Changes in microglia number and Iba1 expression level in the prefrontal cortex of type 1 diabetic mice
The aim of the present study was to observe the activation of microglia in the prefrontal cortex of type 1 diabetes mellitus (T1DM) mice, and the expression of the marker genes of the disease-associated microglia (DAM) associated with neurodegenerative diseases. Sixty healthy adult male C57BL/6J mic...
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| Veröffentlicht in: | Sheng li hsüeh pao 2021-02, Vol.73 (1), p.10-16 |
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| description | The aim of the present study was to observe the activation of microglia in the prefrontal cortex of type 1 diabetes mellitus (T1DM) mice, and the expression of the marker genes of the disease-associated microglia (DAM) associated with neurodegenerative diseases. Sixty healthy adult male C57BL/6J mice were randomly divided into two groups, normal control (CON) group and T1DM group. Streptozocin (STZ) was injected intraperitoneally to induce T1DM mice. The spatial learning and memory function of mice was detected by Morris water maze at the 8th week after the successful model establishment. The number and activation of microglia in the prefrontal cortex of mice were detected by immunofluorescence staining and Western blot. Changes in the mRNA level of several DAM molecular markers were detected by RT-FQ-PCR. The results showed that, compared with CON mice, the fasting blood glucose of T1DM mice increased significantly, while the body weight of T1DM mice decreased remarkably (P < 0.05). The escape latency of wat |
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Sixty healthy adult male C57BL/6J mice were randomly divided into two groups, normal control (CON) group and T1DM group. Streptozocin (STZ) was injected intraperitoneally to induce T1DM mice. The spatial learning and memory function of mice was detected by Morris water maze at the 8th week after the successful model establishment. The number and activation of microglia in the prefrontal cortex of mice were detected by immunofluorescence staining and Western blot. Changes in the mRNA level of several DAM molecular markers were detected by RT-FQ-PCR. The results showed that, compared with CON mice, the fasting blood glucose of T1DM mice increased significantly, while the body weight of T1DM mice decreased remarkably (P < 0.05). The escape latency of wat</description><identifier>ISSN: 0371-0874</identifier><identifier>PMID: 33665655</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Diabetes Mellitus, Experimental ; Diabetes Mellitus, Type 1 ; Hippocampus ; Male ; Mice ; Mice, Inbred C57BL ; Microglia ; Prefrontal Cortex</subject><ispartof>Sheng li hsüeh pao, 2021-02, Vol.73 (1), p.10-16</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33665655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Ru-Yue</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Han, Song</creatorcontrib><creatorcontrib>Liu, Yue</creatorcontrib><creatorcontrib>Li, Jun-Fa</creatorcontrib><creatorcontrib>Zhao, Li</creatorcontrib><title>Changes in microglia number and Iba1 expression level in the prefrontal cortex of type 1 diabetic mice</title><title>Sheng li hsüeh pao</title><addtitle>Sheng Li Xue Bao</addtitle><description>The aim of the present study was to observe the activation of microglia in the prefrontal cortex of type 1 diabetes mellitus (T1DM) mice, and the expression of the marker genes of the disease-associated microglia (DAM) associated with neurodegenerative diseases. Sixty healthy adult male C57BL/6J mice were randomly divided into two groups, normal control (CON) group and T1DM group. Streptozocin (STZ) was injected intraperitoneally to induce T1DM mice. The spatial learning and memory function of mice was detected by Morris water maze at the 8th week after the successful model establishment. The number and activation of microglia in the prefrontal cortex of mice were detected by immunofluorescence staining and Western blot. Changes in the mRNA level of several DAM molecular markers were detected by RT-FQ-PCR. The results