DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication
•DPV pUL41 degrades RNA polymerase (Pol) II-transcribed translatable RNA and induces protein synthesis shutoff.•DPV CHv-BAC-ΔUL41 mutant virus exhibits a significant viral growth defect and plaque size reduction in DEF cells.•DPV pUL41 significantly affects the viral DNA replication and viral releas...
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| Veröffentlicht in: | Veterinary microbiology 2021-04, Vol.255, p.108979-108979, Article 108979 |
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| container_title | Veterinary microbiology |
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| creator | He, Tianqiong Wang, Mingshu Cheng, Anchun Yang, Qiao Jia, Renyong Wu, Ying Huang, Juan Tian, Bin Liu, Mafeng Chen, Shun Zhao, Xin-Xin Zhu, Dekang Zhang, Shaqiu Ou, Xuming Mao, Sai Gao, Qun Sun, Di |
| description | •DPV pUL41 degrades RNA polymerase (Pol) II-transcribed translatable RNA and induces protein synthesis shutoff.•DPV CHv-BAC-ΔUL41 mutant virus exhibits a significant viral growth defect and plaque size reduction in DEF cells.•DPV pUL41 significantly affects the viral DNA replication and viral release.•DPV pUL41 regulates viral mRNA accumulation.
The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread. |
| doi_str_mv | 10.1016/j.vetmic.2021.108979 |
| format | Article |
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The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2021.108979</identifier><identifier>PMID: 33721633</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>DNA biosynthesis ; DNA-directed RNA polymerase ; DPV ; Duck plague ; Herpes simplex ; mRNA ; Plague ; Protein biosynthesis ; Proteins ; Replication ; RNA polymerase ; Transcription ; UL41 ; UL41 gene ; VHS ; Viral replication ; Virions ; Viruses</subject><ispartof>Veterinary microbiology, 2021-04, Vol.255, p.108979-108979, Article 108979</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Apr 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-f56bfc06cfff19c2e2d9baa53f02dc64dd8451bce4f34f7f1459330209f797373</citedby><cites>FETCH-LOGICAL-c390t-f56bfc06cfff19c2e2d9baa53f02dc64dd8451bce4f34f7f1459330209f797373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2021.108979$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33721633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Tianqiong</creatorcontrib><creatorcontrib>Wang, Mingshu</creatorcontrib><creatorcontrib>Cheng, Anchun</creatorcontrib><creatorcontrib>Yang, Qiao</creatorcontrib><creatorcontrib>Jia, Renyong</creatorcontrib><creatorcontrib>Wu, Ying</creatorcontrib><creatorcontrib>Huang, Juan</creatorcontrib><creatorcontrib>Tian, Bin</creatorcontrib><creatorcontrib>Liu, Mafeng</creatorcontrib><creatorcontrib>Chen, Shun</creatorcontrib><creatorcontrib>Zhao, Xin-Xin</creatorcontrib><creatorcontrib>Zhu, Dekang</creatorcontrib><creatorcontrib>Zhang, Shaqiu</creatorcontrib><creatorcontrib>Ou, Xuming</creatorcontrib><creatorcontrib>Mao, Sai</creatorcontrib><creatorcontrib>Gao, Qun</creatorcontrib><creatorcontrib>Sun, Di</creatorcontrib><title>DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>•DPV pUL41 degrades RNA polymerase (Pol) II-transcribed translatable RNA and induces protein synthesis shutoff.•DPV CHv-BAC-ΔUL41 mutant virus exhibits a significant viral growth defect and plaque size reduction in DEF cells.•DPV pUL41 significantly affects the viral DNA replication and viral release.•DPV pUL41 regulates viral mRNA accumulation.
The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread.</description><subject>DNA biosynthesis</subject><subject>DNA-directed RNA polymerase</subject><subject>DPV</subject><subject>Duck plague</subject><subject>Herpes simplex</subject><subject>mRNA</subject><subject>Plague</subject><subject>Protein biosynthesis</subject><subject>Proteins</subject><subject>Replication</subject><subject>RNA polymerase</subject><subject>Transcription</subject><subject>UL41</subject><subject>UL41 gene</subject><subject>VHS</subject><subject>Viral