Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis
Non-invasive early diagnosis is of great significance in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. High-throughput, sensitive, economic, and fast miRNA sensing platforms a...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2021-03, Vol.93 (12), p.5129-5136 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5136 |
|---|---|
| container_issue | 12 |
| container_start_page | 5129 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 93 |
| creator | Chu, Yujin Gao, Yakun Tang, Wei Qiang, Le Han, Yingkuan Gao, Jianwei Zhang, Yu Liu, Hong Han, Lin |
| description | Non-invasive early diagnosis is of great significance in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. High-throughput, sensitive, economic, and fast miRNA sensing platforms are necessary to realize the low-concentration miRNA detection in clinical diagnosis and biological studies. Here, we developed an attomolar-level ultrasensitive, rapid, and multiple-miRNA simultaneous detection platform enabled by nanomaterial locally assembled microfluidic biochips. This platform presents a large linear detection regime of 1 aM–10 nM, an ultralow detection limit of 0.146 aM with no amplification, a short detection time of 35 min with multiplex miRNA sensing capability, and a small sample volume consumption of 2 μL. The detection results of five miRNAs in real samples from breast cancer patients and healthy humans indicate its excellent capacity for practical applications in early cancer diagnosis. The proposed ultrasensitive, rapid, and multiple-miRNA detection microfluidic biochip platform is a universal miRNA detection approach and an important and valuable tool in early cancer screening and diagnosis as well as biological studies. |
| doi_str_mv | 10.1021/acs.analchem.0c04896 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2501852956</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2501852956</sourcerecordid><originalsourceid>FETCH-LOGICAL-a376t-594fb7f1694f62a4574dbcb4499cd4fac1515e256c1f9d9ecf2cc96425b522b53</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi0EokvhHyBkiQuXLLZjO8lx2ZYPaVskRM_RxBlTV0682E7F_hD-Lwm77YEDp7k87zuaeQh5zdmaM8Hfg0lrGMGbWxzWzDBZN_oJWXElWKHrWjwlK8ZYWYiKsTPyIqU7xjhnXD8nZ2VZCVYxvSK_NzmHIXiIxQ7v0dMbnyMkHJPL7h4pjD29mnx2e4-_6OBMDN-uN_QCM5rswkgvR-g89rQ7UKDXMIYBMkYHnu6CAe8PdJMSDn-ZqyVu_eR6Z-gHF8yt21MbIt3CaDDSCwc_xpBcekmeWfAJX53mObn5ePl9-7nYff30ZbvZFVBWOheqkbarLNfz1AKkqmTfmU7KpjG9tGC44gqF0obbpm_QWGFMo6VQnRKiU-U5eXfs3cfwc8KU28Elg97DiGFKrVCM10o0Ss_o23_QuzDF-f8LxWspBJdLoTxS86EpRbTtProB4qHlrF20tbO29kFbe9I2x96cyqduwP4x9OBpBtgRWOKPi__b-QdvrqkF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2518422145</pqid></control><display><type>article</type><title>Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis</title><source>ACS Publications</source><creator>Chu, Yujin ; Gao, Yakun ; Tang, Wei ; Qiang, Le ; Han, Yingkuan ; Gao, Jianwei ; Zhang, Yu ; Liu, Hong ; Han, Lin</creator><creatorcontrib>Chu, Yujin ; Gao, Yakun ; Tang, Wei ; Qiang, Le ; Han, Yingkuan ; Gao, Jianwei ; Zhang, Yu ; Liu, Hong ; Han, Lin</creatorcontrib><description>Non-invasive early diagnosis is of great significance in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. High-throughput, sensitive, economic, and fast miRNA sensing platforms are necessary to realize the low-concentration miRNA detection in clinical diagnosis and biological studies. Here, we developed an attomolar-level ultrasensitive, rapid, and multiple-miRNA simultaneous detection platform enabled by nanomaterial locally assembled microfluidic biochips. This platform presents a large linear detection regime of 1 aM–10 nM, an ultralow detection limit of 0.146 aM with no amplification, a short detection time of 35 min with multiplex miRNA sensing capability, and a small sample volume consumption of 2 μL. The detection results of five miRNAs in real samples from breast cancer patients and healthy humans indicate its excellent capacity for practical applications in early cancer diagnosis. The proposed ultrasensitive, rapid, and multiple-miRNA detection microfluidic biochip platform is a universal miRNA detection approach and an important and valuable tool in early cancer screening and diagnosis as well as biological studies.