Variants in TNF and NOS3 (eNOS) genes associated with sepsis in adult patients
Background Sepsis is a life‐threatening condition caused by a dysregulated host response to infections and is a leading cause of death in hospitalized patients. The present study aimed to elucidate the possible association between sepsis and the tumor necrosis factor (TNF) gene –308G/A (rs1800629) p...
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| Veröffentlicht in: | The journal of gene medicine 2021-04, Vol.23 (4), p.e3323-n/a |
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| creator | Özkan, Mustafa Günay, Nurullah Sener, Elif Funda Karcıoglu, Özgür Tahtasakal, Reyhan Dal, Fatma Günay, Nahide Ekici Demiryürek, Abdullah Tuncay |
| description | Background
Sepsis is a life‐threatening condition caused by a dysregulated host response to infections and is a leading cause of death in hospitalized patients. The present study aimed to elucidate the possible association between sepsis and the tumor necrosis factor (TNF) gene –308G/A (rs1800629) polymorphism, as well as endothelial nitric oxide synthase (eNOS, NOS3) gene –786T/C (rs2070744), 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms.
Methods
In total, 188 septic adult cases and 188 healthy controls were enrolled. Genomic DNAs from the controls and patients were analyzed by polymerase chain reaction and restriction fragment length polymorphism methods.
Results
There were significant associations between the G/G genotype and G allele of the TNF –308G/A (rs1800629) polymorphism in the sepsis group (p |
| doi_str_mv | 10.1002/jgm.3323 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2491942965</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2491942965</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3493-ef8b9bef127454caa826475635205072adb22404ccf516f10a7db58d9bb35edd3</originalsourceid><addsrcrecordid>eNp1kMtKAzEUhoMotlbBJ5CAm7qYmuvMZCnFVqW2C6u4C5lJpqbMpU5mKH1704sKgqv_LL7zcc4PwCVGA4wQuV0uigGlhB6BLuYEB4RwduxnJETARPzeAWfOLRHCURyLU9ChNESCEdwF0zdVW1U2DtoSzqcjqEoNp7MXCvvGxw1cmNI4qJyrUqsao-HaNh_QmZWzux2l27yBK9VY4y3n4CRTuTMXh-yB19H9fPgQTGbjx-HdJEgpEzQwWZyIxGSYRIyzVKmYhCziIeUEcRQRpRNCGGJpmnEcZhipSCc81iJJKDda0x7o772ruvpsjWtkYV1q8lyVpmqdJExg_6AIuUev_6DLqq1Lf50kHAlCUMTCX2FaV87VJpOr2haq3kiM5LZj6TuW2449enUQtklh9A_4XaoHgj2wtrnZ_CuST-PnnfALAviCXA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2509220746</pqid></control><display><type>article</type><title>Variants in TNF and NOS3 (eNOS) genes associated with sepsis in adult patients</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Özkan, Mustafa ; Günay, Nurullah ; Sener, Elif Funda ; Karcıoglu, Özgür ; Tahtasakal, Reyhan ; Dal, Fatma ; Günay, Nahide Ekici ; Demiryürek, Abdullah Tuncay</creator><creatorcontrib>Özkan, Mustafa ; Günay, Nurullah ; Sener, Elif Funda ; Karcıoglu, Özgür ; Tahtasakal, Reyhan ; Dal, Fatma ; Günay, Nahide Ekici ; Demiryürek, Abdullah Tuncay</creatorcontrib><description>Background
Sepsis is a life‐threatening condition caused by a dysregulated host response to infections and is a leading cause of death in hospitalized patients. The present study aimed to elucidate the possible association between sepsis and the tumor necrosis factor (TNF) gene –308G/A (rs1800629) polymorphism, as well as endothelial nitric oxide synthase (eNOS, NOS3) gene –786T/C (rs2070744), 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms.
Methods
In total, 188 septic adult cases and 188 healthy controls were enrolled. Genomic DNAs from the controls and patients were analyzed by polymerase chain reaction and restriction fragment length polymorphism methods.