replication</subject><subject>Virions</subject><subject>Viruses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LXDEUhkNR6mj7D0oJuOnmjvm6H9kIResHDNhF7aKbkElONMOdZExyB_z3jVzrogtXBw7P-57Dg9AXSpaU0O5ss9xD2XqzZITRuhpkLz-gBR163rBWsAO0ILwfGkp5e4SOc94QQoTsyEd0xHnPaMf5Av25_Pkb368ExQ8QAEMw0frwgHcpFvAB-2AnAxk_xlxwfpxKdA5rU_zel2esg8XaOTAl471PesQJdqM3uvgYPqFDp8cMn1_nCbq_-vHr4qZZ3V3fXnxfNYZLUhrXdmtnSGecc1QaBszKtdYtd4RZ0wlrB9HStQHhuHC9o6KVnBNGpOtlz3t-gr7NvfXnpwlyUVufDYyjDhCnrFhL6CCqHV7R0__QTZxSqN9VinWS1D5WKTFTJsWcEzi1S36r07OiRL24Vxs1u1cv7tXsvsa-vpZP6y3Yt9A_2RU4nwGoNvYeksrGV-NgfaoKlY3-_Qt_AaFVloU</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>He, Tianqiong</creator><creator>Wang, Mingshu</creator><creator>Cheng, Anchun</creator><creator>Yang, Qiao</creator><creator>Jia, Renyong</creator><creator>Wu, Ying</creator><creator>Huang, Juan</creator><creator>Tian, Bin</creator><creator>Liu, Mafeng</creator><creator>Chen, Shun</creator><creator>Zhao, Xin-Xin</creator><creator>Zhu, Dekang</creator><creator>Zhang, Shaqiu</creator><creator>Ou, Xuming</creator><creator>Mao, Sai</creator><creator>Gao, Qun</creator><creator>Sun, Di</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>202104</creationdate><title>DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication</title><author>He, Tianqiong ; Wang, Mingshu ; Cheng, Anchun ; Yang, Qiao ; Jia, Renyong ; Wu, Ying ; Huang, Juan ; Tian, Bin ; Liu, Mafeng ; Chen, Shun ; Zhao, Xin-Xin ; Zhu, Dekang ; Zhang, Shaqiu ; Ou, Xuming ; Mao, Sai ; Gao, Qun ; Sun, Di</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-f56bfc06cfff19c2e2d9baa53f02dc64dd8451bce4f34f7f1459330209f797373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>DNA biosynthesis</topic><topic>DNA-directed RNA polymerase</topic><topic>DPV</topic><topic>Duck plague</topic><topic>Herpes simplex</topic><topic>mRNA</topic><topic>Plague</topic><topic>Protein biosynthesis</topic><topic>Proteins</topic><topic>Replication</topic><topic>RNA polymerase</topic><topic>Transcription</topic><topic>UL41</topic><topic>UL41 gene</topic><topic>VHS</topic><topic>Viral replication</topic><topic>Virions</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Tianqiong</creatorcontrib><creatorcontrib>Wang, Mingshu</creatorcontrib><creatorcontrib>Cheng, Anchun</creatorcontrib><creatorcontrib>Yang, Qiao</creatorcontrib><creatorcontrib>Jia, Renyong</creatorcontrib><creatorcontrib>Wu, Ying</creatorcontrib><creatorcontrib>Huang, Juan</creatorcontrib><creatorcontrib>Tian, Bin</creatorcontrib><creatorcontrib>Liu, Mafeng</creatorcontrib><creatorcontrib>Chen, Shun</creatorcontrib><creatorcontrib>Zhao, Xin-Xin</creatorcontrib><creatorcontrib>Zhu, Dekang</creatorcontrib><creatorcontrib>Zhang, Shaqiu</creatorcontrib><creatorcontrib>Ou, Xuming</creatorcontrib><creatorcontrib>Mao, Sai</creatorcontrib><creatorcontrib>Gao, Qun</creatorcontrib><creatorcontrib>Sun, Di</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Tianqiong</au><au>Wang, Mingshu</au><au>Cheng, Anchun</au><au>Yang, Qiao</au><au>Jia, Renyong</au><au>Wu, Ying</au><au>Huang, Juan</au><au>Tian, Bin</au><au>Liu, Mafeng</au><au>Chen, Shun</au><au>Zhao, Xin-Xin</au><au>Zhu, Dekang</au><au>Zhang, Shaqiu</au><au>Ou, Xuming</au><au>Mao, Sai</au><au>Gao, Qun</au><au>Sun, Di</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2021-04</date><risdate>2021</risdate><volume>255</volume><spage>108979</spage><epage>108979</epage><pages>108979-108979</pages><artnum>108979</artnum><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>•DPV pUL41 degrades RNA polymerase (Pol) II-transcribed translatable RNA and induces protein synthesis shutoff.•DPV CHv-BAC-ΔUL41 mutant virus exhibits a significant viral growth defect and plaque size reduction in DEF cells.•DPV pUL41 significantly affects the viral DNA replication and viral release.•DPV pUL41 regulates viral mRNA accumulation.
The virion host shutoff (VHS) protein, encoded by the UL41 gene of herpes simplex virus (HSV), specifically degrades mRNA and induces host shutoff. VHS and its homologs are highly conserved in the Alphaherpesvirinae subfamily. However, the role of the duck plague virus (DPV) UL41 gene is unclear. In this study, we found that the DPV UL41 gene-encoded protein (pUL41) degrades RNA polymerase (pol) II-transcribed translatable RNA and induces protein synthesis shutoff. DPV pUL41 was dispensable for viral replication, but the UL41-deleted mutant virus exhibited a significant viral growth defect and plaque size reduction in Duck embryo fibroblast (DEF) cells. Furthermore, DPV pUL41 regulated viral mRNA accumulation to affect viral DNA replication, release and cell-to-cell spread.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33721633</pmid><doi>10.1016/j.vetmic.2021.108979</doi><tpages>1</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | DNA biosynthesis DNA-directed RNA polymerase DPV Duck plague Herpes simplex mRNA Plague Protein biosynthesis Proteins Replication RNA polymerase Transcription UL41 UL41 gene VHS Viral replication Virions Viruses |
| title | DPV UL41 gene encoding protein induces host shutoff activity and affects viral replication |
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