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.0c04896</identifier><identifier>PMID: 33720706</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biochips ; Breast cancer ; Cancer ; Cancer screening ; Chemistry ; Diagnosis ; Medical diagnosis ; Medical screening ; Medical treatment ; Microfluidics ; MicroRNAs ; miRNA ; Multiplexing ; Nanomaterials ; Ribonucleic acid ; RNA</subject><ispartof>Analytical chemistry (Washington), 2021-03, Vol.93 (12), p.5129-5136</ispartof><rights>2021 American Chemical Society</rights><rights>Copyright American Chemical Society Mar 30, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-594fb7f1694f62a4574dbcb4499cd4fac1515e256c1f9d9ecf2cc96425b522b53</citedby><cites>FETCH-LOGICAL-a376t-594fb7f1694f62a4574dbcb4499cd4fac1515e256c1f9d9ecf2cc96425b522b53</cites><orcidid>0000-0002-0666-2537 ; 0000-0003-1640-9620 ; 0000-0003-0461-2031</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c04896$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c04896$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,777,781,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33720706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chu, Yujin</creatorcontrib><creatorcontrib>Gao, Yakun</creatorcontrib><creatorcontrib>Tang, Wei</creatorcontrib><creatorcontrib>Qiang, Le</creatorcontrib><creatorcontrib>Han, Yingkuan</creatorcontrib><creatorcontrib>Gao, Jianwei</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Han, Lin</creatorcontrib><title>Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Non-invasive early diagnosis is of great significance in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. High-throughput, sensitive, economic, and fast miRNA sensing platforms are necessary to realize the low-concentration miRNA detection in clinical diagnosis and biological studies. Here, we developed an attomolar-level ultrasensitive, rapid, and multiple-miRNA simultaneous detection platform enabled by nanomaterial locally assembled microfluidic biochips. This platform presents a large linear detection regime of 1 aM–10 nM, an ultralow detection limit of 0.146 aM with no amplification, a short detection time of 35 min with multiplex miRNA sensing capability, and a small sample volume consumption of 2 μL. The detection results of five miRNAs in real samples from breast cancer patients and healthy humans indicate its excellent capacity for practical applications in early cancer diagnosis. The proposed ultrasensitive, rapid, and multiple-miRNA detection microfluidic biochip platform is a universal miRNA detection approach and an important and valuable tool in early cancer screening and diagnosis as well as biological studies.</description><subject>Biochips</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cancer screening</subject><subject>Chemistry</subject><subject>Diagnosis</subject><subject>Medical diagnosis</subject><subject>Medical screening</subject><subject>Medical treatment</subject><subject>Microfluidics</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Multiplexing</subject><subject>Nanomaterials</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kU1v1DAQhi0EokvhHyBkiQuXLLZjO8lx2ZYPaVskRM_RxBlTV0682E7F_hD-Lwm77YEDp7k87zuaeQh5zdmaM8Hfg0lrGMGbWxzWzDBZN_oJWXElWKHrWjwlK8ZYWYiKsTPyIqU7xjhnXD8nZ2VZCVYxvSK_NzmHIXiIxQ7v0dMbnyMkHJPL7h4pjD29mnx2e4-_6OBMDN-uN_QCM5rswkgvR-g89rQ7UKDXMIYBMkYHnu6CAe8PdJMSDn-ZqyVu_eR6Z-gHF8yt21MbIt3CaDDSCwc_xpBcekmeWfAJX53mObn5ePl9-7nYff30ZbvZFVBWOheqkbarLNfz1AKkqmTfmU7KpjG9tGC44gqF0obbpm_QWGFMo6VQnRKiU-U5eXfs3cfwc8KU28Elg97DiGFKrVCM10o0Ss_o23_QuzDF-f8LxWspBJdLoTxS86EpRbTtProB4qHlrF20tbO29kFbe9I2x96cyqduwP4x9OBpBtgRWOKPi__b-QdvrqkF</recordid><startdate>20210330</startdate><enddate>20210330</enddate><creator>Chu, Yujin</creator><creator>Gao, Yakun</creator><creator>Tang, Wei</creator><creator>Qiang, Le</creator><creator>Han, Yingkuan</creator><creator>Gao, Jianwei</creator><creator>Zhang, Yu</creator><creator>Liu, Hong</creator><creator>Han, Lin</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0666-2537</orcidid><orcidid>https://orcid.org/0000-0003-1640-9620</orcidid><orcidid>https://orcid.org/0000-0003-0461-2031</orcidid></search><sort><creationdate>20210330</creationdate><title>Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis</title><author>Chu, Yujin ; Gao, Yakun ; Tang, Wei ; Qiang, Le ; Han, Yingkuan ; Gao, Jianwei ; Zhang, Yu ; Liu, Hong ; Han, Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-594fb7f1694f62a4574dbcb4499cd4fac1515e256c1f9d9ecf2cc96425b522b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biochips</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cancer screening</topic><topic>Chemistry</topic><topic>Diagnosis</topic><topic>Medical diagnosis</topic><topic>Medical screening</topic><topic>Medical treatment</topic><topic>Microfluidics</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Multiplexing</topic><topic>Nanomaterials</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chu, Yujin</creatorcontrib><creatorcontrib>Gao, Yakun</creatorcontrib><creatorcontrib>Tang, Wei</creatorcontrib><creatorcontrib>Qiang, Le</creatorcontrib><creatorcontrib>Han, Yingkuan</creatorcontrib><creatorcontrib>Gao, Jianwei</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Han, Lin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chu, Yujin</au><au>Gao, Yakun</au><au>Tang, Wei</au><au>Qiang, Le</au><au>Han, Yingkuan</au><au>Gao, Jianwei</au><au>Zhang, Yu</au><au>Liu, Hong</au><au>Han, Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2021-03-30</date><risdate>2021</risdate><volume>93</volume><issue>12</issue><spage>5129</spage><epage>5136</epage><pages>5129-5136</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Non-invasive early diagnosis is of great significance in disease pathologic development and subsequent medical treatments, and microRNA (miRNA) detection has attracted critical attention in early cancer screening and diagnosis. High-throughput, sensitive, economic, and fast miRNA sensing platforms are necessary to realize the low-concentration miRNA detection in clinical diagnosis and biological studies. Here, we developed an attomolar-level ultrasensitive, rapid, and multiple-miRNA simultaneous detection platform enabled by nanomaterial locally assembled microfluidic biochips. This platform presents a large linear detection regime of 1 aM–10 nM, an ultralow detection limit of 0.146 aM with no amplification, a short detection time of 35 min with multiplex miRNA sensing capability, and a small sample volume consumption of 2 μL. The detection results of five miRNAs in real samples from breast cancer patients and healthy humans indicate its excellent capacity for practical applications in early cancer diagnosis. The proposed ultrasensitive, rapid, and multiple-miRNA detection microfluidic biochip platform is a universal miRNA detection approach and an important and valuable tool in early cancer screening and diagnosis as well as biological studies.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>33720706</pmid><doi>10.1021/acs.analchem.0c04896</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-0666-2537</orcidid><orcidid>https://orcid.org/0000-0003-1640-9620</orcidid><orcidid>https://orcid.org/0000-0003-0461-2031</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2021-03, Vol.93 (12), p.5129-5136 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2501852956 |
| source | ACS Publications |
| subjects | Biochips Breast cancer Cancer Cancer screening Chemistry Diagnosis Medical diagnosis Medical screening Medical treatment Microfluidics MicroRNAs miRNA Multiplexing Nanomaterials Ribonucleic acid RNA |
| title | Attomolar-Level Ultrasensitive and Multiplex microRNA Detection Enabled by a Nanomaterial Locally Assembled Microfluidic Biochip for Cancer Diagnosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T06%3A08%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Attomolar-Level%20Ultrasensitive%20and%20Multiplex%20microRNA%20Detection%20Enabled%20by%20a%20Nanomaterial%20Locally%20Assembled%20Microfluidic%20Biochip%20for%20Cancer%20Diagnosis&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Chu,%20Yujin&rft.date=2021-03-30&rft.volume=93&rft.issue=12&rft.spage=5129&rft.epage=5136&rft.pages=5129-5136&rft.issn=0003-2700&rft.eissn=1520-6882&rft_id=info:doi/10.1021/acs.analchem.0c04896&rft_dat=%3Cproquest_cross%3E2501852956%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2518422145&rft_id=info:pmid/33720706&rfr_iscdi=true |