In vitro human intestinal microbiota biotransformation of nobiletin using liquid chromatography–mass spectrometry analysis and background subtraction strategy
In this study, the in vitro biotransformation of nobiletin by human intestinal microbiota, which is a bioactive polymethoxyflavone widely presented in Citrus plants, has been investigated via utilizing an anaerobic incubation protocol. The incubation samples were detected using high‐performance liqu...
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Veröffentlicht in: | Journal of separation science 2021-05, Vol.44 (10), p.2046-2053 |
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creator | Lan, Hong‐Ci Li, Shang‐Zhen Li, Kai Liu, E.‐Hu |
description | In this study, the in vitro biotransformation of nobiletin by human intestinal microbiota, which is a bioactive polymethoxyflavone widely presented in Citrus plants, has been investigated via utilizing an anaerobic incubation protocol. The incubation samples were detected using high‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry. A background subtraction strategy incorporated in Microsoft Office was employed to eliminate the interferences in medium and feces. The parent and three metabolites sinensetin, 5‐hydroxy‐6,7,3′,4′‐tetramethoxyflavone, and 5‐demethylnobiletin were detected and identified based on the characteristics of their protonated molecules. The proposed metabolic pathway revealed that nobiletin went through phase I metabolism including demethylation and demethoxylation in human intestinal microbiota. The characterization of nobiletin metabolic profile transformed by human intestinal bacteria would be helpful for understanding its efficacy and action mechanism. |
doi_str_mv | 10.1002/jssc.202001150 |
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The incubation samples were detected using high‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry. A background subtraction strategy incorporated in Microsoft Office was employed to eliminate the interferences in medium and feces. The parent and three metabolites sinensetin, 5‐hydroxy‐6,7,3′,4′‐tetramethoxyflavone, and 5‐demethylnobiletin were detected and identified based on the characteristics of their protonated molecules. The proposed metabolic pathway revealed that nobiletin went through phase I metabolism including demethylation and demethoxylation in human intestinal microbiota. The characterization of nobiletin metabolic profile transformed by human intestinal bacteria would be helpful for understanding its efficacy and action mechanism.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.202001150</identifier><identifier>PMID: 33682313</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Biotransformation ; Chromatography ; human intestinal microbiota ; in vitro biotransformation ; Ions ; Liquid chromatography ; Mass spectrometry ; Metabolism ; Metabolites ; Microbiota ; nobiletin ; Quadrupoles ; Scientific imaging ; Spectroscopy ; Subtraction</subject><ispartof>Journal of separation science, 2021-05, Vol.44 (10), p.2046-2053</ispartof><rights>2021 Wiley‐VCH GmbH</rights><rights>2021 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4054-631fa3e812c41c8b2e242f4215d7a9f4176925cb0484a86d23335dc84d7eb4ec3</citedby><cites>FETCH-LOGICAL-c4054-631fa3e812c41c8b2e242f4215d7a9f4176925cb0484a86d23335dc84d7eb4ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.202001150$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.202001150$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33682313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lan, Hong‐Ci</creatorcontrib><creatorcontrib>Li, Shang‐Zhen</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Liu, E.‐Hu</creatorcontrib><title>In vitro human intestinal microbiota biotransformation of nobiletin using liquid chromatography–mass spectrometry analysis and background subtraction strategy</title><title>Journal of separation science</title><addtitle>J Sep Sci</addtitle><description>In this study, the in vitro biotransformation of nobiletin by human intestinal microbiota, which is a bioactive polymethoxyflavone widely presented in Citrus plants, has been investigated via utilizing an anaerobic incubation protocol. 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The characterization of nobiletin metabolic profile transformed by human intestinal bacteria would be helpful for understanding its efficacy and action mechanism.