Calibration for quantitative Fc-glycosylation analysis of therapeutic IgG1-type monoclonal antibodies by using glycopeptide standards

Fc-glycosylation has crucial impact on the efficacy and safety of IgG-type therapeutic monoclonal antibodies (mAbs). In order to enhance the performance of MS-based bottom-up quantitation strategy, a library of glycopeptide standards containing 26 common IgG1-type Fc-glycoforms has been constructed...

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Veröffentlicht in:	Analytica chimica acta 2021-04, Vol.1154, p.338306-338306, Article 338306
Hauptverfasser:	Cao, Cuiyan, Yu, Long, Zhang, Xiuli, Dong, Xuefang, Yuan, Jingli, Liang, Xinmiao
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - metabolism Calibrated quantitation Calibration Fc glycosylation Fc-glycopeptide standards Glycopeptides Glycosylation Immunoglobulin G Monoclonal antibodies Negative ESI-MSMS Two-dimensional purification
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