Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn
Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a prospect...
Gespeichert in:
Veröffentlicht in: | Acta Physiologica 2021-07, Vol.232 (3), p.e13647-e13647 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | e13647 |
---|---|
container_issue | 3 |
container_start_page | e13647 |
container_title | Acta Physiologica |
container_volume | 232 |
creator | Klein, Marie Kaestner, Lars Bogdanova, Anna Y Minetti, Giampaolo Rudloff, Silvia Lundby, Carsten Makhro, Asya Seiler, Elena van Cromvoirt, Ankie Fenk, Simone Simionato, Greta Hertz, Laura Recktenwald, Steffen Schäfer, Larissa Haider, Thomas Fried, Sebastian Borsch, Christian Marti, Hugo H Sander, Anja Mairbäurl, Heimo |
description | Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a prospective study on erythrocyte survival after a stay at the Jungfraujoch Research Station (JFJRS; 3450 m).
Newly formed erythrocytes of 12 male subjects (mean age 23.3 years) were age cohort labelled in normoxia (110 m) and during a 19-day high-altitude sojourn by ingestion of
C2- and
N-labelled glycine respectively. Elimination dynamics for erythrocytes produced in normoxia and at high altitude were measured by isotope ratio mass spectrometry of haem, by determining tot-Hb, reticulocyte counts, erythrocyte membrane protein 4.1a/4.1b ratio and by mathematical modelling.
Tot-Hb increased by 4.7% ± 2.7% at high altitude and returned to pre-altitude values within 11 days after descent. Elimination of
C- (normoxia) and
N- (high altitude) labelled erythrocytes was not different. Erythropoietin levels and counts of CD71-positive reticulocytes decreased rapidly after descent. The band 4.1a/4.1b ratio decreased at altitude and remained low for 3-4 days after descent and normalized slowly. There was no indication of haemolysis.
We confirm a rapid normalization of tot-Hb upon descent. Based on the lack of accelerated removal of age cohorts of erythrocytes labelled at high altitude, on patterns of changes in reticulocyte counts and of the band 4.1a/4.1b ratio and on modelling, this decrease did not occur via neocytolysis, but by a reduced rate of erythropoiesis along with normal clearance of senescent erythrocytes. |
doi_str_mv | 10.1111/apha.13647 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2502212982</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2502212982</sourcerecordid><originalsourceid>FETCH-LOGICAL-c323t-f21d36854de96b78e3b94cec85b524b949ab46d2c68850e26a0cbb66c875226e3</originalsourceid><addsrcrecordid>eNo9kF1LwzAUhoMobszd-AMklyJ0Jidtml6O4RcMdqPXJUlP1862mUmL7N_b6dy5ed-LhxfOQ8gtZws-3qPeV3rBhYzTCzLlaawinnJ5ee5MTcg8hB1jjAMXMcA1mQiRQiZTmJLN0gTsLFJX0g6dPfSuOYQ60Lqj1dDqLlCP_eC7utvS0ruWaiqib8RPWtXbKtJNX_dDgTS4nRuxG3JV6ibg_JQz8vH89L56jdabl7fVch1ZAaKPSuCFkCqJC8ykSRUKk8UWrUpMAvHYM21iWYCVSiUMQWpmjZHSqjQBkChm5P5vd-_d14Chz9s6WGwaPX4xhBwSBsAhUzCiD3-o9S4Ej2W-93Wr_SHnLD86zI8O81-HI3x32h1Mi8UZ_TcmfgBdDWw7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2502212982</pqid></control><display><type>article</type><title>Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn</title><source>Access via Wiley Online Library</source><creator>Klein, Marie ; Kaestner, Lars ; Bogdanova, Anna Y ; Minetti, Giampaolo ; Rudloff, Silvia ; Lundby, Carsten ; Makhro, Asya ; Seiler, Elena ; van Cromvoirt, Ankie ; Fenk, Simone ; Simionato, Greta ; Hertz, Laura ; Recktenwald, Steffen ; Schäfer, Larissa ; Haider, Thomas ; Fried, Sebastian ; Borsch, Christian ; Marti, Hugo H ; Sander, Anja ; Mairbäurl, Heimo</creator><creatorcontrib>Klein, Marie ; Kaestner, Lars ; Bogdanova, Anna Y ; Minetti, Giampaolo ; Rudloff, Silvia ; Lundby, Carsten ; Makhro, Asya ; Seiler, Elena ; van Cromvoirt, Ankie ; Fenk, Simone ; Simionato, Greta ; Hertz, Laura ; Recktenwald, Steffen ; Schäfer, Larissa ; Haider, Thomas ; Fried, Sebastian ; Borsch, Christian ; Marti, Hugo H ; Sander, Anja ; Mairbäurl, Heimo</creatorcontrib><description>Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a prospective study on erythrocyte survival after a stay at the Jungfraujoch Research Station (JFJRS; 3450 m).
