Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period
Problem Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cell...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2022-01, Vol.87 (1), p.e13423-n/a |
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| container_title | American journal of reproductive immunology (1989) |
| container_volume | 87 |
| creator | Fernández, Laura Grasso, Esteban Soczewski, Elizabeth Gori, Soledad Calo, Guillermina Hauk, Vanesa Sabbione, Florencia Gallino, Lucila Martínez, Gustavo Irigoyen, Marcela Bestach, Yesica Pérez Leirós, Claudia Ramhorst, Rosanna |
| description | Problem
Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu.
Method of Study
We used an in vitro model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl‐cAMP, then treated with human blastocysts‐conditioned media (BCM) classified according to their quality.
Results
Decidualized cells treated with BCM from impaired developed blastocysts increased IL‐1β production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts.
Conclusion
Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo‐endometrial dialogue. |
| doi_str_mv | 10.1111/aji.13423 |
| format | Article |
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Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu.
Method of Study
We used an in vitro model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl‐cAMP, then treated with human blastocysts‐conditioned media (BCM) classified according to their quality.
Results
Decidualized cells treated with BCM from impaired developed blastocysts increased IL‐1β production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts.
Conclusion
Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo‐endometrial dialogue.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.13423</identifier><identifier>PMID: 33764560</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Blastocyst - drug effects ; Blastocyst - physiology ; Blastocysts ; Cell activation ; Cell differentiation ; Cell Line ; Chemokines ; CXCL12 protein ; Decidua - drug effects ; Decidua - metabolism ; decidualization ; Embryo Implantation - drug effects ; Embryo Implantation - physiology ; embryo quality ; Embryos ; Endometrium ; Female ; Humans ; Immunoregulation ; implantation window ; Inflammation ; Inflammation - metabolism ; Leukocytes (neutrophilic) ; Lymphocytes T ; Medroxyprogesterone - administration & dosage ; Menstruation ; Microenvironments ; Natural selection ; Neutrophils ; Stromal cells ; Stromal Cells - drug effects ; Stromal Cells - metabolism</subject><ispartof>American journal of reproductive immunology (1989), 2022-01, Vol.87 (1), p.e13423-n/a</ispartof><rights>2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-10abf4bd382283d2cab4751267de4bcc814c8f80c4de036dbd3f44104e126cc23</citedby><cites>FETCH-LOGICAL-c3533-10abf4bd382283d2cab4751267de4bcc814c8f80c4de036dbd3f44104e126cc23</cites><orcidid>0000-0003-4222-817X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.13423$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.13423$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33764560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernández, Laura</creatorcontrib><creatorcontrib>Grasso, Esteban</creatorcontrib><creatorcontrib>Soczewski, Elizabeth</creatorcontrib><creatorcontrib>Gori, Soledad</creatorcontrib><creatorcontrib>Calo, Guillermina</creatorcontrib><creatorcontrib>Hauk, Vanesa</creatorcontrib><creatorcontrib>Sabbione, Florencia</creatorcontrib><creatorcontrib>Gallino, Lucila</creatorcontrib><creatorcontrib>Martínez, Gustavo</creatorcontrib><creatorcontrib>Irigoyen, Marcela</creatorcontrib><creatorcontrib>Bestach, Yesica</creatorcontrib><creatorcontrib>Pérez Leirós, Claudia</creatorcontrib><creatorcontrib>Ramhorst, Rosanna</creatorcontrib><title>Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu.
Method of Study
We used an in vitro model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl‐cAMP, then treated with human blastocysts‐conditioned media (BCM) classified according to their quality.
Results
Decidualized cells treated with BCM from impaired developed blastocysts increased IL‐1β production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts.
Conclusion
Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo‐endometrial dialogue.</description><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>Blastocysts</subject><subject>Cell activation</subject><subject>Cell differentiation</subject><subject>Cell Line</subject><subject>Chemokines</subject><subject>CXCL12 protein</subject><subject>Decidua - drug effects</subject><subject>Decidua - metabolism</subject><subject>decidualization</subject><subject>Embryo Implantation - drug effects</subject><subject>Embryo Implantation - physiology</subject><subject>embryo quality</subject><subject>Embryos</subject><subject>Endometrium</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoregulation</subject><subject>implantation window</subject><subject>Inflammation</subject><subject>Inflammation - metabolism</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lymphocytes T</subject><subject>Medroxyprogesterone - administration & dosage</subject><subject>Menstruation</subject><subject>Microenvironments</subject><subject>Natural selection</subject><subject>Neutrophils</subject><subject>Stromal cells</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - metabolism</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1TAQhi1ERUthwQsgS2xgkdaO7SSwKxWXVpXY0LXl2BPqI19S2xHKjkdAPCJPgs85bRdInY1Ho0_fjPwj9IqSE1rrVG3sCWW8ZU_QEe0Iacjwvn9ae8K7pudkOETPc94QUuesf4YOGes7LjpyhP5cBwMpFxWMDT9wuQEcVFmScjiDA11sDDhO-GbxKmDwY1pj_oA_OpVL1Gsu-HZRzpYV-2gWpwrkncSGySnvVYlpxQnyHEMGbJZ0v2WGZP_--m397FQoardnO4vmBTqYlMvw8u49RtefP30__9pcfftycX521WgmGGsoUePER8OGth2YabUaeS9o2_UG-Kj1QLkepoFoboCwzlRy4rx-CVRG65Ydo7d775zi7QK5SG-zBlfvgbhk2QoiWCcI26Jv_kM3cUmhXifbjvKeUyFEpd7tKZ1izgkmOSfrVVolJXIblKxByV1QlX19Z1xGD-aBvE-mAqd74Kd1sD5ukmeXF3vlP-4MoQc</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Fernández, Laura</creator><creator>Grasso, Esteban</creator><creator>Soczewski, Elizabeth</creator><creator>Gori, Soledad</creator><creator>Calo, Guillermina</creator><creator>Hauk, Vanesa</creator><creator>Sabbione, Florencia</creator><creator>Gallino, Lucila</creator><creator>Martínez, Gustavo</creator><creator>Irigoyen, Marcela</creator><creator>Bestach, Yesica</creator><creator>Pérez