Aging and β3-adrenergic stimulation alter mitochondrial lipidome of adipose tissue
Mitochondrial abundance and thermogenic capacity are two imperative components that distinguish brown, beige and white adipose tissues. Most importantly, the lipid composition is vital for maintaining the quantity, quality and function of mitochondria. Therefore, we employed quantitative lipidomics...
Gespeichert in:
| Veröffentlicht in: | Biochimica et biophysica acta. Molecular and cell biology of lipids 2021-07, Vol.1866 (7), p.158922, Article 158922 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 7 |
| container_start_page | 158922 |
| container_title | Biochimica et biophysica acta. Molecular and cell biology of lipids |
| container_volume | 1866 |
| creator | Rajakumari, Sona Srivastava, Simran |
| description | Mitochondrial abundance and thermogenic capacity are two imperative components that distinguish brown, beige and white adipose tissues. Most importantly, the lipid composition is vital for maintaining the quantity, quality and function of mitochondria. Therefore, we employed quantitative lipidomics to probe the mitochondrial lipidome of adipose tissues. The mitochondrial lipidome reveals β3-adrenergic stimulation and aging drastically altered the levels of phosphatidylcholine (PC)/phosphatidylethanolamine (PE) ratio and acyl chain desaturation. Precisely, PC36:2 and PE38:4 levels correlate with the increased brown and beige fat activity in young mice. While aging increased lysoPC species in white adipose tissue (WAT) mitochondria, CL-316,243 administration reduced lysoPC species and increased lyso-PE18:1 and 18:2 content during WAT browning. Also, non-thermogenic mitochondria accumulate sphingomyelin (SM), phosphatidylserine (PS), phosphatidic acid (PA) and ether-linked PC (ePC). Similarly, enrichment of phosphatidylglycerol (PG) and cardiolipin (CL) levels are associated with thermogenic mitochondria. Also, our in vitro experiment supports that blocking the de novo sphingolipid synthesis pathway by myriocin, SPT1 inhibitor increased the thermogenic capacity and oxygen consumption rate in mature adipocytes. Overall, our study suggests mitochondria of brown, beige and white adipose tissues own a unique pattern of lipid molecular species and their levels are altered by aging and CL-316,243 administration.
[Display omitted]
•Mitochondria of brown, beige and white fat depots possess a distinct lipid profile.•Aging and β3-adrenergic stimulation remodel mitochondrial lipids.•The lipid profile distinguishes thermogenic and non-thermogenic mitochondria.•The thermogenic capacity of mitochondria associates with increased levels of LPE18:1 and 18:2, PC36:2 and PE38:4 species.•Accumulation of PA, PS and SM levels in mitochondria inhibits the uncoupled respiration. |
| doi_str_mv | 10.1016/j.bbalip.2021.158922 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2501257020</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1388198121000482</els_id><sourcerecordid>2501257020</sourcerecordid><originalsourceid>FETCH-LOGICAL-c277t-7e111d6e353a20f5aefa91aa9a20d681390a4581849c776a4f6189c59eaed6bb3</originalsourceid><addsrcrecordid>eNp9kM1u1TAQhS0EoqXwBgh5ySYXj32d2Bukqio_UiUWbdfWxJ5cfJXEFztB4rX6IDwTrlJYspoZzZk5Oh9jb0HsQED74bjrexzjaSeFhB1oY6V8xs7BdLaRLZjntVfGNGANnLFXpRyFAK2UfsnOlOrqTqlzdnt5iPOB4xz47wfVYMg0Uz5Ez8sSp3XEJaaZ47hQ5lNckv-e5pAjjrxax5Am4mngGOIpFeJLLGWl1-zFgGOhN0_1gt1_ur67-tLcfPv89erypvGy65amIwAILSmtUIpBIw1oAdHWKbQGlBW41wbM3vqua3E_1FTWa0tIoe17dcHeb39POf1YqSxuisXTOOJMaS1OagFSd0KKKt1vUp9TKZkGd8pxwvzLgXCPON3RbTjdI0634axn754c1n6i8O_oL78q-LgJqOb8GSm74iPNnkLM5BcXUvy_wx9J0YjZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2501257020</pqid></control><display><type>article</type><title>Aging and β3-adrenergic stimulation alter mitochondrial lipidome of adipose tissue</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Rajakumari, Sona ; Srivastava, Simran</creator><creatorcontrib>Rajakumari, Sona ; Srivastava, Simran</creatorcontrib><description>Mitochondrial abundance and thermogenic capacity are two imperative components that distinguish brown, beige and white adipose tissues. Most importantly, the lipid composition is vital for maintaining the quantity, quality and function of mitochondria. Therefore, we employed quantitative lipidomics to probe the mitochondrial lipidome of adipose tissues. The mitochondrial lipidome reveals β3-adrenergic stimulation and aging drastically altered the levels of phosphatidylcholine (PC)/phosphatidylethanolamine (PE) ratio and acyl chain desaturation. Precisely, PC36:2 and PE38:4 levels correlate with the increased brown and beige fat activity in young mice. While aging increased lysoPC species in white adipose tissue (WAT) mitochondria, CL-316,243 administration reduced lysoPC species and increased lyso-PE18:1 and 18:2 content during WAT browning. Also, non-thermogenic mitochondria accumulate sphingomyelin (SM), phosphatidylserine (PS), phosphatidic acid (PA) and ether-linked PC (ePC). Similarly, enrichment of phosphatidylglycerol (PG) and cardiolipin (CL) levels are associated with thermogenic mitochondria. Also, our in vitro experiment supports that blocking the de novo sphingolipid synthesis pathway by myriocin, SPT1 inhibitor increased the thermogenic capacity and oxygen consumption rate in mature adipocytes. Overall, our study suggests mitochondria of brown, beige and white adipose tissues own a unique pattern of lipid molecular species and their levels are altered by aging and CL-316,243 administration.
