A rapid method for solubilization of chimeric human interferon regulatory factor-1 (IRF-1) protein in Escherichia coli

Interferon regulatory factor-1 (IRF-1) is a vertebrate transcription factor that plays significant roles in cell cycle regulation, anti-viral response, tumor suppression and immune response. High-level expression of recombinant IRF-1 at 37 °C leads to the formation of insoluble aggregates (insoluble...

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Veröffentlicht in:	Molecular biology reports 2021-03, Vol.48 (3), p.2307-2314
Hauptverfasser:	Mishra, Santosh Kumar, Verma, Ruchi, Kumari, Priyanka, Kumari, Anjali, Prakash, Krishna
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Animal Anatomy Animal Biochemistry Antiviral agents Arginine Biological activity Biomedical and Life Sciences Cell cycle Degradation products DNA - metabolism E coli Electrophoretic mobility Escherichia coli Escherichia coli - isolation & purification Escherichia coli - metabolism Gene regulation Glutathione transferase Histology Humans Immune response Interferon Interferon regulatory factor Interferon regulatory factor 1 Interferon Regulatory Factor-1 - chemistry Life Sciences Lysis Mannitol Morphology Original Article Polyclonal antibodies Proline Protein Binding Protein purification Proteolysis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Solubility Solubilization Tumor suppression β-Interferon
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