Comprehensive genome and transcriptome analysis of the dermatophyte Trichophyton schoenleinii reveals the candidate pathogenic genes

Background Trichophyton schoenleinii is an anthropophilic dermatophyte that causes tinea favosa. Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high‐quality reference ge...

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Veröffentlicht in:Mycoses 2021-06, Vol.64 (6), p.624-633
Hauptverfasser: Ge, Li‐Yu, Liu, Jia, Zheng, Hai‐Lin, Mei, Huan, Liang, Guan‐Zhao, Liu, Wei‐Da
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container_issue 6
container_start_page 624
container_title Mycoses
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creator Ge, Li‐Yu
Liu, Jia
Zheng, Hai‐Lin
Mei, Huan
Liang, Guan‐Zhao
Liu, Wei‐Da
description Background Trichophyton schoenleinii is an anthropophilic dermatophyte that causes tinea favosa. Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high‐quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile. Objectives The aim of the current study was to improve understanding of the underlying pathogenic mechanism of T. schoenleinii, and to define the candidate pathogenic genes of T. schoenleinii. Methods Comprehensive genomic analysis of T. schoenleinii was carried out by Illumina and PacBio sequencing platforms. Transcriptome profiles of T. schoenleinii cultured in vitro in two media containing either keratin or soy protein were determined using RNA sequencing (RNA‐seq) technology. Results Here, we present the first draft genome sequence of T. schoenleinii strain T2s, which consists of 11 scaffolds containing 7474 predicted genes. Transcriptome analysis showed that genes involved in keratin hydrolysis have higher expression in T. schoenleinii grown in keratin medium, including genes encoding proteases, cysteine dioxygenase and acetamidase. Other genes with higher expression include genes encoding the components of the pH‐responsive signal transduction pathways and transcription factors, many of which may play a role in pathogenicity. Conclusion In summary, this study provides new insights into the pathogenic mechanism of T. schoenleinii and highlights candidate genes for further development of novel targets in disease diagnosis and treatment of tinea favosa.
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Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high‐quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile. Objectives The aim of the current study was to improve understanding of the underlying pathogenic mechanism of T. schoenleinii, and to define the candidate pathogenic genes of T. schoenleinii. Methods Comprehensive genomic analysis of T. schoenleinii was carried out by Illumina and PacBio sequencing platforms. Transcriptome profiles of T. schoenleinii cultured in vitro in two media containing either keratin or soy protein were determined using RNA sequencing (RNA‐seq) technology. Results Here, we present the first draft genome sequence of T. schoenleinii strain T2s, which consists of 11 scaffolds containing 7474 predicted genes. Transcriptome analysis showed that genes involved in keratin hydrolysis have higher expression in T. schoenleinii grown in keratin medium, including genes encoding proteases, cysteine dioxygenase and acetamidase. Other genes with higher expression include genes encoding the components of the pH‐responsive signal transduction pathways and transcription factors, many of which may play a role in pathogenicity. Conclusion In summary, this study provides new insights into the pathogenic mechanism of T. schoenleinii and highlights candidate genes for further development of novel targets in disease diagnosis and treatment of tinea favosa.</description><identifier>ISSN: 0933-7407</identifier><identifier>EISSN: 1439-0507</identifier><identifier>DOI: 10.1111/myc.13257</identifier><identifier>PMID: 33586267</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Cysteine dioxygenase ; dermatophytes ; Gene expression ; genome ; Genomes ; Genomic analysis ; Keratin ; Medical importance ; Nucleotide sequence ; Pathogenicity ; Signal transduction ; Tinea ; tinea favosa ; Transcription factors ; transcriptome ; Transcriptomes ; Transcriptomics ; Trichophyton schoenleinii</subject><ispartof>Mycoses, 2021-06, Vol.64 (6), p.624-633</ispartof><rights>2021 Wiley‐VCH GmbH</rights><rights>2021 Wiley-VCH GmbH.