Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water Samples

The present study demonstrates, development of ssDNA aptamers against whole cell of S. flexneri employing a whole bacterium-based Systemic Evolution of Ligands by Exponential Enrichment (SELEX). After ten rounds of SELEX, cell surface specific aptamer pool was cloned, sequenced and divided based on...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of fluorescence 2021-05, Vol.31 (3), p.685-693
Hauptverfasser:	Lavu, Padma Sudharani, Mondal, Bhairab, Ramlal, Shylaja
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - metabolism Bacteria Binding Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Flow cytometry Fluorescence Ligands Original Article Selectivity SELEX Aptamer Technique Shigella flexneri Target detection Water Microbiology Water sampling
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!