Examination of FERMT1 expression in placental chorionic villi and its role in HTR8-SVneo cell invasion
Transmembrane integrin receptors mediate cell–extracellular matrix as well as cell–cell adhesion. As placental trophoblast cells undergo differentiation they display changes in integrin expression or switching, but the mechanism(s) of integrin activation that supports this differentiation is still u...
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| Veröffentlicht in: | Histochemistry and cell biology 2021-06, Vol.155 (6), p.669-681 |
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| description | Transmembrane integrin receptors mediate cell–extracellular matrix as well as cell–cell adhesion. As placental trophoblast cells undergo differentiation they display changes in integrin expression or switching, but the mechanism(s) of integrin activation that supports this differentiation is still unknown. The Fermitin family of adapter proteins (FERMT 1–3) are integrin activators that mediate integrin-mediated signaling. In this study, we examined the spatiotemporal pattern of expression of FERMT1 in human chorionic villi throughout gestation and its role in HTR8-SVneo substrate adhesion and invasion. Placental villous tissue was obtained from patients undergoing elective terminations at weeks 8–14, as well as from term deliveries at weeks 37–40 and analyzed by immunofluorescence. Additionally, HTR8-SVneo trophoblast cells were transfected with FERMT1-specific siRNA or non-targeting siRNA (control) and used in cell-substrate adhesion as well as invasion assays. FERMT1 was primarily localized to membrane-associated regions at the base or around the periphery of the villous cytotrophoblast and proximal as well as distal cell column trophoblast. FERMT1 was also localized to endothelial cells of blood vessels in chorionic villi. siRNA-mediated depletion of FERMT1 in HTR8-SVneo cells did not markedly alter HTR8-SVneo cell-substrate adhesion but did significantly decrease invasion (
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| doi_str_mv | 10.1007/s00418-021-01977-y |
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P
< 0.05) compared to control cells. These novel findings identify the presence of the integrin activator FERMT1 in trophoblast cells and that FERMT1 can regulate HTR8-SVneo cell invasion. FERMT1 may directly influence integrin activation and the subsequent integrin-mediated signaling and differentiation that underlies the acquisition of the invasive trophoblast phenotype in vivo.</description><identifier>ISSN: 0948-6143</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/s00418-021-01977-y</identifier><identifier>PMID: 33683437</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adapter proteins ; Adaptor proteins ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Blood vessels ; Cell adhesion ; Cell Biology ; Cell differentiation ; Developmental Biology ; Endothelial cells ; Extracellular matrix ; Gestation ; Immunofluorescence ; Original Paper ; Phenotypes ; Placenta ; siRNA</subject><ispartof>Histochemistry and cell biology, 2021-06, Vol.155 (6), p.669-681</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-1ea171bea9afecc90aa3da2fc281727f6a4be69b6994c659aa6fe651ef743d443</citedby><cites>FETCH-LOGICAL-c375t-1ea171bea9afecc90aa3da2fc281727f6a4be69b6994c659aa6fe651ef743d443</cites><orcidid>0000-0001-5721-6185</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00418-021-01977-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00418-021-01977-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33683437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawamura, E.</creatorcontrib><creatorcontrib>Hamilton, G. B.</creatorcontrib><creatorcontrib>Miskiewicz, E. I.</creatorcontrib><creatorcontrib>MacPhee, D. J.</creatorcontrib><title>Examination of FERMT1 expression in placental chorionic villi and its role in HTR8-SVneo cell invasion</title><title>Histochemistry and cell biology</title><addtitle>Histochem Cell Biol</addtitle><addtitle>Histochem Cell Biol</addtitle><description>Transmembrane integrin receptors mediate cell–extracellular matrix as well as cell–cell adhesion. As placental trophoblast cells undergo differentiation they display changes in integrin expression or switching, but the mechanism(s) of integrin activation that supports this differentiation is still unknown. The Fermitin family of adapter proteins (FERMT 1–3) are integrin activators that mediate integrin-mediated signaling. In this study, we examined the spatiotemporal pattern of expression of FERMT1 in human chorionic villi throughout gestation and its role in HTR8-SVneo substrate adhesion and invasion. Placental villous tissue was obtained from patients undergoing elective terminations at weeks 8–14, as well as from term deliveries at weeks 37–40 and analyzed by immunofluorescence. Additionally, HTR8-SVneo trophoblast cells were transfected with FERMT1-specific siRNA or non-targeting siRNA (control) and used in cell-substrate adhesion as well as invasion assays. FERMT1 was primarily localized to membrane-associated regions at the base or around the periphery of the villous cytotrophoblast and proximal as well as distal cell column trophoblast. FERMT1 was also localized to endothelial cells of blood vessels in chorionic villi. siRNA-mediated depletion of FERMT1 in HTR8-SVneo cells did not markedly alter HTR8-SVneo cell-substrate adhesion but did significantly decrease invasion (