Newly formed erythrocytes of 12 male subjects (mean age 23.3 years) were age cohort labelled in normoxia (110 m) and during a 19-day high-altitude sojourn by ingestion of
C2- and
N-labelled glycine respectively. Elimination dynamics for erythrocytes produced in normoxia and at high altitude were measured by isotope ratio mass spectrometry of haem, by determining tot-Hb, reticulocyte counts, erythrocyte membrane protein 4.1a/4.1b ratio and by mathematical modelling.
Tot-Hb increased by 4.7% ± 2.7% at high altitude and returned to pre-altitude values within 11 days after descent. Elimination of
C- (normoxia) and
N- (high altitude) labelled erythrocytes was not different. Erythropoietin levels and counts of CD71-positive reticulocytes decreased rapidly after descent. The band 4.1a/4.1b ratio decreased at altitude and remained low for 3-4 days after descent and normalized slowly. There was no indication of haemolysis.
We confirm a rapid normalization of tot-Hb upon descent. Based on the lack of accelerated removal of age cohorts of erythrocytes labelled at high altitude, on patterns of changes in reticulocyte counts and of the band 4.1a/4.1b ratio and on modelling, this decrease did not occur via neocytolysis, but by a reduced rate of erythropoiesis along with normal clearance of senescent erythrocytes.</description><identifier>ISSN: 1748-1708</identifier><identifier>EISSN: 1748-1716</identifier><identifier>DOI: 10.1111/apha.13647</identifier><identifier>PMID: 33729672</identifier><language>eng</language><publisher>England</publisher><ispartof>Acta Physiologica, 2021-07, Vol.232 (3), p.e13647-e13647</ispartof><rights>2021 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-f21d36854de96b78e3b94cec85b524b949ab46d2c68850e26a0cbb66c875226e3</citedby><cites>FETCH-LOGICAL-c323t-f21d36854de96b78e3b94cec85b524b949ab46d2c68850e26a0cbb66c875226e3</cites><orcidid>0000-0002-1684-0026 ; 0000-0001-5064-4515 ; 0000-0003-0741-1948 ; 0000-0001-7785-2648</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33729672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klein, Marie</creatorcontrib><creatorcontrib>Kaestner, Lars</creatorcontrib><creatorcontrib>Bogdanova, Anna Y</creatorcontrib><creatorcontrib>Minetti, Giampaolo</creatorcontrib><creatorcontrib>Rudloff, Silvia</creatorcontrib><creatorcontrib>Lundby, Carsten</creatorcontrib><creatorcontrib>Makhro, Asya</creatorcontrib><creatorcontrib>Seiler, Elena</creatorcontrib><creatorcontrib>van Cromvoirt, Ankie</creatorcontrib><creatorcontrib>Fenk, Simone</creatorcontrib><creatorcontrib>Simionato, Greta</creatorcontrib><creatorcontrib>Hertz, Laura</creatorcontrib><creatorcontrib>Recktenwald, Steffen</creatorcontrib><creatorcontrib>Schäfer, Larissa</creatorcontrib><creatorcontrib>Haider, Thomas</creatorcontrib><creatorcontrib>Fried, Sebastian</creatorcontrib><creatorcontrib>Borsch, Christian</creatorcontrib><creatorcontrib>Marti, Hugo H</creatorcontrib><creatorcontrib>Sander, Anja</creatorcontrib><creatorcontrib>Mairbäurl, Heimo</creatorcontrib><title>Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn</title><title>Acta Physiologica</title><addtitle>Acta Physiol (Oxf)</addtitle><description>Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a prospective study on erythrocyte survival after a stay at the Jungfraujoch Research Station (JFJRS; 3450 m).
Newly formed erythrocytes of 12 male subjects (mean age 23.3 years) were age cohort labelled in normoxia (110 m) and during a 19-day high-altitude sojourn by ingestion of
C2- and
N-labelled glycine respectively. Elimination dynamics for erythrocytes produced in normoxia and at high altitude were measured by isotope ratio mass spectrometry of haem, by determining tot-Hb, reticulocyte counts, erythrocyte membrane protein 4.1a/4.1b ratio and by mathematical modelling.
