Shedding of Culturable Virus, Seroconversion, and 6-Month Follow-up Antibody Responses in the First 14 Confirmed Cases of Coronavirus Disease 2019 in the United States
Abstract We aimed to characterize presence of culturable virus in clinical specimens during acute illness, and antibody kinetics up to 6 months after symptom onset, among 14 early patients with coronavirus disease 2019 in the United States. We isolated viable severe acute respiratory syndrome corona...
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| Veröffentlicht in: | The Journal of infectious diseases 2021-09, Vol.224 (5), p.771-776 |
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| container_title | The Journal of infectious diseases |
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| creator | Killerby, Marie E Ata Ur Rasheed, Mohammed Tamin, Azaibi Harcourt, Jennifer L Abedi, Glen R Lu, Xiaoyan Kujawski, Stephanie Shah, Melisa M Kirking, Hannah L Gold, Jeremy A W Salvatore, Phillip P Coughlin, Melissa M Whitaker, Brett Tate, Jacqueline E Watson, John T Lindstrom, Stephen Hall, Aron J Fry, Alicia M Gerber, Susan I Midgley, Claire M Thornburg, Natalie J |
| description | Abstract
We aimed to characterize presence of culturable virus in clinical specimens during acute illness, and antibody kinetics up to 6 months after symptom onset, among 14 early patients with coronavirus disease 2019 in the United States. We isolated viable severe acute respiratory syndrome coronavirus 2 from real-time reverse-transcription polymerase chain reaction–positive respiratory specimens collected during days 0–8 after onset, but not after. All 13 patients with 2 or more serum specimens developed anti-spike antibodies; 12 developed detectable neutralizing antibodies. We did not isolate virus after detection of neutralizing antibodies. Eight participants provided serum at 6 months after onset; all retained detectable anti-spike immunoglobulin G, and half had detectable neutralizing antibodies. Two participants reported not feeling fully recovered at 6 months. |
| doi_str_mv | 10.1093/infdis/jiab125 |
| format | Article |
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We aimed to characterize presence of culturable virus in clinical specimens during acute illness, and antibody kinetics up to 6 months after symptom onset, among 14 early patients with coronavirus disease 2019 in the United States. We isolated viable severe acute respiratory syndrome coronavirus 2 from real-time reverse-transcription polymerase chain reaction–positive respiratory specimens collected during days 0–8 after onset, but not after. All 13 patients with 2 or more serum specimens developed anti-spike antibodies; 12 developed detectable neutralizing antibodies. We did not isolate virus after detection of neutralizing antibodies. Eight participants provided serum at 6 months after onset; all retained detectable anti-spike immunoglobulin G, and half had detectable neutralizing antibodies. Two participants reported not feeling fully recovered at 6 months.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/jiab125</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Antibodies ; Coronaviruses ; COVID-19 ; Immunoglobulin G ; Patients ; Polymerase chain reaction ; Seroconversion ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Viruses</subject><ispartof>The Journal of infectious diseases, 2021-09, Vol.224 (5), p.771-776</ispartof><rights>Published by Oxford University Press for the Infectious Diseases Society of America 2021. 