Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells
This study aimed to evaluate the effects of low-energy red light-emitting diode (LED) irradiation on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). PDLSCs were derived from human periodontal ligament tissues of premolars and were irradiated with 0 (cont...
Gespeichert in:
| Veröffentlicht in: | Lasers in medical science 2021-09, Vol.36 (7), p.1535-1543 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1543 |
|---|---|
| container_issue | 7 |
| container_start_page | 1535 |
| container_title | Lasers in medical science |
| container_volume | 36 |
| creator | Wu, Yan Zhu, Tingting Yang, Yaoyao Gao, Hong Shu, Chunxia Chen, Qiang Yang, Juan Luo, Xiang Wang, Yao |
| description | This study aimed to evaluate the effects of low-energy red light-emitting diode (LED) irradiation on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). PDLSCs were derived from human periodontal ligament tissues of premolars and were irradiated with 0 (control group), 1, 3, or 5 J/cm
2
red LED in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) results. Osteoblast-associated proteins (Runx2, OCN, OPN, and BSP) were detected using western blotting. The results of the MTT assay indicated that PDLSCs in the irradiation groups exhibited a higher proliferation rate than those in the control group (
P
< 0.05). ALP results showed that after 7 days of illumination, only 5 J/cm
2
promoted the expression of ALP of PDLSCs. However, after 14 days of illumination, the irradiation treatments did not increase ALP activity. The results of alizarin red staining showed that red LED promoted osteogenic differentiation of the PDLSCs. The real-time polymerase chain reaction (RT-PCR) results demonstrated that red LED upregulated the expression levels of osteogenic genes. Expression of the proteins BSP, OPN, OCN, and Runx2 in the irradiation groups was higher than that in the control group. Our results confirmed that low-energy red LED at 1, 3, and 5 J/cm
2
promotes proliferation and osteogenic differentiation of PDLSCs. |
| doi_str_mv | 10.1007/s10103-021-03278-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2501485801</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2559950687</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-36b55924c9f05b14e35ed000eba1a61c8bd5b58981e5207a67f1fee3b77606773</originalsourceid><addsrcrecordid>eNp9kUGL1TAUhYMozpvRP-BCAm7cVO9NmqZdyjDqwIAbBXchbW_fy9AmzyQPcesvN7XPEVy4yuJ858uFw9gLhDcIoN8mBARZgcAKpNBthY_YDmupqgbqr4_ZDkTTVm0n8IJdpnQPgLpB-ZRdSKmxAwE79vM2Rjs6m13w_LvLBx5p5LPbH3JFi8vZ-T0fXRiJkz9YP1DixxhmN1HcStaPPKRMYU_eDYWdSkQ-n51h4keKRRB8tvNqtktJeWksfKB5Ts_Yk8nOiZ6f3yv25f3N5-uP1d2nD7fX7-6qQWqVK9n0SnWiHroJVI81SUUjAFBv0TY4tP2oetV2LZISoG2jJ5yIZK91A43W8oq93rzl_m8nStksLq0XWE_hlIxQgHWrWsCCvvoHvQ-n6Mt1hVJdp6BpV6HYqCGGlCJN5hjdYuMPg2DWhcy2kCkLmd8LmVX98qw-9QuND5U_kxRAbkAqkd9T_Pv3f7S_AOLYnbc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2559950687</pqid></control><display><type>article</type><title>Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells</title><source>SpringerLink Journals - AutoHoldings</source><creator>Wu, Yan ; Zhu, Tingting ; Yang, Yaoyao ; Gao, Hong ; Shu, Chunxia ; Chen, Qiang ; Yang, Juan ; Luo, Xiang ; Wang, Yao</creator><creatorcontrib>Wu, Yan ; Zhu, Tingting ; Yang, Yaoyao ; Gao, Hong ; Shu, Chunxia ; Chen, Qiang ; Yang, Juan ; Luo, Xiang ; Wang, Yao</creatorcontrib><description>This study aimed to evaluate the effects of low-energy red light-emitting diode (LED) irradiation on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). PDLSCs were derived from human periodontal ligament tissues of premolars and were irradiated with 0 (control group), 1, 3, or 5 J/cm
2
red LED in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) results. Osteoblast-associated proteins (Runx2, OCN, OPN, and BSP) were detected using western blotting. The results of the MTT assay indicated that PDLSCs in the irradiation groups exhibited a higher proliferation rate than those in the control group (
P
< 0.05). ALP results showed that after 7 days of illumination, only 5 J/cm
2
promoted the expression of ALP of PDLSCs. However, after 14 days of illumination, the irradiation treatments did not increase ALP activity. The results of alizarin red staining showed that red LED promoted osteogenic differentiation of the PDLSCs. The real-time polymerase chain reaction (RT-PCR) results demonstrated that red LED upregulated the expression levels of osteogenic genes. Expression of the proteins BSP, OPN, OCN, and Runx2 in the irradiation groups was higher than that in the control group. Our results confirmed that low-energy red LED at 1, 3, and 5 J/cm
2
