Identification and a full genome analysis of novel camel hepatitis E virus strains obtained from Bactrian camels in Mongolia
•A high prevalence of HEV infection was found in domestic Bactrian camels in Mongolia.•Genotype 8 (G8) HEV strains were identified from two Bactrian camels.•Two camel HEV strains shared 97.7 % identity over the entire genome.•The strains differed from G8 HEV strains in China by 14 % over the entire...
Gespeichert in:
| Veröffentlicht in: | Virus research 2021-07, Vol.299, p.198355-198355, Article 198355 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 198355 |
|---|---|
| container_issue | |
| container_start_page | 198355 |
| container_title | Virus research |
| container_volume | 299 |
| creator | Nishizawa, Tsutomu Takahashi, Masaharu Tsatsralt-Od, Bira Nyamdavaa, Khurelbaatar Dulmaa, Nyamkhuu Osorjin, Byankhuu Tseren-Ochir, Erdene-Ochir Sharav, Tumenjargal Bayasgalan, Chimedtseren Sukhbaatar, Boldbaatar Nagashima, Shigeo Murata, Kazumoto Okamoto, Hiroaki |
| description | •A high prevalence of HEV infection was found in domestic Bactrian camels in Mongolia.•Genotype 8 (G8) HEV strains were identified from two Bactrian camels.•Two camel HEV strains shared 97.7 % identity over the entire genome.•The strains differed from G8 HEV strains in China by 14 % over the entire genome.•Circulation of region-dependent divergent G8 HEVs in Bactrian camels was suggested.
Hepatitis E virus (HEV) infects humans and a wide variety of other mammalian hosts. Recently, HEV strains belonging to genotype 8 (G8) within the Orthohepevirus A species of the Hepeviridae family, were identified in Bactrian camels (Camelus bactrianus) in China. The Bactrian camel (also known as the Mongolian camel) is native to the steppes of Central Asia. However, the HEV strains of Mongolian camels have not been examined. Among 200 serum samples from domestic Bactrian camels raised on 6 farms, in 6 soums in 3 provinces; 71 (35.5 %) were positive for anti-HEV IgG, with prevalence differing by farm (soum) (4.2−75.0 %); and 2 camels (1.0 %) that had been raised in Bogd, Bayankhongor Province, which had the highest seroprevalence among the six studied areas, were positive for HEV RNA. The two HEV strains (BcHEV-MNG140 and BcHEV-MNG146) obtained from the viremic camels in the present study shared 97.7 % nucleotide identity. They were closest to the reported G8 Chinese camel HEV strains but differed from them by 13.9−14.3 % over the entire genome, with a nucleotide difference of 24.0−26.5 % from the reported G1−G7 HEV strains. A phylogenetic tree indicated that the BcHEV-MNG140 and BcHEV-MNG146 strains were located upstream of a clade consisting of the Chinese camel HEV strains and formed a cluster with them, with a bootstrap value of 100 %, suggesting that they may represent a novel subtype within G8. These results indicate a high prevalence of HEV infection in Mongolian camels and suggest that the variability of camel HEV genomes is markedly high. |
| doi_str_mv | 10.1016/j.virusres.2021.198355 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2498507094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168170221000629</els_id><sourcerecordid>2498507094</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-f14ea528cdbfac93d37456eca0763c4a733a96955a030fd3472287dd393704293</originalsourceid><addsrcrecordid>eNqFkE1v1DAQhq0KRJfCX6h85JKtv2InN6Dql1TEBc6W1x63XiX2YjsrVeLH45KWKxeP5HnemdGD0DklW0qovNhvjyEvJUPZMsLolo4D7_sTtKGDYp0SI3uDNg0cOqoIO0XvS9kTQiRX8h065VxK1pgN-n3nINbggzU1pIhNdNhgv0wTfoCYZmg_ZnoqoeDkcUxHmLA1c3sf4dAitTWu8N9bcKnZhNjAXW0VHPY5zfirsTUHE9dYwSHibyk-pCmYD-itN1OBjy_1DP28vvpxedvdf7-5u_xy31nBRe08FWB6Nli388aO3HElegnWECW5FUZxbkY59r0hnHjHhWJsUM7xkSsi2MjP0Kd17iGnXwuUqudQLEyTiZCWopuKoSeKjKKhckVtTqXp9fqQw2zyk6ZEP5vXe_1qXj-b16v5Fjx_2bHsZnD_Yq-qG_B5BZoEOAbIutgA0YILGWzVLoX_7fgDydaZxA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2498507094</pqid></control><display><type>article</type><title>Identification