Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells
Sanhuang-Siwu-Tang (SST), composed of seven medicinal herbs, is a well-known herbal formula used for the treatment of gynecologic diseases. To expand the clinical use of SST, we explored the anti-inflammatory or anti-neuroinflammatory effects of SST water extract in lipopolysaccharide-stimulated RAW...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2021/04/01, Vol.44(4), pp.535-543 |
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| creator | Li, Jing Kim, Kwan-Woo Oh, Hyuncheol Kim, Youn-Chul |
| description | Sanhuang-Siwu-Tang (SST), composed of seven medicinal herbs, is a well-known herbal formula used for the treatment of gynecologic diseases. To expand the clinical use of SST, we explored the anti-inflammatory or anti-neuroinflammatory effects of SST water extract in lipopolysaccharide-stimulated RAW264.7 macrophages and BV2 microglial cells. According to HPLC analysis, the main components of SST were from Scutellariae Radix, Coptidis Rhizoma, and Paeoniae Radix. SST significantly inhibited pro-inflammatory mediators including lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW264.7 macrophages and BV2 microglial cells. Furthermore, these anti-inflammatory or anti-neuroinflammatory effects of SST were mediated by mitogen-activated protein kinase-related proteins (MAPK) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-related proteins. Overall, this study demonstrated that SST is a potential therapeutic formula for the prevention or treatment of inappropriate inflammation, neuroinflammation, or neurodegenerative diseases. |
| doi_str_mv | 10.1248/bpb.b20-00871 |
| format | Article |
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To expand the clinical use of SST, we explored the anti-inflammatory or anti-neuroinflammatory effects of SST water extract in lipopolysaccharide-stimulated RAW264.7 macrophages and BV2 microglial cells. According to HPLC analysis, the main components of SST were from Scutellariae Radix, Coptidis Rhizoma, and Paeoniae Radix. SST significantly inhibited pro-inflammatory mediators including lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW264.7 macrophages and BV2 microglial cells. Furthermore, these anti-inflammatory or anti-neuroinflammatory effects of SST were mediated by mitogen-activated protein kinase-related proteins (MAPK) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-related proteins. Overall, this study demonstrated that SST is a potential therapeutic formula for the prevention or treatment of inappropriate inflammation, neuroinflammation, or neurodegenerative diseases.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b20-00871</identifier><identifier>PMID: 33563883</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>BV2 microglial cell ; Cyclooxygenase-2 ; Herbal medicine ; High-performance liquid chromatography ; Inflammation ; Interleukin 6 ; Kinases ; Lipopolysaccharides ; Lymphocytes B ; Macrophages ; MAP kinase ; Medicinal plants ; Microglial cells ; Neurodegenerative diseases ; neuroinflammation ; NF-κB protein ; Nitric oxide ; Nitric-oxide synthase ; Prostaglandin E2 ; Protein kinase ; RAW264.7 macrophage ; Sanhuang-Siwu-Tang ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α</subject><ispartof>Biological and Pharmaceutical Bulletin, 2021/04/01, Vol.44(4), pp.535-543</ispartof><rights>2021 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c702t-571b6320f4e182130d87d3cc715807c14a795b352182082c016b37ed45b6d8083</citedby><cites>FETCH-LOGICAL-c702t-571b6320f4e182130d87d3cc715807c14a795b352182082c016b37ed45b6d8083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33563883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Kim, Kwan-Woo</creatorcontrib><creatorcontrib>Oh, Hyuncheol</creatorcontrib><creatorcontrib>Kim, Youn-Chul</creatorcontrib><creatorcontrib>College of Pharmacy</creatorcontrib><creatorcontrib>Wonkwang University</creatorcontrib><creatorcontrib>aInstitute of Pharmaceutical Research and Development</creatorcontrib><creatorcontrib>bDepartment of Pharmacy</creatorcontrib><creatorcontrib>Jiujiang University Affiliated Hospital</creatorcontrib><title>Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Sanhuang-Siwu-Tang (SST), composed of seven medicinal herbs, is a well-known herbal formula used for the treatment of gynecologic diseases. 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Overall, this study demonstrated that SST is a potential therapeutic formula for the prevention or treatment of inappropriate inflammation, neuroinflammation, or neurodegenerative diseases.