promotes proliferation and osteogenic differentiation of PDLSCs.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-021-03278-1</identifier><identifier>PMID: 33719020</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Alizarin ; Alkaline phosphatase ; Biomedical materials ; Cbfa-1 protein ; Cell differentiation ; Cell proliferation ; Dentistry ; Differentiation (biology) ; Gene expression ; Illumination ; Irradiation ; Lasers ; Ligaments ; Light effects ; Light emitting diodes ; Medicine ; Medicine & Public Health ; Mesenchymal stem cells ; Optical Devices ; Optics ; Original Article ; Periodontal ligament ; Photonics ; Polymerase chain reaction ; Premolars ; Proteins ; Quantum Optics ; Real time ; Staining ; Stem cells ; Western blotting</subject><ispartof>Lasers in medical science, 2021-09, Vol.36 (7), p.1535-1543</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-36b55924c9f05b14e35ed000eba1a61c8bd5b58981e5207a67f1fee3b77606773</citedby><cites>FETCH-LOGICAL-c375t-36b55924c9f05b14e35ed000eba1a61c8bd5b58981e5207a67f1fee3b77606773</cites><orcidid>0000-0001-8400-1665</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-021-03278-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-021-03278-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33719020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Yan</creatorcontrib><creatorcontrib>Zhu, Tingting</creatorcontrib><creatorcontrib>Yang, Yaoyao</creatorcontrib><creatorcontrib>Gao, Hong</creatorcontrib><creatorcontrib>Shu, Chunxia</creatorcontrib><creatorcontrib>Chen, Qiang</creatorcontrib><creatorcontrib>Yang, Juan</creatorcontrib><creatorcontrib>Luo, Xiang</creatorcontrib><creatorcontrib>Wang, Yao</creatorcontrib><title>Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>This study aimed to evaluate the effects of low-energy red light-emitting diode (LED) irradiation on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). PDLSCs were derived from human periodontal ligament tissues of premolars and were irradiated with 0 (control group), 1, 3, or 5 J/cm
2
red LED in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) results. Osteoblast-associated proteins (Runx2, OCN, OPN, and BSP) were detected using western blotting. The results of the MTT assay indicated that PDLSCs in the irradiation groups exhibited a higher proliferation rate than those in the control group (
P
< 0.05). ALP results showed that after 7 days of illumination, only 5 J/cm
2
promoted the expression of ALP of PDLSCs. However, after 14 days of illumination, the irradiation treatments did not increase ALP activity. The results of alizarin red staining showed that red LED promoted osteogenic differentiation of the PDLSCs. The real-time polymerase chain reaction (RT-PCR) results demonstrated that red LED upregulated the expression levels of osteogenic genes. Expression of the proteins BSP, OPN, OCN, and Runx2 in the irradiation groups was higher than that in the control group. Our results confirmed that low-energy red LED at 1, 3, and 5 J/cm
2
promotes proliferation and osteogenic differentiation of PDLSCs.</description><subject>Alizarin</subject><subject>Alkaline phosphatase</subject><subject>Biomedical materials</subject><subject>Cbfa-1 protein</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Dentistry</subject><subject>Differentiation (biology)</subject><subject>Gene expression</subject><subject>Illumination</subject><subject>Irradiation</subject><subject>Lasers</subject><subject>Ligaments</subject><subject>Light effects</subject><subject>Light emitting diodes</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal stem cells</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Periodontal ligament</subject><subject>Photonics</subject><subject>Polymerase chain reaction</subject><subject>Premolars</subject><subject>Proteins</subject><subject>Quantum Optics</subject><subject>Real time</subject><subject>Staining</subject><subject>Stem cells</subject><subject>Western blotting</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUGL1TAUhYMozpvRP-BCAm7cVO9NmqZdyjDqwIAbBXchbW_fy9AmzyQPcesvN7XPEVy4yuJ858uFw9gLhDcIoN8mBARZgcAKpNBthY_YDmupqgbqr4_ZDkTTVm0n8IJdpnQPgLpB-ZRdSKmxAwE79vM2Rjs6m13w_LvLBx5p5LPbH3JFi8vZ-T0fXRiJkz9YP1DixxhmN1HcStaPPKRMYU_eDYWdSkQ-n51h4keKRRB8tvNqtktJeWksfKB5Ts_Yk8nOiZ6f3yv25f3N5-uP1d2nD7fX7-6qQWqVK9n0SnWiHroJVI81SUUjAFBv0TY4tP2oetV2LZISoG2jJ5yIZK91A43W8oq93rzl_m8nStksLq0XWE_hlIxQgHWrWsCCvvoHvQ-n6Mt1hVJdp6BpV6HYqCGGlCJN5hjdYuMPg2DWhcy2kCkLmd8LmVX98qw-9QuND5U_kxRAbkAqkd9T_Pv3f7S_AOLYnbc</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Wu, Yan</creator><creator>Zhu, Tingting</creator><creator>Yang, Yaoyao</creator><creator>Gao, Hong</creator><creator>Shu, Chunxia</creator><creator>Chen, Qiang</creator><creator>Yang, Juan</creator><creator>Luo, Xiang</creator><creator>Wang, Yao</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8400-1665</orcidid></search><sort><creationdate>20210901</creationdate><title>Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells</title><author>Wu, Yan ; Zhu, Tingting ; Yang, Yaoyao ; Gao, Hong ; Shu, Chunxia ; Chen, Qiang ; Yang, Juan ; Luo, Xiang ; Wang, Yao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-36b55924c9f05b14e35ed000eba1a61c8bd5b58981e5207a67f1fee3b77606773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alizarin</topic><topic>Alkaline phosphatase</topic><topic>Biomedical materials</topic><topic>Cbfa-1 protein</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Dentistry</topic><topic>Differentiation (biology)</topic><topic>Gene expression</topic><topic>Illumination</topic><topic>Irradiation</topic><topic>Lasers</topic><topic>Ligaments</topic><topic>Light effects</topic><topic>Light emitting diodes</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal stem cells</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Periodontal ligament</topic><topic>Photonics</topic><topic>Polymerase chain reaction</topic><topic>Premolars</topic><topic>Proteins</topic><topic>Quantum Optics</topic><topic>Real time</topic><topic>Staining</topic><topic>Stem cells</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Yan</creatorcontrib><creatorcontrib>Zhu, Tingting</creatorcontrib><creatorcontrib>Yang, Yaoyao</creatorcontrib><creatorcontrib>Gao, Hong</creatorcontrib><creatorcontrib>Shu, Chunxia</creatorcontrib><creatorcontrib>Chen, Qiang</creatorcontrib><creatorcontrib>Yang, Juan</creatorcontrib><creatorcontrib>Luo, Xiang</creatorcontrib><creatorcontrib>Wang, Yao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Yan</au><au>Zhu, Tingting</au><au>Yang, Yaoyao</au><au>Gao, Hong</au><au>Shu, Chunxia</au><au>Chen, Qiang</au><au>Yang, Juan</au><au>Luo, Xiang</au><au>Wang, Yao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>36</volume><issue>7</issue><spage>1535</spage><epage>1543</epage><pages>1535-1543</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>This study aimed to evaluate the effects of low-energy red light-emitting diode (LED) irradiation on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). PDLSCs were derived from human periodontal ligament tissues of premolars and were irradiated with 0 (control group), 1, 3, or 5 J/cm
2
red LED in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) results. Osteoblast-associated proteins (Runx2, OCN, OPN, and BSP) were detected using western blotting. The results of the MTT assay indicated that PDLSCs in the irradiation groups exhibited a higher proliferation rate than those in the control group (
P
< 0.05). ALP results showed that after 7 days of illumination, only 5 J/cm
2
promoted the expression of ALP of PDLSCs. However, after 14 days of illumination, the irradiation treatments did not increase ALP activity. The results of alizarin red staining showed that red LED promoted osteogenic differentiation of the PDLSCs. The real-time polymerase chain reaction (RT-PCR) results demonstrated that red LED upregulated the expression levels of osteogenic genes. Expression of the proteins BSP, OPN, OCN, and Runx2 in the irradiation groups was higher than that in the control group. Our results confirmed that low-energy red LED at 1, 3, and 5 J/cm
2
promotes proliferation and osteogenic differentiation of PDLSCs.</abstract><cop>London</cop><pub>Springer London</pub><pmid>33719020</pmid><doi>10.1007/s10103-021-03278-1</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8400-1665</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-8921 |
| ispartof | Lasers in medical science, 2021-09, Vol.36 (7), p.1535-1543 |
| issn | 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2501485801 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Alizarin Alkaline phosphatase Biomedical materials Cbfa-1 protein Cell differentiation Cell proliferation Dentistry Differentiation (biology) Gene expression Illumination Irradiation Lasers Ligaments Light effects Light emitting diodes Medicine Medicine & Public Health Mesenchymal stem cells Optical Devices Optics Original Article Periodontal ligament Photonics Polymerase chain reaction Premolars Proteins Quantum Optics Real time Staining Stem cells Western blotting |
| title | Irradiation with red light-emitting diode enhances proliferation and osteogenic differentiation of periodontal ligament stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T14%3A11%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Irradiation%20with%20red%20light-emitting%20diode%20enhances%20proliferation%20and%20osteogenic%20differentiation%20of%20periodontal%20ligament%20stem%20cells&rft.jtitle=Lasers%20in%20medical%20science&rft.au=Wu,%20Yan&rft.date=2021-09-01&rft.volume=36&rft.issue=7&rft.spage=1535&rft.epage=1543&rft.pages=1535-1543&rft.issn=0268-8921&rft.eissn=1435-604X&rft_id=info:doi/10.1007/s10103-021-03278-1&rft_dat=%3Cproquest_cross%3E2559950687%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2559950687&rft_id=info:pmid/33719020&rfr_iscdi=true |