Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals

Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as the jaguar. Cryobanks of skins have the potential to safeguard rare genotypes, allowing the potential exploitation of biological samples in animal multiplication technologies and the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Zoo biology 2021-07, Vol.40 (4), p.280-287
Hauptverfasser: Santos, Maria D. C. B., Aquino, Leonardo V. C., Nascimento, Matheus B., Silva, Maria B., Rodrigues, Luanna L. V., Praxedes, Érika A., Oliveira, Lhara R. M., Silva, Herlon V. R., Nunes, Thalles G. P., Oliveira, Moacir F., Pereira, Alexsandra F.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 287
container_issue 4
container_start_page 280
container_title Zoo biology
container_volume 40
creator Santos, Maria D. C. B.
Aquino, Leonardo V. C.
Nascimento, Matheus B.
Silva, Maria B.
Rodrigues, Luanna L. V.
Praxedes, Érika A.
Oliveira, Lhara R. M.
Silva, Herlon V. R.
Nunes, Thalles G. P.
Oliveira, Moacir F.
Pereira, Alexsandra F.
description Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as the jaguar. Cryobanks of skins have the potential to safeguard rare genotypes, allowing the potential exploitation of biological samples in animal multiplication technologies and the study of genetic variability. Determination of the most suitable skin regions for tissue conservation can help increase the efficiency of cryobanks and the storage of biological samples. To this end, we evaluated the effects of vitrification of skin tissues from the ear, caudal, and femoral regions of a post‐mortem jaguar belonging to a zoo in Brazil. Non‐vitrified and vitrified samples were evaluated and compared using quantitative methods, focusing on skin thickness, cell quantification, number of perinuclear halos, collagen and elastic density, and proliferative activity. No differences were observed in skin thickness, number of perinuclear halos, elastic density, and proliferative activity between non‐vitrified and vitrified tissues in skin from any region. However, vitrified tissues derived from femoral skin showed a reduction in the number of fibroblasts, epidermal cells and collagen density compared to non‐vitrified tissues. In summary, the ear and caudal regions provided the best conservation of somatic tissues derived from jaguars, and skin samples from these regions are therefore the most suitable for the formation of cryobanks. Highlights Ear and caudal regions were the most suitable for the conservation of somatic tissues derived from jaguars. A greater number of fibroblasts was found in ear skin compared to other skin regions. These results are important to contribute to jaguar's conservation.
doi_str_mv 10.1002/zoo.21605
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2503435945</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2503435945</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-1b1b234e08425266189fee2809625bfb5756a5a39cd592c57f9c877259266e913</originalsourceid><addsrcrecordid>eNp1kc1u1DAURi0EotPCghdAlti00qT1Txw7S1S1gDTSdAEbNpHjXBdPEzvYyaDpM_UhcUlhgdTVlXWPznetD6F3lJxTQtjFfQjnjFZEvEArSmpVMF7Rl2hFJOcFp0odoeOUdoQQJSh7jY44l7wuK7lCD1d73c96csHjYHHnrIUIfsLpznkc4TYvEu4guj102MYwYI3HkKYhxAkGvNO3s45rfKP99AOixsEbjU83znsNc1pjKoU6W2NtJ4h476borDNLng0xm_fQh3F4jMz5Jh5Cq_1dWqKMHqccjLV3g-7TG_TK5gFvn-YJ-nZ99fXyc7HZfvpy-XFTGC64KGhLW8ZLIKpkglUVVbUFYIrUFROtbYUUlRaa16YTNTNC2tooKVl-VBXUlJ-g08U7xvBzhjQ1g0sG-l57CHNqmCC85KIuRUY__Ifuwhx9vi5TQlLBiSKZOlsoE0NKEWwzxvyjeGgoaR4rbHKFzZ8KM_v-yTi3A3T_yL-dZeBiAX65Hg7Pm5rv2-2i_A3hI6ZK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2557153080</pqid></control><display><type>article</type><title>Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals</title><source>Access via Wiley Online Library</source><creator>Santos, Maria D. C. B. ; Aquino, Leonardo V. C. ; Nascimento, Matheus B. ; Silva, Maria B. ; Rodrigues, Luanna L. V. ; Praxedes, Érika A. ; Oliveira, Lhara R. M. ; Silva, Herlon V. R. ; Nunes, Thalles G. P. ; Oliveira, Moacir F. ; Pereira, Alexsandra F.</creator><creatorcontrib>Santos, Maria D. C. B. ; Aquino, Leonardo V. C. ; Nascimento, Matheus B. ; Silva, Maria B. ; Rodrigues, Luanna L. V. ; Praxedes, Érika A. ; Oliveira, Lhara R. M. ; Silva, Herlon V. R. ; Nunes, Thalles G. P. ; Oliveira, Moacir F. ; Pereira, Alexsandra F.