The effect of erythrocyte lysing reagents on enumeration of leukocyte subpopulations compared with a no‐lyse‐no‐wash protocol

Introduction Standard protocols in flow cytometry (FCM) require lysis of erythrocytes, which may induce an unwanted loss of leukocytes as bystander effect. Methods In the present study, we investigated the influence of 6 laboratory protocols using 4 different lysing reagents, FACS® Lysing Solution (...

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Veröffentlicht in:	International journal of laboratory hematology 2021-10, Vol.43 (5), p.939-947
Hauptverfasser:	Plank, Karin, Dorn, Carla, Krause, Stefan W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium chloride CD14 antigen CD16 antigen CD19 antigen CD3 antigen CD4 antigen CD45 antigen CD56 antigen CD8 antigen Enumeration Erythrocytes Flow cytometry Gating leukocyte preparation Leukocytes Light scattering loss of leukocytes lymphocyte subsets Lymphocytes Lysis Monocytes Peripheral blood Quantitation Reagents red blood cell lysis
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