Sca1+ Progenitor Cells (Ex vivo) Exhibits Differential Proteomic Signatures From the Culture Adapted Sca1+ Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue
Stem cell antigen-1 (Sca-1) is a glycosyl-phosphatidylinositol-anchored membrane protein that is expressed in a sub-population of muscle stem and progenitor cell types. Reportedly, Sca-1 regulates the myogenic property of myoblasts and Sca-1 −/− mice exhibited defective muscle regeneration. Although...
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| Veröffentlicht in: | Stem cell reviews and reports 2021-10, Vol.17 (5), p.1754-1767 |
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| creator | Kapoor, Saketh Subba, Pratigya Shenoy P, Sudheer Bose, Bipasha |
| description | Stem cell antigen-1 (Sca-1) is a glycosyl-phosphatidylinositol-anchored membrane protein that is expressed in a sub-population of muscle stem and progenitor cell types. Reportedly, Sca-1 regulates the myogenic property of myoblasts and
Sca-1
−/−
mice exhibited defective muscle regeneration. Although the role of Sca-1 in muscle development and maintenance is well-acknowledged, molecular composition of muscle derived Sca-1
+
cells is not characterized. Here, we applied a high-resolution mass spectrometry-based workflow to characterize the proteomic landscape of mouse hindlimb skeletal muscle derived Sca-1
+
cells. Furthermore, we characterized the impact of the cellular microenvironments on the proteomes of Sca-1
+
cells. The proteome component of freshly isolated Sca-1
+
cells (
ex vivo
) was compared with that of Sca-1
+
cells expanded in cell culture (
in vitro
). The analysis revealed significant differences in the protein abundances in the two conditions reflective of their functional variations. The identified proteins were enriched in various biological pathways. Notably, we identified proteins related to myotube differentiation, myotube cell development and myoblast fusion. We also identified a panel of cell surface marker proteins that can be leveraged in future to enrich Sca-1
+
cells using combinatorial strategies. Comparative analysis implicated the activation of various pathways leading to increased protein synthesis under
in vitro
condition. We report here the most comprehensive proteome map of Sca-1
+
cells that provides insights into the molecular networks operative in Sca-1
+
cells. Importantly, through our work we generated the proteomic blueprint of protein abundances significantly altered in Sca-1
+
cells under
ex vivo
and
in vitro
conditions. The curated data can also be visualized at
https://yenepoya.res.in/database/Sca-1-Proteomics
.
Graphical Abstract |
| doi_str_mv | 10.1007/s12015-021-10134-w |
| format | Article |
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Sca-1
−/−
mice exhibited defective muscle regeneration. Although the role of Sca-1 in muscle development and maintenance is well-acknowledged, molecular composition of muscle derived Sca-1
+
cells is not characterized. Here, we applied a high-resolution mass spectrometry-based workflow to characterize the proteomic landscape of mouse hindlimb skeletal muscle derived Sca-1
+
cells. Furthermore, we characterized the impact of the cellular microenvironments on the proteomes of Sca-1
+
cells. The proteome component of freshly isolated Sca-1
+
cells (
ex vivo
) was compared with that of Sca-1
+
cells expanded in cell culture (
in vitro
). The analysis revealed significant differences in the protein abundances in the two conditions reflective of their functional variations. The identified proteins were enriched in various biological pathways. Notably, we identified proteins related to myotube differentiation, myotube cell development and myoblast fusion. We also identified a panel of cell surface marker proteins that can be leveraged in future to enrich Sca-1
+
cells using combinatorial strategies. Comparative analysis implicated the activation of various pathways leading to increased protein synthesis under
in vitro
condition. We report here the most comprehensive proteome map of Sca-1
+
cells that provides insights into the molecular networks operative in Sca-1
+
cells. Importantly, through our work we generated the proteomic blueprint of protein abundances significantly altered in Sca-1
+
cells under
ex vivo
and
in vitro
conditions. The curated data can also be visualized at
https://yenepoya.res.in/database/Sca-1-Proteomics
.
