Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth
The aim of this study was to investigate the effect of surface modification of a polycaprolactone scaffold on promoting osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Four different polycaprolactone scaffold were evaluated: untreated; coated with hyaluronic acid; coa...
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| Veröffentlicht in: | European journal of oral sciences 2021-04, Vol.129 (2), p.e12766-n/a |
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| creator | Jitpibull, Jirasak Tangjit, Nathaphon Dechkunakorn, Surachai Anuwongnukroh, Niwat Srikhirin, Toemsak Vongsetskul, Thammasit Sritanaudomchai, Hathaitip |
| description | The aim of this study was to investigate the effect of surface modification of a polycaprolactone scaffold on promoting osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Four different polycaprolactone scaffold were evaluated: untreated; coated with hyaluronic acid; coated with gelatin; and coated with hyaluronic acid and then with gelatin. The resulting scaffolds were characterized using scanning electron microscopy and attenuated total reflectance‐Fourier transform infrared spectroscopy (ATR‐FTIR). Human stem cells were cultured on the modified scaffolds placed in osteogenic differentiation medium. During culture, the osteogenic potential of the stem cells was examined by evaluating alkaline phosphatase activity and staining intensity, expression of osteoblastic‐specific genes, and matrix mineralization. Scanning electron microscopy and ATR‐FTIR confirmed productive biomacromolecular surface treatment of the polycaprolactone scaffold. All scaffolds permitted differentiation of stem cells into osteoblastic cells, but the gelatin‐coated polycaprolactone scaffold facilitated osteogenesis of a larger number of stem cells than the untreated and the hyaluronic acid‐coated scaffolds. We demonstrate that gelatin is an appropriate macromolecule for modifying the surface of an electrospun polycaprolactone fibre scaffold that is used subsequently in bone tissue engineering applications. |
| doi_str_mv | 10.1111/eos.12766 |
| format | Article |
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Four different polycaprolactone scaffold were evaluated: untreated; coated with hyaluronic acid; coated with gelatin; and coated with hyaluronic acid and then with gelatin. The resulting scaffolds were characterized using scanning electron microscopy and attenuated total reflectance‐Fourier transform infrared spectroscopy (ATR‐FTIR). Human stem cells were cultured on the modified scaffolds placed in osteogenic differentiation medium. During culture, the osteogenic potential of the stem cells was examined by evaluating alkaline phosphatase activity and staining intensity, expression of osteoblastic‐specific genes, and matrix mineralization. Scanning electron microscopy and ATR‐FTIR confirmed productive biomacromolecular surface treatment of the polycaprolactone scaffold. All scaffolds permitted differentiation of stem cells into osteoblastic cells, but the gelatin‐coated polycaprolactone scaffold facilitated osteogenesis of a larger number of stem cells than the untreated and the hyaluronic acid‐coated scaffolds. We demonstrate that gelatin is an appropriate macromolecule for modifying the surface of an electrospun polycaprolactone fibre scaffold that is used subsequently in bone tissue engineering applications.</description><identifier>ISSN: 0909-8836</identifier><identifier>EISSN: 1600-0722</identifier><identifier>DOI: 10.1111/eos.12766</identifier><identifier>PMID: 33667016</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Alkaline phosphatase ; Biomedical materials ; bone formation ; Cell culture ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Coating ; Coatings ; dental pulp stem cells ; Dentistry ; Differentiation (biology) ; Evaluation ; Fourier transforms ; Gelatin ; Gene expression ; Humans ; Hyaluronic acid ; Infrared spectroscopy ; Mineralization ; Osteoblastogenesis ; Osteoblasts ; Osteogenesis ; Polycaprolactone ; Polyesters ; Scaffolds ; Scanning electron microscopy ; Stem Cells ; Surface chemistry ; Surface treatment ; Teeth ; Tissue Engineering ; Tissue Scaffolds ; Tooth, Deciduous</subject><ispartof>European journal of oral sciences, 2021-04, Vol.129 (2), p.e12766-n/a</ispartof><rights>2021 European Journal of Oral Sciences</rights><rights>2021 European Journal of Oral Sciences.