Leirós, Claudia</creator><creator>Ramhorst, Rosanna</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4222-817X</orcidid></search><sort><creationdate>202201</creationdate><title>Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period</title><author>Fernández, Laura ; Grasso, Esteban ; Soczewski, Elizabeth ; Gori, Soledad ; Calo, Guillermina ; Hauk, Vanesa ; Sabbione, Florencia ; Gallino, Lucila ; Martínez, Gustavo ; Irigoyen, Marcela ; Bestach, Yesica ; Pérez Leirós, Claudia ; Ramhorst, Rosanna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-10abf4bd382283d2cab4751267de4bcc814c8f80c4de036dbd3f44104e126cc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Blastocyst - drug effects</topic><topic>Blastocyst - physiology</topic><topic>Blastocysts</topic><topic>Cell activation</topic><topic>Cell differentiation</topic><topic>Cell Line</topic><topic>Chemokines</topic><topic>CXCL12 protein</topic><topic>Decidua - drug effects</topic><topic>Decidua - metabolism</topic><topic>decidualization</topic><topic>Embryo Implantation - drug effects</topic><topic>Embryo Implantation - physiology</topic><topic>embryo quality</topic><topic>Embryos</topic><topic>Endometrium</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoregulation</topic><topic>implantation window</topic><topic>Inflammation</topic><topic>Inflammation - metabolism</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lymphocytes T</topic><topic>Medroxyprogesterone - administration & dosage</topic><topic>Menstruation</topic><topic>Microenvironments</topic><topic>Natural selection</topic><topic>Neutrophils</topic><topic>Stromal cells</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández, Laura</creatorcontrib><creatorcontrib>Grasso, Esteban</creatorcontrib><creatorcontrib>Soczewski, Elizabeth</creatorcontrib><creatorcontrib>Gori, Soledad</creatorcontrib><creatorcontrib>Calo, Guillermina</creatorcontrib><creatorcontrib>Hauk, Vanesa</creatorcontrib><creatorcontrib>Sabbione, Florencia</creatorcontrib><creatorcontrib>Gallino, Lucila</creatorcontrib><creatorcontrib>Martínez, Gustavo</creatorcontrib><creatorcontrib>Irigoyen, Marcela</creatorcontrib><creatorcontrib>Bestach, Yesica</creatorcontrib><creatorcontrib>Pérez Leirós, Claudia</creatorcontrib><creatorcontrib>Ramhorst, Rosanna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández, Laura</au><au>Grasso, Esteban</au><au>Soczewski, Elizabeth</au><au>Gori, Soledad</au><au>Calo, Guillermina</au><au>Hauk, Vanesa</au><au>Sabbione, Florencia</au><au>Gallino, Lucila</au><au>Martínez, Gustavo</au><au>Irigoyen, Marcela</au><au>Bestach, Yesica</au><au>Pérez Leirós, Claudia</au><au>Ramhorst, Rosanna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2022-01</date><risdate>2022</risdate><volume>87</volume><issue>1</issue><spage>e13423</spage><epage>n/a</epage><pages>e13423-n/a</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
Decidualized cells display an active role during embryo implantation sensing blastocyst quality, allowing the implantation of normal developed blastocysts and preventing the invasion of impaired developed ones. Here, we characterized the immune microenvironment generated by decidualized cells in response to soluble factors secreted by blastocysts that shape the receptive milieu.
Method of Study
We used an in vitro model of decidualization based on the Human Endometrial Stromal Cells line (HESC) differentiated with medroxiprogesterone and dibutyryl‐cAMP, then treated with human blastocysts‐conditioned media (BCM) classified according to their quality.
Results
Decidualized cells treated with BCM from impaired developed blastocysts increased IL‐1β production. Next, we evaluated the ability of decidualized cells to modulate other mediators associated with menstruation as chemokines. Decidualized cells responded to stimulation with BCM from impaired developed blastocysts increasing CXCL12 expression and CXCL8 secretion. The modulation of these markers was associated with the recruitment and activation of neutrophils, while regulatory T cells recruitment was restrained. These changes were not observed in the presence of BCM from normal developed blastocysts.
Conclusion
Soluble factors released by impaired developed blastocysts induce an exacerbated inflammatory response associated with neutrophils recruitment and activation, providing new clues to understand the molecular basis of the embryo‐endometrial dialogue.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33764560</pmid><doi>10.1111/aji.13423</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4222-817X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2022-01, Vol.87 (1), p.e13423-n/a |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Blastocyst - drug effects Blastocyst - physiology Blastocysts Cell activation Cell differentiation Cell Line Chemokines CXCL12 protein Decidua - drug effects Decidua - metabolism decidualization Embryo Implantation - drug effects Embryo Implantation - physiology embryo quality Embryos Endometrium Female Humans Immunoregulation implantation window Inflammation Inflammation - metabolism Leukocytes (neutrophilic) Lymphocytes T Medroxyprogesterone - administration & dosage Menstruation Microenvironments Natural selection Neutrophils Stromal cells Stromal Cells - drug effects Stromal Cells - metabolism |
| title | Understanding the natural selection of human embryos: Blastocyst quality modulates the inflammatory response during the peri‐implantation period |
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