</creatorcontrib><description>Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as the jaguar. Cryobanks of skins have the potential to safeguard rare genotypes, allowing the potential exploitation of biological samples in animal multiplication technologies and the study of genetic variability. Determination of the most suitable skin regions for tissue conservation can help increase the efficiency of cryobanks and the storage of biological samples. To this end, we evaluated the effects of vitrification of skin tissues from the ear, caudal, and femoral regions of a post‐mortem jaguar belonging to a zoo in Brazil. Non‐vitrified and vitrified samples were evaluated and compared using quantitative methods, focusing on skin thickness, cell quantification, number of perinuclear halos, collagen and elastic density, and proliferative activity. No differences were observed in skin thickness, number of perinuclear halos, elastic density, and proliferative activity between non‐vitrified and vitrified tissues in skin from any region. However, vitrified tissues derived from femoral skin showed a reduction in the number of fibroblasts, epidermal cells and collagen density compared to non‐vitrified tissues. In summary, the ear and caudal regions provided the best conservation of somatic tissues derived from jaguars, and skin samples from these regions are therefore the most suitable for the formation of cryobanks. Highlights Ear and caudal regions were the most suitable for the conservation of somatic tissues derived from jaguars. A greater number of fibroblasts was found in ear skin compared to other skin regions. These results are important to contribute to jaguar's conservation.</description><identifier>ISSN: 0733-3188</identifier><identifier>EISSN: 1098-2361</identifier><identifier>DOI: 10.1002/zoo.21605</identifier><identifier>PMID: 33739467</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>big cats ; Biological properties ; biological resource banks ; Biological samples ; Collagen ; Conservation ; Density ; Ear ; Femur ; Fibroblasts ; Genetic variability ; Genotypes ; Halos ; Multiplication ; Necropsy ; Panthera onca ; Skin ; Species extinction ; Thickness ; Threatened species ; tissue cryopreservation ; Tissues ; Vitrification ; Wildlife conservation ; Zoo animals</subject><ispartof>Zoo biology, 2021-07, Vol.40 (4), p.280-287</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-1b1b234e08425266189fee2809625bfb5756a5a39cd592c57f9c877259266e913</citedby><cites>FETCH-LOGICAL-c3535-1b1b234e08425266189fee2809625bfb5756a5a39cd592c57f9c877259266e913</cites><orcidid>0000-0003-2137-854X ; 0000-0001-7192-7581</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fzoo.21605$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fzoo.21605$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33739467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santos, Maria D. C. B.</creatorcontrib><creatorcontrib>Aquino, Leonardo V. C.</creatorcontrib><creatorcontrib>Nascimento, Matheus B.</creatorcontrib><creatorcontrib>Silva, Maria B.</creatorcontrib><creatorcontrib>Rodrigues, Luanna L. V.</creatorcontrib><creatorcontrib>Praxedes, Érika A.</creatorcontrib><creatorcontrib>Oliveira, Lhara R. M.</creatorcontrib><creatorcontrib>Silva, Herlon V. R.</creatorcontrib><creatorcontrib>Nunes, Thalles G. P.</creatorcontrib><creatorcontrib>Oliveira, Moacir F.</creatorcontrib><creatorcontrib>Pereira, Alexsandra F.</creatorcontrib><title>Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals</title><title>Zoo biology</title><addtitle>Zoo Biol</addtitle><description>Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as the jaguar. Cryobanks of skins have the potential to safeguard rare genotypes, allowing the potential exploitation of biological samples in animal multiplication technologies and the study of genetic variability. Determination of the most suitable skin regions for tissue conservation can help increase the efficiency of cryobanks and the storage of biological samples. To this end, we evaluated the effects of vitrification of skin tissues from the ear, caudal, and femoral regions of a post‐mortem jaguar belonging to a zoo in Brazil. Non‐vitrified and vitrified samples were evaluated and compared using quantitative methods, focusing on skin thickness, cell quantification, number of perinuclear halos, collagen