Fluorophore unmixing based on bleaching and recovery kinetics using MCR-ALS

Fluorescence microscopy is a key technology in the life sciences, though its performance is constrained by the number of labels that can be recorded. We propose to use the kinetics of fluorophore photodestruction and subsequent fluorescence recovery to distinguish multiple spectrally-overlapping emi...

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Veröffentlicht in:	Talanta (Oxford) 2021-05, Vol.226, p.122117-122117, Article 122117
Hauptverfasser:	Hugelier, S., Van den Eynde, R., Vandenberg, W., Dedecker, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Eukaryotic membrane labelling Fluorescence microscopy Image unmixing Multi-label imaging
Online-Zugang:	Volltext
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