and a full genome analysis of novel camel hepatitis E virus strains obtained from Bactrian camels in Mongolia</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Nishizawa, Tsutomu ; Takahashi, Masaharu ; Tsatsralt-Od, Bira ; Nyamdavaa, Khurelbaatar ; Dulmaa, Nyamkhuu ; Osorjin, Byankhuu ; Tseren-Ochir, Erdene-Ochir ; Sharav, Tumenjargal ; Bayasgalan, Chimedtseren ; Sukhbaatar, Boldbaatar ; Nagashima, Shigeo ; Murata, Kazumoto ; Okamoto, Hiroaki</creator><creatorcontrib>Nishizawa, Tsutomu ; Takahashi, Masaharu ; Tsatsralt-Od, Bira ; Nyamdavaa, Khurelbaatar ; Dulmaa, Nyamkhuu ; Osorjin, Byankhuu ; Tseren-Ochir, Erdene-Ochir ; Sharav, Tumenjargal ; Bayasgalan, Chimedtseren ; Sukhbaatar, Boldbaatar ; Nagashima, Shigeo ; Murata, Kazumoto ; Okamoto, Hiroaki</creatorcontrib><description>•A high prevalence of HEV infection was found in domestic Bactrian camels in Mongolia.•Genotype 8 (G8) HEV strains were identified from two Bactrian camels.•Two camel HEV strains shared 97.7 % identity over the entire genome.•The strains differed from G8 HEV strains in China by 14 % over the entire genome.•Circulation of region-dependent divergent G8 HEVs in Bactrian camels was suggested.
Hepatitis E virus (HEV) infects humans and a wide variety of other mammalian hosts. Recently, HEV strains belonging to genotype 8 (G8) within the Orthohepevirus A species of the Hepeviridae family, were identified in Bactrian camels (Camelus bactrianus) in China. The Bactrian camel (also known as the Mongolian camel) is native to the steppes of Central Asia. However, the HEV strains of Mongolian camels have not been examined. Among 200 serum samples from domestic Bactrian camels raised on 6 farms, in 6 soums in 3 provinces; 71 (35.5 %) were positive for anti-HEV IgG, with prevalence differing by farm (soum) (4.2−75.0 %); and 2 camels (1.0 %) that had been raised in Bogd, Bayankhongor Province, which had the highest seroprevalence among the six studied areas, were positive for HEV RNA. The two HEV strains (BcHEV-MNG140 and BcHEV-MNG146) obtained from the viremic camels in the present study shared 97.7 % nucleotide identity. They were closest to the reported G8 Chinese camel HEV strains but differed from them by 13.9−14.3 % over the entire genome, with a nucleotide difference of 24.0−26.5 % from the reported G1−G7 HEV strains. A phylogenetic tree indicated that the BcHEV-MNG140 and BcHEV-MNG146 strains were located upstream of a clade consisting of the Chinese camel HEV strains and formed a cluster with them, with a bootstrap value of 100 %, suggesting that they may represent a novel subtype within G8. These results indicate a high prevalence of HEV infection in Mongolian camels and suggest that the variability of camel HEV genomes is markedly high.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2021.198355</identifier><identifier>PMID: 33662492</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Bactrian camel ; Camelus - genetics ; Complete genome ; Hepatitis E - epidemiology ; Hepatitis E - veterinary ; Hepatitis E virus ; Hepatitis E virus - genetics ; Mongolia - epidemiology ; Nucleotides ; Phylogenetic analysis ; Phylogeny ; Seroepidemiologic Studies</subject><ispartof>Virus research, 2021-07, Vol.299, p.198355-198355, Article 198355</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-f14ea528cdbfac93d37456eca0763c4a733a96955a030fd3472287dd393704293</citedby><cites>FETCH-LOGICAL-c434t-f14ea528cdbfac93d37456eca0763c4a733a96955a030fd3472287dd393704293</cites><orcidid>0000-0003-2980-1875 ; 0000-0003-0827-0964 ; 0000-0002-3419-6297 ; 