P
< 0.05) compared to control cells. These novel findings identify the presence of the integrin activator FERMT1 in trophoblast cells and that FERMT1 can regulate HTR8-SVneo cell invasion. FERMT1 may directly influence integrin activation and the subsequent integrin-mediated signaling and differentiation that underlies the acquisition of the invasive trophoblast phenotype in vivo.</description><subject>Adapter proteins</subject><subject>Adaptor proteins</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood vessels</subject><subject>Cell adhesion</subject><subject>Cell Biology</subject><subject>Cell differentiation</subject><subject>Developmental Biology</subject><subject>Endothelial cells</subject><subject>Extracellular matrix</subject><subject>Gestation</subject><subject>Immunofluorescence</subject><subject>Original Paper</subject><subject>Phenotypes</subject><subject>Placenta</subject><subject>siRNA</subject><issn>0948-6143</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kUtPGzEUhS1UBOHxB1ggS910Y-rXjMfLCoWCRFUJAmJn3TjXrdFkJrUniPx7PIQWqQtWlo6_e-7RPYScCH4mODdfM-daNIxLwbiwxrDNDpkIrSQTwj58IhNudcPqouyTg5wfOReVlXKP7CtVN0orMyFh-gzL2MEQ-472gV5Mb37MBMXnVcKcRzF2dNWCx26AlvrffSpi9PQptm2k0C1oHDJNfYsjeTm7adjtfYc99di2RXqC0eWI7AZoMx6_vYfk7mI6O79k1z-_X51_u2ZemWpgAkEYMUewENB7ywHUAmTwshFGmlCDnmNt57W12teVBagD1pXAYLRaaK0OyZet7yr1f9aYB7eMeUwCJdI6O6mtLaeTvCno5__Qx36dupLOyUpZU_FKjYZyS_nU55wwuFWKS0gbJ7gbW3DbFlxpwb224DZl6PTNej1f4uLfyN-zF0BtgVy-ul-Y3nd_YPsCkomS_Q</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Kawamura, E.</creator><creator>Hamilton, G. 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B.</au><au>Miskiewicz, E. I.</au><au>MacPhee, D. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Examination of FERMT1 expression in placental chorionic villi and its role in HTR8-SVneo cell invasion</atitle><jtitle>Histochemistry and cell biology</jtitle><stitle>Histochem Cell Biol</stitle><addtitle>Histochem Cell Biol</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>155</volume><issue>6</issue><spage>669</spage><epage>681</epage><pages>669-681</pages><issn>0948-6143</issn><eissn>1432-119X</eissn><abstract>Transmembrane integrin receptors mediate cell–extracellular matrix as well as cell–cell adhesion. As placental trophoblast cells undergo differentiation they display changes in integrin expression or switching, but the mechanism(s) of integrin activation that supports this differentiation is still unknown. The Fermitin family of adapter proteins (FERMT 1–3) are integrin activators that mediate integrin-mediated signaling. In this study, we examined the spatiotemporal pattern of expression of FERMT1 in human chorionic villi throughout gestation and its role in HTR8-SVneo substrate adhesion and invasion. Placental villous tissue was obtained from patients undergoing elective terminations at weeks 8–14, as well as from term deliveries at weeks 37–40 and analyzed by immunofluorescence. Additionally, HTR8-SVneo trophoblast cells were transfected with FERMT1-specific siRNA or non-targeting siRNA (control) and used in cell-substrate adhesion as well as invasion assays. FERMT1 was primarily localized to membrane-associated regions at the base or around the periphery of the villous cytotrophoblast and proximal as well as distal cell column trophoblast. FERMT1 was also localized to endothelial cells of blood vessels in chorionic villi. siRNA-mediated depletion of FERMT1 in HTR8-SVneo cells did not markedly alter HTR8-SVneo cell-substrate adhesion but did significantly decrease invasion (
P
< 0.05) compared to control cells. These novel findings identify the presence of the integrin activator FERMT1 in trophoblast cells and that FERMT1 can regulate HTR8-SVneo cell invasion. FERMT1 may directly influence integrin activation and the subsequent integrin-mediated signaling and differentiation that underlies the acquisition of the invasive trophoblast phenotype in vivo.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33683437</pmid><doi>10.1007/s00418-021-01977-y</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-5721-6185</orcidid></addata></record> |
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| subjects | Adapter proteins Adaptor proteins Biochemistry Biomedical and Life Sciences Biomedicine Blood vessels Cell adhesion Cell Biology Cell differentiation Developmental Biology Endothelial cells Extracellular matrix Gestation Immunofluorescence Original Paper Phenotypes Placenta siRNA |
| title | Examination of FERMT1 expression in placental chorionic villi and its role in HTR8-SVneo cell invasion |
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