and elastic density, and proliferative activity. No differences were observed in skin thickness, number of perinuclear halos, elastic density, and proliferative activity between non‐vitrified and vitrified tissues in skin from any region. However, vitrified tissues derived from femoral skin showed a reduction in the number of fibroblasts, epidermal cells and collagen density compared to non‐vitrified tissues. In summary, the ear and caudal regions provided the best conservation of somatic tissues derived from jaguars, and skin samples from these regions are therefore the most suitable for the formation of cryobanks. Highlights Ear and caudal regions were the most suitable for the conservation of somatic tissues derived from jaguars. A greater number of fibroblasts was found in ear skin compared to other skin regions. These results are important to contribute to jaguar's conservation.</description><subject>big cats</subject><subject>Biological properties</subject><subject>biological resource banks</subject><subject>Biological samples</subject><subject>Collagen</subject><subject>Conservation</subject><subject>Density</subject><subject>Ear</subject><subject>Femur</subject><subject>Fibroblasts</subject><subject>Genetic variability</subject><subject>Genotypes</subject><subject>Halos</subject><subject>Multiplication</subject><subject>Necropsy</subject><subject>Panthera onca</subject><subject>Skin</subject><subject>Species extinction</subject><subject>Thickness</subject><subject>Threatened species</subject><subject>tissue cryopreservation</subject><subject>Tissues</subject><subject>Vitrification</subject><subject>Wildlife conservation</subject><subject>Zoo animals</subject><issn>0733-3188</issn><issn>1098-2361</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1DAURi0EotPCghdAlti00qT1Txw7S1S1gDTSdAEbNpHjXBdPEzvYyaDpM_UhcUlhgdTVlXWPznetD6F3lJxTQtjFfQjnjFZEvEArSmpVMF7Rl2hFJOcFp0odoeOUdoQQJSh7jY44l7wuK7lCD1d73c96csHjYHHnrIUIfsLpznkc4TYvEu4guj102MYwYI3HkKYhxAkGvNO3s45rfKP99AOixsEbjU83znsNc1pjKoU6W2NtJ4h476borDNLng0xm_fQh3F4jMz5Jh5Cq_1dWqKMHqccjLV3g-7TG_TK5gFvn-YJ-nZ99fXyc7HZfvpy-XFTGC64KGhLW8ZLIKpkglUVVbUFYIrUFROtbYUUlRaa16YTNTNC2tooKVl-VBXUlJ-g08U7xvBzhjQ1g0sG-l57CHNqmCC85KIuRUY__Ifuwhx9vi5TQlLBiSKZOlsoE0NKEWwzxvyjeGgoaR4rbHKFzZ8KM_v-yTi3A3T_yL-dZeBiAX65Hg7Pm5rv2-2i_A3hI6ZK</recordid><startdate>202107</startdate><enddate>202107</enddate><creator>Santos, Maria D. C. B.</creator><creator>Aquino, Leonardo V. C.</creator><creator>Nascimento, Matheus B.</creator><creator>Silva, Maria B.</creator><creator>Rodrigues, Luanna L. V.</creator><creator>Praxedes, Érika A.</creator><creator>Oliveira, Lhara R. M.</creator><creator>Silva, Herlon V. R.</creator><creator>Nunes, Thalles G. P.</creator><creator>Oliveira, Moacir F.</creator><creator>Pereira, Alexsandra F.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>C1K</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2137-854X</orcidid><orcidid>https://orcid.org/0000-0001-7192-7581</orcidid></search><sort><creationdate>202107</creationdate><title>Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals</title><author>Santos, Maria D. C. B. ; Aquino, Leonardo V. C. ; Nascimento, Matheus B. ; Silva, Maria B. ; Rodrigues, Luanna L. V. ; Praxedes, Érika A. ; Oliveira, Lhara R. M. ; Silva, Herlon V. R. ; Nunes, Thalles G. P. ; Oliveira, Moacir F. ; Pereira, Alexsandra F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-1b1b234e08425266189fee2809625bfb5756a5a39cd592c57f9c877259266e913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>big cats</topic><topic>Biological properties</topic><topic>biological resource banks</topic><topic>Biological samples</topic><topic>Collagen</topic><topic>Conservation</topic><topic>Density</topic><topic>Ear</topic><topic>Femur</topic><topic>Fibroblasts</topic><topic>Genetic variability</topic><topic>Genotypes</topic><topic>Halos</topic><topic>Multiplication</topic><topic>Necropsy</topic><topic>Panthera onca</topic><topic>Skin</topic><topic>Species extinction</topic><topic>Thickness</topic><topic>Threatened species</topic><topic>tissue cryopreservation</topic><topic>Tissues</topic><topic>Vitrification</topic><topic>Wildlife conservation</topic><topic>Zoo animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos, Maria D. C. B.</creatorcontrib><creatorcontrib>Aquino, Leonardo V. C.