Differential response induced by LPS and MPLA in immunocompetent and septic individuals
Lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA) induce, overall, similar transcriptional profiles in healthy individuals, although LPS has been shown to more potently induce pro-inflammatory cytokines. We explore herein whether MPLA could be considered as a synthetic replacement of LPS in...
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| Veröffentlicht in: | Clinical immunology (Orlando, Fla.) Fla.), 2021-05, Vol.226, p.108714-108714, Article 108714 |
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| container_title | Clinical immunology (Orlando, Fla.) |
| container_volume | 226 |
| creator | Albert Vega, Chloé Karakike, Eleni Bartolo, François Mouton, William Cerrato, Elisabeth Brengel-Pesce, Karen Giamarellos-Bourboulis, Evangelos J. Mallet, François Trouillet-Assant, Sophie |
| description | Lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA) induce, overall, similar transcriptional profiles in healthy individuals, although LPS has been shown to more potently induce pro-inflammatory cytokines. We explore herein whether MPLA could be considered as a synthetic replacement of LPS in immune functional assays to study anergy of immune cells in septic patients. Ex vivo whole blood stimulation with MPLA revealed a lower induction of the TNFα secreted protein in 20 septic patients (SP) compared to 10 healthy volunteers (HV), in agreement with monocyte anergy. Principal component analysis of the 93-gene molecular response to MPLA and LPS stimulation found that the main variability was driven by stimulation in HV and by pathophysiology in SP. MPLA was a stronger inducer of the HLA family genes than LPS in both populations, arguing for divergent signalling pathways downstream of TLR-4. In addition, MPLA appeared to present a more informative stratification potential within the septic population. |
| doi_str_mv | 10.1016/j.clim.2021.108714 |
| format | Article |
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| ispartof | Clinical immunology (Orlando, Fla.), 2021-05, Vol.226, p.108714-108714, Article 108714 |
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| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Immune functional assay LPS MPLA Sepsis Septic patients Stratification |
| title | Differential response induced by LPS and MPLA in immunocompetent and septic individuals |
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