Aptamer-based proximity labeling guides covalent RNA modification

Aptamer-based proximity labeling guides covalent RNA modification

We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA l...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2021-04, Vol.57 (28), p.348-3483
Hauptverfasser: Englert, Daniel, Matveeva, Regina, Sunbul, Murat, Wombacher, Richard, Jäschke, Andres
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Labeling
Rhodamine
Substrates
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container_issue 28
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container_title Chemical communications (Cambridge, England)
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creator Englert, Daniel
Matveeva, Regina
Sunbul, Murat
Wombacher, Richard
Jäschke, Andres
description We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA labeling in proof-of-principle experiments. An aptamer-based proximity-induced inverse electron demand Diels-Alder reaction for fluorescent modification of RNAs is described.
doi_str_mv 10.1039/d1cc00786f
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source Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Labeling
Rhodamine
Substrates
title Aptamer-based proximity labeling guides covalent RNA modification
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