Biochemical and Morphological Characterization of Heterotrophic Crypthecodinium cohnii and Chlorella vulgaris Cell Walls
Microalgae are attractive for the food and cosmetic industries because of their nutrient composition. However, the bioaccessibility and extractability of nutrients in microalgae are limited by the rigid and indigestible cell wall. The goal of this study is to explore the cell wall polysaccharides (C...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2021-02, Vol.69 (7), p.2226-2235 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Canelli, Greta Murciano Martínez, Patricia Austin, Sean Ambühl, Mark E Dionisi, Fabiola Bolten, Christoph J Carpine, Roberta Neutsch, Lukas Mathys, Alexander |
| description | Microalgae are attractive for the food and cosmetic industries because of their nutrient composition. However, the bioaccessibility and extractability of nutrients in microalgae are limited by the rigid and indigestible cell wall. The goal of this study is to explore the cell wall polysaccharides (CWPSs) composition and morphology in heterotrophic Crypthecodinium cohnii and Chlorella vulgaris biomasses during growth. Our results showed that glucose was the major component of CWPSs and exopolysaccharides in C. cohnii. C. vulgaris CWPSs have a similar sugar profile in exponential and stationary phases, essentially composed of rhamnose and galactose. C. vulgaris cell wall thickness increased from 82 nm in the exponential phase to 114 nm in the stationary phase and consisted of two main layers. C. cohnii’s cell wall was 133 nm thick and composed of several membranes surrounding thecal plates. Understanding of the microalgae cell wall helps developing a more efficient and targeted biorefinery approach. |
| doi_str_mv | 10.1021/acs.jafc.0c05032 |
| format | Article |
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However, the bioaccessibility and extractability of nutrients in microalgae are limited by the rigid and indigestible cell wall. The goal of this study is to explore the cell wall polysaccharides (CWPSs) composition and morphology in heterotrophic Crypthecodinium cohnii and Chlorella vulgaris biomasses during growth. Our results showed that glucose was the major component of CWPSs and exopolysaccharides in C. cohnii. C. vulgaris CWPSs have a similar sugar profile in exponential and stationary phases, essentially composed of rhamnose and galactose. C. vulgaris cell wall thickness increased from 82 nm in the exponential phase to 114 nm in the stationary phase and consisted of two main layers. C. cohnii’s cell wall was 133 nm thick and composed of several membranes surrounding thecal plates. 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Agric. Food Chem</addtitle><description>Microalgae are attractive for the food and cosmetic industries because of their nutrient composition. However, the bioaccessibility and extractability of nutrients in microalgae are limited by the rigid and indigestible cell wall. The goal of this study is to explore the cell wall polysaccharides (CWPSs) composition and morphology in heterotrophic Crypthecodinium cohnii and Chlorella vulgaris biomasses during growth. Our results showed that glucose was the major component of CWPSs and exopolysaccharides in C. cohnii. C. vulgaris CWPSs have a similar sugar profile in exponential and stationary phases, essentially composed of rhamnose and galactose. C. vulgaris cell wall thickness increased from 82 nm in the exponential phase to 114 nm in the stationary phase and consisted of two main layers. C. cohnii’s cell wall was 133 nm thick and composed of several membranes surrounding thecal plates. Understanding of the microalgae cell wall helps developing a more efficient and targeted biorefinery approach.</description><subject>Biofuels and Biobased Materials</subject><subject>Biomass</subject><subject>Cell Wall</subject><subject>Chlorella vulgaris</subject><subject>Dinoflagellida</subject><subject>Microalgae</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtPxCAURonROONj78qwdGFHKKWlS218JRo3GpcNpbeWCS0VWqP-epmH7lzdXHK-L9yD0AklC0pieiGVXyxloxZEEU5YvIPmlMck4pSKXTQngYkET-kMHXi_JIQInpF9NGMsTJanc_R5pa1qodNKGiz7Gj9aN7TW2Lf1S9FKJ9UITn_LUdse2wbfQdjt6OzQaoUL9zWMLShb615PHVa27bVeVxWtsQ6MkfhjMm_SaY-LsOJXaYw_QnuNNB6Ot_MQvdxcPxd30cPT7X1x-RBJxtIxShOWxDQFmoCSPAUFLMvySiRcQU3qWDGaiBSqRjVVXFcggMuK8lw0Sc6zVLBDdLbpHZx9n8CPZae9Wv2qBzv5Mk6E4EFRkgWUbFDlrPcOmnJwupPuq6SkXPkug-9y5bvc-g6R0237VHVQ_wV-BQfgfAOso3ZyfTj2_74fO_COqA</recordid><startdate>20210224</startdate><enddate>20210224</enddate><creator>Canelli, Greta</creator><creator>Murciano Martínez, Patricia</creator><creator>Austin, Sean</creator><creator>Ambühl, Mark E</creator><creator>Dionisi, Fabiola</creator><creator>Bolten, Christoph J</creator><creator>Carpine, Roberta</creator><creator>Neutsch, Lukas</creator><creator>Mathys, Alexander</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1770-0620</orcidid></search><sort><creationdate>20210224</creationdate><title>Biochemical and Morphological Characterization of Heterotrophic Crypthecodinium cohnii and Chlorella vulgaris Cell Walls</title><author>Canelli, Greta ; Murciano Martínez, Patricia ; Austin, Sean ; Ambühl, Mark E ; Dionisi, Fabiola ; Bolten, Christoph J ; Carpine, Roberta ; Neutsch, Lukas ; Mathys, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-6434216e14eca56ece3779b845ced0d2c31486ebfcfb2dbe8e5ab1598f4957683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biofuels and Biobased Materials</topic><topic>Biomass</topic><topic>Cell Wall</topic><topic>Chlorella vulgaris</topic><topic>Dinoflagellida</topic><topic>Microalgae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Canelli, Greta</creatorcontrib><creatorcontrib>Murciano Martínez, Patricia</creatorcontrib><creatorcontrib>Austin, Sean</creatorcontrib><creatorcontrib>Ambühl, Mark E</creatorcontrib><creatorcontrib>Dionisi, Fabiola</creatorcontrib><creatorcontrib>Bolten, Christoph J</creatorcontrib><creatorcontrib>Carpine, Roberta</creatorcontrib><creatorcontrib>Neutsch, Lukas</creatorcontrib><creatorcontrib>Mathys, Alexander</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Canelli, Greta</au><au>Murciano Martínez, Patricia</au><au>Austin, Sean</au><au>Ambühl, Mark E</au><au>Dionisi, Fabiola</au><au>Bolten, Christoph J</au><au>Carpine, Roberta</au><au>Neutsch, Lukas</au><au>Mathys, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical and Morphological Characterization of Heterotrophic Crypthecodinium cohnii and Chlorella vulgaris Cell Walls</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. 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| source | ACS Publications; MEDLINE |
| subjects | Biofuels and Biobased Materials Biomass Cell Wall Chlorella vulgaris Dinoflagellida Microalgae |
| title | Biochemical and Morphological Characterization of Heterotrophic Crypthecodinium cohnii and Chlorella vulgaris Cell Walls |
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