[Display omitted]
•Mitochondria of brown, beige and white fat depots possess a distinct lipid profile.•Aging and β3-adrenergic stimulation remodel mitochondrial lipids.•The lipid profile distinguishes thermogenic and non-thermogenic mitochondria.•The thermogenic capacity of mitochondria associates with increased levels of LPE18:1 and 18:2, PC36:2 and PE38:4 species.•Accumulation of PA, PS and SM levels in mitochondria inhibits the uncoupled respiration.</description><identifier>ISSN: 1388-1981</identifier><identifier>ISSN: 1879-2618</identifier><identifier>EISSN: 1879-2618</identifier><identifier>DOI: 10.1016/j.bbalip.2021.158922</identifier><identifier>PMID: 33713833</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acyl chain desaturation ; Adipose tissue ; Adipose Tissue - drug effects ; Adipose Tissue - metabolism ; Adipose Tissue, Beige - drug effects ; Adipose Tissue, Beige - metabolism ; Adipose Tissue, Brown - drug effects ; Adipose Tissue, Brown - metabolism ; Adipose Tissue, White - drug effects ; Adipose Tissue, White - metabolism ; Adrenergic beta-3 Receptor Agonists - pharmacology ; Aging ; Aging - metabolism ; Animals ; Dioxoles - pharmacology ; Lipid Metabolism - drug effects ; Lipidomics - methods ; Male ; Mice ; Mice, Inbred C57BL ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondrial lipidome ; Phosphatidylcholines - metabolism ; Phosphatidylethanolamines - metabolism ; Receptors, Adrenergic, beta-3 - metabolism ; Thermogenesis ; Thermogenesis - drug effects ; β3-adrenergic stimulation</subject><ispartof>Biochimica et biophysica acta. Molecular and cell biology of lipids, 2021-07, Vol.1866 (7), p.158922, Article 158922</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c277t-7e111d6e353a20f5aefa91aa9a20d681390a4581849c776a4f6189c59eaed6bb3</citedby><cites>FETCH-LOGICAL-c277t-7e111d6e353a20f5aefa91aa9a20d681390a4581849c776a4f6189c59eaed6bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1388198121000482$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33713833$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajakumari, Sona</creatorcontrib><creatorcontrib>Srivastava, Simran</creatorcontrib><title>Aging and β3-adrenergic stimulation alter mitochondrial lipidome of adipose tissue</title><title>Biochimica et biophysica acta. Molecular and cell biology of lipids</title><addtitle>Biochim Biophys Acta Mol Cell Biol Lipids</addtitle><description>Mitochondrial abundance and thermogenic capacity are two imperative components that distinguish brown, beige and white adipose tissues. Most importantly, the lipid composition is vital for maintaining the quantity, quality and function of mitochondria. Therefore, we employed quantitative lipidomics to probe the mitochondrial lipidome of adipose tissues. The mitochondrial lipidome reveals β3-adrenergic stimulation and aging drastically altered the levels of phosphatidylcholine (PC)/phosphatidylethanolamine (PE) ratio and acyl chain desaturation. Precisely, PC36:2 and PE38:4 levels correlate with the increased brown and beige fat activity in young mice. While aging increased lysoPC species in white adipose tissue (WAT) mitochondria, CL-316,243 administration reduced lysoPC species and increased lyso-PE18:1 and 18:2 content during WAT browning. Also, non-thermogenic mitochondria accumulate sphingomyelin (SM), phosphatidylserine (PS), phosphatidic acid (PA) and ether-linked PC (ePC). Similarly, enrichment of phosphatidylglycerol (PG) and cardiolipin (CL) levels are associated with thermogenic mitochondria. Also, our in vitro experiment supports that blocking the de novo sphingolipid synthesis pathway by myriocin, SPT1 inhibitor increased the thermogenic capacity and oxygen consumption rate in mature adipocytes. Overall, our study suggests mitochondria of brown, beige and white adipose tissues own a unique pattern of lipid molecular species and their levels are altered by aging and CL-316,243 administration.
