Untargeted metabolomic profiling of serum in dogs with hypothyroidism
Hypothyroidism is one of the most commonly diagnosed endocrine disease in dogs. The clinical signs are caused by a deficiency of the active thyroid hormones triiodothyronine (T3) and thyroxine (T4) and have a negative impact on dog's quality of life. We hypothesized that serum metabolic profile...
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| Veröffentlicht in: | Research in veterinary science 2021-05, Vol.136, p.6-10 |
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| creator | Muñoz-Prieto, Alberto González-Arostegui, Luis Guillermo Rubić, Ivana Cerón, José Joaquín Tvarijonaviciute, Asta Horvatić, Anita Mrljak, Vladimir |
| description | Hypothyroidism is one of the most commonly diagnosed endocrine disease in dogs. The clinical signs are caused by a deficiency of the active thyroid hormones triiodothyronine (T3) and thyroxine (T4) and have a negative impact on dog's quality of life. We hypothesized that serum metabolic profile varies between healthy dogs and dogs with hypothyroidism. Twenty serum samples from dogs with hypothyroidism and 20 from healthy dogs were used for untargeted metabolomics analysis performed by LC/MS analysis. Fifteen metabolites showed significant changes between hypothyroid and healthy dogs, being the pentose phosphate pathway (PPP), aminoacyl-tRNA biosynthesis and pyrimidine metabolism the principal pathways altered in hypothyroidism. Specifically, metabolites such as D-gluconic acid and L-Isoleucine may potentially act as biomarkers of disease.
•Metabolomics (Met) improves the understanding of physiopathology in diseases.•Met is an opportunity for biomarkers identification in dogs with hypothyroidism.•Hypothyroidism have a significant impact in the serum metabolic profile of dogs.•d-gluconic acid and l-Isoleucine were upregulated in canine hypothyroidism. |
| doi_str_mv | 10.1016/j.rvsc.2021.01.023 |
| format | Article |
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•Metabolomics (Met) improves the understanding of physiopathology in diseases.•Met is an opportunity for biomarkers identification in dogs with hypothyroidism.•Hypothyroidism have a significant impact in the serum metabolic profile of dogs.•d-gluconic acid and l-Isoleucine were upregulated in canine hypothyroidism.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2021.01.023</identifier><identifier>PMID: 33550147</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Biomarkers ; Biosynthesis ; Chromatography ; Discriminant analysis ; Disease ; Dogs ; Endocrine ; Endocrine disorders ; Females ; Gluconic acid ; Hormones ; Hypothyroidism ; Isoleucine ; Metabolism ; Metabolites ; Metabolomics ; Pentose ; Pentose phosphate pathway ; Principal components analysis ; Quality of life ; Thyroid ; Thyroid gland ; Thyroid hormones ; Thyroxine ; Triiodothyronine ; tRNA ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2021-05, Vol.136, p.6-10</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited May 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-931aa273a212fe07d8120956337522d437ed1ffd065fc63e0273aa23730b9f103</citedby><cites>FETCH-LOGICAL-c384t-931aa273a212fe07d8120956337522d437ed1ffd065fc63e0273aa23730b9f103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2021.01.023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33550147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muñoz-Prieto, Alberto</creatorcontrib><creatorcontrib>González-Arostegui, Luis Guillermo</creatorcontrib><creatorcontrib>Rubić, Ivana</creatorcontrib><creatorcontrib>Cerón, José Joaquín</creatorcontrib><creatorcontrib>Tvarijonaviciute, Asta</creatorcontrib><creatorcontrib>Horvatić, Anita</creatorcontrib><creatorcontrib>Mrljak, Vladimir</creatorcontrib><title>Untargeted metabolomic profiling of serum in dogs with hypothyroidism</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Hypothyroidism is one of the most commonly diagnosed endocrine disease in dogs. The clinical signs are caused by a deficiency of the active thyroid hormones triiodothyronine (T3) and thyroxine (T4) and have a negative impact on dog's quality of life. We hypothesized that serum metabolic profile varies between healthy dogs and dogs with hypothyroidism. Twenty serum samples from dogs with hypothyroidism and 20 from healthy dogs were used for untargeted metabolomics analysis performed by LC/MS analysis. Fifteen metabolites showed significant changes between hypothyroid and healthy dogs, being the pentose phosphate pathway (PPP), aminoacyl-tRNA biosynthesis and pyrimidine metabolism the principal pathways altered in hypothyroidism. Specifically, metabolites such as D-gluconic acid and L-Isoleucine may potentially act as biomarkers of disease.
