Evidence of bystander effect induced by radiofrequency radiation in a human neuroblastoma cell line
In previous studies we demonstrated that radiofrequency (RF) electromagnetic fields (EMF) is able to reduce DNA damage induced by a subsequent treatment with genotoxic agents, resembling the adaptive response, a phenomenon well known in radiobiology. In this study we report on the capability of the...
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| Veröffentlicht in: | Environmental research 2021-05, Vol.196, p.110935-110935, Article 110935 |
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| creator | Zeni, Olga Romeo, Stefania Sannino, Anna Palumbo, Rosanna Scarfì, Maria Rosaria |
| description | In previous studies we demonstrated that radiofrequency (RF) electromagnetic fields (EMF) is able to reduce DNA damage induced by a subsequent treatment with genotoxic agents, resembling the adaptive response, a phenomenon well known in radiobiology. In this study we report on the capability of the culture medium from SH-SY5Y neuroblastoma cells exposed to 1950 MHz to elicit, in recipient non-exposed cells, a reduction of menadione-induced DNA damage (P |
| doi_str_mv | 10.1016/j.envres.2021.110935 |
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•Radiofrequency (RF) radiation induced bystander effect in SH-SY5Y cells.•Beneficial instead of detrimental effect was detected.•A reduction of menadione-induced DNA damage was detected in cells treated with RF-conditioned medium (bystander effect).•A reduction of menadione-induced DNA damage was detected in cells directly exposed to RF (adaptive response).•An increased extracellular level of hsp70 was detected in RF-exposed cells with respect to sham-exposed ones.</description><identifier>ISSN: 0013-9351</identifier><identifier>EISSN: 1096-0953</identifier><identifier>DOI: 10.1016/j.envres.2021.110935</identifier><identifier>PMID: 33647301</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Bystander Effect ; Cell Line ; DNA Damage ; Electromagnetic Fields - adverse effects ; hsp70 levels ; Humans ; In vitro study ; Neuroblastoma ; Protective effect ; Radio Waves - adverse effects ; Radiofrequency</subject><ispartof>Environmental research, 2021-05, Vol.196, p.110935-110935, Article 110935</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-30350434fce47beb9e3abf0a5c42a99f140b194e5b390aa4d86c384034a66d563</citedby><cites>FETCH-LOGICAL-c362t-30350434fce47beb9e3abf0a5c42a99f140b194e5b390aa4d86c384034a66d563</cites><orcidid>0000-0002-9308-6928 ; 0000-0002-9849-3637</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0013935121002292$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33647301$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeni, Olga</creatorcontrib><creatorcontrib>Romeo, Stefania</creatorcontrib><creatorcontrib>Sannino, Anna</creatorcontrib><creatorcontrib>Palumbo, Rosanna</creatorcontrib><creatorcontrib>Scarfì, Maria Rosaria</creatorcontrib><title>Evidence of bystander effect induced by radiofrequency radiation in a human neuroblastoma cell line</title><title>Environmental research</title><addtitle>Environ Res</addtitle><description>In previous studies we demonstrated that radiofrequency (RF) electromagnetic fields (EMF) is able to reduce DNA damage induced by a subsequent treatment with genotoxic agents, resembling the adaptive response, a phenomenon well known in radiobiology. In this study we report on the capability of the culture medium from SH-SY5Y neuroblastoma cells exposed to 1950 MHz to elicit, in recipient non-exposed cells, a reduction of menadione-induced DNA damage (P < 0.05; comet assay), indicating the capability of non-ionizing radiation to elicit a bystander effect. A comparable reduction was also detected in cultures directly exposed to the same EMF conditions (P < 0.05), confirming the adaptive response. In the same exposure conditions, we also evidenced an increase of heat shock protein 70 (hsp70) in culture medium of cells exposed to RF with respect to sham exposed ones (P < 0.05; western blot analysis), while no differences were detected in the intracellular content of hsp70. On the whole, our results evidence a protective effect of RF against menadione-induced DNA damage in directly and non-directly exposed cells, and suggest hsp70 pathway to be investigated as one of the potential candidate underpinning the interaction between RF exposure and biological systems.