0000-0003-3237-7307</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2021.198355$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33662492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishizawa, Tsutomu</creatorcontrib><creatorcontrib>Takahashi, Masaharu</creatorcontrib><creatorcontrib>Tsatsralt-Od, Bira</creatorcontrib><creatorcontrib>Nyamdavaa, Khurelbaatar</creatorcontrib><creatorcontrib>Dulmaa, Nyamkhuu</creatorcontrib><creatorcontrib>Osorjin, Byankhuu</creatorcontrib><creatorcontrib>Tseren-Ochir, Erdene-Ochir</creatorcontrib><creatorcontrib>Sharav, Tumenjargal</creatorcontrib><creatorcontrib>Bayasgalan, Chimedtseren</creatorcontrib><creatorcontrib>Sukhbaatar, Boldbaatar</creatorcontrib><creatorcontrib>Nagashima, Shigeo</creatorcontrib><creatorcontrib>Murata, Kazumoto</creatorcontrib><creatorcontrib>Okamoto, Hiroaki</creatorcontrib><title>Identification and a full genome analysis of novel camel hepatitis E virus strains obtained from Bactrian camels in Mongolia</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>•A high prevalence of HEV infection was found in domestic Bactrian camels in Mongolia.•Genotype 8 (G8) HEV strains were identified from two Bactrian camels.•Two camel HEV strains shared 97.7 % identity over the entire genome.•The strains differed from G8 HEV strains in China by 14 % over the entire genome.•Circulation of region-dependent divergent G8 HEVs in Bactrian camels was suggested.
Hepatitis E virus (HEV) infects humans and a wide variety of other mammalian hosts. Recently, HEV strains belonging to genotype 8 (G8) within the Orthohepevirus A species of the Hepeviridae family, were identified in Bactrian camels (Camelus bactrianus) in China. The Bactrian camel (also known as the Mongolian camel) is native to the steppes of Central Asia. However, the HEV strains of Mongolian camels have not been examined. Among 200 serum samples from domestic Bactrian camels raised on 6 farms, in 6 soums in 3 provinces; 71 (35.5 %) were positive for anti-HEV IgG, with prevalence differing by farm (soum) (4.2−75.0 %); and 2 camels (1.0 %) that had been raised in Bogd, Bayankhongor Province, which had the highest seroprevalence among the six studied areas, were positive for HEV RNA. The two HEV strains (BcHEV-MNG140 and BcHEV-MNG146) obtained from the viremic camels in the present study shared 97.7 % nucleotide identity. They were closest to the reported G8 Chinese camel HEV strains but differed from them by 13.9−14.3 % over the entire genome, with a nucleotide difference of 24.0−26.5 % from the reported G1−G7 HEV strains. A phylogenetic tree indicated that the BcHEV-MNG140 and BcHEV-MNG146 strains were located upstream of a clade consisting of the Chinese camel HEV strains and formed a cluster with them, with a bootstrap value of 100 %, suggesting that they may represent a novel subtype within G8. These results indicate a high prevalence of HEV infection in Mongolian camels and suggest that the variability of camel HEV genomes is markedly high.</description><subject>Animals</subject><subject>Bactrian camel</subject><subject>Camelus - genetics</subject><subject>Complete genome</subject><subject>Hepatitis E - epidemiology</subject><subject>Hepatitis E - veterinary</subject><subject>Hepatitis E virus</subject><subject>Hepatitis E virus - genetics</subject><subject>Mongolia - epidemiology</subject><subject>Nucleotides</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Seroepidemiologic