</rights><rights>2021 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-b2392be43b709afa2b020b7779aadac51f552905f595bdbfe3c5ec51eff2d78f3</citedby><cites>FETCH-LOGICAL-c3537-b2392be43b709afa2b020b7779aadac51f552905f595bdbfe3c5ec51eff2d78f3</cites><orcidid>0000-0003-1936-3736 ; 0000-0003-4463-4585 ; 0000-0003-0247-1853</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fmyc.13257$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fmyc.13257$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33586267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Li‐Yu</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Zheng, Hai‐Lin</creatorcontrib><creatorcontrib>Mei, Huan</creatorcontrib><creatorcontrib>Liang, Guan‐Zhao</creatorcontrib><creatorcontrib>Liu, Wei‐Da</creatorcontrib><title>Comprehensive genome and transcriptome analysis of the dermatophyte Trichophyton schoenleinii reveals the candidate pathogenic genes</title><title>Mycoses</title><addtitle>Mycoses</addtitle><description>Background Trichophyton schoenleinii is an anthropophilic dermatophyte that causes tinea favosa. Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high‐quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile. Objectives The aim of the current study was to improve understanding of the underlying pathogenic mechanism of T. schoenleinii, and to define the candidate pathogenic genes of T. schoenleinii. Methods Comprehensive genomic analysis of T. schoenleinii was carried out by Illumina and PacBio sequencing platforms. Transcriptome profiles of T. schoenleinii cultured in vitro in two media containing either keratin or soy protein were determined using RNA sequencing (RNA‐seq) technology. Results Here, we present the first draft genome sequence of T. schoenleinii strain T2s, which consists of 11 scaffolds containing 7474 predicted genes. Transcriptome analysis showed that genes involved in keratin hydrolysis have higher expression in T. schoenleinii grown in keratin medium, including genes encoding proteases, cysteine dioxygenase and acetamidase. Other genes with higher expression include genes encoding the components of the pH‐responsive signal transduction pathways and transcription factors, many of which may play a role in pathogenicity. Conclusion In summary, this study provides new insights into the pathogenic mechanism of T. schoenleinii and highlights candidate genes for further development of novel targets in disease diagnosis and treatment of tinea favosa.</description><subject>Cysteine dioxygenase</subject><subject>dermatophytes</subject><subject>Gene expression</subject><subject>genome</subject><subject>Genomes</subject><subject>Genomic analysis</subject><subject>Keratin</subject><subject>Medical importance</subject><subject>Nucleotide sequence</subject><subject>Pathogenicity</subject><subject>Signal transduction</subject><subject>Tinea</subject><subject>tinea favosa</subject><subject>Transcription factors</subject><subject>transcriptome</subject><subject>Transcriptomes</subject><subject>Transcriptomics</subject><subject>Trichophyton schoenleinii</subject><issn>0933-7407</issn><issn>1439-0507</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kU1LxDAQhoMoun4c_AMS8KKHumnSbDZHWfwCxct68FTSdGIjbVOT7krv_nDjVj0IziXD8MwzkBeh45RcpLGmzaAvUka52EKTNGMyIZyIbTQhkrFEZETsof0QXglJhaSzXbTHGJ_P6ExM0MfCNZ2HCtpg14BfoHUNYNWWuPeqDdrbrh8nqh6CDdgZ3FeAS_CN6l1XDT3gpbe62vSuxSG20NZgW2uxhzWoOmxWdLTaUkW-U33l4imrvw5COEQ7JlJw9P0eoKfrq-XiNrl_vLlbXN4nmnEmkoIySQvIWCGIVEbRglBSCCGkUqXSPDWcU0m44ZIXZWGAaQ5xDMbQUswNO0Bno7fz7m0Foc8bGzTUtWrBrUJOs7nkUvIZjejpH_TVrXz8hEhxmpGMyHQeqfOR0t6F4MHknbeN8kOekvwrmjxGk2-iiezJt3FVNFD-kj9ZRGA6Au-2huF_U_7wvBiVn3Azm-A</recordid><startdate>202106</startdate><enddate>202106</enddate><creator>Ge, Li‐Yu</creator><creator>Liu, Jia</creator><creator>Zheng, Hai‐Lin</creator><creator>Mei, Huan</creator><creator>Liang, Guan‐Zhao</creator><creator>Liu, Wei‐Da</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1936-3736</orcidid><orcidid>https://orcid.org/0000-0003-4463-4585</orcidid><orcidid>https://orcid.org/0000-0003-0247-1853</orcidid></search><sort><creationdate>202106</creationdate><title>Comprehensive