Graphical Abstract</description><identifier>ISSN: 2629-3269</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-021-10134-w</identifier><identifier>PMID: 33742350</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Ataxin ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Cell Biology ; Cell culture ; Cell differentiation ; Cell fusion ; Cell surface ; Comparative analysis ; Life Sciences ; Mass spectroscopy ; Membrane proteins ; Mice ; Microenvironments ; Muscle, Skeletal ; Musculoskeletal system ; Myoblasts ; Myotubes ; Phosphatidylinositol ; Progenitor cells ; Protein biosynthesis ; Proteins ; Proteome ; Proteomes ; Proteomics ; Regenerative Medicine/Tissue Engineering ; Skeletal muscle ; Stem Cells ; Surface markers</subject><ispartof>Stem cell reviews and reports, 2021-10, Vol.17 (5), p.1754-1767</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c370t-4771a3b3b7283fccff82f3a3494a463d08d4e485701ed6e8c17664b1bf4709da3</cites><orcidid>0000-0002-2312-9734</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12015-021-10134-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12015-021-10134-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33742350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kapoor, Saketh</creatorcontrib><creatorcontrib>Subba, Pratigya</creatorcontrib><creatorcontrib>Shenoy P, Sudheer</creatorcontrib><creatorcontrib>Bose, Bipasha</creatorcontrib><title>Sca1+ Progenitor Cells (Ex vivo) Exhibits Differential Proteomic Signatures From the Culture Adapted Sca1+ Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue</title><title>Stem cell reviews and reports</title><addtitle>Stem Cell Rev and Rep</addtitle><addtitle>Stem Cell Rev Rep</addtitle><description>Stem cell antigen-1 (Sca-1) is a glycosyl-phosphatidylinositol-anchored membrane protein that is expressed in a sub-population of muscle stem and progenitor cell types. Reportedly, Sca-1 regulates the myogenic property of myoblasts and
Sca-1
−/−
mice exhibited defective muscle regeneration. Although the role of Sca-1 in muscle development and maintenance is well-acknowledged, molecular composition of muscle derived Sca-1
+
cells is not characterized. Here, we applied a high-resolution mass spectrometry-based workflow to characterize the proteomic landscape of mouse hindlimb skeletal muscle derived Sca-1
+
cells. Furthermore, we characterized the impact of the cellular microenvironments on the proteomes of Sca-1
+
cells. The proteome component of freshly isolated Sca-1
+
cells (
ex vivo
) was compared with that of Sca-1
+
cells expanded in cell culture (
in vitro
). The analysis revealed significant differences in the protein abundances in the two conditions reflective of their functional variations. The identified proteins were enriched in various biological pathways. Notably, we identified proteins related to myotube differentiation, myotube cell development and myoblast fusion. We also identified a panel of cell surface marker proteins that can be leveraged in future to enrich Sca-1
+
cells using combinatorial strategies. Comparative analysis implicated the activation of various pathways leading to increased protein synthesis under
in vitro
condition. We report here the most comprehensive proteome map of Sca-1
+
cells that provides insights into the molecular networks operative in Sca-1
+
cells. Importantly, through our work we generated the proteomic blueprint of protein abundances significantly altered in Sca-1
+
cells under
ex vivo
and
in vitro
conditions. The curated data can also be visualized at
https://yenepoya.res.in/database/Sca-1-Proteomics
.
Graphical Abstract</description><subject>Animals</subject><subject>Ataxin</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell fusion</subject><subject>Cell surface</subject><subject>Comparative analysis</subject><subject>Life Sciences</subject><subject>Mass spectroscopy</subject><subject>Membrane proteins</subject><subject>Mice</subject><subject>Microenvironments</subject><subject>Muscle, Skeletal</subject><subject>Musculoskeletal system</subject><subject>Myoblasts</subject><subject>Myotubes</subject><subject>Phosphatidylinositol</subject><subject>Progenitor cells</subject><subject>Protein biosynthesis</subject><subject>Proteins</subject><subject>Proteome</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Skeletal muscle</subject><subject>Stem Cells</subject><subject>Surface markers</subject><issn>2629-3269</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtv1DAUhS0EolXpH2CBLLFpBQG_Jk6WZZjCSK1AmrK2nOR6xiWJBz_6-FH9j3iaoUgsWF3r-jvnXOkg9JqSD5QQ-TFQRuisIIwWlFAuittn6JCVrC44k_L507usD9BxCNeEEMaJyJqX6IBzKRifkUP0sGo1fYe_e7eG0Ubn8Rz6PuCTxR2-sTfuFC_uNraxMeDP1hjwMEar-50gghtsi1d2PeqYPAR87t2A4wbwPPW7DT7r9DZCh6eQvfNyzM7Ru9P3-JOLG7wMrtc76lF-mbwdAa9-Qg8xB12m0PaAr2wICV6hF0b3AY738wj9OF9czb8WF9--LOdnF0XLJYmFkJJq3vBGsoqbtjWmYoZrLmqhRck7UnUCRDWThEJXQtVSWZaioY0RktSd5kfoZPLdevcrQYhqsKHN5-sRXAqKzQgXQpaCZ_TtP-i1S37M12WqkqISrKozxSaq9S4ED0ZtvR20v1eUqF2faupT5T7VY5_qNove7K1TM0D3JPnTXgb4BIT8Na7B_83-j-1vF8iqyQ</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Kapoor, Saketh</creator><creator>Subba, Pratigya</creator><creator>Shenoy