Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome

Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome

Purpose The present study aimed to explore the associations between the expression pattern of molecules in the Notch pathway in the cumulus cells of polycystic ovary syndrome ( PCOS ) patients and the quality of zygotes and embryos . Methods A total of 200 cumulus complexes surrounding mature oocyte...

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Veröffentlicht in:Archives of gynecology and obstetrics 2021-10, Vol.304 (4), p.1097-1105
Hauptverfasser: Masoudi, Mojtaba, Yamini, Nazila, Salehi, Fahimeh, Aflatoonian, Reza, Kutenaee, Maryam Azizi, Esfandiyari, Sahar, Hosseini, Elham, Asadi, Ebrahim, Najarkolaei, Azadeh Hosseini, Mortezaee, Keywan, Moini, Ashraf, Abolhassani, Farid, Salehi, Ensieh
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Embryos
Endocrinology
Gynecologic Endocrinology and Reproductive Medicine
Gynecology
Human Genetics
Medicine
Medicine & Public Health
Obstetrics/Perinatology/Midwifery
Ovaries
Polycystic ovary syndrome
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container_issue 4
container_start_page 1097
container_title Archives of gynecology and obstetrics
container_volume 304
creator Masoudi, Mojtaba
Yamini, Nazila
Salehi, Fahimeh
Aflatoonian, Reza
Kutenaee, Maryam Azizi
Esfandiyari, Sahar
Hosseini, Elham
Asadi, Ebrahim
Najarkolaei, Azadeh Hosseini
Mortezaee, Keywan
Moini, Ashraf
Abolhassani, Farid
Salehi, Ensieh
description Purpose The present study aimed to explore the associations between the expression pattern of molecules in the Notch pathway in the cumulus cells of polycystic ovary syndrome ( PCOS ) patients and the quality of zygotes and embryos . Methods A total of 200 cumulus complexes surrounding mature oocytes were obtained from 40 patients with and without PCOS undergoing intracytoplasmic sperm injection (ICSI). The expressions of Notch- 1, Notch- 2, and Notch -3 genes were examined by Reverse Transcription Q-PCR assay. Moreover, immunocytochemistry was performed for the expressions of Jagged-1 and Jagged-2 proteins. The correlations between the Notch receptors and their ligand expressions and the qualities of the zygote and embryo were investigated. Results The expression levels of Notch -2, Notch -3 , Jagged-1, and Jagged-2 were significantly lower in patients with PCOS than in normal women ( p  
doi_str_mv 10.1007/s00404-021-06039-1
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Methods A total of 200 cumulus complexes surrounding mature oocytes were obtained from 40 patients with and without PCOS undergoing intracytoplasmic sperm injection (ICSI). The expressions of Notch- 1, Notch- 2, and Notch -3 genes were examined by Reverse Transcription Q-PCR assay. Moreover, immunocytochemistry was performed for the expressions of Jagged-1 and Jagged-2 proteins. The correlations between the Notch receptors and their ligand expressions and the qualities of the zygote and embryo were investigated. Results The expression levels of Notch -2, Notch -3 , Jagged-1, and Jagged-2 were significantly lower in patients with PCOS than in normal women ( p  &lt; 0.05), while Notch- 1 showed no meaningful difference between the groups. A positive correlation was found between Notch- 1 and embryo quality. Furthermore, only Notch- 2 and Jagged-2 marginally correlated with zygote quality. Conclusion The data of the present study indicated that evaluating the molecules in the Notch pathway in PCOS patients’ cumulus cells provides a novel approach to predict the zygote and embryo quality . However, further studies on a larger population are needed to validate this finding.