</description><subject>Biotransformation</subject><subject>Chromatography</subject><subject>human intestinal microbiota</subject><subject>in vitro biotransformation</subject><subject>Ions</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbiota</subject><subject>nobiletin</subject><subject>Quadrupoles</subject><subject>Scientific imaging</subject><subject>Spectroscopy</subject><subject>Subtraction</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkb1uFDEUhS1EREKgpUSWaNLsxr_zU6IVgaBIFIF65PF4dr3M2BvfcdB0eYe8AM_Gk3CXDVvQ0NhH8udzdO8h5A1nS86YuNwC2KVggjHONXtGznjB9aKWXD0_alackpcAW2TKqmYvyKmURSUkl2fk53Wg935KkW7yaAL1YXIw-WAGOnqbYuvjZOj-TCZAH9NoJh8DjT0N-Dg4ZGkGH9Z08HfZd9RuUkQorpPZbeZfD4-jAaCwcxZTRjelmRq0n8EDio62xn5fp5hRQm4xxv4JAFSTW8-vyElvBnCvn-5z8u3qw9fVp8XNl4_Xq_c3C6uYVotC8t5IV3FhFbdVK5xQoleC6640da94WdRC25apSpmq6ISUUne2Ul3pWuWsPCcXB99dincZd9CMHqwbBhNczNAIVVd1rbXgiL77B93GnHAmpLRkNS-xDKSWBwq3CJBc3-ySH02aG86afXfNvrvm2B1-ePtkm9vRdUf8b1kIqAPwA_c-_8eu-Xx7uyoFU_I3eiCqng</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Lan, Hong‐Ci</creator><creator>Li, Shang‐Zhen</creator><creator>Li, Kai</creator><creator>Liu, E.‐Hu</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>202105</creationdate><title>In vitro human intestinal microbiota biotransformation of nobiletin using liquid chromatography–mass spectrometry analysis and background subtraction strategy</title><author>Lan, Hong‐Ci ; Li, Shang‐Zhen ; Li, Kai ; Liu, E.‐Hu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4054-631fa3e812c41c8b2e242f4215d7a9f4176925cb0484a86d23335dc84d7eb4ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biotransformation</topic><topic>Chromatography</topic><topic>human intestinal microbiota</topic><topic>in vitro biotransformation</topic><topic>Ions</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Microbiota</topic><topic>nobiletin</topic><topic>Quadrupoles</topic><topic>Scientific imaging</topic><topic>Spectroscopy</topic><topic>Subtraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lan, Hong‐Ci</creatorcontrib><creatorcontrib>Li, Shang‐Zhen</creatorcontrib><creatorcontrib>Li, Kai</creatorcontrib><creatorcontrib>Liu, E.‐Hu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lan, Hong‐Ci</au><au>Li, Shang‐Zhen</au><au>Li, Kai</au><au>Liu, E.‐Hu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro human intestinal microbiota biotransformation of nobiletin using liquid chromatography–mass spectrometry analysis and background subtraction strategy</atitle><jtitle>Journal of separation science</jtitle><addtitle>J Sep Sci</addtitle><date>2021-05</date><risdate>2021</risdate><volume>44</volume><issue>10</issue><spage>2046</spage><epage>2053</epage><pages>2046-2053</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>In this study, the in vitro biotransformation of nobiletin by human intestinal microbiota, which is a bioactive polymethoxyflavone widely presented in Citrus plants, has been investigated via utilizing an anaerobic incubation protocol. The incubation samples were detected using high‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry. A background subtraction strategy incorporated in Microsoft Office was employed to eliminate the interferences in medium and feces. The parent and three metabolites sinensetin, 5‐hydroxy‐6,7,3′,4′‐tetramethoxyflavone, and 5‐demethylnobiletin were detected and identified based on the characteristics of their protonated molecules. The proposed metabolic pathway revealed that nobiletin went through phase I metabolism including demethylation and demethoxylation in human intestinal microbiota. The characterization of nobiletin metabolic profile transformed by human intestinal bacteria would be helpful for understanding its efficacy and action mechanism.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33682313</pmid><doi>10.1002/jssc.202001150</doi><tpages>8</tpages></addata></record> |
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subjects | Biotransformation Chromatography human intestinal microbiota in vitro biotransformation Ions Liquid chromatography Mass spectrometry Metabolism Metabolites Microbiota nobiletin Quadrupoles Scientific imaging Spectroscopy Subtraction |
title | In vitro human intestinal microbiota biotransformation of nobiletin using liquid chromatography–mass spectrometry analysis and background subtraction strategy |
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