P, Sudheer</creator><creator>Bose, Bipasha</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2312-9734</orcidid></search><sort><creationdate>20211001</creationdate><title>Sca1+ Progenitor Cells (Ex vivo) Exhibits Differential Proteomic Signatures From the Culture Adapted Sca1+ Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue</title><author>Kapoor, Saketh ; Subba, Pratigya ; Shenoy P, Sudheer ; Bose, Bipasha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-4771a3b3b7283fccff82f3a3494a463d08d4e485701ed6e8c17664b1bf4709da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Ataxin</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell fusion</topic><topic>Cell surface</topic><topic>Comparative analysis</topic><topic>Life Sciences</topic><topic>Mass spectroscopy</topic><topic>Membrane proteins</topic><topic>Mice</topic><topic>Microenvironments</topic><topic>Muscle, Skeletal</topic><topic>Musculoskeletal system</topic><topic>Myoblasts</topic><topic>Myotubes</topic><topic>Phosphatidylinositol</topic><topic>Progenitor cells</topic><topic>Protein biosynthesis</topic><topic>Proteins</topic><topic>Proteome</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Skeletal muscle</topic><topic>Stem Cells</topic><topic>Surface markers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kapoor, Saketh</creatorcontrib><creatorcontrib>Subba, Pratigya</creatorcontrib><creatorcontrib>Shenoy P, Sudheer</creatorcontrib><creatorcontrib>Bose, Bipasha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cell reviews and reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kapoor, Saketh</au><au>Subba, Pratigya</au><au>Shenoy P, Sudheer</au><au>Bose, Bipasha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sca1+ Progenitor Cells (Ex vivo) Exhibits Differential Proteomic Signatures From the Culture Adapted Sca1+ Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue</atitle><jtitle>Stem cell reviews and reports</jtitle><stitle>Stem Cell Rev and Rep</stitle><addtitle>Stem Cell Rev Rep</addtitle><date>2021-10-01</date><risdate>2021</risdate><volume>17</volume><issue>5</issue><spage>1754</spage><epage>1767</epage><pages>1754-1767</pages><issn>2629-3269</issn><eissn>2629-3277</eissn><abstract>Stem cell antigen-1 (Sca-1) is a glycosyl-phosphatidylinositol-anchored membrane protein that is expressed in a sub-population of muscle stem and progenitor cell types. Reportedly, Sca-1 regulates the myogenic property of myoblasts and
Sca-1
−/−
mice exhibited defective muscle regeneration. Although the role of Sca-1 in muscle development and maintenance is well-acknowledged, molecular composition of muscle derived Sca-1
+
cells is not characterized. Here, we applied a high-resolution mass spectrometry-based workflow to characterize the proteomic landscape of mouse hindlimb skeletal muscle derived Sca-1
+
cells. Furthermore, we characterized the impact of the cellular microenvironments on the proteomes of Sca-1
+
cells. The proteome component of freshly isolated Sca-1
+
cells (
ex vivo
) was compared with that of Sca-1
+
cells expanded in cell culture (
in vitro
). The analysis revealed significant differences in the protein abundances in the two conditions reflective of their functional variations. The identified proteins were enriched in various biological pathways. Notably, we identified proteins related to myotube differentiation, myotube cell development and myoblast fusion. We also identified a panel of cell surface marker proteins that can be leveraged in future to enrich Sca-1
+
cells using combinatorial strategies. Comparative analysis implicated the activation of various pathways leading to increased protein synthesis under
in vitro
condition. We report here the most comprehensive proteome map of Sca-1
+
cells that provides insights into the molecular networks operative in Sca-1
+
cells. Importantly, through our work we generated the proteomic blueprint of protein abundances significantly altered in Sca-1
+
cells under
ex vivo
and
in vitro
conditions. The curated data can also be visualized at
https://yenepoya.res.in/database/Sca-1-Proteomics
.
Graphical Abstract</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33742350</pmid><doi>10.1007/s12015-021-10134-w</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-2312-9734</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2629-3269 |
| ispartof | Stem cell reviews and reports, 2021-10, Vol.17 (5), p.1754-1767 |
| issn | 2629-3269 2629-3277 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Ataxin Biomedical and Life Sciences Biomedical Engineering and Bioengineering Cell Biology Cell culture Cell differentiation Cell fusion Cell surface Comparative analysis Life Sciences Mass spectroscopy Membrane proteins Mice Microenvironments Muscle, Skeletal Musculoskeletal system Myoblasts Myotubes Phosphatidylinositol Progenitor cells Protein biosynthesis Proteins Proteome Proteomes Proteomics Regenerative Medicine/Tissue Engineering Skeletal muscle Stem Cells Surface markers |
| title | Sca1+ Progenitor Cells (Ex vivo) Exhibits Differential Proteomic Signatures From the Culture Adapted Sca1+ Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue |
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