Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA
Cell-based therapy is a promising approach for the treatment of type-1 diabetes mellitus. Identifying stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support cell survival and their specifi...
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| Veröffentlicht in: | Life sciences (1973) 2021-06, Vol.274, p.119338-119338, Article 119338 |
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| container_title | Life sciences (1973) |
| container_volume | 274 |
| creator | Soltani, Adele Soleimani, Masoud Ghiass, Mohammad Adel Enderami, Seyed Ehsan Rabbani, Shahram Jafarian, Arefeh Allameh, Abdolamir |
| description | Cell-based therapy is a promising approach for the treatment of type-1 diabetes mellitus. Identifying stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support cell survival and their specific functions to control hyperglycemia conditions. Novel technologies using appropriate materials/fibers can improve cell transplantation.
In the present study, IPCs were differentiated from adipose-derived stem cells transduced with miR-375 and anti-miR-7. The cells' survival rate was also improved using a microfluidic system before their in vivo transplantation.
After adopting a stable, functional condition of the IPCs, the cells were used for in vivo grafting to diabetic mice, which resulted in a substantial drop in blood glucose during four weeks of grafting compared to the control group (p |
| doi_str_mv | 10.1016/j.lfs.2021.119338 |
| format | Article |
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In the present study, IPCs were differentiated from adipose-derived stem cells transduced with miR-375 and anti-miR-7. The cells' survival rate was also improved using a microfluidic system before their in vivo transplantation.
After adopting a stable, functional condition of the IPCs, the cells were used for in vivo grafting to diabetic mice, which resulted in a substantial drop in blood glucose during four weeks of grafting compared to the control group (p < 0.0001). The pattern of blood glucose levels in the mice receiving fiber entrapped IPCs, was similar to that of non-diabetic mice. Blood insulin was elevated in diabetic mice which received a transplant of fiber-entrapped-IPCs carrying miR-375 and anti-miR-7 after five weeks of transplantation compared to the diabetic mice (p < 0.014).
For the first time, this study showed that the two-component microfluidic system is useful for supporting the Collagen-Alginate fiber-entrapped IPCs and the miRNA-based cell therapy. Overall, our data show that the IPC encapsulation using a microfluidic system can support the cells in terms of morphology and biological function and their efficiency for controlling the hyperglycemia condition in diabetic mice.
[Display omitted]</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2021.119338</identifier><identifier>PMID: 33716064</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Alginates ; Alginic acid ; Animals ; Anti-miR-7 ; Blood ; Blood glucose ; Cell Differentiation ; Cell survival ; Cell therapy ; Collagen ; Cytology ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - genetics ; Diabetes Mellitus, Experimental - pathology ; Diabetes Mellitus, Experimental - therapy ; Encapsulation ; Glucose ; Grafting ; Hyperglycemia ; Insulin ; Insulin - metabolism ; Insulin-Secreting Cells - cytology ; Insulin-Secreting Cells - metabolism ; IPCs ; Male ; Mice ; Mice, Inbred BALB C ; Microfluidics ; Microfluidics - methods ; MicroRNAs - genetics ; miR-375 ; miRNA ; Morphology ; Stem cell transplantation ; Stem Cell Transplantation - methods ; Stem cells ; Stem Cells - cytology ; Survival ; Transplantation ; Transplants & implants</subject><ispartof>Life sciences (1973), 2021-06, Vol.274, p.119338-119338, Article 119338</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier Inc.</rights><rights>Copyright Elsevier BV Jun 1, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-cd478a18dc5cd124314a72438664574179139d8feb84eae1ce2052448f49c2193</citedby><cites>FETCH-LOGICAL-c381t-cd478a18dc5cd124314a72438664574179139d8feb84eae1ce2052448f49c2193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320521003234$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33716064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soltani, Adele</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Ghiass, Mohammad Adel</creatorcontrib><creatorcontrib>Enderami, Seyed Ehsan</creatorcontrib><creatorcontrib>Rabbani, Shahram</creatorcontrib><creatorcontrib>Jafarian, Arefeh</creatorcontrib><creatorcontrib>Allameh, Abdolamir</creatorcontrib><title>Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Cell-based therapy is a promising approach for the treatment of type-1 diabetes mellitus. Identifying stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support cell survival and their specific functions to control hyperglycemia conditions. Novel technologies using appropriate materials/fibers can improve cell transplantation.
In the present study, IPCs were differentiated from adipose-derived stem cells transduced with miR-375 and anti-miR-7. The cells' survival rate was also improved using a microfluidic system before their in vivo transplantation.
After adopting a stable, functional condition of the IPCs, the cells were used for in vivo grafting to diabetic mice, which resulted in a substantial drop in blood glucose during four weeks of grafting compared to the control group (p < 0.0001). The pattern of blood glucose levels in the mice receiving fiber entrapped IPCs, was similar to that of non-diabetic mice. Blood insulin was elevated in diabetic mice which received a transplant of fiber-entrapped-IPCs carrying miR-375 and anti-miR-7 after five weeks of transplantation compared to the diabetic mice (p < 0.014).