</rights><rights>Copyright © 2021 European Journal of Oral Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-b12bbea3828d4b537a629a6a2cc589907c43bae5c6d155a0a603e7095451ab403</citedby><cites>FETCH-LOGICAL-c3536-b12bbea3828d4b537a629a6a2cc589907c43bae5c6d155a0a603e7095451ab403</cites><orcidid>0000-0001-6171-3918</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Feos.12766$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Feos.12766$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33667016$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jitpibull, Jirasak</creatorcontrib><creatorcontrib>Tangjit, Nathaphon</creatorcontrib><creatorcontrib>Dechkunakorn, Surachai</creatorcontrib><creatorcontrib>Anuwongnukroh, Niwat</creatorcontrib><creatorcontrib>Srikhirin, Toemsak</creatorcontrib><creatorcontrib>Vongsetskul, Thammasit</creatorcontrib><creatorcontrib>Sritanaudomchai, Hathaitip</creatorcontrib><title>Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth</title><title>European journal of oral sciences</title><addtitle>Eur J Oral Sci</addtitle><description>The aim of this study was to investigate the effect of surface modification of a polycaprolactone scaffold on promoting osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Four different polycaprolactone scaffold were evaluated: untreated; coated with hyaluronic acid; coated with gelatin; and coated with hyaluronic acid and then with gelatin. The resulting scaffolds were characterized using scanning electron microscopy and attenuated total reflectance‐Fourier transform infrared spectroscopy (ATR‐FTIR). Human stem cells were cultured on the modified scaffolds placed in osteogenic differentiation medium. During culture, the osteogenic potential of the stem cells was examined by evaluating alkaline phosphatase activity and staining intensity, expression of osteoblastic‐specific genes, and matrix mineralization. Scanning electron microscopy and ATR‐FTIR confirmed productive biomacromolecular surface treatment of the polycaprolactone scaffold. All scaffolds permitted differentiation of stem cells into osteoblastic cells, but the gelatin‐coated polycaprolactone scaffold facilitated osteogenesis of a larger number of stem cells than the untreated and the hyaluronic acid‐coated scaffolds. We demonstrate that gelatin is an appropriate macromolecule for modifying the surface of an electrospun polycaprolactone fibre scaffold that is used subsequently in bone tissue engineering applications.</description><subject>Alkaline phosphatase</subject><subject>Biomedical materials</subject><subject>bone formation</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Coating</subject><subject>Coatings</subject><subject>dental pulp stem cells</subject><subject>Dentistry</subject><subject>Differentiation (biology)</subject><subject>Evaluation</subject><subject>Fourier transforms</subject><subject>Gelatin</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Hyaluronic acid</subject><subject>Infrared spectroscopy</subject><subject>Mineralization</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteogenesis</subject><subject>Polycaprolactone</subject><subject>Polyesters</subject><subject>Scaffolds</subject><subject>Scanning electron microscopy</subject><subject>Stem Cells</subject><subject>Surface chemistry</subject><subject>Surface treatment</subject><subject>Teeth</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds</subject><subject>Tooth, Deciduous</subject><issn>0909-8836</issn><issn>1600-0722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kbtuFDEUhi0EIkugyAsgS2lCMYkvY3tcRtFykSKlAGrL4znOOpoZL7ZHsF0eAYk35EniZRMKJE7j4nz-9B_9CJ1Qck7rXEDM55QpKZ-hFZWENEQx9hytiCa66Touj9CrnO8IoZxq9RIdcS6lIlSu0K-19-AKjh7nJXnrALsNTCGXtPt9_3OKQ_ABBryN487ZbYqjdSXOgLOz3sdxyDjOOOYC8Rbm4HDlPSSYS7Al7FdVXGDCDsYxY5_ihDfLZGcMP-r3ClX5AC4MS1wyLgBl8xq98HbM8ObxPUZf36-_XH1srm8-fLq6vG4cF1w2PWV9D5Z3rBvaXnBlJdNWWuac6LQmyrW8tyCcHKgQllhJOCiiRSuo7VvCj9HZwVvP-rZALqbevc9pZ6hhDGt11yolmK7o6T_oXVzSXNMZJohuW03Jnnp3oFyKOSfwZpvCZNPOUGL2TZnalPnTVGXfPhqXfoLhL_lUTQUuDsD3MMLu_yazvvl8UD4AYe6hCg</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Jitpibull, Jirasak</creator><creator>Tangjit, Nathaphon</creator><creator>Dechkunakorn, Surachai</creator><creator>Anuwongnukroh, Niwat</creator><creator>Srikhirin, Toemsak</creator><creator>Vongsetskul, Thammasit</creator><creator>Sritanaudomchai, Hathaitip</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6171-3918</orcidid></search><sort><creationdate>202104</creationdate><title>Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth</title><author>Jitpibull, Jirasak ; Tangjit, Nathaphon ; Dechkunakorn, Surachai ; Anuwongnukroh, Niwat ; Srikhirin, Toemsak ; Vongsetskul, Thammasit ; Sritanaudomchai, Hathaitip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-b12bbea3828d4b537a629a6a2cc589907c43bae5c6d155a0a603e7095451ab403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alkaline phosphatase</topic><topic>Biomedical materials</topic><topic>bone formation</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Coating</topic><topic>Coatings</topic><topic>dental pulp stem cells</topic><topic>Dentistry</topic><topic>Differentiation (biology)</topic><topic>Evaluation</topic><topic>Fourier transforms</topic><topic>Gelatin</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Hyaluronic acid</topic><topic>Infrared spectroscopy</topic><topic>Mineralization</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteogenesis</topic><topic>Polycaprolactone</topic><topic>Polyesters</topic><topic>Scaffolds</topic><topic>Scanning electron microscopy</topic><topic>Stem Cells</topic><topic>Surface chemistry</topic><topic>Surface treatment</topic><topic>Teeth</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds</topic><topic>Tooth, Deciduous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jitpibull, Jirasak</creatorcontrib><creatorcontrib>Tangjit, Nathaphon</creatorcontrib><creatorcontrib>Dechkunakorn, Surachai</creatorcontrib><creatorcontrib>Anuwongnukroh, Niwat</creatorcontrib><creatorcontrib>Srikhirin, Toemsak</creatorcontrib><creatorcontrib>Vongsetskul, Thammasit</creatorcontrib><creatorcontrib>Sritanaudomchai, Hathaitip</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of oral sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jitpibull, Jirasak</au><au>Tangjit, Nathaphon</au><au>Dechkunakorn, Surachai</au><au>Anuwongnukroh, Niwat</au><au>Srikhirin, Toemsak</au><au>Vongsetskul, Thammasit</au><au>Sritanaudomchai, Hathaitip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth</atitle><jtitle>European journal of oral sciences</jtitle><addtitle>Eur J Oral Sci</addtitle><date>2021-04</date><risdate>2021</risdate><volume>129</volume><issue>2</issue><spage>e12766</spage><epage>n/a</epage><pages>e12766-n/a</pages><issn>0909-8836</issn><eissn>1600-0722</eissn><abstract>The aim of this study was to investigate the effect of surface modification of a polycaprolactone scaffold on promoting osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Four different polycaprolactone scaffold were evaluated: untreated; coated with hyaluronic acid; coated with gelatin; and coated with hyaluronic acid and then with gelatin. The resulting scaffolds were characterized using scanning electron microscopy and attenuated total reflectance‐Fourier transform infrared spectroscopy (ATR‐FTIR). Human stem cells were cultured on the modified scaffolds placed in osteogenic differentiation medium. During culture, the osteogenic potential of the stem cells was examined by evaluating alkaline phosphatase activity and staining intensity, expression of osteoblastic‐specific genes, and matrix mineralization. Scanning electron microscopy and ATR‐FTIR confirmed productive biomacromolecular surface treatment of the polycaprolactone scaffold. All scaffolds permitted differentiation of stem cells into osteoblastic cells, but the gelatin‐coated polycaprolactone scaffold facilitated osteogenesis of a larger number of stem cells than the untreated and the hyaluronic acid‐coated scaffolds. We demonstrate that gelatin is an appropriate macromolecule for modifying the surface of an electrospun polycaprolactone fibre scaffold that is used subsequently in bone tissue engineering applications.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33667016</pmid><doi>10.1111/eos.12766</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6171-3918</orcidid></addata></record> |
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| identifier | ISSN: 0909-8836 |
| ispartof | European journal of oral sciences, 2021-04, Vol.129 (2), p.e12766-n/a |
| issn | 0909-8836 1600-0722 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Alkaline phosphatase Biomedical materials bone formation Cell culture Cell Differentiation Cell Proliferation Cells, Cultured Coating Coatings dental pulp stem cells Dentistry Differentiation (biology) Evaluation Fourier transforms Gelatin Gene expression Humans Hyaluronic acid Infrared spectroscopy Mineralization Osteoblastogenesis Osteoblasts Osteogenesis Polycaprolactone Polyesters Scaffolds Scanning electron microscopy Stem Cells Surface chemistry Surface treatment Teeth Tissue Engineering Tissue Scaffolds Tooth, Deciduous |
| title | Effect of surface chemistry‐modified polycaprolactone scaffolds on osteogenic differentiation of stem cells from human exfoliated deciduous teeth |
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