Electrochemical determination of hantavirus using gold nanoparticle-modified graphene as an electrode material and Cu-based metal-organic framework assisted signal generation
An electrochemical biosensor was prepared for nucleic acid-based hantavirus detection using a Cu-based metal-organic framework (CuMOF) as a signal tag. The CuMOF was synthesized by the solvothermal method and then covalently bonded with signal DNA (sDNA) probes. The Au nanoparticles and reduced grap...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2021-03, Vol.188 (4), p.112-112, Article 112 |
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| container_title | Mikrochimica acta (1966) |
| container_volume | 188 |
| creator | Yiwei, Xu Yahui, Li Weilong, Tan Jiyong, Shi Xiaobo, Zou Wen, Zhang Xinai, Zhang Yanxiao, Li Changqiang, Zhu Lele, Ai Hong, Li Tingting, Shen |
| description | An electrochemical biosensor was prepared for nucleic acid-based hantavirus detection using a Cu-based metal-organic framework (CuMOF) as a signal tag. The CuMOF was synthesized by the solvothermal method and then covalently bonded with signal DNA (sDNA) probes. The Au nanoparticles and reduced graphene oxide composite were deposited on the electrode surface by electroreduction as support substrate and was then functionalized with capture DNA (cDNA) probes by self-assembly. Through the complementary base pairing, the target DNA (tDNA) fragment of hantavirus hybridized with the cDNA and the sDNA in a sandwich-type format. The tDNA was detected according to the current signal of the CuMOF catalyzed reaction using
o
-phenylenediamine as redox substrate. The peak current of the biosensor at − 0.55 V increased linearly in proportion to the logarithmic value of the tDNA concentration from 10
−15
to 10
−9
mol/L, with a detection limit of 0.74 × 10
−15
mol/L. Moreover, the proposed biosensor was successfully applied to detect hantavirus and was able to distinguish hantavirus from other arboviruses.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-021-04769-2 |
| format | Article |
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o
-phenylenediamine as redox substrate. The peak current of the biosensor at − 0.55 V increased linearly in proportion to the logarithmic value of the tDNA concentration from 10
−15
to 10
−9
mol/L, with a detection limit of 0.74 × 10
−15
mol/L. Moreover, the proposed biosensor was successfully applied to detect hantavirus and was able to distinguish hantavirus from other arboviruses.
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o
-phenylenediamine as redox substrate. The peak current of the biosensor at − 0.55 V increased linearly in proportion to the logarithmic value of the tDNA concentration from 10
−15
to 10
−9
mol/L, with a detection limit of 0.74 × 10
−15
mol/L. Moreover, the proposed biosensor was successfully applied to detect hantavirus and was able to distinguish hantavirus from other arboviruses.
Graphical abstract</description><subject>Analytical Chemistry</subject><subject>Biosensors</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Copper</subject><subject>Deoxyribonucleic acid</subject><subject>Detectors</subject><subject>DNA</subject><subject>Electric properties</subject><subject>Electrode materials</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Gold</subject><subject>Graphene</subject><subject>Graphite</subject><subject>Hantavirus</subject><subject>Metal-organic frameworks</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nucleic acids</subject><subject>Original Paper</subject><subject>Phenylenediamine</subject><subject>Self-assembly</subject><subject>Signal generation</subject><subject>Substrates</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kcuKFDEUhgtRnHb0BVxIwI2bjLmnejk04wUG3Oi6OFU5qc5YlbRJlYMv5TOa7h4VXEgWgZPv_zjkb5qXnF1xxuzbwphhijLBKVPWbKl41Gy4koZqZuXjZsOYMFQaKy6aZ6XcMcatEeppcyHrUCslNs3PmwmHJadhj3MYYCIOF8xziLCEFEnyZA9xge8hr4WsJcSRjGlyJEJMB8hLGCakc3LBB3RkzHDYY0QChUAkeHY7JDNUa6h6iI7sVtpDqfiMC0w05RFiGIjPMON9yl9ruoSyVKCEMdbQWJX5tNDz5omHqeCLh_uy-fLu5vPuA7399P7j7vqWDoqrhXrBWzsY0CicauW2d23f96rlVhsAwbj2kmnvrXPCadWbXrZStcZaEEZLLy-bN2fvIadvK5alm0MZcJogYlpLJ9S2reJWsIq-_ge9S2uua58oa4ViVlTq6kyNMGEXok9LhqEed_z3FNGHOr-2XDOtt-IYEOfAkFMpGX13yGGG_KPjrDvW353r72r93an-7hh69bDL2s_o_kR-910BeQZKfYoj5r_L_kf7C95avgY</recordid><startdate>20210306</startdate><enddate>20210306</enddate><creator>Yiwei, Xu</creator><creator>Yahui, Li</creator><creator>Weilong, Tan</creator><creator>Jiyong, Shi</creator><creator>Xiaobo, Zou</creator><creator>Wen, Zhang</creator><creator>Xinai, Zhang</creator><creator>Yanxiao, Li</creator><creator>Changqiang, Zhu</creator><creator>Lele, Ai</creator><creator>Hong, Li</creator><creator>Tingting, Shen</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5719-7025</orcidid></search><sort><creationdate>20210306</creationdate><title>Electrochemical