Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer

Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer

Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism. A series of experiments were conducte...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2021-02, Vol.85 (3), p.568-578
Hauptverfasser: Huang, Shiping, Sun, Yueming
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 578
container_issue 3
container_start_page 568
container_title Bioscience, biotechnology, and biochemistry
container_volume 85
creator Huang, Shiping
Sun, Yueming
description Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism. A series of experiments were conducted to detect the effects of MNX1-AS1 and miR-744-5p on the biological function of CRC cells, including quantitative reverse transcription-polymerase chain reaction, CCK-8, transwell, wound healing assay, Western blot, and dual-luciferase report assay. MNX1-AS1 was elevated in CRC tissues and cell lines. Si-MNX1-AS1 inhibited cell viability, invasion, migration, and the protein expressions of N-cadherin and Vimentin but promoted the protein expression of E-cadherin. MiR-744-5p bound to MNX1-AS1. MiR-744-5p inhibitor had the opposite effect of si-MNX1-AS1. Cotransfection of miR-744-5p inhibitor and si-MNX1-AS1 recovered the effects mentioned above. In conclusion, MNX1-AS1/miR-744-5p axis plays a pivotal role in the viability, invasion, migration, and epithelial-mesenchymal transition of colorectal cancer cells.
doi_str_mv 10.1093/bbb/zbaa096
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2490124029</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2490124029</sourcerecordid><originalsourceid>FETCH-LOGICAL-c350t-bf7fcca133315b8d9451e27563fd30b52883809ee32d9b2f939c1181dc6628823</originalsourceid><addsrcrecordid>eNpNkUlLBDEQhYMo7ifvkqMgrUmntxwHcYMZBRfw1iTp6plId9Im6cP4X_yvZpxRhIIqqK_eo3gInVByQQlnl1LKy08pBOHFFtqnLCuTgmfl9r95Dx14_04I4TSnu2iPsZwTwqp99DW1Zo6NNco2Ok5PDxM8e3ijyeSZ4nY0KmhrPBaxsLL9AGFFgWnsHIwd_c9BsNjBfOxEAAyDDgvotOiSHjwYtVj2osPBCeP1SgzLJfZDdF0JzfRTUmZZkg9Ym2jQWQcqRF4Jo8AdoZ1WdB6ON_0Qvd5cv1zdJdPH2_uryTRRLCchkW3ZKiUoY4zmsmp4llNIy7xgbcOIzNOqYhXhACxtuExbzriitKKNKoq4S9khOlvrDs5-jOBD3WuvoOuEgfhknWac0DQjKY_o-RpVznrvoK0Hp3vhljUl9SqPOuZRb_KI9OlGeJQ9NH_sbwDsG7nQh64</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2490124029</pqid></control><display><type>article</type><title>Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer</title><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Huang, Shiping ; Sun, Yueming</creator><creatorcontrib>Huang, Shiping ; Sun, Yueming</creatorcontrib><description>Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism. A series of experiments were conducted to detect the effects of MNX1-AS1 and miR-744-5p on the biological function of CRC cells, including quantitative reverse transcription-polymerase chain reaction, CCK-8, transwell, wound healing assay, Western blot, and dual-luciferase report assay. MNX1-AS1 was elevated in CRC tissues and cell lines. Si-MNX1-AS1 inhibited cell viability, invasion, migration, and the protein expressions of N-cadherin and Vimentin but promoted the protein expression of E-cadherin. MiR-744-5p bound to MNX1-AS1. MiR-744-5p inhibitor had the opposite effect of si-MNX1-AS1. Cotransfection of miR-744-5p inhibitor and si-MNX1-AS1 recovered the effects mentioned above. In conclusion, MNX1-AS1/miR-744-5p axis plays a pivotal role in the viability, invasion, migration, and epithelial-mesenchymal transition of colorectal cancer cells.</description><identifier>ISSN: 1347-6947</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1093/bbb/zbaa096</identifier><identifier>PMID: 33590038</identifier><language>eng</language><publisher>England</publisher><ispartof>Bioscience, biotechnology, and biochemistry, 2021-02, Vol.85 (3), p.568-578</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-bf7fcca133315b8d9451e27563fd30b52883809ee32d9b2f939c1181dc6628823</citedby><cites>FETCH-LOGICAL-c350t-bf7fcca133315b8d9451e27563fd30b52883809ee32d9b2f939c1181dc6628823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33590038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Shiping</creatorcontrib><creatorcontrib>Sun, Yueming</creatorcontrib><title>Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism. A