Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF‐MS
We aimed to develop a standardized methodology to determine the metabolic profile of organic extracts from Malvaviscus arboreus Cav. (Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these...
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| Veröffentlicht in: | Chemistry & biodiversity 2021-04, Vol.18 (4), p.e2000820-n/a |
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| creator | Rodríguez‐Morales, Sergio Ocampo‐Medina, Blanca Romero‐Ceronio, Nancy Alvarado‐Sánchez, Cuauhtémoc Vilchis‐Reyes, Miguel Ángel Roa de la Fuente, Luis Fernando Ortiz‐Andrade, Rolffy Hernández‐Abreu, Oswaldo |
| description | We aimed to develop a standardized methodology to determine the metabolic profile of organic extracts from Malvaviscus arboreus Cav. (Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these extracts by ex vivo rat thoracic aorta assay. Organic extracts of stems and leaves were prepared by a comprehensive maceration process. The vasorelaxant activity was determined by measuring the relaxant capability of the extract to decrease a contraction induced by noradrenaline (0.1 μM). The hexane extract induced a significant vasorelaxant effect in a concentration‐ and endothelium‐dependent manner. Secondary metabolites, such as polyunsaturated fatty acids, terpenes and one flavonoid, were annotated by liquid chromatography/quadrupole time‐of‐flight mass spectrometry (LC/QTOF‐MS) in positive ion mode. This exploratory study allowed us to identify bioactive secondary metabolites from Malvaviscus arboreus, as well as identify potentially‐new vasorelaxant molecules and scaffolds for drug discovery. |
| doi_str_mv | 10.1002/cbdv.202000820 |
| format | Article |
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(Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these extracts by ex vivo rat thoracic aorta assay. Organic extracts of stems and leaves were prepared by a comprehensive maceration process. The vasorelaxant activity was determined by measuring the relaxant capability of the extract to decrease a contraction induced by noradrenaline (0.1 μM). The hexane extract induced a significant vasorelaxant effect in a concentration‐ and endothelium‐dependent manner. Secondary metabolites, such as polyunsaturated fatty acids, terpenes and one flavonoid, were annotated by liquid chromatography/quadrupole time‐of‐flight mass spectrometry (LC/QTOF‐MS) in positive ion mode. This exploratory study allowed us to identify bioactive secondary metabolites from Malvaviscus arboreus, as well as identify potentially‐new vasorelaxant molecules and scaffolds for drug discovery.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202000820</identifier><identifier>PMID: 33560535</identifier><language>eng</language><publisher>Switzerland: Wiley Subscription Services, Inc</publisher><subject>Aorta ; bioactive ; Contraction ; Endothelium ; Fatty acids ; Flavonoids ; Hexanes ; Hypertension ; Ions ; LC/QTOF-MS ; Liquid chromatography ; Malvaviscus arboreus ; Mass spectrometry ; Mass spectroscopy ; Medicinal plants ; metabolic profiling ; Metabolism ; Metabolites ; Noradrenaline ; Norepinephrine ; pharmacognosy ; Polyunsaturated fatty acids ; Positive ions ; Quadrupoles ; Secondary metabolites ; Terpenes ; Thorax</subject><ispartof>Chemistry & biodiversity, 2021-04, Vol.18 (4), p.e2000820-n/a</ispartof><rights>2021 Wiley‐VHCA AG, Zurich, Switzerland</rights><rights>2021 Wiley-VHCA AG, Zurich, Switzerland.