Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1
Pseudomonas aeruginosa is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the P. aeruginosa pathogenicity. Hence, the targetin...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2021-04, Vol.37 (4), p.66-66, Article 66 |
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| creator | Karuppiah, Vijayakumar Thirunanasambandham, Ramanathan Thangaraj, Gunasekaran |
| description | Pseudomonas aeruginosa
is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the
P. aeruginosa
pathogenicity. Hence, the targeting of QS considered as an alternative approach to control
P. aeruginosa
infections. This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential of
Musa paradisiaca
against
Chromobacterium violaceum
(ATCC 12472) and
Pseudomonas aeruginosa
. The methanol extract of
M. paradisiacsa
exhibits that better antibiofilm potential against
P. aeruginosa
. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract of
M. paradisiaca
revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production in
C. violaceum
. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation of
P. aeruginosa
without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite of
M. paradisiaca
impedes
P. aeruginosa
QS system and associated virulence productions. |
| doi_str_mv | 10.1007/s11274-021-03029-y |
| format | Article |
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is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the
P. aeruginosa
pathogenicity. Hence, the targeting of QS considered as an alternative approach to control
P. aeruginosa
infections. This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential of
Musa paradisiaca
against
Chromobacterium violaceum
(ATCC 12472) and
Pseudomonas aeruginosa
. The methanol extract of
M. paradisiacsa
exhibits that better antibiofilm potential against
P. aeruginosa
. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract of
M. paradisiaca
revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production in
C. violaceum
. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation of
P. aeruginosa
without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite of
M. paradisiaca
impedes
P. aeruginosa
QS system and associated virulence productions.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-021-03029-y</identifier><identifier>PMID: 33740144</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Applied Microbiology ; Biochemistry ; Biofilms ; Biomedical and Life Sciences ; Biotechnology ; Cineole ; Column chromatography ; Cystic fibrosis ; Environmental Engineering/Biotechnology ; Life Sciences ; Metabolites ; Methanol ; Microbiology ; Musa paradisiaca ; Nosocomial infection ; Nosocomial infections ; Opportunist infection ; Original Paper ; Pathogenicity ; Pathogens ; Pseudomonas aeruginosa ; Quorum sensing ; Spectrometry ; Surgical site infections ; Transcription ; Violacein ; Virulence</subject><ispartof>World journal of microbiology & biotechnology, 2021-04, Vol.37 (4), p.66-66, Article 66</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-ae7baccc773be405c4b458a4db3be1750d66f0f7757cfb1c9196fcab0a3eaaf3</citedby><cites>FETCH-LOGICAL-c412t-ae7baccc773be405c4b458a4db3be1750d66f0f7757cfb1c9196fcab0a3eaaf3</cites><orcidid>0000-0002-7651-760X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11274-021-03029-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11274-021-03029-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33740144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karuppiah, Vijayakumar</creatorcontrib><creatorcontrib>Thirunanasambandham, Ramanathan</creatorcontrib><creatorcontrib>Thangaraj, Gunasekaran</creatorcontrib><title>Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>Pseudomonas aeruginosa
is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the
P. aeruginosa
pathogenicity. Hence, the targeting of QS considered as an alternative approach to control
P. aeruginosa
infections. This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential of
Musa paradisiaca
against
Chromobacterium violaceum
(ATCC 12472) and
Pseudomonas aeruginosa
. The methanol extract of
M. paradisiacsa
exhibits that better antibiofilm potential against
P. aeruginosa
. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract of
M. paradisiaca
revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production in
C. violaceum
. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation of
P. aeruginosa
without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite of
M. paradisiaca
impedes
P. aeruginosa
QS system and associated virulence productions.</description><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cineole</subject><subject>Column chromatography</subject><subject>Cystic fibrosis</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Life Sciences</subject><subject>Metabolites</subject><subject>Methanol</subject><subject>Microbiology</subject><subject>Musa paradisiaca</subject><subject>Nosocomial infection</subject><subject>Nosocomial infections</subject><subject>Opportunist infection</subject><subject>Original Paper</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Pseudomonas aeruginosa</subject><subject>Quorum sensing</subject><subject>Spectrometry</subject><subject>Surgical site 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Biotechnol</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>37</volume><issue>4</issue><spage>66</spage><epage>66</epage><pages>66-66</pages><artnum>66</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Pseudomonas aeruginosa
is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the
P. aeruginosa
pathogenicity. Hence, the targeting of QS considered as an alternative approach to control
P. aeruginosa
infections. This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential of
Musa paradisiaca
against
Chromobacterium violaceum
(ATCC 12472) and
Pseudomonas aeruginosa
. The methanol extract of
M. paradisiacsa
exhibits that better antibiofilm potential against
P. aeruginosa
. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract of
M. paradisiaca
revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production in
C. violaceum
. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation of
P. aeruginosa
without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite of
M. paradisiaca
impedes
P. aeruginosa
QS system and associated virulence productions.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>33740144</pmid><doi>10.1007/s11274-021-03029-y</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-7651-760X</orcidid></addata></record> |
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| issn | 0959-3993 1573-0972 |
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| source | SpringerNature Journals |
| subjects | Applied Microbiology Biochemistry Biofilms Biomedical and Life Sciences Biotechnology Cineole Column chromatography Cystic fibrosis Environmental Engineering/Biotechnology Life Sciences Metabolites Methanol Microbiology Musa paradisiaca Nosocomial infection Nosocomial infections Opportunist infection Original Paper Pathogenicity Pathogens Pseudomonas aeruginosa Quorum sensing Spectrometry Surgical site infections Transcription Violacein Virulence |
| title | Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1 |
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