Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents
Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 ph...
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| Veröffentlicht in: | Clinical immunology (Orlando, Fla.) Fla.), 2021-08, Vol.229, p.108715-108715, Article 108715 |
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| creator | Leija-Martínez, José J. Del-Río-Navarro, Blanca E. Sanchéz-Muñoz, Fausto Muñoz-Hernández, Onofre Hong, Enrique Giacoman-Martínez, Abraham Romero-Nava, Rodrigo Patricio-Román, Karla L. Hall-Mondragon, Margareth S. Espinosa-Velazquez, Dario Villafaña, Santiago Huang, Fengyang |
| description | Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma. |
| doi_str_mv | 10.1016/j.clim.2021.108715 |
| format | Article |
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Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). 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Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.</description><subject>Asthma</subject><subject>IL-17A</subject><subject>Non-allergic asthma</subject><subject>Obesity</subject><subject>RORC</subject><subject>Tumour necrosis factor-alpha</subject><issn>1521-6616</issn><issn>1521-7035</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v2zAMho2iA_qx_oGedNyhzvRhyS7QSxA0W4GgBYLuLDAyjSiTLVdU2uUP7HfPQXreiQT5PgTxFMWt4DPBhfm-m7ng-5nkUkyDphb6rLgUWoqy5kqff_bGCHNRXBHtOOdaSnNZ_J0TRech-zgQix17fV7O79jTStRTgaFl65f1gvXr5znDP2NCoinJAr5jIOYHNmLy4xYTBLYJMbbTav87ukNGYh8-b1ncIPl8KBMGyNgyoLzt4YhCGwOSwyHT1-JLB4Hw5rNeF7-Wj6-Ln-Xq5cfTYr4qnVIqlw1KbFqoFGqh6tqZioNGqJ3QgA2XDsBsTGVce6-ajek4iPtON7wyWhmoUF0X3053xxTf9kjZ9n76IAQYMO7JSs2NrJuqMVNUnqIuRaKEnR2T7yEdrOD2KN3u7FG6PUq3J-kT9HCCJjv47jFZch4Hh61P6LJto_8f_g-nV4tG</recordid><startdate>202108</startdate><enddate>202108</enddate><creator>Leija-Martínez, José J.</creator><creator>Del-Río-Navarro, Blanca E.</creator><creator>Sanchéz-Muñoz, Fausto</creator><creator>Muñoz-Hernández, Onofre</creator><creator>Hong, Enrique</creator><creator>Giacoman-Martínez, Abraham</creator><creator>Romero-Nava, Rodrigo</creator><creator>Patricio-Román, Karla L.</creator><creator>Hall-Mondragon, Margareth S.</creator><creator>Espinosa-Velazquez, Dario</creator><creator>Villafaña, Santiago</creator><creator>Huang, Fengyang</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202108</creationdate><title>Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents</title><author>Leija-Martínez, José J. ; Del-Río-Navarro, Blanca E. ; Sanchéz-Muñoz, Fausto ; Muñoz-Hernández, Onofre ; Hong, Enrique ; Giacoman-Martínez, Abraham ; Romero-Nava, Rodrigo ; Patricio-Román, Karla L. ; Hall-Mondragon, Margareth S. ; Espinosa-Velazquez, Dario ; Villafaña, Santiago ; Huang, Fengyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-8e2e8da43e51377c640a5ea7c15ae802caa6b646cd938b6f0a19f58046536a4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Asthma</topic><topic>IL-17A</topic><topic>Non-allergic asthma</topic><topic>Obesity</topic><topic>RORC</topic><topic>Tumour necrosis factor-alpha</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leija-Martínez, José J.</creatorcontrib><creatorcontrib>Del-Río-Navarro, Blanca E.</creatorcontrib><creatorcontrib>Sanchéz-Muñoz, Fausto</creatorcontrib><creatorcontrib>Muñoz-Hernández, Onofre</creatorcontrib><creatorcontrib>Hong, Enrique</creatorcontrib><creatorcontrib>Giacoman-Martínez, Abraham</creatorcontrib><creatorcontrib>Romero-Nava, Rodrigo</creatorcontrib><creatorcontrib>Patricio-Román, Karla L.</creatorcontrib><creatorcontrib>Hall-Mondragon, Margareth S.</creatorcontrib><creatorcontrib>Espinosa-Velazquez, Dario</creatorcontrib><creatorcontrib>Villafaña, Santiago</creatorcontrib><creatorcontrib>Huang, Fengyang</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical immunology (Orlando, Fla.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leija-Martínez, José J.</au><au>Del-Río-Navarro, Blanca E.</au><au>Sanchéz-Muñoz, Fausto</au><au>Muñoz-Hernández, Onofre</au><au>Hong, Enrique</au><au>Giacoman-Martínez, Abraham</au><au>Romero-Nava, Rodrigo</au><au>Patricio-Román, Karla L.</au><au>Hall-Mondragon, Margareth S.</au><au>Espinosa-Velazquez, Dario</au><au>Villafaña, Santiago</au><au>Huang, Fengyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents</atitle><jtitle>Clinical immunology (Orlando, Fla.)</jtitle><date>2021-08</date><risdate>2021</risdate><volume>229</volume><spage>108715</spage><epage>108715</epage><pages>108715-108715</pages><artnum>108715</artnum><issn>1521-6616</issn><eissn>1521-7035</eissn><abstract>Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.clim.2021.108715</doi><tpages>1</tpages></addata></record> |
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| subjects | Asthma IL-17A Non-allergic asthma Obesity RORC Tumour necrosis factor-alpha |
| title | Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents |
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