tpages>1</tpages></addata></record> |
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| subjects | Adaptor Proteins, Vesicular Transport - genetics Adaptor Proteins, Vesicular Transport - metabolism Animals Antiviral activity Black carp Carp Carps - genetics Carps - immunology Carps - virology Cloning, Molecular Down-Regulation - immunology Fish Diseases - immunology Fish Diseases - virology Fish Proteins - genetics Fish Proteins - metabolism Homology Immunoprecipitation Interferon Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Signaling TNF Receptor-Associated Factor 2 - metabolism TNF Receptor-Associated Factor 6 - metabolism Toll-like receptors TRAF2 TRAF2 protein TRAF6 TRAF6 protein Transcription TRIF Up-Regulation - immunology Viremia Viruses |
| title | TRIF-mediated antiviral signaling is differentially regulated by TRAF2 and TRAF6 in black carp |
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