Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22
Trichoderma orientalis ( T. orientalis ) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in Trichoderma reesei ( T. reesei ). However, the ace2 ge...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2021-07, Vol.193 (7), p.2098-2109 |
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| creator | Li, Yuyu Xue, Yong Liu, Jian Gan, Lihui Long, Minnan |
| description | Trichoderma orientalis
(
T. orientalis
) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in
Trichoderma reesei
(
T. reesei
). However, the
ace2
gene cannot be found in the genome of
T. orientalis
. Researches show that the mechanism of cellulase transcriptional regulation in
T. orientalis
keeps high similarity with
T. reesei
up till now. So, in this study, the
ace2
of
Trichoderma reesei
QM9414 was heterologous expressed in
T. orientalis
EU7-22. As a result, xylanase activity and β-glucosidase activity of
ace2
heterogeneous expression strains are improved and total cellulase activity is decreased. The result of qPCR is in accordance with enzyme activities. This study provides a reference for an in-depth study on transcriptional regulation mechanisms of
T. orientalis
. |
| doi_str_mv | 10.1007/s12010-021-03529-5 |
| format | Article |
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(
T. orientalis
) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in
Trichoderma reesei
(
T. reesei
). However, the
ace2
gene cannot be found in the genome of
T. orientalis
. Researches show that the mechanism of cellulase transcriptional regulation in
T. orientalis
keeps high similarity with
T. reesei
up till now. So, in this study, the
ace2
of
Trichoderma reesei
QM9414 was heterologous expressed in
T. orientalis
EU7-22. As a result, xylanase activity and β-glucosidase activity of
ace2
heterogeneous expression strains are improved and total cellulase activity is decreased. The result of qPCR is in accordance with enzyme activities. This study provides a reference for an in-depth study on transcriptional regulation mechanisms of
T. orientalis
.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-021-03529-5</identifier><identifier>PMID: 33608806</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>ACE2 ; ACE2 gene ; Angiotensin-converting enzyme 2 ; Biochemistry ; Biotechnology ; Cellobiase ; Cellulase ; Chemistry ; Chemistry and Materials Science ; endo-1,4-beta-glucanase ; Enzymatic activity ; Enzyme activity ; Enzymes ; Fungi ; Gene regulation ; genes ; Genomes ; Glucosidase ; Hemicellulases ; industry ; Original Article ; transcription (genetics) ; transcription factors ; Trichoderma ; Trichoderma reesei ; Xylanase ; xylanases ; β-Glucosidase</subject><ispartof>Applied biochemistry and biotechnology, 2021-07, Vol.193 (7), p.2098-2109</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-238cff3f4aeaa7c7e6bc9bfd80fcd953801aa27ea62a0e43d1332a3a798efe563</citedby><cites>FETCH-LOGICAL-c452t-238cff3f4aeaa7c7e6bc9bfd80fcd953801aa27ea62a0e43d1332a3a798efe563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-021-03529-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-021-03529-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33608806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yuyu</creatorcontrib><creatorcontrib>Xue, Yong</creatorcontrib><creatorcontrib>Liu, Jian</creatorcontrib><creatorcontrib>Gan, Lihui</creatorcontrib><creatorcontrib>Long, Minnan</creatorcontrib><title>Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Trichoderma orientalis
(
T. orientalis
) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in
Trichoderma reesei
(
T. reesei
). However, the
ace2
gene cannot be found in the genome of
T. orientalis
. Researches show that the mechanism of cellulase transcriptional regulation in
T. orientalis
keeps high similarity with
T. reesei
up till now. So, in this study, the
ace2
of
Trichoderma reesei
QM9414 was heterologous expressed in
T. orientalis
EU7-22. As a result, xylanase activity and β-glucosidase activity of
ace2
heterogeneous expression strains are improved and total cellulase activity is decreased. The result of qPCR is in accordance with enzyme activities. This study provides a reference for an in-depth study on transcriptional regulation mechanisms of
T. orientalis
