Effect of therapeutic UVC on corneal DNA: Safety assessment for potential keratitis treatment
Antimicrobial ultraviolet C (UVC) has proven efficacy in vitro against keratitis isolates and has potential to treat corneal infection if safety can be confirmed. Safety of 265 nm, 1.93 mW/cm2 intensity UVC (15–300 s exposures) was investigated in vitro via cyclobutane pyrimidine dimer (CPD) formati...
Gespeichert in:
| Veröffentlicht in: | The ocular surface 2021-04, Vol.20, p.130-138 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 138 |
|---|---|
| container_issue | |
| container_start_page | 130 |
| container_title | The ocular surface |
| container_volume | 20 |
| creator | Marasini, Sanjay Mugisho, Odunayo O. Swift, Simon Read, Hannah Rupenthal, Ilva D. Dean, Simon J. Craig, Jennifer P. |
| description | Antimicrobial ultraviolet C (UVC) has proven efficacy in vitro against keratitis isolates and has potential to treat corneal infection if safety can be confirmed.
Safety of 265 nm, 1.93 mW/cm2 intensity UVC (15–300 s exposures) was investigated in vitro via cyclobutane pyrimidine dimer (CPD) formation in DNA of human cultured corneal epithelial cells; ex vivo, by evaluating UVC transmissibility as a function of porcine corneal thickness; and in vivo, by evaluating CPD induction in the mouse cornea following UVC exposure.
A single exposure of 15 s UVC did not induce significant CPD formation (0.92 ± 1.45%) in vitro relative to untreated control (p = 0.93) whereas 300 s exposure caused extensive CPD formation (86.8 ± 13.73%; p |
| doi_str_mv | 10.1016/j.jtos.2021.02.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2492284536</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1542012421000057</els_id><sourcerecordid>2492284536</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-85676da5231353cc71b6035e19ec1b0ebd9938e2f747af4f59f832aad5ec888b3</originalsourceid><addsrcrecordid>eNp9kDtPwzAUhS0E4v0HGJBHlgQ_YidBLKiUh4RggLIhy3GuhUsbF9tF4t_jqoWR6Z7hO0e6H0InlJSUUHk-LafJx5IRRkvCSkLEFtqnLa8L0VK-nbOoWEEoq_bQQYxTQriUhO2iPc4lJXXF9tHb2FowCXuL0zsEvYBlcgZPXkfYD9j4MICe4evHqwv8rC2kb6xjhBjnMCRsfcALn3J0GfrI9eSSizgF0GlFHKEdq2cRjjf3EE1uxi-ju-Lh6fZ-dPVQGC5kKhoha9lrwTjlghtT004SLoC2YGhHoOvbljfAbF3V2lZWtLbhTOtegGmapuOH6Gy9uwj-cwkxqbmLBmYzPYBfRsWqlrGmElxmlK1RE3yMAaxaBDfX4VtRolZa1VSttKqVVkWYylpz6XSzv-zm0P9Vfj1m4HINQP7yy0FQ0TgYDPQuZL2q9-6__R_m0YlW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2492284536</pqid></control><display><type>article</type><title>Effect of therapeutic UVC on corneal DNA: Safety assessment for potential keratitis treatment</title><source>Alma/SFX Local Collection</source><creator>Marasini, Sanjay ; Mugisho, Odunayo O. ; Swift, Simon ; Read, Hannah ; Rupenthal, Ilva D. ; Dean, Simon J. ; Craig, Jennifer P.</creator><creatorcontrib>Marasini, Sanjay ; Mugisho, Odunayo O. ; Swift, Simon ; Read, Hannah ; Rupenthal, Ilva D. ; Dean, Simon J. ; Craig, Jennifer P.</creatorcontrib><description>Antimicrobial ultraviolet C (UVC) has proven efficacy in vitro against keratitis isolates and has potential to treat corneal infection if safety can be confirmed.
Safety of 265 nm, 1.93 mW/cm2 intensity UVC (15–300 s exposures) was investigated in vitro via cyclobutane pyrimidine dimer (CPD) formation in DNA of human cultured corneal epithelial cells; ex vivo, by evaluating UVC transmissibility as a function of porcine corneal thickness; and in vivo, by evaluating CPD induction in the mouse cornea following UVC exposure.
A single exposure of 15 s UVC did not induce significant CPD formation (0.92 ± 1.45%) in vitro relative to untreated control (p = 0.93) whereas 300 s exposure caused extensive CPD formation (86.8 ± 13.73%; p < 0.0001). Cumulative exposure to 15 s UVC daily for 3 days induced more CPD (14.6 ± 8.2%) than a single equivalent 45 s exposure (8.3 ± 4.0%) (p < 0.001) but levels returned to baseline within 72 h (p = 0.29), indicating highly efficient DNA repair. Ex vivo, UVC transmission decreased sharply with increasing corneal thickness, confirming UVC effects are limited to the superficial corneal layers. In vivo evaluations demonstrated no detectable CPD after three consecutive daily 15 s UVC exposures, whereas a single 300 s exposure induced extensive CPD formation in superficial corneal epithelium.