Results
There were significant associations between the G/G genotype and G allele of the TNF –308G/A (rs1800629) polymorphism in the sepsis group (p < 0.001). The presence of the T/C genotype (p = 0.002) and C allele (p = 0.001) of the –786T/C (rs2070744) was markedly associated with an increased risk of sepsis. However, no significant associations were found with 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms. Higher 4bGC and lower 4bTT haplotype frequencies were associated with sepsis.
Conclusions
Our results strongly suggest that TNF gene (−308G/A, rs1800629) and NOS3 gene –786T/C (rs2070744) polymorphisms may modify individual susceptibility to sepsis in the Turkish population.
The presence of the G/G genotype and G allele of the TNF‐α –308G/A (rs1800629) polymorphism, as well as the T/C genotype and C allele of the NOS3 –786T/C (rs2070744) polymorphism, were markedly associated with an increased risk of sepsis. Our study provides the first evidence that there are marked associations between 4bGC and 4bTT haplotypes and sepsis. The results of the present study show that these polymorphisms may modify individual susceptibility to sepsis in the Turkish population.</description><identifier>ISSN: 1099-498X</identifier><identifier>EISSN: 1521-2254</identifier><identifier>DOI: 10.1002/jgm.3323</identifier><identifier>PMID: 33609421</identifier><language>eng</language><publisher>England: Wiley Periodicals Inc</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Alleles ; endothelial nitric oxide synthase ; Female ; Gene Frequency ; Gene polymorphism ; Gene therapy ; Genetic Association Studies ; Genetic Predisposition to Disease ; Genotype ; Haplotypes ; Haplotypes - genetics ; Humans ; Male ; Middle Aged ; Nitric oxide ; Nitric Oxide Synthase Type III - genetics ; Nitric-oxide synthase ; NOS3 protein ; Polymerase chain reaction ; Polymorphism ; Polymorphism, Single Nucleotide - genetics ; Restriction fragment length polymorphism ; Risk Factors ; Sepsis ; Sepsis - blood ; Sepsis - genetics ; Sepsis - pathology ; susceptibility ; Tumor necrosis factor ; Tumor Necrosis Factor-alpha - genetics ; Tumor necrosis factor-TNF ; Young Adult</subject><ispartof>The journal of gene medicine, 2021-04, Vol.23 (4), p.e3323-n/a</ispartof><rights>2021 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3493-ef8b9bef127454caa826475635205072adb22404ccf516f10a7db58d9bb35edd3</citedby><cites>FETCH-LOGICAL-c3493-ef8b9bef127454caa826475635205072adb22404ccf516f10a7db58d9bb35edd3</cites><orcidid>0000-0003-1604-9565 ; 0000-0002-5644-5442</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjgm.3323$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjgm.3323$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33609421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Özkan, Mustafa</creatorcontrib><creatorcontrib>Günay, Nurullah</creatorcontrib><creatorcontrib>Sener, Elif Funda</creatorcontrib><creatorcontrib>Karcıoglu, Özgür</creatorcontrib><creatorcontrib>Tahtasakal, Reyhan</creatorcontrib><creatorcontrib>Dal, Fatma</creatorcontrib><creatorcontrib>Günay, Nahide Ekici</creatorcontrib><creatorcontrib>Demiryürek, Abdullah Tuncay</creatorcontrib><title>Variants in TNF and NOS3 (eNOS) genes associated with sepsis in adult patients</title><title>The journal of gene medicine</title><addtitle>J Gene Med</addtitle><description>Background
Sepsis is a life‐threatening condition caused by a dysregulated host response to infections and is a leading cause of death in hospitalized patients. The present study aimed to elucidate the possible association between sepsis and the tumor necrosis factor (TNF) gene –308G/A (rs1800629) polymorphism, as well as endothelial nitric oxide synthase (eNOS, NOS3) gene –786T/C (rs2070744), 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms.
Methods
In total, 188 septic adult cases and 188 healthy controls were enrolled. Genomic DNAs from the controls and patients were analyzed by polymerase chain reaction and restriction fragment length polymorphism methods.
Results
There were significant associations between the G/G genotype and G allele of the TNF –308G/A (rs1800629) polymorphism in the sepsis group (p < 0.001). The presence of the T/C genotype (p = 0.002) and C allele (p = 0.001) of the –786T/C (rs2070744) was markedly associated with an increased risk of sepsis. However, no significant associations were found with 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms. Higher 4bGC and lower 4bTT haplotype frequencies were associated with sepsis.
