Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification
We construct a multiplex surface-enhanced Raman scattering (SERS) platform based on a plasmonic paper substrate and a double-labeled probe for the detection of multiple fluorescent dyes at high sensitivity in a single-wavelength light source system. Plasmonic paper, made of silver nanodots on three-...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2021-03, Vol.93 (8), p.3677-3685 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3685 |
|---|---|
| container_issue | 8 |
| container_start_page | 3677 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 93 |
| creator | Kim, Eun Ju Kim, Hanbi Park, Eunkyoung Kim, Taekyung Chung, Doo Ryeon Choi, Young-Man Kang, Minhee |
| description | We construct a multiplex surface-enhanced Raman scattering (SERS) platform based on a plasmonic paper substrate and a double-labeled probe for the detection of multiple fluorescent dyes at high sensitivity in a single-wavelength light source system. Plasmonic paper, made of silver nanodots on three-dimensional cellulose fibers, enables highly sensitive SERS biosensing based on localized surface plasmon resonance (LSPR). The proposed method enables the identification and quantification of a range of fluorescent dyes ranging from picomolar to millimolar concentrations. The use of 5′ fluorescent dyes and 3′ biotin-modified probes as SERS-coded probes renders possible the separation of fluorescent dyes with streptavidin-coated magnetic beads (SMBs) and the sensitive detection of multiple dyes after the reverse transcription polymerase chain reaction (RT-PCR). This experimental study reveals the multiplex detection capability of PCR-based SERS under existing PCR conditions without modifying primer and probe sequences. The combination of magnetic bead-based separation and paper SERS platform is efficient, economical, and can be used for the simultaneous detection of two or more pathogens. |
| doi_str_mv | 10.1021/acs.analchem.0c05285 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2491939891</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2491939891</sourcerecordid><originalsourceid>FETCH-LOGICAL-a422t-ffcde0bb57f22585b5ccfb3773e608c9bf460badacf347a32d949ad8c3f0f933</originalsourceid><addsrcrecordid>eNp9kctOwzAQRS0EglL4A4QisWGTMrbzXEJ5SkVUbVlHE2dMg_IodiLB3-PS0gULVpZnzr2WfBg74zDiIPgVKjvCBiu1pHoECkKRhHtswEMBfpQkYp8NAED6IgY4YsfWvgNwDjw6ZEdSRhCFwAesm-KKjH-Dlgrvua-6clXRpzfvjUZF_l2zxEa51QxrbLy5wq4jUzZv3i11pLqybbxXu75P2-qrJuN6vPESy8abEW72U9PmbtgWpS5d3o1O2IHGytLp9hyyxf3dYvzoT14ensbXEx8DITpfa1UQ5HkYayHCJMxDpXQu41hSBIlKcx1EkGOBSssgRimKNEixSJTUoFMph-xyU7sy7UdPtsvq0iqqKmyo7W0mgpSnMk1S7tCLP-h72xv3uz9UEgguReCoYEMp01prSGcrU9ZovjIO2VpK5qRkv1KyrRQXO9-W93lNxS70a8EBsAHW8d3D_3Z-AzI5nR8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2498421324</pqid></control><display><type>article</type><title>Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification</title><source>American Chemical Society Journals</source><creator>Kim, Eun Ju ; Kim, Hanbi ; Park, Eunkyoung ; Kim, Taekyung ; Chung, Doo Ryeon ; Choi, Young-Man ; Kang, Minhee</creator><creatorcontrib>Kim, Eun Ju ; Kim, Hanbi ; Park, Eunkyoung ; Kim, Taekyung ; Chung, Doo Ryeon ; Choi, Young-Man ; Kang, Minhee</creatorcontrib><description>We construct a multiplex surface-enhanced Raman scattering (SERS) platform based on a plasmonic paper substrate and a double-labeled probe for the detection of multiple fluorescent dyes at high sensitivity in a single-wavelength light source system. Plasmonic paper, made of silver nanodots on three-dimensional cellulose fibers, enables highly sensitive SERS biosensing based on localized surface plasmon resonance (LSPR). The proposed method enables the identification and quantification of a range of fluorescent dyes ranging from picomolar to millimolar concentrations. The use of 5′ fluorescent dyes and 3′ biotin-modified probes as SERS-coded probes renders possible the separation of fluorescent dyes with streptavidin-coated magnetic beads (SMBs) and the sensitive detection of multiple dyes after the reverse transcription polymerase chain reaction (RT-PCR). This experimental study reveals the multiplex detection capability of PCR-based SERS under existing PCR conditions without modifying primer and probe sequences. The combination of magnetic bead-based separation and paper SERS platform is efficient, economical, and can be used for the simultaneous detection of two or more pathogens.