Lifetime-Engineered Ruby Nanoparticles (Tau-Rubies) for Multiplexed Imaging of μ‑Opioid Receptors
To address the growing demand for simultaneous imaging of multiple biomarkers in highly scattering media such as organotypic cell cultures, we introduce a new type of photoluminescent nanomaterial termed “tau-ruby” composed of ruby nanocrystals (Al2O3:Cr3+) with tunable emission lifetime. The lifeti...
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Veröffentlicht in: | ACS sensors 2021-03, Vol.6 (3), p.1375-1383 |
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creator | Yang, Xiaohong Maleki, Alireza Lipey, Nikolay A Zheng, Xianlin Santiago, Marina Connor, Mark Sreenivasan, Varun K. A Dawes, Judith M Lu, Yiqing Zvyagin, Andrei V |
description | To address the growing demand for simultaneous imaging of multiple biomarkers in highly scattering media such as organotypic cell cultures, we introduce a new type of photoluminescent nanomaterial termed “tau-ruby” composed of ruby nanocrystals (Al2O3:Cr3+) with tunable emission lifetime. The lifetime tuning range from 2.4 to 3.2 ms was achieved by varying the Cr3+ dopant concentration from 0.8% to 0.2%, affording facile implementation of background-free detection. We developed inexpensive scalable production of tau-ruby characterized by bright emission, narrow spectrum (693 ± 2 nm), and virtually unlimited photostability upon excitation with affordable excitation/detection sources, noncytotoxic and insensitive to microenvironmental fluctuations. By functionalizing the surface of tau-rubies with targeting antibodies, we obtained different biomarkers suitable for multiplexed lifetime imaging. As a proof of principle, three tau-ruby bioprobes, characterized by three mean lifetimes, were deployed to label three μ-opioid receptor species expressed on transfected cancer cells, each fused to a unique epitope, so that three types of cells were lifetime-encoded. Robust decoding of photoluminescent signals that report on each cell type was achieved by using a home-built lifetime imaging system and resulted in high-contrast multiplexed lifetime imaging of the cells. |
doi_str_mv | 10.1021/acssensors.1c00008 |
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We developed inexpensive scalable production of tau-ruby characterized by bright emission, narrow spectrum (693 ± 2 nm), and virtually unlimited photostability upon excitation with affordable excitation/detection sources, noncytotoxic and insensitive to microenvironmental fluctuations. By functionalizing the surface of tau-rubies with targeting antibodies, we obtained different biomarkers suitable for multiplexed lifetime imaging. As a proof of principle, three tau-ruby bioprobes, characterized by three mean lifetimes, were deployed to label three μ-opioid receptor species expressed on transfected cancer cells, each fused to a unique epitope, so that three types of cells were lifetime-encoded. 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A</au><au>Dawes, Judith M</au><au>Lu, Yiqing</au><au>Zvyagin, Andrei V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lifetime-Engineered Ruby Nanoparticles (Tau-Rubies) for Multiplexed Imaging of μ‑Opioid Receptors</atitle><jtitle>ACS sensors</jtitle><addtitle>ACS Sens</addtitle><date>2021-03-26</date><risdate>2021</risdate><volume>6</volume><issue>3</issue><spage>1375</spage><epage>1383</epage><pages>1375-1383</pages><issn>2379-3694</issn><eissn>2379-3694</eissn><abstract>To address the growing demand for simultaneous imaging of multiple biomarkers in highly scattering media such as organotypic cell cultures, we introduce a new type of photoluminescent nanomaterial termed “tau-ruby” composed of ruby nanocrystals (Al2O3:Cr3+) with tunable emission lifetime. The lifetime tuning range from 2.4 to 3.2 ms was achieved by varying the Cr3+ dopant concentration from 0.8% to 0.2%, affording facile implementation of background-free detection. 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title | Lifetime-Engineered Ruby Nanoparticles (Tau-Rubies) for Multiplexed Imaging of μ‑Opioid Receptors |
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