[Display omitted]
•Mitochondria of brown, beige and white fat depots possess a distinct lipid profile.•Aging and β3-adrenergic stimulation remodel mitochondrial lipids.•The lipid profile distinguishes thermogenic and non-thermogenic mitochondria.•The thermogenic capacity of mitochondria associates with increased levels of LPE18:1 and 18:2, PC36:2 and PE38:4 species.•Accumulation of PA, PS and SM levels in mitochondria inhibits the uncoupled respiration.</description><subject>Acyl chain desaturation</subject><subject>Adipose tissue</subject><subject>Adipose Tissue - drug effects</subject><subject>Adipose Tissue - metabolism</subject><subject>Adipose Tissue, Beige - drug effects</subject><subject>Adipose Tissue, Beige - metabolism</subject><subject>Adipose Tissue, Brown - drug effects</subject><subject>Adipose Tissue, Brown - metabolism</subject><subject>Adipose Tissue, White - drug effects</subject><subject>Adipose Tissue, White - metabolism</subject><subject>Adrenergic beta-3 Receptor Agonists - pharmacology</subject><subject>Aging</subject><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Dioxoles - pharmacology</subject><subject>Lipid Metabolism - drug effects</subject><subject>Lipidomics - methods</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondrial lipidome</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phosphatidylethanolamines - metabolism</subject><subject>Receptors, Adrenergic, beta-3 - metabolism</subject><subject>Thermogenesis</subject><subject>Thermogenesis - drug effects</subject><subject>β3-adrenergic stimulation</subject><issn>1388-1981</issn><issn>1879-2618</issn><issn>1879-2618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1TAQhS0EoqXwBgh5ySYXj32d2Bukqio_UiUWbdfWxJ5cfJXEFztB4rX6IDwTrlJYspoZzZk5Oh9jb0HsQED74bjrexzjaSeFhB1oY6V8xs7BdLaRLZjntVfGNGANnLFXpRyFAK2UfsnOlOrqTqlzdnt5iPOB4xz47wfVYMg0Uz5Ez8sSp3XEJaaZ47hQ5lNckv-e5pAjjrxax5Am4mngGOIpFeJLLGWl1-zFgGOhN0_1gt1_ur67-tLcfPv89erypvGy65amIwAILSmtUIpBIw1oAdHWKbQGlBW41wbM3vqua3E_1FTWa0tIoe17dcHeb39POf1YqSxuisXTOOJMaS1OagFSd0KKKt1vUp9TKZkGd8pxwvzLgXCPON3RbTjdI0634axn754c1n6i8O_oL78q-LgJqOb8GSm74iPNnkLM5BcXUvy_wx9J0YjZ</recordid><startdate>202107</startdate><enddate>202107</enddate><creator>Rajakumari, Sona</creator><creator>Srivastava, Simran</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202107</creationdate><title>Aging and β3-adrenergic stimulation alter mitochondrial lipidome of adipose tissue</title><author>Rajakumari, Sona ; Srivastava, Simran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c277t-7e111d6e353a20f5aefa91aa9a20d681390a4581849c776a4f6189c59eaed6bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acyl chain desaturation</topic><topic>Adipose tissue</topic><topic>Adipose Tissue - drug effects</topic><topic>Adipose Tissue - metabolism</topic><topic>Adipose Tissue, Beige - drug effects</topic><topic>Adipose Tissue, Beige - metabolism</topic><topic>Adipose Tissue, Brown - drug effects</topic><topic>Adipose Tissue, Brown - metabolism</topic><topic>Adipose Tissue, White - drug effects</topic><topic>Adipose Tissue, White - metabolism</topic><topic>Adrenergic beta-3 Receptor Agonists - pharmacology</topic><topic>Aging</topic><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Dioxoles - pharmacology</topic><topic>Lipid Metabolism - drug effects</topic><topic>Lipidomics - methods</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondrial lipidome</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phosphatidylethanolamines - metabolism</topic><topic>Receptors, Adrenergic, beta-3 - metabolism</topic><topic>Thermogenesis</topic><topic>Thermogenesis - drug effects</topic><topic>β3-adrenergic stimulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajakumari, Sona</creatorcontrib><creatorcontrib>Srivastava, Simran</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimica et biophysica acta. Molecular and cell biology of lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajakumari, Sona</au><au>Srivastava, Simran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aging and β3-adrenergic stimulation alter mitochondrial lipidome of adipose tissue</atitle><jtitle>Biochimica et biophysica acta. Molecular and cell biology of lipids</jtitle><addtitle>Biochim Biophys Acta