</description><subject>BV2 microglial cell</subject><subject>Cyclooxygenase-2</subject><subject>Herbal medicine</subject><subject>High-performance liquid chromatography</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Kinases</subject><subject>Lipopolysaccharides</subject><subject>Lymphocytes B</subject><subject>Macrophages</subject><subject>MAP kinase</subject><subject>Medicinal plants</subject><subject>Microglial cells</subject><subject>Neurodegenerative diseases</subject><subject>neuroinflammation</subject><subject>NF-κB protein</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>Prostaglandin E2</subject><subject>Protein kinase</subject><subject>RAW264.7 macrophage</subject><subject>Sanhuang-Siwu-Tang</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc2P0zAQxSMEYsvCkSuyxIWLy_gjsXMs1bIgdYVEFzhajuO0rhw7xIlQL_ztuNulSFzskeenN2_8iuI1gSWhXL5vhmbZUMAAUpAnxYIwLnBJSfm0WEBNJK5IKa-KFykdAEAAZc-LK8bKiknJFsXvVZgcdqHzuu_1FMcjuuk6a6aEYoe2OuxnHXZ4637N-D5XyAW0cUMcoj8mbcxej661eDu5fvZ6si36uvpBK74U6E6bMQ57vbMJ6dCiD98punP5beed9mhtvU8vi2ed9sm-eryvi28fb-7Xn_Dmy-3n9WqDTXY84VKQpmIUOm6JpIRBK0XLjBF5NxCGcC3qsmF5bUlBUgOkapiwLS-bqpUg2XXx7qw7jPHnbNOkepdMdqCDjXNS-SclESAqmtG3_6GHOI8hu1O0BFHXVS1ZpvCZyvukNNpODaPr9XhUBNQpGJWDUTkY9RBM5t88qs5Nb9sL_TeJDNyegdx1RvsYvAv232yTROOij4oCJVmUc-AKSC5LVuaDMyaEIBKy0vqsdEhT_vzLKD1Oznj7YIxzxU_HxeCle0pU2cD-AG8mtT8</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Li, Jing</creator><creator>Kim, Kwan-Woo</creator><creator>Oh, Hyuncheol</creator><creator>Kim, Youn-Chul</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20210401</creationdate><title>Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells</title><author>Li, Jing ; Kim, Kwan-Woo ; Oh, Hyuncheol ; Kim, Youn-Chul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c702t-571b6320f4e182130d87d3cc715807c14a795b352182082c016b37ed45b6d8083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>BV2 microglial cell</topic><topic>Cyclooxygenase-2</topic><topic>Herbal medicine</topic><topic>High-performance liquid chromatography</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Kinases</topic><topic>Lipopolysaccharides</topic><topic>Lymphocytes B</topic><topic>Macrophages</topic><topic>MAP kinase</topic><topic>Medicinal plants</topic><topic>Microglial cells</topic><topic>Neurodegenerative diseases</topic><topic>neuroinflammation</topic><topic>NF-κB protein</topic><topic>Nitric oxide</topic><topic>Nitric-oxide synthase</topic><topic>Prostaglandin E2</topic><topic>Protein kinase</topic><topic>RAW264.7 macrophage</topic><topic>Sanhuang-Siwu-Tang</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Kim, Kwan-Woo</creatorcontrib><creatorcontrib>Oh, Hyuncheol</creatorcontrib><creatorcontrib>Kim, Youn-Chul</creatorcontrib><creatorcontrib>College of Pharmacy</creatorcontrib><creatorcontrib>Wonkwang University</creatorcontrib><creatorcontrib>aInstitute of Pharmaceutical Research and Development</creatorcontrib><creatorcontrib>bDepartment of Pharmacy</creatorcontrib><creatorcontrib>Jiujiang University Affiliated Hospital</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jing</au><au>Kim, Kwan-Woo</au><au>Oh, Hyuncheol</au><au>Kim, Youn-Chul</au><aucorp>College of Pharmacy</aucorp><aucorp>Wonkwang University</aucorp><aucorp>aInstitute of Pharmaceutical Research and Development</aucorp><aucorp>bDepartment of Pharmacy</aucorp><aucorp>Jiujiang University Affiliated Hospital</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>44</volume><issue>4</issue><spage>535</spage><epage>543</epage><pages>535-543</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Sanhuang-Siwu-Tang (SST), composed of seven medicinal herbs, is a well-known herbal formula used for the treatment of gynecologic diseases. To expand the clinical use of SST, we explored the anti-inflammatory or anti-neuroinflammatory effects of SST water extract in lipopolysaccharide-stimulated RAW264.7 macrophages and BV2 microglial cells. According to HPLC analysis, the main components of SST were from Scutellariae Radix, Coptidis Rhizoma, and Paeoniae Radix. SST significantly inhibited pro-inflammatory mediators including lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in LPS-stimulated RAW264.7 macrophages and BV2 microglial cells. Furthermore, these anti-inflammatory or anti-neuroinflammatory effects of SST were mediated by mitogen-activated protein kinase-related proteins (MAPK) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB)-related proteins. 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| subjects | BV2 microglial cell Cyclooxygenase-2 Herbal medicine High-performance liquid chromatography Inflammation Interleukin 6 Kinases Lipopolysaccharides Lymphocytes B Macrophages MAP kinase Medicinal plants Microglial cells Neurodegenerative diseases neuroinflammation NF-κB protein Nitric oxide Nitric-oxide synthase Prostaglandin E2 Protein kinase RAW264.7 macrophage Sanhuang-Siwu-Tang Tumor necrosis factor-TNF Tumor necrosis factor-α |
| title | Anti-inflammatory Effects of Sanhuang-Siwu-Tang in Lipopolysaccharide-Stimulated RAW264.7 Macrophages and BV2 Microglial Cells |
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