Small molecule-induced trinucleotide repeat contractions during in vitro DNA synthesis

Small molecule-induced trinucleotide repeat contractions during in vitro DNA synthesis

We demonstrated that a synthetic ligand NA, which selectively binds to a 5'-CAG-3'/5'-CAG-3' triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed t...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2021-04, Vol.57 (26), p.3235-3238
Hauptverfasser: Dohno, Chikara, Hagihara, Masaki, Binti Mohd Zaifuddin, Nursakinah, Nihei, Mizuki, Saito, Kaoru, Nakatani, Kazuhiko
Format: Artikel
Sprache:eng
Schlagworte:
DNA polymerase
Electrophoresis
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Zusammenfassung:We demonstrated that a synthetic ligand NA, which selectively binds to a 5'-CAG-3'/5'-CAG-3' triad, induced repeat contractions during DNA polymerase-mediated primer extension through the CAG repeat template. A thorough capillary electrophoresis and sequencing analysis revealed that the d(CAG) template gave shortened nascent strands mainly containing 3-6 CTG units in the presence of NA.
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc00349f