Up to three daily doses of 15 s UVC, in vivo, appear safe with respect to CPD formation. Ongoing research exploring UVC as a possible treatment for microbial keratitis is warranted.</description><identifier>ISSN: 1542-0124</identifier><identifier>EISSN: 1937-5913</identifier><identifier>DOI: 10.1016/j.jtos.2021.02.005</identifier><identifier>PMID: 33610742</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antimicrobial ; Keratitis ; Pseudomonas aeruginosa ; Treatment safety ; Ultraviolet C</subject><ispartof>The ocular surface, 2021-04, Vol.20, p.130-138</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-85676da5231353cc71b6035e19ec1b0ebd9938e2f747af4f59f832aad5ec888b3</citedby><cites>FETCH-LOGICAL-c356t-85676da5231353cc71b6035e19ec1b0ebd9938e2f747af4f59f832aad5ec888b3</cites><orcidid>0000-0001-7352-1112 ; 0000-0002-9540-5224 ; 0000-0001-8282-9637</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33610742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marasini, Sanjay</creatorcontrib><creatorcontrib>Mugisho, Odunayo O.</creatorcontrib><creatorcontrib>Swift, Simon</creatorcontrib><creatorcontrib>Read, Hannah</creatorcontrib><creatorcontrib>Rupenthal, Ilva D.</creatorcontrib><creatorcontrib>Dean, Simon J.</creatorcontrib><creatorcontrib>Craig, Jennifer P.</creatorcontrib><title>Effect of therapeutic UVC on corneal DNA: Safety assessment for potential keratitis treatment</title><title>The ocular surface</title><addtitle>Ocul Surf</addtitle><description>Antimicrobial ultraviolet C (UVC) has proven efficacy in vitro against keratitis isolates and has potential to treat corneal infection if safety can be confirmed.
Safety of 265 nm, 1.93 mW/cm2 intensity UVC (15–300 s exposures) was investigated in vitro via cyclobutane pyrimidine dimer (CPD) formation in DNA of human cultured corneal epithelial cells; ex vivo, by evaluating UVC transmissibility as a function of porcine corneal thickness; and in vivo, by evaluating CPD induction in the mouse cornea following UVC exposure.
A single exposure of 15 s UVC did not induce significant CPD formation (0.92 ± 1.45%) in vitro relative to untreated control (p = 0.93) whereas 300 s exposure caused extensive CPD formation (86.8 ± 13.73%; p < 0.0001). Cumulative exposure to 15 s UVC daily for 3 days induced more CPD (14.6 ± 8.2%) than a single equivalent 45 s exposure (8.3 ± 4.0%) (p < 0.001) but levels returned to baseline within 72 h (p = 0.29), indicating highly efficient DNA repair. Ex vivo, UVC transmission decreased sharply with increasing corneal thickness, confirming UVC effects are limited to the superficial corneal layers. In vivo evaluations demonstrated no detectable CPD after three consecutive daily 15 s UVC exposures, whereas a single 300 s exposure induced extensive CPD formation in superficial corneal epithelium.
Up to three daily doses of 15 s UVC, in vivo, appear safe with respect to CPD formation. Ongoing research exploring UVC as a possible treatment for microbial keratitis is warranted.</description><subject>Antimicrobial</subject><subject>Keratitis</subject><subject>Pseudomonas aeruginosa</subject><subject>Treatment safety</subject><subject>Ultraviolet C</subject><issn>1542-0124</issn><issn>1937-5913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kDtPwzAUhS0E4v0HGJBHlgQ_YidBLKiUh4RggLIhy3GuhUsbF9tF4t_jqoWR6Z7hO0e6H0InlJSUUHk-LafJx5IRRkvCSkLEFtqnLa8L0VK-nbOoWEEoq_bQQYxTQriUhO2iPc4lJXXF9tHb2FowCXuL0zsEvYBlcgZPXkfYD9j4MICe4evHqwv8rC2kb6xjhBjnMCRsfcALn3J0GfrI9eSSizgF0GlFHKEdq2cRjjf3EE1uxi-ju-Lh6fZ-dPVQGC5kKhoha9lrwTjlghtT004SLoC2YGhHoOvbljfAbF3V2lZWtLbhTOtegGmapuOH6Gy9uwj-cwkxqbmLBmYzPYBfRsWqlrGmElxmlK1RE3yMAaxaBDfX4VtRolZa1VSttKqVVkWYylpz6XSzv-zm0P9Vfj1m4HINQP7yy0FQ0TgYDPQuZL2q9-6__R_m0YlW</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Marasini, Sanjay</creator><creator>Mugisho, Odunayo O.</creator><creator>Swift, Simon</creator><creator>Read, Hannah</creator><creator>Rupenthal, Ilva D.</creator><creator>Dean, Simon J.</creator><creator>Craig, Jennifer P.