Tot-Hb increased by 4.7% ± 2.7% at high altitude and returned to pre-altitude values within 11 days after descent. Elimination of
C- (normoxia) and
N- (high altitude) labelled erythrocytes was not different. Erythropoietin levels and counts of CD71-positive reticulocytes decreased rapidly after descent. The band 4.1a/4.1b ratio decreased at altitude and remained low for 3-4 days after descent and normalized slowly. There was no indication of haemolysis.
We confirm a rapid normalization of tot-Hb upon descent. Based on the lack of accelerated removal of age cohorts of erythrocytes labelled at high altitude, on patterns of changes in reticulocyte counts and of the band 4.1a/4.1b ratio and on modelling, this decrease did not occur via neocytolysis, but by a reduced rate of erythropoiesis along with normal clearance of senescent erythrocytes.</description><issn>1748-1708</issn><issn>1748-1716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNo9kF1LwzAUhoMobszd-AMklyJ0Jidtml6O4RcMdqPXJUlP1862mUmL7N_b6dy5ed-LhxfOQ8gtZws-3qPeV3rBhYzTCzLlaawinnJ5ee5MTcg8hB1jjAMXMcA1mQiRQiZTmJLN0gTsLFJX0g6dPfSuOYQ60Lqj1dDqLlCP_eC7utvS0ruWaiqib8RPWtXbKtJNX_dDgTS4nRuxG3JV6ibg_JQz8vH89L56jdabl7fVch1ZAaKPSuCFkCqJC8ykSRUKk8UWrUpMAvHYM21iWYCVSiUMQWpmjZHSqjQBkChm5P5vd-_d14Chz9s6WGwaPX4xhBwSBsAhUzCiD3-o9S4Ej2W-93Wr_SHnLD86zI8O81-HI3x32h1Mi8UZ_TcmfgBdDWw7</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Klein, Marie</creator><creator>Kaestner, Lars</creator><creator>Bogdanova, Anna Y</creator><creator>Minetti, Giampaolo</creator><creator>Rudloff, Silvia</creator><creator>Lundby, Carsten</creator><creator>Makhro, Asya</creator><creator>Seiler, Elena</creator><creator>van Cromvoirt, Ankie</creator><creator>Fenk, Simone</creator><creator>Simionato, Greta</creator><creator>Hertz, Laura</creator><creator>Recktenwald, Steffen</creator><creator>Schäfer, Larissa</creator><creator>Haider, Thomas</creator><creator>Fried, Sebastian</creator><creator>Borsch, Christian</creator><creator>Marti, Hugo H</creator><creator>Sander, Anja</creator><creator>Mairbäurl, Heimo</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1684-0026</orcidid><orcidid>https://orcid.org/0000-0001-5064-4515</orcidid><orcidid>https://orcid.org/0000-0003-0741-1948</orcidid><orcidid>https://orcid.org/0000-0001-7785-2648</orcidid></search><sort><creationdate>20210701</creationdate><title>Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn</title><author>Klein, Marie ; Kaestner, Lars ; Bogdanova, Anna Y ; Minetti, Giampaolo ; Rudloff, Silvia ; Lundby, Carsten ; Makhro, Asya ; Seiler, Elena ; van Cromvoirt, Ankie ; Fenk, Simone ; Simionato, Greta ; Hertz, Laura ; Recktenwald, Steffen ; Schäfer, Larissa ; Haider, Thomas ; Fried, Sebastian ; Borsch, Christian ; Marti, Hugo H ; Sander, Anja ; Mairbäurl, Heimo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-f21d36854de96b78e3b94cec85b524b949ab46d2c68850e26a0cbb66c875226e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klein, Marie</creatorcontrib><creatorcontrib>Kaestner, Lars</creatorcontrib><creatorcontrib>Bogdanova, Anna Y</creatorcontrib><creatorcontrib>Minetti, Giampaolo</creatorcontrib><creatorcontrib>Rudloff, Silvia</creatorcontrib><creatorcontrib>Lundby, Carsten</creatorcontrib><creatorcontrib>Makhro, Asya</creatorcontrib><creatorcontrib>Seiler, Elena</creatorcontrib><creatorcontrib>van Cromvoirt, Ankie</creatorcontrib><creatorcontrib>Fenk, Simone</creatorcontrib><creatorcontrib>Simionato, Greta</creatorcontrib><creatorcontrib>Hertz, Laura</creatorcontrib><creatorcontrib>Recktenwald, Steffen</creatorcontrib><creatorcontrib>Schäfer, Larissa</creatorcontrib><creatorcontrib>Haider, Thomas</creatorcontrib><creatorcontrib>Fried, Sebastian</creatorcontrib><creatorcontrib>Borsch, Christian</creatorcontrib><creatorcontrib>Marti, Hugo