Studies</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhq0KRJfCX6h85JKtv2InN6Dql1TEBc6W1x63XiX2YjsrVeLH45KWKxeP5HnemdGD0DklW0qovNhvjyEvJUPZMsLolo4D7_sTtKGDYp0SI3uDNg0cOqoIO0XvS9kTQiRX8h065VxK1pgN-n3nINbggzU1pIhNdNhgv0wTfoCYZmg_ZnoqoeDkcUxHmLA1c3sf4dAitTWu8N9bcKnZhNjAXW0VHPY5zfirsTUHE9dYwSHibyk-pCmYD-itN1OBjy_1DP28vvpxedvdf7-5u_xy31nBRe08FWB6Nli388aO3HElegnWECW5FUZxbkY59r0hnHjHhWJsUM7xkSsi2MjP0Kd17iGnXwuUqudQLEyTiZCWopuKoSeKjKKhckVtTqXp9fqQw2zyk6ZEP5vXe_1qXj-b16v5Fjx_2bHsZnD_Yq-qG_B5BZoEOAbIutgA0YILGWzVLoX_7fgDydaZxA</recordid><startdate>20210702</startdate><enddate>20210702</enddate><creator>Nishizawa, Tsutomu</creator><creator>Takahashi, Masaharu</creator><creator>Tsatsralt-Od, Bira</creator><creator>Nyamdavaa, Khurelbaatar</creator><creator>Dulmaa, Nyamkhuu</creator><creator>Osorjin, Byankhuu</creator><creator>Tseren-Ochir, Erdene-Ochir</creator><creator>Sharav, Tumenjargal</creator><creator>Bayasgalan, Chimedtseren</creator><creator>Sukhbaatar, Boldbaatar</creator><creator>Nagashima, Shigeo</creator><creator>Murata, Kazumoto</creator><creator>Okamoto, Hiroaki</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2980-1875</orcidid><orcidid>https://orcid.org/0000-0003-0827-0964</orcidid><orcidid>https://orcid.org/0000-0002-3419-6297</orcidid><orcidid>https://orcid.org/0000-0003-3237-7307</orcidid></search><sort><creationdate>20210702</creationdate><title>Identification and a full genome analysis of novel camel hepatitis E virus strains obtained from Bactrian camels in Mongolia</title><author>Nishizawa, Tsutomu ; Takahashi, Masaharu ; Tsatsralt-Od, Bira ; Nyamdavaa, Khurelbaatar ; Dulmaa, Nyamkhuu ; Osorjin, Byankhuu ; Tseren-Ochir, Erdene-Ochir ; Sharav, Tumenjargal ; Bayasgalan, Chimedtseren ; Sukhbaatar, Boldbaatar ; Nagashima, Shigeo ; Murata, Kazumoto ; Okamoto, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-f14ea528cdbfac93d37456eca0763c4a733a96955a030fd3472287dd393704293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Bactrian camel</topic><topic>Camelus - genetics</topic><topic>Complete genome</topic><topic>Hepatitis E - epidemiology</topic><topic>Hepatitis E - veterinary</topic><topic>Hepatitis E virus</topic><topic>Hepatitis E virus - genetics</topic><topic>Mongolia - epidemiology</topic><topic>Nucleotides</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Seroepidemiologic Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishizawa, Tsutomu</creatorcontrib><creatorcontrib>Takahashi, Masaharu</creatorcontrib><creatorcontrib>Tsatsralt-Od, Bira</creatorcontrib><creatorcontrib>Nyamdavaa, Khurelbaatar</creatorcontrib><creatorcontrib>Dulmaa, Nyamkhuu</creatorcontrib><creatorcontrib>Osorjin, Byankhuu</creatorcontrib><creatorcontrib>Tseren-Ochir, Erdene-Ochir</creatorcontrib><creatorcontrib>Sharav, Tumenjargal</creatorcontrib><creatorcontrib>Bayasgalan, Chimedtseren</creatorcontrib><creatorcontrib>Sukhbaatar, Boldbaatar</creatorcontrib><creatorcontrib>Nagashima, Shigeo</creatorcontrib><creatorcontrib>Murata, Kazumoto</creatorcontrib><creatorcontrib>Okamoto, Hiroaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishizawa, Tsutomu</au><au>Takahashi, Masaharu</au><au>Tsatsralt-Od, Bira</au><au>Nyamdavaa, Khurelbaatar</au><au>Dulmaa, Nyamkhuu</au><au>Osorjin, Byankhuu</au><au>Tseren-Ochir, Erdene-Ochir</au><au>Sharav, Tumenjargal</au><au>Bayasgalan, Chimedtseren</au><au>Sukhbaatar, Boldbaatar</au><au>Nagashima, Shigeo</au><au>Murata, Kazumoto</au><au>Okamoto, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and a full genome analysis of novel camel hepatitis E virus strains obtained from Bactrian camels in Mongolia</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2021-07-02</date><risdate>2021</risdate><volume>299</volume><spage>198355</spage><epage>198355</epage><pages>198355-198355</pages><artnum>198355</artnum><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>•A high prevalence of HEV infection was found in domestic Bactrian camels in Mongolia.•Genotype 8 (G8) HEV strains were identified from two Bactrian camels.•Two camel HEV strains shared 97.7 % identity over the entire genome.•The strains differed from G8 HEV strains in China by 14 % over the entire genome.•Circulation of region-dependent divergent G8 HEVs in Bactrian camels was suggested.