showed that, compared with CON mice, the fasting blood glucose of T1DM mice increased significantly, while the body weight of T1DM mice decreased remarkably (P < 0.05). The escape latency of wat</description><subject>Animals</subject><subject>Diabetes Mellitus, Experimental</subject><subject>Diabetes Mellitus, Type 1</subject><subject>Hippocampus</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microglia</subject><subject>Prefrontal Cortex</subject><issn>0371-0874</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtqwzAQRb1oaUKaXyhadmOQLMmSliX0ESh0k73RY5wIZMmV7JL8fR2awsDAcDjcO3fVGlNBaiwFW1XbUrzBnFDBmBQP1YrStuUt5-uq3510PEJBPqLB25yOwWsU58FARjo6tDeaIDiPGRZJiijAD4QrPZ0ALdc-pzjpgGzKE5xR6tF0GQER5Lw2MHl71cJjdd_rUGB725vq8PZ62H3Un1_v-93LZz0uceqmBcqwU5gQaXpBVQPKaCkkV8xRToRoOdXLSEON04phYZ0lSykraKMx3VTPf9oxp-8ZytQNvlgIQUdIc-kapiRTjDK-oE83dDYDuG7MftD50v2_hv4CM9Jexg</recordid><startdate>20210225</startdate><enddate>20210225</enddate><creator>Wang, Ru-Yue</creator><creator>Wang, Yue</creator><creator>Han, Song</creator><creator>Liu, Yue</creator><creator>Li, Jun-Fa</creator><creator>Zhao, Li</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20210225</creationdate><title>Changes in microglia number and Iba1 expression level in the prefrontal cortex of type 1 diabetic mice</title><author>Wang, Ru-Yue ; Wang, Yue ; Han, Song ; Liu, Yue ; Li, Jun-Fa ; Zhao, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p565-26e340d90118bf7392e9ba878594d35177653a53a8b3bda9407cdc1448c732a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Diabetes Mellitus, Experimental</topic><topic>Diabetes Mellitus, Type 1</topic><topic>Hippocampus</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microglia</topic><topic>Prefrontal Cortex</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ru-Yue</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Han, Song</creatorcontrib><creatorcontrib>Liu, Yue</creatorcontrib><creatorcontrib>Li, Jun-Fa</creatorcontrib><creatorcontrib>Zhao, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Sheng li hsüeh pao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ru-Yue</au><au>Wang, Yue</au><au>Han, Song</au><au>Liu, Yue</au><au>Li, Jun-Fa</au><au>Zhao, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in microglia number and Iba1 expression level in the prefrontal cortex of type 1 diabetic mice</atitle><jtitle>Sheng li hsüeh pao</jtitle><addtitle>Sheng Li Xue Bao</addtitle><date>2021-02-25</date><risdate>2021</risdate><volume>73</volume><issue>1</issue><spage>10</spage><epage>16</epage><pages>10-16</pages><issn>0371-0874</issn><abstract>The aim of the present study was to observe the activation of microglia in the prefrontal cortex of type 1 diabetes mellitus (T1DM) mice, and the expression of the marker genes of the disease-associated microglia (DAM) associated with neurodegenerative diseases. Sixty healthy adult male C57BL/6J mice were randomly divided into two groups, normal control (CON) group and T1DM group. Streptozocin (STZ) was injected intraperitoneally to induce T1DM mice. The spatial learning and memory function of mice was detected by Morris water maze at the 8th week after the successful model establishment. The number and activation of microglia in the prefrontal cortex of mice were detected by immunofluorescence staining and Western blot. Changes in the mRNA level of several DAM molecular markers were detected by RT-FQ-PCR. The results showed that, compared with CON mice, the fasting blood glucose of T1DM mice increased significantly, while the body weight of T1DM mice decreased remarkably (P < 0.05). The escape latency of wat</abstract><cop>China</cop><pmid>33665655</pmid><tpages>7</tpages></addata></record> |
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| language | chi |
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| subjects | Animals Diabetes Mellitus, Experimental Diabetes Mellitus, Type 1 Hippocampus Male Mice Mice, Inbred C57BL Microglia Prefrontal Cortex |
| title | Changes in microglia number and Iba1 expression level in the prefrontal cortex of type 1 diabetic mice |
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