H</creatorcontrib><creatorcontrib>Sander, Anja</creatorcontrib><creatorcontrib>Mairbäurl, Heimo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta Physiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klein, Marie</au><au>Kaestner, Lars</au><au>Bogdanova, Anna Y</au><au>Minetti, Giampaolo</au><au>Rudloff, Silvia</au><au>Lundby, Carsten</au><au>Makhro, Asya</au><au>Seiler, Elena</au><au>van Cromvoirt, Ankie</au><au>Fenk, Simone</au><au>Simionato, Greta</au><au>Hertz, Laura</au><au>Recktenwald, Steffen</au><au>Schäfer, Larissa</au><au>Haider, Thomas</au><au>Fried, Sebastian</au><au>Borsch, Christian</au><au>Marti, Hugo H</au><au>Sander, Anja</au><au>Mairbäurl, Heimo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn</atitle><jtitle>Acta Physiologica</jtitle><addtitle>Acta Physiol (Oxf)</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>232</volume><issue>3</issue><spage>e13647</spage><epage>e13647</epage><pages>e13647-e13647</pages><issn>1748-1708</issn><eissn>1748-1716</eissn><abstract>Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a prospective study on erythrocyte survival after a stay at the Jungfraujoch Research Station (JFJRS; 3450 m).
Newly formed erythrocytes of 12 male subjects (mean age 23.3 years) were age cohort labelled in normoxia (110 m) and during a 19-day high-altitude sojourn by ingestion of
C2- and
N-labelled glycine respectively. Elimination dynamics for erythrocytes produced in normoxia and at high altitude were measured by isotope ratio mass spectrometry of haem, by determining tot-Hb, reticulocyte counts, erythrocyte membrane protein 4.1a/4.1b ratio and by mathematical modelling.
Tot-Hb increased by 4.7% ± 2.7% at high altitude and returned to pre-altitude values within 11 days after descent. Elimination of
C- (normoxia) and
N- (high altitude) labelled erythrocytes was not different. Erythropoietin levels and counts of CD71-positive reticulocytes decreased rapidly after descent. The band 4.1a/4.1b ratio decreased at altitude and remained low for 3-4 days after descent and normalized slowly. There was no indication of haemolysis.
We confirm a rapid normalization of tot-Hb upon descent. Based on the lack of accelerated removal of age cohorts of erythrocytes labelled at high altitude, on patterns of changes in reticulocyte counts and of the band 4.1a/4.1b ratio and on modelling, this decrease did not occur via neocytolysis, but by a reduced rate of erythropoiesis along with normal clearance of senescent erythrocytes.</abstract><cop>England</cop><pmid>33729672</pmid><doi>10.1111/apha.13647</doi><orcidid>https://orcid.org/0000-0002-1684-0026</orcidid><orcidid>https://orcid.org/0000-0001-5064-4515</orcidid><orcidid>https://orcid.org/0000-0003-0741-1948</orcidid><orcidid>https://orcid.org/0000-0001-7785-2648</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1748-1708 |
ispartof | Acta Physiologica, 2021-07, Vol.232 (3), p.e13647-e13647 |
issn | 1748-1708 1748-1716 |
language | eng |
recordid | cdi_proquest_miscellaneous_2502212982 |
source | Access via Wiley Online Library |
title | Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T04%3A35%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Absence%20of%20neocytolysis%20in%20humans%20returning%20from%20a%203-week%20high-altitude%20sojourn&rft.jtitle=Acta%20Physiologica&rft.au=Klein,%20Marie&rft.date=2021-07-01&rft.volume=232&rft.issue=3&rft.spage=e13647&rft.epage=e13647&rft.pages=e13647-e13647&rft.issn=1748-1708&rft.eissn=1748-1716&rft_id=info:doi/10.1111/apha.13647&rft_dat=%3Cproquest_cross%3E2502212982%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2502212982&rft_id=info:pmid/33729672&rfr_iscdi=true |