Hepatitis E virus (HEV) infects humans and a wide variety of other mammalian hosts. Recently, HEV strains belonging to genotype 8 (G8) within the Orthohepevirus A species of the Hepeviridae family, were identified in Bactrian camels (Camelus bactrianus) in China. The Bactrian camel (also known as the Mongolian camel) is native to the steppes of Central Asia. However, the HEV strains of Mongolian camels have not been examined. Among 200 serum samples from domestic Bactrian camels raised on 6 farms, in 6 soums in 3 provinces; 71 (35.5 %) were positive for anti-HEV IgG, with prevalence differing by farm (soum) (4.2−75.0 %); and 2 camels (1.0 %) that had been raised in Bogd, Bayankhongor Province, which had the highest seroprevalence among the six studied areas, were positive for HEV RNA. The two HEV strains (BcHEV-MNG140 and BcHEV-MNG146) obtained from the viremic camels in the present study shared 97.7 % nucleotide identity. They were closest to the reported G8 Chinese camel HEV strains but differed from them by 13.9−14.3 % over the entire genome, with a nucleotide difference of 24.0−26.5 % from the reported G1−G7 HEV strains. A phylogenetic tree indicated that the BcHEV-MNG140 and BcHEV-MNG146 strains were located upstream of a clade consisting of the Chinese camel HEV strains and formed a cluster with them, with a bootstrap value of 100 %, suggesting that they may represent a novel subtype within G8. These results indicate a high prevalence of HEV infection in Mongolian camels and suggest that the variability of camel HEV genomes is markedly high.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33662492</pmid><doi>10.1016/j.virusres.2021.198355</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2980-1875</orcidid><orcidid>https://orcid.org/0000-0003-0827-0964</orcidid><orcidid>https://orcid.org/0000-0002-3419-6297</orcidid><orcidid>https://orcid.org/0000-0003-3237-7307</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1702 |
| ispartof | Virus research, 2021-07, Vol.299, p.198355-198355, Article 198355 |
| issn | 0168-1702 1872-7492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2498507094 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Bactrian camel Camelus - genetics Complete genome Hepatitis E - epidemiology Hepatitis E - veterinary Hepatitis E virus Hepatitis E virus - genetics Mongolia - epidemiology Nucleotides Phylogenetic analysis Phylogeny Seroepidemiologic Studies |
| title | Identification and a full genome analysis of novel camel hepatitis E virus strains obtained from Bactrian camels in Mongolia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T20%3A50%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20a%20full%20genome%20analysis%20of%20novel%20camel%20hepatitis%20E%20virus%20strains%20obtained%20from%20Bactrian%20camels%20in%20Mongolia&rft.jtitle=Virus%20research&rft.au=Nishizawa,%20Tsutomu&rft.date=2021-07-02&rft.volume=299&rft.spage=198355&rft.epage=198355&rft.pages=198355-198355&rft.artnum=198355&rft.issn=0168-1702&rft.eissn=1872-7492&rft_id=info:doi/10.1016/j.virusres.2021.198355&rft_dat=%3Cproquest_cross%3E2498507094%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2498507094&rft_id=info:pmid/33662492&rft_els_id=S0168170221000629&rfr_iscdi=true |