genome and transcriptome analysis of the dermatophyte Trichophyton schoenleinii reveals the candidate pathogenic genes</title><author>Ge, Li‐Yu ; Liu, Jia ; Zheng, Hai‐Lin ; Mei, Huan ; Liang, Guan‐Zhao ; Liu, Wei‐Da</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-b2392be43b709afa2b020b7779aadac51f552905f595bdbfe3c5ec51eff2d78f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cysteine dioxygenase</topic><topic>dermatophytes</topic><topic>Gene expression</topic><topic>genome</topic><topic>Genomes</topic><topic>Genomic analysis</topic><topic>Keratin</topic><topic>Medical importance</topic><topic>Nucleotide sequence</topic><topic>Pathogenicity</topic><topic>Signal transduction</topic><topic>Tinea</topic><topic>tinea favosa</topic><topic>Transcription factors</topic><topic>transcriptome</topic><topic>Transcriptomes</topic><topic>Transcriptomics</topic><topic>Trichophyton schoenleinii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Li‐Yu</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Zheng, Hai‐Lin</creatorcontrib><creatorcontrib>Mei, Huan</creatorcontrib><creatorcontrib>Liang, Guan‐Zhao</creatorcontrib><creatorcontrib>Liu, Wei‐Da</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Mycoses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Li‐Yu</au><au>Liu, Jia</au><au>Zheng, Hai‐Lin</au><au>Mei, Huan</au><au>Liang, Guan‐Zhao</au><au>Liu, Wei‐Da</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive genome and transcriptome analysis of the dermatophyte Trichophyton schoenleinii reveals the candidate pathogenic genes</atitle><jtitle>Mycoses</jtitle><addtitle>Mycoses</addtitle><date>2021-06</date><risdate>2021</risdate><volume>64</volume><issue>6</issue><spage>624</spage><epage>633</epage><pages>624-633</pages><issn>0933-7407</issn><eissn>1439-0507</eissn><abstract>Background Trichophyton schoenleinii is an anthropophilic dermatophyte that causes tinea favosa. Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high‐quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile. Objectives The aim of the current study was to improve understanding of the underlying pathogenic mechanism of T. schoenleinii, and to define the candidate pathogenic genes of T. schoenleinii. Methods Comprehensive genomic analysis of T. schoenleinii was carried out by Illumina and PacBio sequencing platforms. Transcriptome profiles of T. schoenleinii cultured in vitro in two media containing either keratin or soy protein were determined using RNA sequencing (RNA‐seq) technology. Results Here, we present the first draft genome sequence of T. schoenleinii strain T2s, which consists of 11 scaffolds containing 7474 predicted genes. Transcriptome analysis showed that genes involved in keratin hydrolysis have higher expression in T. schoenleinii grown in keratin medium, including genes encoding proteases, cysteine dioxygenase and acetamidase. Other genes with higher expression include genes encoding the components of the pH‐responsive signal transduction pathways and transcription factors, many of which may play a role in pathogenicity. Conclusion In summary, this study provides new insights into the pathogenic mechanism of T. schoenleinii and highlights candidate genes for further development of novel targets in disease diagnosis and treatment of tinea favosa.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33586267</pmid><doi>10.1111/myc.13257</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1936-3736</orcidid><orcidid>https://orcid.org/0000-0003-4463-4585</orcidid><orcidid>https://orcid.org/0000-0003-0247-1853</orcidid></addata></record>
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subjects Cysteine dioxygenase
dermatophytes
Gene expression
genome
Genomes
Genomic analysis
Keratin
Medical importance
Nucleotide sequence
Pathogenicity
Signal transduction
Tinea
tinea favosa
Transcription factors
transcriptome
Transcriptomes
Transcriptomics
Trichophyton schoenleinii
title Comprehensive genome and transcriptome analysis of the dermatophyte Trichophyton schoenleinii reveals the candidate pathogenic genes
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