Photoinduced receptor confinement drives ligand-independent GPCR signaling
Cell-cell communication relies on the assembly of receptor-ligand complexes at the plasma membrane. The spatiotemporal receptor organization has a pivotal role in evoking cellular responses. We studied the clustering of heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2021-03, Vol.371 (6536) |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Sánchez, M Florencia Els-Heindl, Sylvia Beck-Sickinger, Annette G Wieneke, Ralph Tampé, Robert |
| description | Cell-cell communication relies on the assembly of receptor-ligand complexes at the plasma membrane. The spatiotemporal receptor organization has a pivotal role in evoking cellular responses. We studied the clustering of heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) and established a photoinstructive matrix with ultrasmall lock-and-key interaction pairs to control lateral membrane organization of hormone neuropeptide Y
receptors in living cells by light. Within seconds, receptor clustering was modulated in size, location, and density. After in situ confinement, changes in cellular morphology, motility, and calcium signaling revealed ligand-independent receptor activation. This approach may enhance the exploration of mechanisms in cell signaling and mechanotransduction. |
| doi_str_mv | 10.1126/science.abb7657 |
| format | Article |
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receptors in living cells by light. Within seconds, receptor clustering was modulated in size, location, and density. After in situ confinement, changes in cellular morphology, motility, and calcium signaling revealed ligand-independent receptor activation. This approach may enhance the exploration of mechanisms in cell signaling and mechanotransduction.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.abb7657</identifier><identifier>PMID: 33632896</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>Affinity ; Assembly ; Calcium ; Calcium - metabolism ; Calcium Signaling ; Cell adhesion & migration ; Cell Line ; Cell Membrane - metabolism ; Cell Movement ; Cells (biology) ; Chelating agents ; Clustering ; Confinement ; Coordination compounds ; Copolymers ; Cytosol - metabolism ; Diffusion ; Epilepsy ; G protein-coupled receptors ; Graft copolymers ; Histidine ; Human performance ; Humans ; Laser applications ; Lasers ; Ligands ; Light ; Lipids - analysis ; Luminous intensity ; Lysine ; Membranes ; Models, Biological ; Motility ; Neuropeptide Y - pharmacology ; Neuropeptides ; Nitrilotriacetic acid ; Nucleotides ; Photoactivation ; Physiology ; Proteins ; Receptor Aggregation ; Receptors, Neuropeptide Y - agonists ; Receptors, Neuropeptide Y - antagonists & inhibitors ; Receptors, Neuropeptide Y - metabolism ; Residential density ; Signal Transduction ; Spatial distribution ; Stability ; Topology</subject><ispartof>Science (American Association for the Advancement of Science), 2021-03, Vol.371 (6536)</ispartof><rights>Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.</rights><rights>Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-a102cbc58b051f3e65cb8c3e99b5381b9e24e3c6ea8c1ac85a3f89582ffe00bd3</citedby><cites>FETCH-LOGICAL-c391t-a102cbc58b051f3e65cb8c3e99b5381b9e24e3c6ea8c1ac85a3f89582ffe00bd3</cites><orcidid>0000-0003-1049-0368 ; 0000-0003-4560-8020 ; 0000-0002-1442-0335 ; 0000-0002-0767-4679 ; 0000-0002-0403-2160</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2871,2872,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33632896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sánchez, M Florencia</creatorcontrib><creatorcontrib>Els-Heindl, Sylvia</creatorcontrib><creatorcontrib>Beck-Sickinger, Annette G</creatorcontrib><creatorcontrib>Wieneke, Ralph</creatorcontrib><creatorcontrib>Tampé, Robert</creatorcontrib><title>Photoinduced receptor confinement drives ligand-independent GPCR signaling</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Cell-cell communication relies on the assembly of receptor-ligand complexes at the plasma membrane. The spatiotemporal receptor organization has a pivotal role in evoking cellular responses. We studied the clustering of heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) and established a photoinstructive matrix with ultrasmall lock-and-key interaction pairs to control lateral membrane organization of hormone neuropeptide Y
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| subjects | Affinity Assembly Calcium Calcium - metabolism Calcium Signaling Cell adhesion & migration Cell Line Cell Membrane - metabolism Cell Movement Cells (biology) Chelating agents Clustering Confinement Coordination compounds Copolymers Cytosol - metabolism Diffusion Epilepsy G protein-coupled receptors Graft copolymers Histidine Human performance Humans Laser applications Lasers Ligands Light Lipids - analysis Luminous intensity Lysine Membranes Models, Biological Motility Neuropeptide Y - pharmacology Neuropeptides Nitrilotriacetic acid Nucleotides Photoactivation Physiology Proteins Receptor Aggregation Receptors, Neuropeptide Y - agonists Receptors, Neuropeptide Y - antagonists & inhibitors Receptors, Neuropeptide Y - metabolism Residential density Signal Transduction Spatial distribution Stability Topology |
| title | Photoinduced receptor confinement drives ligand-independent GPCR signaling |
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