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-021-06039-1</identifier><identifier>PMID: 33772329</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Embryos ; Endocrinology ; Gynecologic Endocrinology and Reproductive Medicine ; Gynecology ; Human Genetics ; Medicine ; Medicine &amp; Public Health ; Obstetrics/Perinatology/Midwifery ; Ovaries ; Polycystic ovary syndrome</subject><ispartof>Archives of gynecology and obstetrics, 2021-10, Vol.304 (4), p.1097-1105</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-51bd49090a3451b8dff6e944796f3f03597efb9d2e79c1c5e11c5533d63e3bda3</citedby><cites>FETCH-LOGICAL-c441t-51bd49090a3451b8dff6e944796f3f03597efb9d2e79c1c5e11c5533d63e3bda3</cites><orcidid>0000-0002-1297-1774</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-021-06039-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-021-06039-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33772329$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masoudi, Mojtaba</creatorcontrib><creatorcontrib>Yamini, Nazila</creatorcontrib><creatorcontrib>Salehi, Fahimeh</creatorcontrib><creatorcontrib>Aflatoonian, Reza</creatorcontrib><creatorcontrib>Kutenaee, Maryam Azizi</creatorcontrib><creatorcontrib>Esfandiyari, Sahar</creatorcontrib><creatorcontrib>Hosseini, Elham</creatorcontrib><creatorcontrib>Asadi, Ebrahim</creatorcontrib><creatorcontrib>Najarkolaei, Azadeh Hosseini</creatorcontrib><creatorcontrib>Mortezaee, Keywan</creatorcontrib><creatorcontrib>Moini, Ashraf</creatorcontrib><creatorcontrib>Abolhassani, Farid</creatorcontrib><creatorcontrib>Salehi, Ensieh</creatorcontrib><title>Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Purpose The present study aimed to explore the associations between the expression pattern of molecules in the Notch pathway in the cumulus cells of polycystic ovary syndrome ( PCOS ) patients and the quality of zygotes and embryos . Methods A total of 200 cumulus complexes surrounding mature oocytes were obtained from 40 patients with and without PCOS undergoing intracytoplasmic sperm injection (ICSI). The expressions of Notch- 1, Notch- 2, and Notch -3 genes were examined by Reverse Transcription Q-PCR assay. Moreover, immunocytochemistry was performed for the expressions of Jagged-1 and Jagged-2 proteins. The correlations between the Notch receptors and their ligand expressions and the qualities of the zygote and embryo were investigated. Results The expression levels of Notch -2, Notch -3 , Jagged-1, and Jagged-2 were significantly lower in patients with PCOS than in normal women ( p  &lt; 0.05), while Notch- 1 showed no meaningful difference between the groups. A positive correlation was found between Notch- 1 and embryo quality. Furthermore, only Notch- 2 and Jagged-2 marginally correlated with zygote quality. Conclusion The data of the present study indicated that evaluating the molecules in the Notch pathway in PCOS patients’ cumulus cells provides a novel approach to predict the zygote and embryo quality . 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Methods A total of 200 cumulus complexes surrounding mature oocytes were obtained from 40 patients with and without PCOS undergoing intracytoplasmic sperm injection (ICSI). The expressions of Notch- 1, Notch- 2, and Notch -3 genes were examined by Reverse Transcription Q-PCR assay. Moreover, immunocytochemistry was performed for the expressions of Jagged-1 and Jagged-2 proteins. The correlations between the Notch receptors and their ligand expressions and the qualities of the zygote and embryo were investigated. Results The expression levels of Notch -2, Notch -3 , Jagged-1, and Jagged-2 were significantly lower in patients with PCOS than in normal women ( p  &lt; 0.05), while Notch- 1 showed no meaningful difference between the groups. A positive correlation was found between Notch- 1 and embryo quality. Furthermore, only Notch- 2 and Jagged-2 marginally correlated with zygote quality. Conclusion The data of the present study indicated that evaluating the molecules in the Notch pathway in PCOS patients’ cumulus cells provides a novel approach to predict the zygote and embryo quality . However, further studies on a larger population are needed to validate this finding.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33772329</pmid><doi>10.1007/s00404-021-06039-1</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1297-1774</orcidid></addata></record>
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subjects Embryos
Endocrinology
Gynecologic Endocrinology and Reproductive Medicine
Gynecology
Human Genetics
Medicine
Medicine & Public Health
Obstetrics/Perinatology/Midwifery
Ovaries
Polycystic ovary syndrome
title Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome
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