•Metabolomics (Met) improves the understanding of physiopathology in diseases.•Met is an opportunity for biomarkers identification in dogs with hypothyroidism.•Hypothyroidism have a significant impact in the serum metabolic profile of dogs.•d-gluconic acid and l-Isoleucine were upregulated in canine hypothyroidism.</description><subject>Biomarkers</subject><subject>Biosynthesis</subject><subject>Chromatography</subject><subject>Discriminant analysis</subject><subject>Disease</subject><subject>Dogs</subject><subject>Endocrine</subject><subject>Endocrine disorders</subject><subject>Females</subject><subject>Gluconic acid</subject><subject>Hormones</subject><subject>Hypothyroidism</subject><subject>Isoleucine</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Pentose</subject><subject>Pentose phosphate pathway</subject><subject>Principal components analysis</subject><subject>Quality of life</subject><subject>Thyroid</subject><subject>Thyroid gland</subject><subject>Thyroid hormones</subject><subject>Thyroxine</subject><subject>Triiodothyronine</subject><subject>tRNA</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1r3DAQhkVpaLZJ_0APxdBLL96MZizLC72UkH5AoJfkLLTWaFeLbW0lO2H_fW027aGHwgtzed6X4RHivYS1BFnfHNbpKbdrBJRrmIP0SqykIiyxruVrsQKgqlTYNJfibc4HAKik1G_EJZFSICu9EnePw2jTjkd2Rc-j3cYu9qEtjin60IVhV0RfZE5TX4ShcHGXi-cw7ov96RjH_SnF4ELur8WFt13mdy_3Sjx-vXu4_V7e__z24_bLfdlSU43lhqS1qMmiRM-gXSMRNqom0grRVaTZSe8d1Mq3NTEsrEXSBNuNl0BX4tN5d37v18R5NH3ILXedHThO2WDV6IpqBDWjH_9BD3FKw_ydQYVakSJJM4Vnqk0x58TeHFPobToZCWaRbA5mkWwWyQbm4FL68DI9bXt2fyt_rM7A5zPAs4unwMnkNvDQsguJ29G4GP63_xvSrIwJ</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Muñoz-Prieto, Alberto</creator><creator>González-Arostegui, Luis Guillermo</creator><creator>Rubić, Ivana</creator><creator>Cerón, José Joaquín</creator><creator>Tvarijonaviciute, Asta</creator><creator>Horvatić, Anita</creator><creator>Mrljak, Vladimir</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202105</creationdate><title>Untargeted metabolomic profiling of serum in dogs with hypothyroidism</title><author>Muñoz-Prieto, Alberto ; González-Arostegui, Luis Guillermo ; Rubić, Ivana ; Cerón, José Joaquín ; Tvarijonaviciute, Asta ; Horvatić, Anita ; Mrljak, Vladimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-931aa273a212fe07d8120956337522d437ed1ffd065fc63e0273aa23730b9f103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomarkers</topic><topic>Biosynthesis</topic><topic>Chromatography</topic><topic>Discriminant analysis</topic><topic>Disease</topic><topic>Dogs</topic><topic>Endocrine</topic><topic>Endocrine disorders</topic><topic>Females</topic><topic>Gluconic acid</topic><topic>Hormones</topic><topic>Hypothyroidism</topic><topic>Isoleucine</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Pentose</topic><topic>Pentose phosphate pathway</topic><topic>Principal components analysis</topic><topic>Quality of life</topic><topic>Thyroid</topic><topic>Thyroid gland</topic><topic>Thyroid hormones</topic><topic>Thyroxine</topic><topic>Triiodothyronine</topic><topic>tRNA</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muñoz-Prieto, Alberto</creatorcontrib><creatorcontrib>González-Arostegui, Luis Guillermo</creatorcontrib><creatorcontrib>Rubić, Ivana</creatorcontrib><creatorcontrib>Cerón, José Joaquín</creatorcontrib><creatorcontrib>Tvarijonaviciute, Asta</creatorcontrib><creatorcontrib>Horvatić, Anita</creatorcontrib><creatorcontrib>Mrljak, Vladimir</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muñoz-Prieto, Alberto</au><au>González-Arostegui, Luis Guillermo</au><au>Rubić, Ivana</au><au>Cerón, José Joaquín</au><au>Tvarijonaviciute, Asta</au><au>Horvatić, Anita</au><au>Mrljak, Vladimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Untargeted metabolomic profiling of serum in dogs with hypothyroidism</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2021-05</date><risdate>2021</risdate><volume>136</volume><spage>6</spage><epage>10</epage><pages>6-10</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>Hypothyroidism is one of the most commonly diagnosed endocrine disease in dogs. The clinical signs are caused by a deficiency of the active thyroid hormones triiodothyronine (T3) and thyroxine (T4) and have a negative impact on dog's quality of life. We hypothesized that serum metabolic profile varies between healthy dogs and dogs with hypothyroidism. Twenty serum samples from dogs with hypothyroidism and 20 from healthy dogs were used for untargeted metabolomics analysis performed by LC/MS analysis. Fifteen metabolites showed significant changes between hypothyroid and healthy dogs, being the pentose phosphate pathway (PPP), aminoacyl-tRNA biosynthesis and pyrimidine metabolism the principal pathways altered in hypothyroidism. Specifically, metabolites such as D-gluconic acid and L-Isoleucine may potentially act as biomarkers of disease.
•Metabolomics (Met) improves the understanding of physiopathology in diseases.•Met is an opportunity for biomarkers identification in dogs with hypothyroidism.•Hypothyroidism have a significant impact in the serum metabolic profile of dogs.•d-gluconic acid and l-Isoleucine were upregulated in canine hypothyroidism.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>33550147</pmid><doi>10.1016/j.rvsc.2021.01.023</doi><tpages>5</tpages></addata></record> |
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| subjects | Biomarkers Biosynthesis Chromatography Discriminant analysis Disease Dogs Endocrine Endocrine disorders Females Gluconic acid Hormones Hypothyroidism Isoleucine Metabolism Metabolites Metabolomics Pentose Pentose phosphate pathway Principal components analysis Quality of life Thyroid Thyroid gland Thyroid hormones Thyroxine Triiodothyronine tRNA Veterinary medicine |
| title | Untargeted metabolomic profiling of serum in dogs with hypothyroidism |
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