</creatorcontrib><creatorcontrib>Nascimento, Matheus B.</creatorcontrib><creatorcontrib>Silva, Maria B.</creatorcontrib><creatorcontrib>Rodrigues, Luanna L. V.</creatorcontrib><creatorcontrib>Praxedes, Érika A.</creatorcontrib><creatorcontrib>Oliveira, Lhara R. M.</creatorcontrib><creatorcontrib>Silva, Herlon V. R.</creatorcontrib><creatorcontrib>Nunes, Thalles G. P.</creatorcontrib><creatorcontrib>Oliveira, Moacir F.</creatorcontrib><creatorcontrib>Pereira, Alexsandra F.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Zoo biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos, Maria D. C. B.</au><au>Aquino, Leonardo V. C.</au><au>Nascimento, Matheus B.</au><au>Silva, Maria B.</au><au>Rodrigues, Luanna L. V.</au><au>Praxedes, Érika A.</au><au>Oliveira, Lhara R. M.</au><au>Silva, Herlon V. R.</au><au>Nunes, Thalles G. P.</au><au>Oliveira, Moacir F.</au><au>Pereira, Alexsandra F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals</atitle><jtitle>Zoo biology</jtitle><addtitle>Zoo Biol</addtitle><date>2021-07</date><risdate>2021</risdate><volume>40</volume><issue>4</issue><spage>280</spage><epage>287</epage><pages>280-287</pages><issn>0733-3188</issn><eissn>1098-2361</eissn><abstract>Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as the jaguar. Cryobanks of skins have the potential to safeguard rare genotypes, allowing the potential exploitation of biological samples in animal multiplication technologies and the study of genetic variability. Determination of the most suitable skin regions for tissue conservation can help increase the efficiency of cryobanks and the storage of biological samples. To this end, we evaluated the effects of vitrification of skin tissues from the ear, caudal, and femoral regions of a post‐mortem jaguar belonging to a zoo in Brazil. Non‐vitrified and vitrified samples were evaluated and compared using quantitative methods, focusing on skin thickness, cell quantification, number of perinuclear halos, collagen and elastic density, and proliferative activity. No differences were observed in skin thickness, number of perinuclear halos, elastic density, and proliferative activity between non‐vitrified and vitrified tissues in skin from any region. However, vitrified tissues derived from femoral skin showed a reduction in the number of fibroblasts, epidermal cells and collagen density compared to non‐vitrified tissues. In summary, the ear and caudal regions provided the best conservation of somatic tissues derived from jaguars, and skin samples from these regions are therefore the most suitable for the formation of cryobanks. Highlights Ear and caudal regions were the most suitable for the conservation of somatic tissues derived from jaguars. A greater number of fibroblasts was found in ear skin compared to other skin regions. These results are important to contribute to jaguar's conservation.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33739467</pmid><doi>10.1002/zoo.21605</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2137-854X</orcidid><orcidid>https://orcid.org/0000-0001-7192-7581</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0733-3188
ispartof Zoo biology, 2021-07, Vol.40 (4), p.280-287
issn 0733-3188
1098-2361
language eng
recordid cdi_proquest_miscellaneous_2503435945
source Access via Wiley Online Library
subjects big cats
Biological properties
biological resource banks
Biological samples
Collagen
Conservation
Density
Ear
Femur
Fibroblasts
Genetic variability
Genotypes
Halos
Multiplication
Necropsy
Panthera onca
Skin
Species extinction
Thickness
Threatened species
tissue cryopreservation
Tissues
Vitrification
Wildlife conservation
Zoo animals
title Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T19%3A51%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20different%20skin%20regions%20derived%20from%20a%20postmortem%20jaguar,%20Panthera%20onca%20(Linnaeus,%201758),%20after%20vitrification%20for%20development%20of%20cryobanks%20from%20captive%20animals&rft.jtitle=Zoo%20biology&rft.au=Santos,%20Maria%20D.%20C.%20B.&rft.date=2021-07&rft.volume=40&rft.issue=4&rft.spage=280&rft.epage=287&rft.pages=280-287&rft.issn=0733-3188&rft.eissn=1098-2361&rft_id=info:doi/10.1002/zoo.21605&rft_dat=%3Cproquest_cross%3E2503435945%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2557153080&rft_id=info:pmid/33739467&rfr_iscdi=true