</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7352-1112</orcidid><orcidid>https://orcid.org/0000-0002-9540-5224</orcidid><orcidid>https://orcid.org/0000-0001-8282-9637</orcidid></search><sort><creationdate>202104</creationdate><title>Effect of therapeutic UVC on corneal DNA: Safety assessment for potential keratitis treatment</title><author>Marasini, Sanjay ; Mugisho, Odunayo O. ; Swift, Simon ; Read, Hannah ; Rupenthal, Ilva D. ; Dean, Simon J. ; Craig, Jennifer P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-85676da5231353cc71b6035e19ec1b0ebd9938e2f747af4f59f832aad5ec888b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antimicrobial</topic><topic>Keratitis</topic><topic>Pseudomonas aeruginosa</topic><topic>Treatment safety</topic><topic>Ultraviolet C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marasini, Sanjay</creatorcontrib><creatorcontrib>Mugisho, Odunayo O.</creatorcontrib><creatorcontrib>Swift, Simon</creatorcontrib><creatorcontrib>Read, Hannah</creatorcontrib><creatorcontrib>Rupenthal, Ilva D.</creatorcontrib><creatorcontrib>Dean, Simon J.</creatorcontrib><creatorcontrib>Craig, Jennifer P.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The ocular surface</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marasini, Sanjay</au><au>Mugisho, Odunayo O.</au><au>Swift, Simon</au><au>Read, Hannah</au><au>Rupenthal, Ilva D.</au><au>Dean, Simon J.</au><au>Craig, Jennifer P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of therapeutic UVC on corneal DNA: Safety assessment for potential keratitis treatment</atitle><jtitle>The ocular surface</jtitle><addtitle>Ocul Surf</addtitle><date>2021-04</date><risdate>2021</risdate><volume>20</volume><spage>130</spage><epage>138</epage><pages>130-138</pages><issn>1542-0124</issn><eissn>1937-5913</eissn><abstract>Antimicrobial ultraviolet C (UVC) has proven efficacy in vitro against keratitis isolates and has potential to treat corneal infection if safety can be confirmed.
Safety of 265 nm, 1.93 mW/cm2 intensity UVC (15–300 s exposures) was investigated in vitro via cyclobutane pyrimidine dimer (CPD) formation in DNA of human cultured corneal epithelial cells; ex vivo, by evaluating UVC transmissibility as a function of porcine corneal thickness; and in vivo, by evaluating CPD induction in the mouse cornea following UVC exposure.
A single exposure of 15 s UVC did not induce significant CPD formation (0.92 ± 1.45%) in vitro relative to untreated control (p = 0.93) whereas 300 s exposure caused extensive CPD formation (86.8 ± 13.73%; p < 0.0001). Cumulative exposure to 15 s UVC daily for 3 days induced more CPD (14.6 ± 8.2%) than a single equivalent 45 s exposure (8.3 ± 4.0%) (p < 0.001) but levels returned to baseline within 72 h (p = 0.29), indicating highly efficient DNA repair. Ex vivo, UVC transmission decreased sharply with increasing corneal thickness, confirming UVC effects are limited to the superficial corneal layers. In vivo evaluations demonstrated no detectable CPD after three consecutive daily 15 s UVC exposures, whereas a single 300 s exposure induced extensive CPD formation in superficial corneal epithelium.
Up to three daily doses of 15 s UVC, in vivo, appear safe with respect to CPD formation. Ongoing research exploring UVC as a possible treatment for microbial keratitis is warranted.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33610742</pmid><doi>10.1016/j.jtos.2021.02.005</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7352-1112</orcidid><orcidid>https://orcid.org/0000-0002-9540-5224</orcidid><orcidid>https://orcid.org/0000-0001-8282-9637</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1542-0124 |
| ispartof | The ocular surface, 2021-04, Vol.20, p.130-138 |
| issn | 1542-0124 1937-5913 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2492284536 |
| source | Alma/SFX Local Collection |
| subjects | Antimicrobial Keratitis Pseudomonas aeruginosa Treatment safety Ultraviolet C |
| title | Effect of therapeutic UVC on corneal DNA: Safety assessment for potential keratitis treatment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T21%3A34%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20therapeutic%20UVC%20on%20corneal%20DNA:%20Safety%20assessment%20for%20potential%20keratitis%20treatment&rft.jtitle=The%20ocular%20surface&rft.au=Marasini,%20Sanjay&rft.date=2021-04&rft.volume=20&rft.spage=130&rft.epage=138&rft.pages=130-138&rft.issn=1542-0124&rft.eissn=1937-5913&rft_id=info:doi/10.1016/j.jtos.2021.02.005&rft_dat=%3Cproquest_cross%3E2492284536%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2492284536&rft_id=info:pmid/33610742&rft_els_id=S1542012421000057&rfr_iscdi=true |