Conclusions
Our results strongly suggest that TNF gene (−308G/A, rs1800629) and NOS3 gene –786T/C (rs2070744) polymorphisms may modify individual susceptibility to sepsis in the Turkish population.
The presence of the G/G genotype and G allele of the TNF‐α –308G/A (rs1800629) polymorphism, as well as the T/C genotype and C allele of the NOS3 –786T/C (rs2070744) polymorphism, were markedly associated with an increased risk of sepsis. Our study provides the first evidence that there are marked associations between 4bGC and 4bTT haplotypes and sepsis. The results of the present study show that these polymorphisms may modify individual susceptibility to sepsis in the Turkish population.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alleles</subject><subject>endothelial nitric oxide synthase</subject><subject>Female</subject><subject>Gene Frequency</subject><subject>Gene polymorphism</subject><subject>Gene therapy</subject><subject>Genetic Association Studies</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotype</subject><subject>Haplotypes</subject><subject>Haplotypes - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase Type III - genetics</subject><subject>Nitric-oxide synthase</subject><subject>NOS3 protein</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Restriction fragment length polymorphism</subject><subject>Risk Factors</subject><subject>Sepsis</subject><subject>Sepsis - blood</subject><subject>Sepsis - genetics</subject><subject>Sepsis - pathology</subject><subject>susceptibility</subject><subject>Tumor necrosis factor</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor necrosis factor-TNF</subject><subject>Young Adult</subject><issn>1099-498X</issn><issn>1521-2254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKAzEUhoMotlbBJ5CAm7qYmuvMZCnFVqW2C6u4C5lJpqbMpU5mKH1704sKgqv_LL7zcc4PwCVGA4wQuV0uigGlhB6BLuYEB4RwduxnJETARPzeAWfOLRHCURyLU9ChNESCEdwF0zdVW1U2DtoSzqcjqEoNp7MXCvvGxw1cmNI4qJyrUqsao-HaNh_QmZWzux2l27yBK9VY4y3n4CRTuTMXh-yB19H9fPgQTGbjx-HdJEgpEzQwWZyIxGSYRIyzVKmYhCziIeUEcRQRpRNCGGJpmnEcZhipSCc81iJJKDda0x7o772ruvpsjWtkYV1q8lyVpmqdJExg_6AIuUev_6DLqq1Lf50kHAlCUMTCX2FaV87VJpOr2haq3kiM5LZj6TuW2449enUQtklh9A_4XaoHgj2wtrnZ_CuST-PnnfALAviCXA</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Özkan, Mustafa</creator><creator>Günay, Nurullah</creator><creator>Sener, Elif Funda</creator><creator>Karcıoglu, Özgür</creator><creator>Tahtasakal, Reyhan</creator><creator>Dal, Fatma</creator><creator>Günay, Nahide Ekici</creator><creator>Demiryürek, Abdullah Tuncay</creator><general>Wiley Periodicals Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1604-9565</orcidid><orcidid>https://orcid.org/0000-0002-5644-5442</orcidid></search><sort><creationdate>202104</creationdate><title>Variants in TNF and NOS3 (eNOS) genes associated with sepsis in adult patients</title><author>Özkan, Mustafa ; Günay, Nurullah ; Sener, Elif Funda ; Karcıoglu, Özgür ; Tahtasakal, Reyhan ; Dal, Fatma ; Günay, Nahide Ekici ; Demiryürek, Abdullah Tuncay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3493-ef8b9bef127454caa826475635205072adb22404ccf516f10a7db58d9bb35edd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alleles</topic><topic>endothelial nitric oxide synthase</topic><topic>Female</topic><topic>Gene Frequency</topic><topic>Gene polymorphism</topic><topic>Gene therapy</topic><topic>Genetic Association Studies</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotype</topic><topic>Haplotypes</topic><topic>Haplotypes - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nitric oxide</topic><topic>Nitric Oxide Synthase Type III - genetics</topic><topic>Nitric-oxide synthase</topic><topic>NOS3 protein</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Restriction fragment length polymorphism</topic><topic>Risk Factors</topic><topic>Sepsis</topic><topic>Sepsis - blood</topic><topic>Sepsis - genetics</topic><topic>Sepsis - pathology</topic><topic>susceptibility</topic><topic>Tumor necrosis factor</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor necrosis