Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm
In the present investigation, we have evaluated the antibiofilm potential of Bacillus licheniformis SV1 derived glycolipid against C. glabrata biofilm. Impact of isolated glycolipid on the viability of C. glabrata and on inhibiting as well as eradicating ability of its biofilm were studied. Further,...
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| Veröffentlicht in: | Current microbiology 2021-05, Vol.78 (5), p.1813-1822 |
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| creator | Gupta, Sonam Gupta, Payal Pruthi, Vikas |
| description | In the present investigation, we have evaluated the antibiofilm potential of
Bacillus licheniformis
SV1 derived glycolipid against
C. glabrata
biofilm. Impact of isolated glycolipid on the viability of
C. glabrata
and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of
C. glabrata
in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml
−1
) inhibited and eradicated
C. glabrata
biofilm approximately 80% and 60%, respectively. FE-SEM images revealed glycolipid exposure results in architectural alteration and eradication of
C. glabrata
biofilm and ROS generation. Transcriptional studies of selected genes showed that the expression of
AUS1
,
FKS1
and
KRE1
were down-regulated, while that of ergosterol biosynthesis pathway and multidrug transporter increased, in the presence of glycolipid. |
| doi_str_mv | 10.1007/s00284-021-02461-5 |
| format | Article |
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Bacillus licheniformis
SV1 derived glycolipid against
C. glabrata
biofilm. Impact of isolated glycolipid on the viability of
C. glabrata
and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of
C. glabrata
in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml
−1
) inhibited and eradicated
C. glabrata
biofilm approximately 80% and 60%, respectively. FE-SEM images revealed glycolipid exposure results in architectural alteration and eradication of
C. glabrata
biofilm and ROS generation. Transcriptional studies of selected genes showed that the expression of
AUS1
,
FKS1
and
KRE1
were down-regulated, while that of ergosterol biosynthesis pathway and multidrug transporter increased, in the presence of glycolipid.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-021-02461-5</identifier><identifier>PMID: 33772618</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antifungal Agents ; Bacillus licheniformis ; Biofilms ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Candida glabrata - genetics ; Ergosterol ; Gene expression ; Genes ; Glycolipids - pharmacology ; Life Sciences ; Microbiology ; Polymerase chain reaction ; Reactive oxygen species ; Transcription</subject><ispartof>Current microbiology, 2021-05, Vol.78 (5), p.1813-1822</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-af02fcf2283b7c93513bfdfd545c305d5f535f315b148251176bc1fcf34adcd63</citedby><cites>FETCH-LOGICAL-c375t-af02fcf2283b7c93513bfdfd545c305d5f535f315b148251176bc1fcf34adcd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-021-02461-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-021-02461-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33772618$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gupta, Sonam</creatorcontrib><creatorcontrib>Gupta, Payal</creatorcontrib><creatorcontrib>Pruthi, Vikas</creatorcontrib><title>Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>In the present investigation, we have evaluated the antibiofilm potential of
Bacillus licheniformis
SV1 derived glycolipid against
C. glabrata
biofilm. Impact of isolated glycolipid on the viability of
C. glabrata
and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of
C. glabrata
in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml
−1
) inhibited and eradicated
C. glabrata
biofilm approximately 80% and 60%, respectively. FE-SEM images revealed glycolipid exposure results in architectural alteration and eradication of
C. glabrata
biofilm and ROS generation. Transcriptional studies of selected genes showed that the expression of
AUS1
,
FKS1
and
KRE1
were down-regulated, while that of ergosterol biosynthesis pathway and multidrug transporter increased, in the presence of glycolipid.</description><subject>Antifungal Agents</subject><subject>Bacillus licheniformis</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Candida glabrata - genetics</subject><subject>Ergosterol</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glycolipids - pharmacology</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Polymerase chain reaction</subject><subject>Reactive oxygen species</subject><subject>Transcription</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kEtPGzEURq0KVAL0D7CoLLHpZsDXj3ksm9BCJCQWKd1aHj-CkWec2hmk_HvcJlCpiy6su_D5vnt1ELoAcgWENNeZENryilAoj9dQiQ9oBpzRinQdHKEZYZxVbS3gBJ3m_EwI0I7AR3TCWNPQGtoZWi2HjdJbHB2eK-1DmDIOXj_Z0buYBp_x6ifgG5v8izX4Nux0DH7jDY4jXqjReKPwOqg-qa3Ccx-dD8M5OnYqZPvpMM_Q4_dvPxZ31f3D7XLx9b7SrBHbSjlCnXaUtqxvdMcEsN4ZZwQXmhFhhBNMOAaiB95SAdDUvYaSYFwZbWp2hr7sezcp_pps3spyr7YhqNHGKUsqSE0bTjgU9PIf9DlOaSzXFQo6UbctawtF95ROMedkndwkP6i0k0Dkb-Vyr1wW5fKPcilK6POheuoHa94jb44LwPZALl_j2qa_u_9T-wrnE4qh</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Gupta, Sonam</creator><creator>Gupta, Payal</creator><creator>Pruthi, Vikas</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20210501</creationdate><title>Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm</title><author>Gupta, Sonam ; 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Bacillus licheniformis
SV1 derived glycolipid against
C. glabrata
biofilm. Impact of isolated glycolipid on the viability of
C. glabrata
and on inhibiting as well as eradicating ability of its biofilm were studied. Further, morphological alterations, reactive oxygen species generation (ROS) production and transcriptional expression of selected genes (RT-PCR) of
C. glabrata
in response with isolated glycolipid were studied. The isolated glycolipid (1.0 mg ml
−1
) inhibited and eradicated
C. glabrata
biofilm approximately 80% and 60%, respectively. FE-SEM images revealed glycolipid exposure results in architectural alteration and eradication of
C. glabrata
biofilm and ROS generation. Transcriptional studies of selected genes showed that the expression of
AUS1
,
FKS1
and
KRE1
were down-regulated, while that of ergosterol biosynthesis pathway and multidrug transporter increased, in the presence of glycolipid.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33772618</pmid><doi>10.1007/s00284-021-02461-5</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 2021-05, Vol.78 (5), p.1813-1822 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Antifungal Agents Bacillus licheniformis Biofilms Biomedical and Life Sciences Biosynthesis Biotechnology Candida glabrata - genetics Ergosterol Gene expression Genes Glycolipids - pharmacology Life Sciences Microbiology Polymerase chain reaction Reactive oxygen species Transcription |
| title | Impact of Bacillus licheniformis SV1 Derived Glycolipid on Candida glabrata Biofilm |
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