Plant-produced bacteriocins inhibit plant pathogens and confer disease resistance in tomato

[Display omitted] •Bacterial antimicrobial peptides (bacteriocins) were produced in planta.•Plant extracts inhibited growth of food and plant pathogens in vitro.•Plantaricin and leucocin-expressing tomato plants were disease resistant. Bacteriocins are a diverse group of bacterial antimicrobial pept...

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Veröffentlicht in:New biotechnology 2021-07, Vol.63, p.54-61
Hauptverfasser: Mirzaee, Hooman, Neira Peralta, Noelia L., Carvalhais, Lilia C., Dennis, Paul G., Schenk, Peer M.
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container_issue
container_start_page 54
container_title New biotechnology
container_volume 63
creator Mirzaee, Hooman
Neira Peralta, Noelia L.
Carvalhais, Lilia C.
Dennis, Paul G.
Schenk, Peer M.
description [Display omitted] •Bacterial antimicrobial peptides (bacteriocins) were produced in planta.•Plant extracts inhibited growth of food and plant pathogens in vitro.•Plantaricin and leucocin-expressing tomato plants were disease resistant. Bacteriocins are a diverse group of bacterial antimicrobial peptides (AMPs) that represent potential replacements for current antibiotics due to their novel modes of action. At present, production costs are a key constraint to the use of bacteriocins and other AMPs. Here, we report the production of bacteriocins in planta – a potentially scalable and cost-effective approach for AMP production. Nine bacteriocin genes with three different modes of action and minimal or no post-translational modifications were synthesized, cloned and used to transform Arabidopsis thaliana. To confirm bacteriocin functionality and the potential to use these plants as biofactories, Arabidopsis T3 crude leaf extracts were subjected to inhibition assays against the bacterial pathogens Clavibacter michiganensis subsp. michiganensis (Cmm) and Pseudomonas syringae pv. tomato DC3000 (Pst). Six and seven of nine extracts significantly inhibited Cmm and Pst, respectively. Three bacteriocin genes (plantaricin, enteriocin, and leucocin) were then selected for over-expression in tomato (Solanum lycopersicum). In vitro plant pathogen inhibition assays of T0, T1 and T2 transgenic tomato leaf extracts confirmed antimicrobial activity against both pathogens for all three generations of plants, indicating their potential use as stable biopesticide biofactories. Plantaricin and leucocin-expressing T2 tomato plants were resistant to Cmm, and leucocin-expressing T2 plants were resistant to Pst. This study highlights that plants can be used as biofactories for AMP production and that the expression of bacteriocins in planta may offer new opportunities for disease control in agriculture.
doi_str_mv 10.1016/j.nbt.2021.03.003
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Bacteriocins are a diverse group of bacterial antimicrobial peptides (AMPs) that represent potential replacements for current antibiotics due to their novel modes of action. At present, production costs are a key constraint to the use of bacteriocins and other AMPs. Here, we report the production of bacteriocins in planta – a potentially scalable and cost-effective approach for AMP production. Nine bacteriocin genes with three different modes of action and minimal or no post-translational modifications were synthesized, cloned and used to transform Arabidopsis thaliana. To confirm bacteriocin functionality and the potential to use these plants as biofactories, Arabidopsis T3 crude leaf extracts were subjected to inhibition assays against the bacterial pathogens Clavibacter michiganensis subsp. michiganensis (Cmm) and Pseudomonas syringae pv. tomato DC3000 (Pst). Six and seven of nine extracts significantly inhibited Cmm and Pst, respectively. Three bacteriocin genes (plantaricin, enteriocin, and leucocin) were then selected for over-expression in tomato (Solanum lycopersicum). In vitro plant pathogen inhibition assays of T0, T1 and T2 transgenic tomato leaf extracts confirmed antimicrobial activity against both pathogens for all three generations of plants, indicating their potential use as stable biopesticide biofactories. Plantaricin and leucocin-expressing T2 tomato plants were resistant to Cmm, and leucocin-expressing T2 plants were resistant to Pst. 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Bacteriocins are a diverse group of bacterial antimicrobial peptides (AMPs) that represent potential replacements for current antibiotics due to their novel modes of action. At present, production costs are a key constraint to the use of bacteriocins and other AMPs. Here, we report the production of bacteriocins in planta – a potentially scalable and cost-effective approach for AMP production. Nine bacteriocin genes with three different modes of action and minimal or no post-translational modifications were synthesized, cloned and used to transform Arabidopsis thaliana. To confirm bacteriocin functionality and the potential to use these plants as biofactories, Arabidopsis T3 crude leaf extracts were subjected to inhibition assays against the bacterial pathogens Clavibacter michiganensis subsp. michiganensis (Cmm) and Pseudomonas syringae pv. tomato DC3000 (Pst). Six and seven of nine extracts significantly inhibited Cmm and Pst, respectively. 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subjects Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antimicrobial peptide
Antimicrobial peptides
Arabidopsis thaliana
Bacteriocin
Bacteriocins
Biofactory
Clavibacter michiganensis michiganensis
Disease control
Disease resistance
Genes
Leaves
Overexpression
Pathogens
Peptides
Pesticides
Plant bacterial diseases
Plant diseases
Plant extracts
Plant pathogens
Plant transformation
Plants
Post-translation
Production costs
Pseudomonas syringae
Solanum lycopersicum
Tomatoes
Transgenic plants
title Plant-produced bacteriocins inhibit plant pathogens and confer disease resistance in tomato
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