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.0c05285</identifier><identifier>PMID: 33606501</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Beads ; Biosensors ; Biotin ; Cellulose ; Cellulose fibers ; Chemistry ; Codification ; Dyes ; Fluorescent dyes ; Fluorescent indicators ; Light sources ; Multiplexing ; Plasmonics ; Polymerase chain reaction ; Primers (coatings) ; Probes ; Raman spectra ; Reverse transcription ; Separation ; Streptavidin ; Substrates ; Surface plasmon resonance</subject><ispartof>Analytical chemistry (Washington), 2021-03, Vol.93 (8), p.3677-3685</ispartof><rights>2021 The Authors. Published by American Chemical Society</rights><rights>Copyright American Chemical Society Mar 2, 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a422t-ffcde0bb57f22585b5ccfb3773e608c9bf460badacf347a32d949ad8c3f0f933</citedby><cites>FETCH-LOGICAL-a422t-ffcde0bb57f22585b5ccfb3773e608c9bf460badacf347a32d949ad8c3f0f933</cites><orcidid>0000-0003-0330-7828</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c05285$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c05285$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33606501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Eun Ju</creatorcontrib><creatorcontrib>Kim, Hanbi</creatorcontrib><creatorcontrib>Park, Eunkyoung</creatorcontrib><creatorcontrib>Kim, Taekyung</creatorcontrib><creatorcontrib>Chung, Doo Ryeon</creatorcontrib><creatorcontrib>Choi, Young-Man</creatorcontrib><creatorcontrib>Kang, Minhee</creatorcontrib><title>Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>We construct a multiplex surface-enhanced Raman scattering (SERS) platform based on a plasmonic paper substrate and a double-labeled probe for the detection of multiple fluorescent dyes at high sensitivity in a single-wavelength light source system. Plasmonic paper, made of silver nanodots on three-dimensional cellulose fibers, enables highly sensitive SERS biosensing based on localized surface plasmon resonance (LSPR). The proposed method enables the identification and quantification of a range of fluorescent dyes ranging from picomolar to millimolar concentrations. The use of 5′ fluorescent dyes and 3′ biotin-modified probes as SERS-coded probes renders possible the separation of fluorescent dyes with streptavidin-coated magnetic beads (SMBs) and the sensitive detection of multiple dyes after the reverse transcription polymerase chain reaction (RT-PCR). This experimental study reveals the multiplex detection capability of PCR-based SERS under existing PCR conditions without modifying primer and probe sequences. The combination of magnetic bead-based separation and paper SERS platform is efficient, economical, and can be used for the simultaneous detection of two or more pathogens.</description><subject>Analytical chemistry</subject><subject>Beads</subject><subject>Biosensors</subject><subject>Biotin</subject><subject>Cellulose</subject><subject>Cellulose fibers</subject><subject>Chemistry</subject><subject>Codification</subject><subject>Dyes</subject><subject>Fluorescent dyes</subject><subject>Fluorescent indicators</subject><subject>Light sources</subject><subject>Multiplexing</subject><subject>Plasmonics</subject><subject>Polymerase chain reaction</subject><subject>Primers (coatings)</subject><subject>Probes</subject><subject>Raman spectra</subject><subject>Reverse transcription</subject><subject>Separation</subject><subject>Streptavidin</subject><subject>Substrates</subject><subject>Surface plasmon resonance</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kctOwzAQRS0EglL4A4QisWGTMrbzXEJ5SkVUbVlHE2dMg_IodiLB3-PS0gULVpZnzr2WfBg74zDiIPgVKjvCBiu1pHoECkKRhHtswEMBfpQkYp8NAED6IgY4YsfWvgNwDjw6ZEdSRhCFwAesm-KKjH-Dlgrvua-6clXRpzfvjUZF_l2zxEa51QxrbLy5wq4jUzZv3i11pLqybbxXu75P2-qrJuN6vPESy8abEW72U9PmbtgWpS5d3o1O2IHGytLp9hyyxf3dYvzoT14ensbXEx8DITpfa1UQ5HkYayHCJMxDpXQu41hSBIlKcx1EkGOBSssgRimKNEixSJTUoFMph-xyU7sy7UdPtsvq0iqqKmyo7W0mgpSnMk1S7tCLP-h72xv3uz9UEgguReCoYEMp01prSGcrU9ZovjIO2VpK5qRkv1KyrRQXO9-W93lNxS70a8EBsAHW8d3D_3Z-AzI5nR8</recordid><startdate>20210302</startdate><enddate>20210302</enddate><creator>Kim, Eun Ju</creator><creator>Kim, Hanbi</creator><creator>Park, Eunkyoung</creator><creator>Kim, Taekyung</creator><creator>Chung, Doo