N-acetylcysteine attenuates PGE2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells
This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a...
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| Veröffentlicht in: | Dental Materials Journal 2021/05/25, Vol.40(3), pp.808-812 |
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| container_title | Dental Materials Journal |
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| creator | NAKAMURA, Koichi MINAMIKAWA, Hajime TAKAHASHI, Shizuka YOSHIMURA, Yoshitaka YAWAKA, Yasutaka |
| description | This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in αMEM (control) as well as on SB and SB in αMEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE2 production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification. |
| doi_str_mv | 10.4012/dmj.2020-275 |
| format | Article |
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Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in αMEM (control) as well as on SB and SB in αMEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE2 production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification.</description><identifier>ISSN: 0287-4547</identifier><identifier>EISSN: 1881-1361</identifier><identifier>DOI: 10.4012/dmj.2020-275</identifier><language>eng</language><publisher>Tokyo: The Japanese Society for Dental Materials and Devices</publisher><subject>4-META/MMA-based resin ; Acetylcysteine ; Biomedical materials ; Cell culture ; Culture media ; Cyclooxygenase-2 ; Dental materials ; Dentistry ; Detoxification ; Enzyme-linked immunosorbent assay ; Glutathione ; Inflammation ; Interleukin 6 ; Intracellular ; Methacryloyloxyethyl trimellitate anhydride ; N-acetylcysteine ; Osteoblasts ; Polymethyl methacrylate ; Prostaglandin E2 ; Reactive oxygen species ; Resins</subject><ispartof>Dental Materials Journal, 2021/05/25, Vol.40(3), pp.808-812</ispartof><rights>2021 The Japanese Society for Dental Materials and Devices</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c418t-66a18af6fc7149b8ca988168ee22543c60167f8b6cb39332f0899f87ffe254423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,1885,27931,27932</link.rule.ids></links><search><creatorcontrib>NAKAMURA, Koichi</creatorcontrib><creatorcontrib>MINAMIKAWA, Hajime</creatorcontrib><creatorcontrib>TAKAHASHI, Shizuka</creatorcontrib><creatorcontrib>YOSHIMURA, Yoshitaka</creatorcontrib><creatorcontrib>YAWAKA, Yasutaka</creatorcontrib><title>N-acetylcysteine attenuates PGE2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells</title><title>Dental Materials Journal</title><addtitle>Dent. Mater. J.</addtitle><description>This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in αMEM (control) as well as on SB and SB in αMEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE2 production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification.</description><subject>4-META/MMA-based resin</subject><subject>Acetylcysteine</subject><subject>Biomedical materials</subject><subject>Cell culture</subject><subject>Culture media</subject><subject>Cyclooxygenase-2</subject><subject>Dental materials</subject><subject>Dentistry</subject><subject>Detoxification</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Glutathione</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Intracellular</subject><subject>Methacryloyloxyethyl trimellitate anhydride</subject><subject>N-acetylcysteine</subject><subject>Osteoblasts</subject><subject>Polymethyl methacrylate</subject><subject>Prostaglandin E2</subject><subject>Reactive oxygen species</subject><subject>Resins</subject><issn>0287-4547</issn><issn>1881-1361</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkctOHDEQRS1EJAbCLh9gKRsWGPxqt70c0IQgMRAFsrbcnjL0qB9guxfz93FnAgskqyy5Tl1d10XoG6MXkjJ-uem3F5xySnhdHaAF05oRJhQ7RAvKdU1kJesjdJzSllJplNYLNN0T5yHvOr9LGdoBsMsZhsllSPjXzYpjN2zw74dH_BrHzeRzOw445bafuoJscLPDkqxXT8vL9XpJGpfKW4TUDricfoqz4liUx6ZzZcxjD12XvqIvwXUJTv_fJ-jPj9XT9U9y93Bze728I14ynYlSjmkXVPA1k6bR3pnyJaUBOK-k8IoyVQfdKN8IIwQPVBsTdB0ClL7k4gSd7XWL-bcJUrZ9m2YHboBxSpZLI7WmnMmCfv-EbscpDsWd5ZWojDBGm0Kd7ykfx5QiBPsa297FnWXUzhnYkoGdM7Alg4Jf7fFtyu4ZPmAXyyo6-AdLasVc3oc-mv7FRQuD-As0GZBV</recordid><startdate>20210525</startdate><enddate>20210525</enddate><creator>NAKAMURA, Koichi</creator><creator>MINAMIKAWA, Hajime</creator><creator>TAKAHASHI, Shizuka</creator><creator>YOSHIMURA, Yoshitaka</creator><creator>YAWAKA, Yasutaka</creator><general>The Japanese Society for Dental Materials and Devices</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20210525</creationdate><title>N-acetylcysteine attenuates PGE2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells</title><author>NAKAMURA, Koichi ; MINAMIKAWA, Hajime ; TAKAHASHI, Shizuka ; YOSHIMURA, Yoshitaka ; YAWAKA, Yasutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-66a18af6fc7149b8ca988168ee22543c60167f8b6cb39332f0899f87ffe254423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>4-META/MMA-based resin</topic><topic>Acetylcysteine</topic><topic>Biomedical materials</topic><topic>Cell culture</topic><topic>Culture media</topic><topic>Cyclooxygenase-2</topic><topic>Dental materials</topic><topic>Dentistry</topic><topic>Detoxification</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Glutathione</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Intracellular</topic><topic>Methacryloyloxyethyl trimellitate anhydride</topic><topic>N-acetylcysteine</topic><topic>Osteoblasts</topic><topic>Polymethyl methacrylate</topic><topic>Prostaglandin E2</topic><topic>Reactive oxygen species</topic><topic>Resins</topic><toplevel>online_resources</toplevel><creatorcontrib>NAKAMURA, Koichi</creatorcontrib><creatorcontrib>MINAMIKAWA, Hajime</creatorcontrib><creatorcontrib>TAKAHASHI, Shizuka</creatorcontrib><creatorcontrib>YOSHIMURA, Yoshitaka</creatorcontrib><creatorcontrib>YAWAKA, Yasutaka</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Dental Materials Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAKAMURA, Koichi</au><au>MINAMIKAWA, Hajime</au><au>TAKAHASHI, Shizuka</au><au>YOSHIMURA, Yoshitaka</au><au>YAWAKA, Yasutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-acetylcysteine attenuates PGE2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells</atitle><jtitle>Dental Materials Journal</jtitle><addtitle>Dent. Mater. J.</addtitle><date>2021-05-25</date><risdate>2021</risdate><volume>40</volume><issue>3</issue><spage>808</spage><epage>812</epage><pages>808-812</pages><issn>0287-4547</issn><eissn>1881-1361</eissn><abstract>This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in αMEM (control) as well as on SB and SB in αMEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE2 production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification.</abstract><cop>Tokyo</cop><pub>The Japanese Society for Dental Materials and Devices</pub><doi>10.4012/dmj.2020-275</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Dental Materials Journal, 2021/05/25, Vol.40(3), pp.808-812 |
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| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 4-META/MMA-based resin Acetylcysteine Biomedical materials Cell culture Culture media Cyclooxygenase-2 Dental materials Dentistry Detoxification Enzyme-linked immunosorbent assay Glutathione Inflammation Interleukin 6 Intracellular Methacryloyloxyethyl trimellitate anhydride N-acetylcysteine Osteoblasts Polymethyl methacrylate Prostaglandin E2 Reactive oxygen species Resins |
| title | N-acetylcysteine attenuates PGE2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells |
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