Mol Cell Biol Lipids</addtitle><date>2021-07</date><risdate>2021</risdate><volume>1866</volume><issue>7</issue><spage>158922</spage><pages>158922-</pages><artnum>158922</artnum><issn>1388-1981</issn><issn>1879-2618</issn><eissn>1879-2618</eissn><abstract>Mitochondrial abundance and thermogenic capacity are two imperative components that distinguish brown, beige and white adipose tissues. Most importantly, the lipid composition is vital for maintaining the quantity, quality and function of mitochondria. Therefore, we employed quantitative lipidomics to probe the mitochondrial lipidome of adipose tissues. The mitochondrial lipidome reveals β3-adrenergic stimulation and aging drastically altered the levels of phosphatidylcholine (PC)/phosphatidylethanolamine (PE) ratio and acyl chain desaturation. Precisely, PC36:2 and PE38:4 levels correlate with the increased brown and beige fat activity in young mice. While aging increased lysoPC species in white adipose tissue (WAT) mitochondria, CL-316,243 administration reduced lysoPC species and increased lyso-PE18:1 and 18:2 content during WAT browning. Also, non-thermogenic mitochondria accumulate sphingomyelin (SM), phosphatidylserine (PS), phosphatidic acid (PA) and ether-linked PC (ePC). Similarly, enrichment of phosphatidylglycerol (PG) and cardiolipin (CL) levels are associated with thermogenic mitochondria. Also, our in vitro experiment supports that blocking the de novo sphingolipid synthesis pathway by myriocin, SPT1 inhibitor increased the thermogenic capacity and oxygen consumption rate in mature adipocytes. Overall, our study suggests mitochondria of brown, beige and white adipose tissues own a unique pattern of lipid molecular species and their levels are altered by aging and CL-316,243 administration.
[Display omitted]
•Mitochondria of brown, beige and white fat depots possess a distinct lipid profile.•Aging and β3-adrenergic stimulation remodel mitochondrial lipids.•The lipid profile distinguishes thermogenic and non-thermogenic mitochondria.•The thermogenic capacity of mitochondria associates with increased levels of LPE18:1 and 18:2, PC36:2 and PE38:4 species.•Accumulation of PA, PS and SM levels in mitochondria inhibits the uncoupled respiration.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33713833</pmid><doi>10.1016/j.bbalip.2021.158922</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1388-1981 |
| ispartof | Biochimica et biophysica acta. Molecular and cell biology of lipids, 2021-07, Vol.1866 (7), p.158922, Article 158922 |
| issn | 1388-1981 1879-2618 1879-2618 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2501257020 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acyl chain desaturation Adipose tissue Adipose Tissue - drug effects Adipose Tissue - metabolism Adipose Tissue, Beige - drug effects Adipose Tissue, Beige - metabolism Adipose Tissue, Brown - drug effects Adipose Tissue, Brown - metabolism Adipose Tissue, White - drug effects Adipose Tissue, White - metabolism Adrenergic beta-3 Receptor Agonists - pharmacology Aging Aging - metabolism Animals Dioxoles - pharmacology Lipid Metabolism - drug effects Lipidomics - methods Male Mice Mice, Inbred C57BL Mitochondria - drug effects Mitochondria - metabolism Mitochondrial lipidome Phosphatidylcholines - metabolism Phosphatidylethanolamines - metabolism Receptors, Adrenergic, beta-3 - metabolism Thermogenesis Thermogenesis - drug effects β3-adrenergic stimulation |
| title | Aging and β3-adrenergic stimulation alter mitochondrial lipidome of adipose tissue |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T13%3A47%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aging%20and%20%CE%B23-adrenergic%20stimulation%20alter%20mitochondrial%20lipidome%20of%20adipose%20tissue&rft.jtitle=Biochimica%20et%20biophysica%20acta.%20Molecular%20and%20cell%20biology%20of%20lipids&rft.au=Rajakumari,%20Sona&rft.date=2021-07&rft.volume=1866&rft.issue=7&rft.spage=158922&rft.pages=158922-&rft.artnum=158922&rft.issn=1388-1981&rft.eissn=1879-2618&rft_id=info:doi/10.1016/j.bbalip.2021.158922&rft_dat=%3Cproquest_cross%3E2501257020%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2501257020&rft_id=info:pmid/33713833&rft_els_id=S1388198121000482&rfr_iscdi=true |