•Radiofrequency (RF) radiation induced bystander effect in SH-SY5Y cells.•Beneficial instead of detrimental effect was detected.•A reduction of menadione-induced DNA damage was detected in cells treated with RF-conditioned medium (bystander effect).•A reduction of menadione-induced DNA damage was detected in cells directly exposed to RF (adaptive response).•An increased extracellular level of hsp70 was detected in RF-exposed cells with respect to sham-exposed ones.</description><subject>Bystander Effect</subject><subject>Cell Line</subject><subject>DNA Damage</subject><subject>Electromagnetic Fields - adverse effects</subject><subject>hsp70 levels</subject><subject>Humans</subject><subject>In vitro study</subject><subject>Neuroblastoma</subject><subject>Protective effect</subject><subject>Radio Waves - adverse effects</subject><subject>Radiofrequency</subject><issn>0013-9351</issn><issn>1096-0953</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQgIMouj7-gUiOXrpOmrRuLoLI-gDBi55DmkwwS5to0i74781S9ehpmMw3mZmPkHMGSwasvdosMWwT5mUNNVsyBpI3e2RRYluBbPg-WQAwXpVndkSOc96UlDUcDskR56245sAWxKy33mIwSKOj3VcedbCYKDqHZqQ-2MmgLQWatPXRJfycCj2nevQxFIZq-j4NOtCAU4pdr_MYB00N9j3tfcBTcuB0n_HsJ56Qt_v1691j9fzy8HR3-1wZ3tZjxYE3ILhwBsV1h51ErjsHujGi1lI6JqBjUmDTcQlaC7tqDV8J4EK3rW1afkIu538_Uixr5lENPu-20AHjlFUtZCNArkRdUDGjJsWcEzr1kfyg05dioHZ61UbNetVOr5r1lraLnwlTN6D9a_r1WYCbGcBy59ZjUtn4nV7rUxGqbPT_T_gGTvaOFg</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Zeni, Olga</creator><creator>Romeo, Stefania</creator><creator>Sannino, Anna</creator><creator>Palumbo, Rosanna</creator><creator>Scarfì, Maria Rosaria</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9308-6928</orcidid><orcidid>https://orcid.org/0000-0002-9849-3637</orcidid></search><sort><creationdate>202105</creationdate><title>Evidence of bystander effect induced by radiofrequency radiation in a human neuroblastoma cell line</title><author>Zeni, Olga ; Romeo, Stefania ; Sannino, Anna ; Palumbo, Rosanna ; Scarfì, Maria Rosaria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-30350434fce47beb9e3abf0a5c42a99f140b194e5b390aa4d86c384034a66d563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Bystander Effect</topic><topic>Cell Line</topic><topic>DNA Damage</topic><topic>Electromagnetic Fields - adverse effects</topic><topic>hsp70 levels</topic><topic>Humans</topic><topic>In vitro study</topic><topic>Neuroblastoma</topic><topic>Protective effect</topic><topic>Radio Waves - adverse effects</topic><topic>Radiofrequency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeni, Olga</creatorcontrib><creatorcontrib>Romeo, Stefania</creatorcontrib><creatorcontrib>Sannino, Anna</creatorcontrib><creatorcontrib>Palumbo, Rosanna</creatorcontrib><creatorcontrib>Scarfì, Maria Rosaria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeni, Olga</au><au>Romeo, Stefania</au><au>Sannino, Anna</au><au>Palumbo, Rosanna</au><au>Scarfì, Maria Rosaria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence of bystander effect induced by radiofrequency radiation in a human neuroblastoma cell line</atitle><jtitle>Environmental research</jtitle><addtitle>Environ Res</addtitle><date>2021-05</date><risdate>2021</risdate><volume>196</volume><spage>110935</spage><epage>110935</epage><pages>110935-110935</pages><artnum>110935</artnum><issn>0013-9351</issn><eissn>1096-0953</eissn><abstract>In previous studies we demonstrated that radiofrequency (RF) electromagnetic fields (EMF) is able to reduce DNA damage induced by a subsequent treatment with genotoxic agents, resembling the adaptive response, a phenomenon well known in radiobiology. In this study we report on the capability of the culture medium from SH-SY5Y neuroblastoma cells exposed to 1950 MHz to elicit, in recipient non-exposed cells, a reduction of menadione-induced DNA damage (P < 0.05; comet assay), indicating the capability of non-ionizing radiation to elicit a bystander effect. A comparable reduction was also detected in cultures directly exposed to the same EMF conditions (P < 0.05), confirming the adaptive response. In the same exposure conditions, we also evidenced an increase of heat shock protein 70 (hsp70) in culture medium of cells exposed to RF with respect to sham exposed ones (P < 0.05; western blot analysis), while no differences were detected in the intracellular content of hsp70. On the whole, our results evidence a protective effect of RF against menadione-induced DNA damage in directly and non-directly exposed cells, and suggest hsp70 pathway to be investigated as one of the potential candidate underpinning the interaction between RF exposure and biological systems.
•Radiofrequency (RF) radiation induced bystander effect in SH-SY5Y cells.•Beneficial instead of detrimental effect was detected.•A reduction of menadione-induced DNA damage was detected in cells treated with RF-conditioned medium (bystander effect).•A reduction of menadione-induced DNA damage was detected in cells directly exposed to RF (adaptive response).•An increased extracellular level of hsp70 was detected in RF-exposed cells with respect to sham-exposed ones.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>33647301</pmid><doi>10.1016/j.envres.2021.110935</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9308-6928</orcidid><orcidid>https://orcid.org/0000-0002-9849-3637</orcidid></addata></record> |
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| subjects | Bystander Effect Cell Line DNA Damage Electromagnetic Fields - adverse effects hsp70 levels Humans In vitro study Neuroblastoma Protective effect Radio Waves - adverse effects Radiofrequency |
| title | Evidence of bystander effect induced by radiofrequency radiation in a human neuroblastoma cell line |
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