.</description><subject>ACE2</subject><subject>ACE2 gene</subject><subject>Angiotensin-converting enzyme 2</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Cellobiase</subject><subject>Cellulase</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>endo-1,4-beta-glucanase</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Gene regulation</subject><subject>genes</subject><subject>Genomes</subject><subject>Glucosidase</subject><subject>Hemicellulases</subject><subject>industry</subject><subject>Original Article</subject><subject>transcription (genetics)</subject><subject>transcription factors</subject><subject>Trichoderma</subject><subject>Trichoderma reesei</subject><subject>Xylanase</subject><subject>xylanases</subject><subject>β-Glucosidase</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc9u1DAQxi0EarelL8ABWeLCJeA_cWwfq9WWVqrEpT1bs86YukrixU4keIa-NN5uKYIDPYxGmvl9n8f6CHnH2SfOmP5cuGCcNUzwhkklbKNekRVXytaR5a_JigktGyGMPSYnpdwzxoVR-ogcS9kxY1i3Ig-bENDPhaZA5zukNxmm4nPczTFN9AL8nDI99yhoyGms6-jvUo95BJoRC0ZasTUOwzJAQQpTTy9xjP55svmxy1jK3i1Of-lTjjjNMMRCN7e63vmWvAkwFDx76qfk9mJzs75srr9-uVqfXze-VWJuhDQ-BBlaQADtNXZbb7ehNyz43ippGAcQGqETwLCVPZdSgARtDQZUnTwlHw--u5y-L1hmN8ayvxgmTEtxous4a7ni_GW0tdy2iltb0Q__oPdpyVP9iBOq7bSpJSolDpTPqZSMwe1yHCH_dJy5farukKqrqbrHVJ2qovdP1st2xP5Z8jvGCsgDUOpq-ob5z9v_sf0F3wqtwQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Li, Yuyu</creator><creator>Xue, Yong</creator><creator>Liu, Jian</creator><creator>Gan, Lihui</creator><creator>Long, Minnan</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20210701</creationdate><title>Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22</title><author>Li, Yuyu ; Xue, Yong ; Liu, Jian ; Gan, Lihui ; Long, Minnan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-238cff3f4aeaa7c7e6bc9bfd80fcd953801aa27ea62a0e43d1332a3a798efe563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>ACE2</topic><topic>ACE2 gene</topic><topic>Angiotensin-converting enzyme 2</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Cellobiase</topic><topic>Cellulase</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>endo-1,4-beta-glucanase</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Gene regulation</topic><topic>genes</topic><topic>Genomes</topic><topic>Glucosidase</topic><topic>Hemicellulases</topic><topic>industry</topic><topic>Original Article</topic><topic>transcription (genetics)</topic><topic>transcription factors</topic><topic>Trichoderma</topic><topic>Trichoderma reesei</topic><topic>Xylanase</topic><topic>xylanases</topic><topic>β-Glucosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuyu</creatorcontrib><creatorcontrib>Xue, Yong</creatorcontrib><creatorcontrib>Liu, Jian</creatorcontrib><creatorcontrib>Gan, Lihui</creatorcontrib><creatorcontrib>Long, Minnan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuyu</au><au>Xue, Yong</au><au>Liu, Jian</au><au>Gan, Lihui</au><au>Long, Minnan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>193</volume><issue>7</issue><spage>2098</spage><epage>2109</epage><pages>2098-2109</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Trichoderma orientalis
(
T. orientalis
) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in
Trichoderma reesei
(
T. reesei
). However, the
ace2
gene cannot be found in the genome of
T. orientalis
. Researches show that the mechanism of cellulase transcriptional regulation in
T. orientalis
keeps high similarity with
T. reesei
up till now. So, in this study, the
ace2
of
Trichoderma reesei
QM9414 was heterologous expressed in
T. orientalis
EU7-22. As a result, xylanase activity and β-glucosidase activity of
ace2
heterogeneous expression strains are improved and total cellulase activity is decreased. The result of qPCR is in accordance with enzyme activities. This study provides a reference for an in-depth study on transcriptional regulation mechanisms of
T. orientalis
.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>33608806</pmid><doi>10.1007/s12010-021-03529-5</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 2021-07, Vol.193 (7), p.2098-2109 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2491945199 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | ACE2 ACE2 gene Angiotensin-converting enzyme 2 Biochemistry Biotechnology Cellobiase Cellulase Chemistry Chemistry and Materials Science endo-1,4-beta-glucanase Enzymatic activity Enzyme activity Enzymes Fungi Gene regulation genes Genomes Glucosidase Hemicellulases industry Original Article transcription (genetics) transcription factors Trichoderma Trichoderma reesei Xylanase xylanases β-Glucosidase |
| title | Effects of the Transcription Factor Ace2 from Trichoderma reesei on Cellulase and Hemicellulase Expression in Trichoderma orientalis EU7-22 |
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