For the first time, this study showed that the two-component microfluidic system is useful for supporting the Collagen-Alginate fiber-entrapped IPCs and the miRNA-based cell therapy. Overall, our data show that the IPC encapsulation using a microfluidic system can support the cells in terms of morphology and biological function and their efficiency for controlling the hyperglycemia condition in diabetic mice.
[Display omitted]</description><subject>Alginates</subject><subject>Alginic acid</subject><subject>Animals</subject><subject>Anti-miR-7</subject><subject>Blood</subject><subject>Blood glucose</subject><subject>Cell Differentiation</subject><subject>Cell survival</subject><subject>Cell therapy</subject><subject>Collagen</subject><subject>Cytology</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - genetics</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetes Mellitus, Experimental - therapy</subject><subject>Encapsulation</subject><subject>Glucose</subject><subject>Grafting</subject><subject>Hyperglycemia</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin-Secreting Cells - cytology</subject><subject>Insulin-Secreting Cells - metabolism</subject><subject>IPCs</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microfluidics</subject><subject>Microfluidics - methods</subject><subject>MicroRNAs - genetics</subject><subject>miR-375</subject><subject>miRNA</subject><subject>Morphology</subject><subject>Stem cell transplantation</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Survival</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9q3DAQxkVoSLZJHqCXYuilF280lmzL9BRC_0FIoaRnoZXGrRZb3kpyyr5EnrljnPbQQ08jRr_5Zvg-xl4B3wKH5nq_Hfq0rXgFW4BOCHXCNqDaruSNgBdsw3klS1Hx-py9TGnPOa_rVpyxcyFaaHgjN-zpIaLJI4ZcTH3hvNlh9rYYvcVid1xqnPph9o6a6ZgyjqVJydPDFTmakA6DCdlkP4VFYAEKi8OQSofRPxLlQ5oHH8pDnNxsffi-_q_T1CHkl88_aNXX-5tLdtqbIeHVc71g3z68f7j9VN59-fj59uautEJBLq2TrTKgnK2tg0oKkKaloppG1q2EtgPROdXjTkk0CBbJhEpK1cvOVmTVBXu76tJVP2dMWY8-LXeZgNOcdFVzkEo0siX0zT_ofppjoOuIgo5LUcuGKFgp8iuliL0-RD-aeNTA9RKW3msKSy9h6TUsmnn9rDzvRnR_J_6kQ8C7FUCy4tFj1Ml6DGSZj2izdpP_j_xvk2GmOw</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Soltani, Adele</creator><creator>Soleimani, Masoud</creator><creator>Ghiass, Mohammad Adel</creator><creator>Enderami, Seyed Ehsan</creator><creator>Rabbani, Shahram</creator><creator>Jafarian, Arefeh</creator><creator>Allameh, Abdolamir</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20210601</creationdate><title>Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA</title><author>Soltani, Adele ; 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Identifying stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support cell survival and their specific functions to control hyperglycemia conditions. Novel technologies using appropriate materials/fibers can improve cell transplantation.
In the present study, IPCs were differentiated from adipose-derived stem cells transduced with miR-375 and anti-miR-7. The cells' survival rate was also improved using a microfluidic system before their in vivo transplantation.
After adopting a stable, functional condition of the IPCs, the cells were used for in vivo grafting to diabetic mice, which resulted in a substantial drop in blood glucose during four weeks of grafting compared to the control group (p < 0.0001). The pattern of blood glucose levels in the mice receiving fiber entrapped IPCs, was similar to that of non-diabetic mice. Blood insulin was elevated in diabetic mice which received a transplant of fiber-entrapped-IPCs carrying miR-375 and anti-miR-7 after five weeks of transplantation compared to the diabetic mice (p < 0.014).
For the first time, this study showed that the two-component microfluidic system is useful for supporting the Collagen-Alginate fiber-entrapped IPCs and the miRNA-based cell therapy. Overall, our data show that the IPC encapsulation using a microfluidic system can support the cells in terms of morphology and biological function and their efficiency for controlling the hyperglycemia condition in diabetic mice.
[Display omitted]</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>33716064</pmid><doi>10.1016/j.lfs.2021.119338</doi><tpages>1</tpages></addata></record> |
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| subjects | Alginates Alginic acid Animals Anti-miR-7 Blood Blood glucose Cell Differentiation Cell survival Cell therapy Collagen Cytology Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - genetics Diabetes Mellitus, Experimental - pathology Diabetes Mellitus, Experimental - therapy Encapsulation Glucose Grafting Hyperglycemia Insulin Insulin - metabolism Insulin-Secreting Cells - cytology Insulin-Secreting Cells - metabolism IPCs Male Mice Mice, Inbred BALB C Microfluidics Microfluidics - methods MicroRNAs - genetics miR-375 miRNA Morphology Stem cell transplantation Stem Cell Transplantation - methods Stem cells Stem Cells - cytology Survival Transplantation Transplants & implants |
| title | Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA |
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