Density Matching Multi-wavelength Analytical Ultracentrifugation to Measure Drug Loading of Lipid Nanoparticle Formulations

Previous work suggested that lipid nanoparticle (LNP) formulations, encapsulating nucleic acids, display electron-dense morphology when examined by cryogenic-transmission electron microscopy (cryo-TEM). Critically, the employed cryo-TEM method cannot differentiate between loaded and empty LNP formul...

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Veröffentlicht in:	ACS nano 2021-03, Vol.15 (3), p.5068-5076
Hauptverfasser:	Henrickson, Amy, Kulkarni, Jayesh A, Zaifman, Josh, Gorbet, Gary E, Cullis, Pieter R, Demeler, Borries
Format:	Artikel
Sprache:	eng
Schlagworte:	Lipids Nanoparticles Nucleic Acids Pharmaceutical Preparations Ultracentrifugation
Online-Zugang:	Volltext
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