determination of hantavirus using gold nanoparticle-modified graphene as an electrode material and Cu-based metal-organic framework assisted signal generation</title><author>Yiwei, Xu ; Yahui, Li ; Weilong, Tan ; Jiyong, Shi ; Xiaobo, Zou ; Wen, Zhang ; Xinai, Zhang ; Yanxiao, Li ; Changqiang, Zhu ; Lele, Ai ; Hong, Li ; Tingting, Shen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-f2187c6a5e2d4839bd8bbb481756aa2015f305ff7dd2d54b6b38348677a2653f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical Chemistry</topic><topic>Biosensors</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Copper</topic><topic>Deoxyribonucleic acid</topic><topic>Detectors</topic><topic>DNA</topic><topic>Electric properties</topic><topic>Electrode materials</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Gold</topic><topic>Graphene</topic><topic>Graphite</topic><topic>Hantavirus</topic><topic>Metal-organic frameworks</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nucleic acids</topic><topic>Original Paper</topic><topic>Phenylenediamine</topic><topic>Self-assembly</topic><topic>Signal generation</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yiwei, Xu</creatorcontrib><creatorcontrib>Yahui, Li</creatorcontrib><creatorcontrib>Weilong, Tan</creatorcontrib><creatorcontrib>Jiyong, Shi</creatorcontrib><creatorcontrib>Xiaobo, Zou</creatorcontrib><creatorcontrib>Wen, Zhang</creatorcontrib><creatorcontrib>Xinai, Zhang</creatorcontrib><creatorcontrib>Yanxiao, Li</creatorcontrib><creatorcontrib>Changqiang, Zhu</creatorcontrib><creatorcontrib>Lele, Ai</creatorcontrib><creatorcontrib>Hong, Li</creatorcontrib><creatorcontrib>Tingting, Shen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yiwei, Xu</au><au>Yahui, Li</au><au>Weilong, Tan</au><au>Jiyong, Shi</au><au>Xiaobo, Zou</au><au>Wen, Zhang</au><au>Xinai, Zhang</au><au>Yanxiao, Li</au><au>Changqiang, Zhu</au><au>Lele, Ai</au><au>Hong, Li</au><au>Tingting, Shen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical determination of hantavirus using gold nanoparticle-modified graphene as an electrode material and Cu-based metal-organic framework assisted signal generation</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2021-03-06</date><risdate>2021</risdate><volume>188</volume><issue>4</issue><spage>112</spage><epage>112</epage><pages>112-112</pages><artnum>112</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>An electrochemical biosensor was prepared for nucleic acid-based hantavirus detection using a Cu-based metal-organic framework (CuMOF) as a signal tag. The CuMOF was synthesized by the solvothermal method and then covalently bonded with signal DNA (sDNA) probes. The Au nanoparticles and reduced graphene oxide composite were deposited on the electrode surface by electroreduction as support substrate and was then functionalized with capture DNA (cDNA) probes by self-assembly. Through the complementary base pairing, the target DNA (tDNA) fragment of hantavirus hybridized with the cDNA and the sDNA in a sandwich-type format. The tDNA was detected according to the current signal of the CuMOF catalyzed reaction using
o
-phenylenediamine as redox substrate. The peak current of the biosensor at − 0.55 V increased linearly in proportion to the logarithmic value of the tDNA concentration from 10
−15
to 10
−9
mol/L, with a detection limit of 0.74 × 10
−15
mol/L. Moreover, the proposed biosensor was successfully applied to detect hantavirus and was able to distinguish hantavirus from other arboviruses.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>33675442</pmid><doi>10.1007/s00604-021-04769-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5719-7025</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-3672 |
| ispartof | Mikrochimica acta (1966), 2021-03, Vol.188 (4), p.112-112, Article 112 |
| issn | 0026-3672 1436-5073 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Analytical Chemistry Biosensors Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Copper Deoxyribonucleic acid Detectors DNA Electric properties Electrode materials Ethylenediaminetetraacetic acid Gold Graphene Graphite Hantavirus Metal-organic frameworks Microengineering Nanochemistry Nanoparticles Nanotechnology Nucleic acids Original Paper Phenylenediamine Self-assembly Signal generation Substrates |
| title | Electrochemical determination of hantavirus using gold nanoparticle-modified graphene as an electrode material and Cu-based metal-organic framework assisted signal generation |
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