Antifungal activity of polymethyl methacrylate/Si3N4 composites against Candida albicans
Previous studies using gram-positive and -negative bacteria demonstrated that hydrolysis of silicon nitride (Si3N4) in aqueous suspensions elutes nitrogen and produces gaseous ammonia while buffering pH. According to immunochemistry assays, fluorescence imaging, and in situ Raman spectroscopy, we de...
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| Veröffentlicht in: | Acta biomaterialia 2021-05, Vol.126, p.259-276 |
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| creator | Pezzotti, Giuseppe Asai, Tenma Adachi, Tetsuya Ohgitani, Eriko Yamamoto, Toshiro Kanamura, Narisato Boschetto, Francesco Zhu, Wenliang Zanocco, Matteo Marin, Elia Bal, B. Sonny McEntire, Bryan J. Makimura, Koichi Mazda, Osam Nishimura, Ichiro |
| description | Previous studies using gram-positive and -negative bacteria demonstrated that hydrolysis of silicon nitride (Si3N4) in aqueous suspensions elutes nitrogen and produces gaseous ammonia while buffering pH. According to immunochemistry assays, fluorescence imaging, and in situ Raman spectroscopy, we demonstrate here that the antipathogenic surface chemistry of Si3N4 can be extended to polymethylmethacrylate (PMMA) by compounding it with a minor fraction (~8 vol.%) of Si3N4 particles without any tangible loss in bulk properties. The hydrolytic products, which were eluted from partly exposed Si3N4 particles at the composite surface, exhibited fungicidal action against Candida albicans. Using a specific nitrative stress sensing dye and highly resolved fluorescence micrographs, we observed in situ congestion of peroxynitrite (ONOO−) radicals in the mitochondria of the Candida cells exposed to the PMMA/Si3N4 composite, while these radicals were absent in the mitochondria of identical cells exposed to monolithic PMMA. These in situ observations suggest that the surface chemistry of Si3N4 mimics the antifungal activity of macrophages, which concurrently produce NO radicals and superoxide anions (O2•−) resulting in the formation of candidacidal ONOO−. The fungicidal properties of PMMA/Si3N4 composites could be used in dental appliances to inhibit the uncontrolled growth of Candida albicans and ensuing candidiasis while being synergic with chemoprophylaxis.
In a follow-up of previous studies of gram-positive and gram-negative bacteria, we demonstrate here that the antipathogenic surface chemistry of Si3N4 could be extended to polymethylmethacrylate (PMMA) containing a minor fraction (~8 vol.%) of Si3N4 particles without tangible loss in bulk properties. Hydrolytic products eluted from Si3N4 particles at the composite surface exhibited fungicidal action against Candida albicans. Highly resolved fluorescence microscopy revealed congestion of peroxynitrite (ONOO−) radicals in the mitochondria of the Candida cells exposed to the PMMA/Si3N4 composite, while radicals were absent in the mitochondria of identical cells exposed to monolithic PMMA. The fungicidal properties of PMMA/Si3N4 composites could be used in dental appliances to inhibit uncontrolled growth of Candida albicans and ensuing candidiasis in synergy with chemoprophylaxis.
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| doi_str_mv | 10.1016/j.actbio.2021.03.023 |
| format | Article |
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In a follow-up of previous studies of gram-positive and gram-negative bacteria, we demonstrate here that the antipathogenic surface chemistry of Si3N4 could be extended to polymethylmethacrylate (PMMA) containing a minor fraction (~8 vol.%) of Si3N4 particles without tangible loss in bulk properties. Hydrolytic products eluted from Si3N4 particles at the composite surface exhibited fungicidal action against Candida albicans. Highly resolved fluorescence microscopy revealed congestion of peroxynitrite (ONOO−) radicals in the mitochondria of the Candida cells exposed to the PMMA/Si3N4 composite, while radicals were absent in the mitochondria of identical cells exposed to monolithic PMMA. The fungicidal properties of PMMA/Si3N4 composites could be used in dental appliances to inhibit uncontrolled growth of Candida albicans and ensuing candidiasis in synergy with chemoprophylaxis.
