A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection
A sensitive and selective, aptamer and spectroscopic ellipsometry based sensor is reported here for the early diagnosis of breast cancer, which is a common type of cancer following lung cancer. It was aimed to develop a single-step and label-free assay for the sensitive and selective detection of VE...
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| Veröffentlicht in: | Talanta (Oxford) 2021-04, Vol.225, p.121982-121982, Article 121982 |
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| creator | Kariper, Ishak Afsin Üstündağ, Zafer Caglayan, Mustafa Oguzhan |
| description | A sensitive and selective, aptamer and spectroscopic ellipsometry based sensor is reported here for the early diagnosis of breast cancer, which is a common type of cancer following lung cancer. It was aimed to develop a single-step and label-free assay for the sensitive and selective detection of VEGF165. To this end, two different aptamers and spectroscopic ellipsometry were used. In the presented study, by determining the appropriate aptamer immobilization conditions, the spectroscopic ellipsometry technique was successfully applied for the detection of VEGF165 at the range of 1 pM–1000 pM in the buffer. Aptasensors have a detection limit of 5.81 pM and 4.29 pM, respectively.
[Display omitted]
•A spectroscopic ellipsometry based aptasensor is reported for the biomarker detection in cancer diagnosis.•Two different aptamers are implemented to detect the vascular endothelial growth factor as a model biomarker.•Detection limits of this label-free method are around 5 pM at the 1 pM to 1000 pM detection range. |
| doi_str_mv | 10.1016/j.talanta.2020.121982 |
| format | Article |
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[Display omitted]
•A spectroscopic ellipsometry based aptasensor is reported for the biomarker detection in cancer diagnosis.•Two different aptamers are implemented to detect the vascular endothelial growth factor as a model biomarker.•Detection limits of this label-free method are around 5 pM at the 1 pM to 1000 pM detection range.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2020.121982</identifier><identifier>PMID: 33592730</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aptamers ; Aptamers, Nucleotide ; Biosensing Techniques ; Biosensors ; Breast cancer ; Humans ; Limit of Detection ; Neoplasms ; Spectrophotometry ; Spectroscopic ellipsometry ; Vascular Endothelial Growth Factor A ; VEGF</subject><ispartof>Talanta (Oxford), 2021-04, Vol.225, p.121982-121982, Article 121982</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright © 2020 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-625bc6576d7997b715764dbc5d2d6ba044238adc1aae27243893a14fafd709f03</citedby><cites>FETCH-LOGICAL-c365t-625bc6576d7997b715764dbc5d2d6ba044238adc1aae27243893a14fafd709f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S003991402031273X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33592730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kariper, Ishak Afsin</creatorcontrib><creatorcontrib>Üstündağ, Zafer</creatorcontrib><creatorcontrib>Caglayan, Mustafa Oguzhan</creatorcontrib><title>A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>A sensitive and selective, aptamer and spectroscopic ellipsometry based sensor is reported here for the early diagnosis of breast cancer, which is a common type of cancer following lung cancer. It was aimed to develop a single-step and label-free assay for the sensitive and selective detection of VEGF165. To this end, two different aptamers and spectroscopic ellipsometry were used. In the presented study, by determining the appropriate aptamer immobilization conditions, the spectroscopic ellipsometry technique was successfully applied for the detection of VEGF165 at the range of 1 pM–1000 pM in the buffer. Aptasensors have a detection limit of 5.81 pM and 4.29 pM, respectively.
[Display omitted]
•A spectroscopic ellipsometry based aptasensor is reported for the biomarker detection in cancer diagnosis.•Two different aptamers are implemented to detect the vascular endothelial growth factor as a model biomarker.•Detection limits of this label-free method are around 5 pM at the 1 pM to 1000 pM detection range.</description><subject>Aptamers</subject><subject>Aptamers, Nucleotide</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Breast cancer</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Neoplasms</subject><subject>Spectrophotometry</subject><subject>Spectroscopic ellipsometry</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>VEGF</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P0zAQhi0EYsvCTwD5yCVlbCdxfULVavmQVuICZ2tiT6irNM7abtH-e1xauHKwbI-ed0bzMPZWwFqA6D_s1wUnnAuuJchak8Js5DO2EhutGtVp9ZytAJRpjGjhhr3KeQ8AUoF6yW6U6ozUClbsccszzTmUcCKeF3IlxWUXSzxQScFxmqaw5D-_Jz5gJs-3S8FzJiY-1lN2xE-Y3XHCxGn2sRamgBP_meKvsuMjulIxT6U2D3F-zV6MOGV6c71v2Y9P99_vvjQP3z5_vds-NE71XWl62Q2u73TvtTF60KI-Wz-4zkvfDwhtK9UGvROIJLVs1cYoFO2Io9dgRlC37P2l75Li45FysYeQXd0HZ4rHbGVroAcNva5od0FdijknGu2SwgHTkxVgz7bt3l5t27Nte7Fdc--uI47Dgfy_1F-9Ffh4AaguegqUbHaBZkc-pGrD-hj-M-I3eVqV6Q</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Kariper, Ishak Afsin</creator><creator>Üstündağ, Zafer</creator><creator>Caglayan, Mustafa Oguzhan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210401</creationdate><title>A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection</title><author>Kariper, Ishak Afsin ; Üstündağ, Zafer ; Caglayan, Mustafa Oguzhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-625bc6576d7997b715764dbc5d2d6ba044238adc1aae27243893a14fafd709f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aptamers</topic><topic>Aptamers, Nucleotide</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Breast cancer</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Neoplasms</topic><topic>Spectrophotometry</topic><topic>Spectroscopic ellipsometry</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>VEGF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kariper, Ishak Afsin</creatorcontrib><creatorcontrib>Üstündağ, Zafer</creatorcontrib><creatorcontrib>Caglayan, Mustafa Oguzhan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kariper, Ishak Afsin</au><au>Üstündağ, Zafer</au><au>Caglayan, Mustafa Oguzhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>225</volume><spage>121982</spage><epage>121982</epage><pages>121982-121982</pages><artnum>121982</artnum><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>A sensitive and selective, aptamer and spectroscopic ellipsometry based sensor is reported here for the early diagnosis of breast cancer, which is a common type of cancer following lung cancer. It was aimed to develop a single-step and label-free assay for the sensitive and selective detection of VEGF165. To this end, two different aptamers and spectroscopic ellipsometry were used. In the presented study, by determining the appropriate aptamer immobilization conditions, the spectroscopic ellipsometry technique was successfully applied for the detection of VEGF165 at the range of 1 pM–1000 pM in the buffer. Aptasensors have a detection limit of 5.81 pM and 4.29 pM, respectively.
[Display omitted]
•A spectroscopic ellipsometry based aptasensor is reported for the biomarker detection in cancer diagnosis.•Two different aptamers are implemented to detect the vascular endothelial growth factor as a model biomarker.•Detection limits of this label-free method are around 5 pM at the 1 pM to 1000 pM detection range.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33592730</pmid><doi>10.1016/j.talanta.2020.121982</doi><tpages>1</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 2021-04, Vol.225, p.121982-121982, Article 121982 |
| issn | 0039-9140 1873-3573 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Aptamers Aptamers, Nucleotide Biosensing Techniques Biosensors Breast cancer Humans Limit of Detection Neoplasms Spectrophotometry Spectroscopic ellipsometry Vascular Endothelial Growth Factor A VEGF |
| title | A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection |
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