factor-TNF</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Özkan, Mustafa</creatorcontrib><creatorcontrib>Günay, Nurullah</creatorcontrib><creatorcontrib>Sener, Elif Funda</creatorcontrib><creatorcontrib>Karcıoglu, Özgür</creatorcontrib><creatorcontrib>Tahtasakal, Reyhan</creatorcontrib><creatorcontrib>Dal, Fatma</creatorcontrib><creatorcontrib>Günay, Nahide Ekici</creatorcontrib><creatorcontrib>Demiryürek, Abdullah Tuncay</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of gene medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Özkan, Mustafa</au><au>Günay, Nurullah</au><au>Sener, Elif Funda</au><au>Karcıoglu, Özgür</au><au>Tahtasakal, Reyhan</au><au>Dal, Fatma</au><au>Günay, Nahide Ekici</au><au>Demiryürek, Abdullah Tuncay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variants in TNF and NOS3 (eNOS) genes associated with sepsis in adult patients</atitle><jtitle>The journal of gene medicine</jtitle><addtitle>J Gene Med</addtitle><date>2021-04</date><risdate>2021</risdate><volume>23</volume><issue>4</issue><spage>e3323</spage><epage>n/a</epage><pages>e3323-n/a</pages><issn>1099-498X</issn><eissn>1521-2254</eissn><abstract>Background
Sepsis is a life‐threatening condition caused by a dysregulated host response to infections and is a leading cause of death in hospitalized patients. The present study aimed to elucidate the possible association between sepsis and the tumor necrosis factor (TNF) gene –308G/A (rs1800629) polymorphism, as well as endothelial nitric oxide synthase (eNOS, NOS3) gene –786T/C (rs2070744), 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms.
Methods
In total, 188 septic adult cases and 188 healthy controls were enrolled. Genomic DNAs from the controls and patients were analyzed by polymerase chain reaction and restriction fragment length polymorphism methods.
Results
There were significant associations between the G/G genotype and G allele of the TNF –308G/A (rs1800629) polymorphism in the sepsis group (p < 0.001). The presence of the T/C genotype (p = 0.002) and C allele (p = 0.001) of the –786T/C (rs2070744) was markedly associated with an increased risk of sepsis. However, no significant associations were found with 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms. Higher 4bGC and lower 4bTT haplotype frequencies were associated with sepsis.
Conclusions
Our results strongly suggest that TNF gene (−308G/A, rs1800629) and NOS3 gene –786T/C (rs2070744) polymorphisms may modify individual susceptibility to sepsis in the Turkish population.
The presence of the G/G genotype and G allele of the TNF‐α –308G/A (rs1800629) polymorphism, as well as the T/C genotype and C allele of the NOS3 –786T/C (rs2070744) polymorphism, were markedly associated with an increased risk of sepsis. Our study provides the first evidence that there are marked associations between 4bGC and 4bTT haplotypes and sepsis. The results of the present study show that these polymorphisms may modify individual susceptibility to sepsis in the Turkish population.</abstract><cop>England</cop><pub>Wiley Periodicals Inc</pub><pmid>33609421</pmid><doi>10.1002/jgm.3323</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1604-9565</orcidid><orcidid>https://orcid.org/0000-0002-5644-5442</orcidid></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Alleles endothelial nitric oxide synthase Female Gene Frequency Gene polymorphism Gene therapy Genetic Association Studies Genetic Predisposition to Disease Genotype Haplotypes Haplotypes - genetics Humans Male Middle Aged Nitric oxide Nitric Oxide Synthase Type III - genetics Nitric-oxide synthase NOS3 protein Polymerase chain reaction Polymorphism Polymorphism, Single Nucleotide - genetics Restriction fragment length polymorphism Risk Factors Sepsis Sepsis - blood Sepsis - genetics Sepsis - pathology susceptibility Tumor necrosis factor Tumor Necrosis Factor-alpha - genetics Tumor necrosis factor-TNF Young Adult |
| title | Variants in TNF and NOS3 (eNOS) genes associated with sepsis in adult patients |
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