Soluble Expression and Catalytic Properties of Codon-Optimized Recombinant Bromelain from MD2 Pineapple in Escherichia coli

Bromelain, a member of cysteine proteases, is found abundantly in pineapple ( Ananas comosus ), and it has a myriad of versatile applications. However, attempts to produce recombinant bromelain for commercialization purposes are challenging due to its expressibility and solubility. This study aims t...

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Veröffentlicht in:	The Protein Journal 2021-06, Vol.40 (3), p.406-418
Hauptverfasser:	Razali, Rafida, Budiman, Cahyo, Kamaruzaman, Khairul Azfar, Subbiah, Vijay Kumar
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Ananas - enzymology Ananas - genetics Animal Anatomy Biochemistry Bioorganic Chemistry Bromelains - biosynthesis Bromelains - chemistry Bromelains - genetics Bromelains - isolation & purification Catalysis Catalytic activity Chemistry Chemistry and Materials Science Circular dichroism Codon Commercialization Cysteine Cysteine proteinase Dichroism E coli Escherichia coli Ethylenediaminetetraacetic acid Ethylenediaminetetraacetic acids Fruit bromelain Gene Expression Genetic aspects Histology Metal ions Morphology Optimization Organic Chemistry pH effects Pineapples Plant Proteins - biosynthesis Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - isolation & purification Protease inhibitors Proteinase inhibitors Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Spectroscopy Thioredoxin
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