2021</rights><rights>Published by Oxford University Press for the Infectious Diseases Society of America 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-696a65e51fa11c5f2435b08f85e1d39217db5fd901c00aabdc59223fc140e6b3</citedby><cites>FETCH-LOGICAL-c374t-696a65e51fa11c5f2435b08f85e1d39217db5fd901c00aabdc59223fc140e6b3</cites><orcidid>0000-0001-9726-8940</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Killerby, Marie E</creatorcontrib><creatorcontrib>Ata Ur Rasheed, Mohammed</creatorcontrib><creatorcontrib>Tamin, Azaibi</creatorcontrib><creatorcontrib>Harcourt, Jennifer L</creatorcontrib><creatorcontrib>Abedi, Glen R</creatorcontrib><creatorcontrib>Lu, Xiaoyan</creatorcontrib><creatorcontrib>Kujawski, Stephanie</creatorcontrib><creatorcontrib>Shah, Melisa M</creatorcontrib><creatorcontrib>Kirking, Hannah L</creatorcontrib><creatorcontrib>Gold, Jeremy A W</creatorcontrib><creatorcontrib>Salvatore, Phillip P</creatorcontrib><creatorcontrib>Coughlin, Melissa M</creatorcontrib><creatorcontrib>Whitaker, Brett</creatorcontrib><creatorcontrib>Tate, Jacqueline E</creatorcontrib><creatorcontrib>Watson, John T</creatorcontrib><creatorcontrib>Lindstrom, Stephen</creatorcontrib><creatorcontrib>Hall, Aron J</creatorcontrib><creatorcontrib>Fry, Alicia M</creatorcontrib><creatorcontrib>Gerber, Susan I</creatorcontrib><creatorcontrib>Midgley, Claire M</creatorcontrib><creatorcontrib>Thornburg, Natalie J</creatorcontrib><title>Shedding of Culturable Virus, Seroconversion, and 6-Month Follow-up Antibody Responses in the First 14 Confirmed Cases of Coronavirus Disease 2019 in the United States</title><title>The Journal of infectious diseases</title><description>Abstract
We aimed to characterize presence of culturable virus in clinical specimens during acute illness, and antibody kinetics up to 6 months after symptom onset, among 14 early patients with coronavirus disease 2019 in the United States. We isolated viable severe acute respiratory syndrome coronavirus 2 from real-time reverse-transcription polymerase chain reaction–positive respiratory specimens collected during days 0–8 after onset, but not after. All 13 patients with 2 or more serum specimens developed anti-spike antibodies; 12 developed detectable neutralizing antibodies. We did not isolate virus after detection of neutralizing antibodies. Eight participants provided serum at 6 months after onset; all retained detectable anti-spike immunoglobulin G, and half had detectable neutralizing antibodies. Two participants reported not feeling fully recovered at 6 months.</description><subject>Antibodies</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Immunoglobulin G</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Seroconversion</subject><subject>Severe acute respiratory syndrome</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv1DAQhS0EEkvhytkSF5Ca1mPHzvpYpWyLVITEFq6RE49Zr7J2sJ2i_iL-JlltuXDhMBpp5nvzRnqEvAV2AUyLSx-c9fly700PXD4jK5CiqZQC8ZysGOO8grXWL8mrnPeMsVqoZkV-b3dorQ8_aHS0nccyJ9OPSL_7NOdzusUUhxgeMGUfwzk1wVJVfY6h7OgmjmP8Vc0TvQrF99E-0q-YpxgyZuoDLTukG59yoVDTNgbn0wEtbc1xf3SLKQbzcDSi1z7jMqecgf6r_RZ8WfhtMQXza_LCmTHjm6d-Ru43H-_b2-ruy82n9uquGkRTl0ppZZRECc4ADNLxWsierd1aIlihOTS2l85qBgNjxvR2kJpz4QaoGapenJH3p7NTij9nzKU7-DzgOJqAcc4dl4yJhi-1oO_-QfdxTmF5ruMKGq0aWPOFujhRQ4o5J3TdlPzBpMcOWHeMrTvF1j3Ftgg-nARxnv7H_gFIIZvy</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>Killerby, Marie E</creator><creator>Ata Ur Rasheed, Mohammed</creator><creator>Tamin, Azaibi</creator><creator>Harcourt, Jennifer L</creator><creator>Abedi, Glen R</creator><creator>Lu, Xiaoyan</creator><creator>Kujawski, Stephanie</creator><creator>Shah, Melisa M</creator><creator>Kirking, Hannah L</creator><creator>Gold, Jeremy A W</creator><creator>Salvatore, Phillip P</creator><creator>Coughlin, Melissa M</creator><creator>Whitaker, Brett</creator><creator>Tate, Jacqueline E</creator><creator>Watson, John T</creator><creator>Lindstrom, Stephen</creator><creator>Hall, Aron J</creator><creator>Fry, Alicia M</creator><creator>Gerber, Susan I</creator><creator>Midgley, Claire M</creator><creator>Thornburg, Natalie J</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9726-8940</orcidid></search><sort><creationdate>20210901</creationdate><title>Shedding of Culturable Virus, Seroconversion, and 6-Month Follow-up Antibody Responses in the First 14 Confirmed Cases of Coronavirus Disease 2019 in the United States</title><author>Killerby, Marie E ; Ata Ur Rasheed, Mohammed ; Tamin, Azaibi ; Harcourt, Jennifer L ; Abedi, Glen R ; Lu, Xiaoyan ; Kujawski, Stephanie ; Shah, Melisa M ; Kirking, Hannah L ; Gold, Jeremy A W ; Salvatore, Phillip P ; Coughlin, Melissa M ; Whitaker, Brett ; Tate, Jacqueline E ; Watson, John T ; Lindstrom, Stephen ; Hall, Aron J ; Fry, Alicia