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3730-30b58842ecc78eed3e45b74070b242a8c7366892fb96bad576e46a482204760e3</citedby><cites>FETCH-LOGICAL-c3730-30b58842ecc78eed3e45b74070b242a8c7366892fb96bad576e46a482204760e3</cites><orcidid>0000-0002-2450-6722</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202000820$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202000820$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33560535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez‐Morales, Sergio</creatorcontrib><creatorcontrib>Ocampo‐Medina, Blanca</creatorcontrib><creatorcontrib>Romero‐Ceronio, Nancy</creatorcontrib><creatorcontrib>Alvarado‐Sánchez, Cuauhtémoc</creatorcontrib><creatorcontrib>Vilchis‐Reyes, Miguel Ángel</creatorcontrib><creatorcontrib>Roa de la Fuente, Luis Fernando</creatorcontrib><creatorcontrib>Ortiz‐Andrade, Rolffy</creatorcontrib><creatorcontrib>Hernández‐Abreu, Oswaldo</creatorcontrib><title>Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF‐MS</title><title>Chemistry & biodiversity</title><addtitle>Chem Biodivers</addtitle><description>We aimed to develop a standardized methodology to determine the metabolic profile of organic extracts from Malvaviscus arboreus Cav. (Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these extracts by ex vivo rat thoracic aorta assay. Organic extracts of stems and leaves were prepared by a comprehensive maceration process. The vasorelaxant activity was determined by measuring the relaxant capability of the extract to decrease a contraction induced by noradrenaline (0.1 μM). The hexane extract induced a significant vasorelaxant effect in a concentration‐ and endothelium‐dependent manner. Secondary metabolites, such as polyunsaturated fatty acids, terpenes and one flavonoid, were annotated by liquid chromatography/quadrupole time‐of‐flight mass spectrometry (LC/QTOF‐MS) in positive ion mode. This exploratory study allowed us to identify bioactive secondary metabolites from Malvaviscus arboreus, as well as identify potentially‐new vasorelaxant molecules and scaffolds for drug discovery.</description><subject>Aorta</subject><subject>bioactive</subject><subject>Contraction</subject><subject>Endothelium</subject><subject>Fatty acids</subject><subject>Flavonoids</subject><subject>Hexanes</subject><subject>Hypertension</subject><subject>Ions</subject><subject>LC/QTOF-MS</subject><subject>Liquid chromatography</subject><subject>Malvaviscus arboreus</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicinal plants</subject><subject>metabolic profiling</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Noradrenaline</subject><subject>Norepinephrine</subject><subject>pharmacognosy</subject><subject>Polyunsaturated fatty acids</subject><subject>Positive ions</subject><subject>Quadrupoles</subject><subject>Secondary metabolites</subject><subject>Terpenes</subject><subject>Thorax</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqF0MtOAyEUBmBiNF6qW5dmEjdu2p4BBuhSx1ZN2qjxsiVAGTNmWhRmarvzEXxGn0RMa03cyIYT8vHn5EfoMIVOCoC7Ro9nHQwYAASGDbSbshS3UyFgcz1zvIP2QniOPr6LbbRDSMYgI9kuehjZWmlXlSa58a4oq3L6lLgieVTBeVupuZrWSX9ee2XqpPBukoxUNVOzMpgmJMrrqOKgF8kw797eXw8-3z9Gd_toq1BVsAeru4UeBv37_LI9vL64yk-HbUM4gTYBnQlBsTWGC2vHxNJMcwocNKZYCcMJY6KHC91jWo0zzixligqMgXIGlrTQyTL3xbvXxoZaTuJitqrU1LomSEwF5zQeFunxH_rsGj-N20mcpRizlEEvqs5SGe9C8LaQL76cKL-QKcjvwuV34XJdePxwtIpt9MSO1_yn4Qh6S_BWVnbxT5zMz84ff8O_AEqwi0Q</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>Rodríguez‐Morales, Sergio</creator><creator>Ocampo‐Medina, Blanca</creator><creator>Romero‐Ceronio, Nancy</creator><creator>Alvarado‐Sánchez, Cuauhtémoc</creator><creator>Vilchis‐Reyes, Miguel Ángel</creator><creator>Roa de la Fuente, Luis Fernando</creator><creator>Ortiz‐Andrade, Rolffy</creator><creator>Hernández‐Abreu, Oswaldo</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2450-6722</orcidid></search><sort><creationdate>202104</creationdate><title>Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF‐MS</title><author>Rodríguez‐Morales, Sergio ; Ocampo‐Medina, Blanca ; Romero‐Ceronio, Nancy ; Alvarado‐Sánchez, Cuauhtémoc ; Vilchis‐Reyes, Miguel Ángel ; Roa de la Fuente, Luis Fernando ; Ortiz‐Andrade, Rolffy ; Hernández‐Abreu, Oswaldo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3730-30b58842ecc78eed3e45b74070b242a8c7366892fb96bad576e46a482204760e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aorta</topic><topic>bioactive</topic><topic>Contraction</topic><topic>Endothelium</topic><topic>Fatty acids</topic><topic>Flavonoids</topic><topic>Hexanes</topic><topic>Hypertension</topic><topic>Ions</topic><topic>LC/QTOF-MS</topic><topic>Liquid chromatography</topic><topic>Malvaviscus arboreus</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicinal plants</topic><topic>metabolic profiling</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Noradrenaline</topic><topic>Norepinephrine</topic><topic>pharmacognosy</topic><topic>Polyunsaturated fatty acids</topic><topic>Positive ions</topic><topic>Quadrupoles</topic><topic>Secondary metabolites</topic><topic>Terpenes</topic><topic>Thorax</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez‐Morales, Sergio</creatorcontrib><creatorcontrib>Ocampo‐Medina, Blanca</creatorcontrib><creatorcontrib>Romero‐Ceronio, Nancy</creatorcontrib><creatorcontrib>Alvarado‐Sánchez, Cuauhtémoc</creatorcontrib><creatorcontrib>Vilchis‐Reyes, Miguel Ángel</creatorcontrib><creatorcontrib>Roa de la Fuente, Luis Fernando</creatorcontrib><creatorcontrib>Ortiz‐Andrade, Rolffy</creatorcontrib><creatorcontrib>Hernández‐Abreu, Oswaldo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry & biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez‐Morales, Sergio</au><au>Ocampo‐Medina, Blanca</au><au>Romero‐Ceronio, Nancy</au><au>Alvarado‐Sánchez, Cuauhtémoc</au><au>Vilchis‐Reyes, Miguel Ángel</au><au>Roa de la Fuente, Luis Fernando</au><au>Ortiz‐Andrade, Rolffy</au><au>Hernández‐Abreu, Oswaldo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF‐MS</atitle><jtitle>Chemistry & biodiversity</jtitle><addtitle>Chem Biodivers</addtitle><date>2021-04</date><risdate>2021</risdate><volume>18</volume><issue>4</issue><spage>e2000820</spage><epage>n/a</epage><pages>e2000820-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>We aimed to develop a standardized methodology to determine the metabolic profile of organic extracts from Malvaviscus arboreus Cav. (Malvaceae), a Mexican plant used in traditional medicine for the treatment of hypertension and other illnesses. Also, we determined the vasorelaxant activity of these extracts by ex vivo rat thoracic aorta assay. Organic extracts of stems and leaves were prepared by a comprehensive maceration process. The vasorelaxant activity was determined by measuring the relaxant capability of the extract to decrease a contraction induced by noradrenaline (0.1 μM). The hexane extract induced a significant vasorelaxant effect in a concentration‐ and endothelium‐dependent manner. Secondary metabolites, such as polyunsaturated fatty acids, terpenes and one flavonoid, were annotated by liquid chromatography/quadrupole time‐of‐flight mass spectrometry (LC/QTOF‐MS) in positive ion mode. This exploratory study allowed us to identify bioactive secondary metabolites from Malvaviscus arboreus, as well as identify potentially‐new vasorelaxant molecules and scaffolds for drug discovery.</abstract><cop>Switzerland</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33560535</pmid><doi>10.1002/cbdv.202000820</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2450-6722</orcidid></addata></record> |
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| subjects | Aorta bioactive Contraction Endothelium Fatty acids Flavonoids Hexanes Hypertension Ions LC/QTOF-MS Liquid chromatography Malvaviscus arboreus Mass spectrometry Mass spectroscopy Medicinal plants metabolic profiling Metabolism Metabolites Noradrenaline Norepinephrine pharmacognosy Polyunsaturated fatty acids Positive ions Quadrupoles Secondary metabolites Terpenes Thorax |
| title | Metabolic Profiling of Vasorelaxant Extract from Malvaviscus arboreus by LC/QTOF‐MS |
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