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In a follow-up of previous studies of gram-positive and gram-negative bacteria, we demonstrate here that the antipathogenic surface chemistry of Si3N4 could be extended to polymethylmethacrylate (PMMA) containing a minor fraction (~8 vol.%) of Si3N4 particles without tangible loss in bulk properties. Hydrolytic products eluted from Si3N4 particles at the composite surface exhibited fungicidal action against Candida albicans. Highly resolved fluorescence microscopy revealed congestion of peroxynitrite (ONOO−) radicals in the mitochondria of the Candida cells exposed to the PMMA/Si3N4 composite, while radicals were absent in the mitochondria of identical cells exposed to monolithic PMMA. The fungicidal properties of PMMA/Si3N4 composites could be used in dental appliances to inhibit uncontrolled growth of Candida albicans and ensuing candidiasis in synergy with chemoprophylaxis.
[Display omitted]</description><subject>Ammonia</subject><subject>Anions</subject><subject>Antifungal activity</subject><subject>Bacteria</subject><subject>Buffers (chemistry)</subject><subject>Candida</subject><subject>Candida albicans</subject><subject>Candidacidal activity</subject><subject>Candidiasis</subject><subject>Congestion</subject><subject>Dental prostheses</subject><subject>Dental restorative materials</subject><subject>Exposure</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Fungal sterol</subject><subject>Fungicides</subject><subject>Gram-negative bacteria</subject><subject>Macrophages</subject><subject>Micrography</subject><subject>Mitochondria</subject><subject>Particulate composites</subject><subject>Peroxynitrite</subject><subject>Photomicrographs</subject><subject>PMMA/Si3N4 composites</subject><subject>Polymethyl methacrylate</subject><subject>Polymethylmethacrylate</subject><subject>Radicals</subject><subject>Raman spectroscopy</subject><subject>Reactive nitrogen species</subject><subject>Silicon nitride</subject><subject>Superoxide anions</subject><subject>Surface chemistry</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhiMEEqXwDxgisbAkPdtp7SxIVcWXVMEASGzWxXaKqzQutlsp_x5XZWJguhue99Xdk2XXBEoCZDZZl6hiY11JgZISWAmUnWQjIrgo-HQmTtPOK1pwmJHz7CKENQAThIpR9jnvo213_Qq7PJXYvY1D7tp867phY-LX0OWHgcoPHUYzebPspcqV22xdsNGEHFdo-xDzBfbaasyxa6zCPlxmZy12wVz9znH28XD_vngqlq-Pz4v5slAVr2NRIatmpgFeM91qpjVCawimryg2AAic8amiWqlaiZZNG0GwolxxQaGumoaNs9tj79a7750JUW5sUKbrsDduFySdAqWE1DVP6M0fdO12vk_XJYpRnnxRkajqSCnvQvCmlVtvN-gHSUAedMu1POqWB90SmEy6U-zuGDPp2b01XgZlTa-Mtt6oKLWz_xf8AJhOit4</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Pezzotti, Giuseppe</creator><creator>Asai, Tenma</creator><creator>Adachi, Tetsuya</creator><creator>Ohgitani, Eriko</creator><creator>Yamamoto, Toshiro</creator><creator>Kanamura, Narisato</creator><creator>Boschetto, Francesco</creator><creator>Zhu, Wenliang</creator><creator>Zanocco, Matteo</creator><creator>Marin, Elia</creator><creator>Bal, B. Sonny</creator><creator>McEntire, Bryan J.</creator><creator>Makimura, Koichi</creator><creator>Mazda, Osam</creator><creator>Nishimura, Ichiro</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0981-7821</orcidid></search><sort><creationdate>202105</creationdate><title>Antifungal activity of polymethyl methacrylate/Si3N4 composites against Candida albicans</title><author>Pezzotti, Giuseppe ; Asai, Tenma ; Adachi, Tetsuya ; Ohgitani, Eriko ; Yamamoto, Toshiro ; Kanamura, Narisato ; Boschetto, Francesco ; Zhu, Wenliang ; Zanocco, Matteo ; Marin, Elia ; Bal, B. 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Sonny</au><au>McEntire, Bryan J.