series of experiments were conducted to detect the effects of MNX1-AS1 and miR-744-5p on the biological function of CRC cells, including quantitative reverse transcription-polymerase chain reaction, CCK-8, transwell, wound healing assay, Western blot, and dual-luciferase report assay. MNX1-AS1 was elevated in CRC tissues and cell lines. Si-MNX1-AS1 inhibited cell viability, invasion, migration, and the protein expressions of N-cadherin and Vimentin but promoted the protein expression of E-cadherin. MiR-744-5p bound to MNX1-AS1. MiR-744-5p inhibitor had the opposite effect of si-MNX1-AS1. Cotransfection of miR-744-5p inhibitor and si-MNX1-AS1 recovered the effects mentioned above. In conclusion, MNX1-AS1/miR-744-5p axis plays a pivotal role in the viability, invasion, migration, and epithelial-mesenchymal transition of colorectal cancer cells.</description><issn>1347-6947</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpNkUlLBDEQhYMo7ifvkqMgrUmntxwHcYMZBRfw1iTp6plId9Im6cP4X_yvZpxRhIIqqK_eo3gInVByQQlnl1LKy08pBOHFFtqnLCuTgmfl9r95Dx14_04I4TSnu2iPsZwTwqp99DW1Zo6NNco2Ok5PDxM8e3ijyeSZ4nY0KmhrPBaxsLL9AGFFgWnsHIwd_c9BsNjBfOxEAAyDDgvotOiSHjwYtVj2osPBCeP1SgzLJfZDdF0JzfRTUmZZkg9Ym2jQWQcqRF4Jo8AdoZ1WdB6ON_0Qvd5cv1zdJdPH2_uryTRRLCchkW3ZKiUoY4zmsmp4llNIy7xgbcOIzNOqYhXhACxtuExbzriitKKNKoq4S9khOlvrDs5-jOBD3WuvoOuEgfhknWac0DQjKY_o-RpVznrvoK0Hp3vhljUl9SqPOuZRb_KI9OlGeJQ9NH_sbwDsG7nQh64</recordid><startdate>20210224</startdate><enddate>20210224</enddate><creator>Huang, Shiping</creator><creator>Sun, Yueming</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210224</creationdate><title>Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer</title><author>Huang, Shiping ; Sun, Yueming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-bf7fcca133315b8d9451e27563fd30b52883809ee32d9b2f939c1181dc6628823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Shiping</creatorcontrib><creatorcontrib>Sun, Yueming</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Shiping</au><au>Sun, Yueming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2021-02-24</date><risdate>2021</risdate><volume>85</volume><issue>3</issue><spage>568</spage><epage>578</epage><pages>568-578</pages><issn>1347-6947</issn><eissn>1347-6947</eissn><abstract>Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism. A series of experiments were conducted to detect the effects of MNX1-AS1 and miR-744-5p on the biological function of CRC cells, including quantitative reverse transcription-polymerase chain reaction, CCK-8, transwell, wound healing assay, Western blot, and dual-luciferase report assay. MNX1-AS1 was elevated in CRC tissues and cell lines. Si-MNX1-AS1 inhibited cell viability, invasion, migration, and the protein expressions of N-cadherin and Vimentin but promoted the protein expression of E-cadherin. MiR-744-5p bound to MNX1-AS1. MiR-744-5p inhibitor had the opposite effect of si-MNX1-AS1. Cotransfection of miR-744-5p inhibitor and si-MNX1-AS1 recovered the effects mentioned above. In conclusion, MNX1-AS1/miR-744-5p axis plays a pivotal role in the viability, invasion, migration, and epithelial-mesenchymal transition of colorectal cancer cells.</abstract><cop>England</cop><pmid>33590038</pmid><doi>10.1093/bbb/zbaa096</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1347-6947
ispartof Bioscience, biotechnology, and biochemistry, 2021-02, Vol.85 (3), p.568-578
issn 1347-6947
1347-6947
language eng
recordid cdi_proquest_miscellaneous_2490124029
source Oxford University Press Journals All Titles (1996-Current)
title Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T04%3A02%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Long%20noncoding%20RNA%20MNX1-AS1%20functions%20as%20a%20competing%20endogenous%20RNA%20to%20regulate%20epithelial-mesenchymal%20transition%20by%20sponging%20MiR-744-5p%20in%20colorectal%20cancer&rft.jtitle=Bioscience,%20biotechnology,%20and%20biochemistry&rft.au=Huang,%20Shiping&rft.date=2021-02-24&rft.volume=85&rft.issue=3&rft.spage=568&rft.epage=578&rft.pages=568-578&rft.issn=1347-6947&rft.eissn=1347-6947&rft_id=info:doi/10.1093/bbb/zbaa096&rft_dat=%3Cproquest_cross%3E2490124029%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2490124029&rft_id=info:pmid/33590038&rfr_iscdi=true