Ryeon</creator><creator>Choi, Young-Man</creator><creator>Kang, Minhee</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0330-7828</orcidid></search><sort><creationdate>20210302</creationdate><title>Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification</title><author>Kim, Eun Ju ; Kim, Hanbi ; Park, Eunkyoung ; Kim, Taekyung ; Chung, Doo Ryeon ; Choi, Young-Man ; Kang, Minhee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a422t-ffcde0bb57f22585b5ccfb3773e608c9bf460badacf347a32d949ad8c3f0f933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analytical chemistry</topic><topic>Beads</topic><topic>Biosensors</topic><topic>Biotin</topic><topic>Cellulose</topic><topic>Cellulose fibers</topic><topic>Chemistry</topic><topic>Codification</topic><topic>Dyes</topic><topic>Fluorescent dyes</topic><topic>Fluorescent indicators</topic><topic>Light sources</topic><topic>Multiplexing</topic><topic>Plasmonics</topic><topic>Polymerase chain reaction</topic><topic>Primers (coatings)</topic><topic>Probes</topic><topic>Raman spectra</topic><topic>Reverse transcription</topic><topic>Separation</topic><topic>Streptavidin</topic><topic>Substrates</topic><topic>Surface plasmon resonance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Eun Ju</creatorcontrib><creatorcontrib>Kim, Hanbi</creatorcontrib><creatorcontrib>Park, Eunkyoung</creatorcontrib><creatorcontrib>Kim, Taekyung</creatorcontrib><creatorcontrib>Chung, Doo Ryeon</creatorcontrib><creatorcontrib>Choi, Young-Man</creatorcontrib><creatorcontrib>Kang, Minhee</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Eun Ju</au><au>Kim, Hanbi</au><au>Park, Eunkyoung</au><au>Kim, Taekyung</au><au>Chung, Doo Ryeon</au><au>Choi, Young-Man</au><au>Kang, Minhee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2021-03-02</date><risdate>2021</risdate><volume>93</volume><issue>8</issue><spage>3677</spage><epage>3685</epage><pages>3677-3685</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>We construct a multiplex surface-enhanced Raman scattering (SERS) platform based on a plasmonic paper substrate and a double-labeled probe for the detection of multiple fluorescent dyes at high sensitivity in a single-wavelength light source system. Plasmonic paper, made of silver nanodots on three-dimensional cellulose fibers, enables highly sensitive SERS biosensing based on localized surface plasmon resonance (LSPR). The proposed method enables the identification and quantification of a range of fluorescent dyes ranging from picomolar to millimolar concentrations. The use of 5′ fluorescent dyes and 3′ biotin-modified probes as SERS-coded probes renders possible the separation of fluorescent dyes with streptavidin-coated magnetic beads (SMBs) and the sensitive detection of multiple dyes after the reverse transcription polymerase chain reaction (RT-PCR). This experimental study reveals the multiplex detection capability of PCR-based SERS under existing PCR conditions without modifying primer and probe sequences. The combination of magnetic bead-based separation and paper SERS platform is efficient, economical, and can be used for the simultaneous detection of two or more pathogens.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>33606501</pmid><doi>10.1021/acs.analchem.0c05285</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-0330-7828</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2021-03, Vol.93 (8), p.3677-3685 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2491939891 |
| source | American Chemical Society Journals |
| subjects | Analytical chemistry Beads Biosensors Biotin Cellulose Cellulose fibers Chemistry Codification Dyes Fluorescent dyes Fluorescent indicators Light sources Multiplexing Plasmonics Polymerase chain reaction Primers (coatings) Probes Raman spectra Reverse transcription Separation Streptavidin Substrates Surface plasmon resonance |
| title | Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A01%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Paper-Based%20Multiplex%20Surface-Enhanced%20Raman%20Scattering%20Detection%20Using%20Polymerase%20Chain%20Reaction%20Probe%20Codification&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Kim,%20Eun%20Ju&rft.date=2021-03-02&rft.volume=93&rft.issue=8&rft.spage=3677&rft.epage=3685&rft.pages=3677-3685&rft.issn=0003-2700&rft.eissn=1520-6882&rft_id=info:doi/10.1021/acs.analchem.0c05285&rft_dat=%3Cproquest_cross%3E2491939891%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2498421324&rft_id=info:pmid/33606501&rfr_iscdi=true |