</au><au>Makimura, Koichi</au><au>Mazda, Osam</au><au>Nishimura, Ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antifungal activity of polymethyl methacrylate/Si3N4 composites against Candida albicans</atitle><jtitle>Acta biomaterialia</jtitle><date>2021-05</date><risdate>2021</risdate><volume>126</volume><spage>259</spage><epage>276</epage><pages>259-276</pages><issn>1742-7061</issn><eissn>1878-7568</eissn><abstract>Previous studies using gram-positive and -negative bacteria demonstrated that hydrolysis of silicon nitride (Si3N4) in aqueous suspensions elutes nitrogen and produces gaseous ammonia while buffering pH. According to immunochemistry assays, fluorescence imaging, and in situ Raman spectroscopy, we demonstrate here that the antipathogenic surface chemistry of Si3N4 can be extended to polymethylmethacrylate (PMMA) by compounding it with a minor fraction (~8 vol.%) of Si3N4 particles without any tangible loss in bulk properties. The hydrolytic products, which were eluted from partly exposed Si3N4 particles at the composite surface, exhibited fungicidal action against Candida albicans. Using a specific nitrative stress sensing dye and highly resolved fluorescence micrographs, we observed in situ congestion of peroxynitrite (ONOO−) radicals in the mitochondria of the Candida cells exposed to the PMMA/Si3N4 composite, while these radicals were absent in the mitochondria of identical cells exposed to monolithic PMMA. These in situ observations suggest that the surface chemistry of Si3N4 mimics the antifungal activity of macrophages, which concurrently produce NO radicals and superoxide anions (O2•−) resulting in the formation of candidacidal ONOO−. The fungicidal properties of PMMA/Si3N4 composites could be used in dental appliances to inhibit the uncontrolled growth of Candida albicans and ensuing candidiasis while being synergic with chemoprophylaxis.
In a follow-up of previous studies of gram-positive and gram-negative bacteria, we demonstrate here that the antipathogenic surface chemistry of Si3N4 could be extended to polymethylmethacrylate (PMMA) containing a minor fraction (~8 vol.%) of Si3N4 particles without tangible loss in bulk properties. Hydrolytic products eluted from Si3N4 particles at the composite surface exhibited fungicidal action against Candida albicans. Highly resolved fluorescence microscopy revealed congestion of peroxynitrite (ONOO−) radicals in the mitochondria of the Candida cells exposed to the PMMA/Si3N4 composite, while radicals were absent in the mitochondria of identical cells exposed to monolithic PMMA. The fungicidal properties of PMMA/Si3N4 composites could be used in dental appliances to inhibit uncontrolled growth of Candida albicans and ensuing candidiasis in synergy with chemoprophylaxis.
[Display omitted]</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.actbio.2021.03.023</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-0981-7821</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Acta biomaterialia, 2021-05, Vol.126, p.259-276 |
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| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Ammonia Anions Antifungal activity Bacteria Buffers (chemistry) Candida Candida albicans Candidacidal activity Candidiasis Congestion Dental prostheses Dental restorative materials Exposure Fluorescence Fluorescence microscopy Fungal sterol Fungicides Gram-negative bacteria Macrophages Micrography Mitochondria Particulate composites Peroxynitrite Photomicrographs PMMA/Si3N4 composites Polymethyl methacrylate Polymethylmethacrylate Radicals Raman spectroscopy Reactive nitrogen species Silicon nitride Superoxide anions Surface chemistry |
| title | Antifungal activity of polymethyl methacrylate/Si3N4 composites against Candida albicans |
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