M ; Gerber, Susan I ; Midgley, Claire M ; Thornburg, Natalie J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-696a65e51fa11c5f2435b08f85e1d39217db5fd901c00aabdc59223fc140e6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>Immunoglobulin G</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Seroconversion</topic><topic>Severe acute respiratory syndrome</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Killerby, Marie E</creatorcontrib><creatorcontrib>Ata Ur Rasheed, Mohammed</creatorcontrib><creatorcontrib>Tamin, Azaibi</creatorcontrib><creatorcontrib>Harcourt, Jennifer L</creatorcontrib><creatorcontrib>Abedi, Glen R</creatorcontrib><creatorcontrib>Lu, Xiaoyan</creatorcontrib><creatorcontrib>Kujawski, Stephanie</creatorcontrib><creatorcontrib>Shah, Melisa M</creatorcontrib><creatorcontrib>Kirking, Hannah L</creatorcontrib><creatorcontrib>Gold, Jeremy A W</creatorcontrib><creatorcontrib>Salvatore, Phillip P</creatorcontrib><creatorcontrib>Coughlin, Melissa M</creatorcontrib><creatorcontrib>Whitaker, Brett</creatorcontrib><creatorcontrib>Tate, Jacqueline E</creatorcontrib><creatorcontrib>Watson, John T</creatorcontrib><creatorcontrib>Lindstrom, Stephen</creatorcontrib><creatorcontrib>Hall, Aron J</creatorcontrib><creatorcontrib>Fry, Alicia M</creatorcontrib><creatorcontrib>Gerber, Susan I</creatorcontrib><creatorcontrib>Midgley, Claire M</creatorcontrib><creatorcontrib>Thornburg, Natalie J</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Killerby, Marie E</au><au>Ata Ur Rasheed, Mohammed</au><au>Tamin, Azaibi</au><au>Harcourt, Jennifer L</au><au>Abedi, Glen R</au><au>Lu, Xiaoyan</au><au>Kujawski, Stephanie</au><au>Shah, Melisa M</au><au>Kirking, Hannah L</au><au>Gold, Jeremy A W</au><au>Salvatore, Phillip P</au><au>Coughlin, Melissa M</au><au>Whitaker, Brett</au><au>Tate, Jacqueline E</au><au>Watson, John T</au><au>Lindstrom, Stephen</au><au>Hall, Aron J</au><au>Fry, Alicia M</au><au>Gerber, Susan I</au><au>Midgley, Claire M</au><au>Thornburg, Natalie J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shedding of Culturable Virus, Seroconversion, and 6-Month Follow-up Antibody Responses in the First 14 Confirmed Cases of Coronavirus Disease 2019 in the United States</atitle><jtitle>The Journal of infectious diseases</jtitle><date>2021-09-01</date><risdate>2021</risdate><volume>224</volume><issue>5</issue><spage>771</spage><epage>776</epage><pages>771-776</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><abstract>Abstract
We aimed to characterize presence of culturable virus in clinical specimens during acute illness, and antibody kinetics up to 6 months after symptom onset, among 14 early patients with coronavirus disease 2019 in the United States. We isolated viable severe acute respiratory syndrome coronavirus 2 from real-time reverse-transcription polymerase chain reaction–positive respiratory specimens collected during days 0–8 after onset, but not after. All 13 patients with 2 or more serum specimens developed anti-spike antibodies; 12 developed detectable neutralizing antibodies. We did not isolate virus after detection of neutralizing antibodies. Eight participants provided serum at 6 months after onset; all retained detectable anti-spike immunoglobulin G, and half had detectable neutralizing antibodies. Two participants reported not feeling fully recovered at 6 months.</abstract><cop>US</cop><pub>Oxford University Press</pub><doi>10.1093/infdis/jiab125</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-9726-8940</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of infectious diseases, 2021-09, Vol.224 (5), p.771-776 |
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| source | Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Antibodies Coronaviruses COVID-19 Immunoglobulin G Patients Polymerase chain reaction Seroconversion Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Viruses |
| title | Shedding of Culturable Virus, Seroconversion, and 6-Month Follow-up Antibody Responses in the First 14 Confirmed Cases of Coronavirus Disease 2019 in the United States |
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