Enzyme engineering and its industrial applications

Recently, there has been an increase in the demand for enzymes with modified activity, specificity, and stability. Enzyme engineering is an important tool to meet the demand for enzymes adjusted to different industrial processes. Knowledge of the structure and function of enzymes guides the choice o...

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Veröffentlicht in:Biotechnology and applied biochemistry 2022-04, Vol.69 (2), p.389-409
Hauptverfasser: Victorino da Silva Amatto, Isabela, Gonsales da Rosa‐Garzon, Nathalia, Antônio de Oliveira Simões, Flávio, Santiago, Fernanda, Pereira da Silva Leite, Nathália, Raspante Martins, Júlia, Cabral, Hamilton
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container_end_page 409
container_issue 2
container_start_page 389
container_title Biotechnology and applied biochemistry
container_volume 69
creator Victorino da Silva Amatto, Isabela
Gonsales da Rosa‐Garzon, Nathalia
Antônio de Oliveira Simões, Flávio
Santiago, Fernanda
Pereira da Silva Leite, Nathália
Raspante Martins, Júlia
Cabral, Hamilton
description Recently, there has been an increase in the demand for enzymes with modified activity, specificity, and stability. Enzyme engineering is an important tool to meet the demand for enzymes adjusted to different industrial processes. Knowledge of the structure and function of enzymes guides the choice of the best strategy for engineering enzymes. Each enzyme engineering strategy, such as rational design, directed evolution, and semi‐rational design, has specific applications, as well as limitations, which must be considered when choosing a suitable strategy. Engineered enzymes can be optimized for different industrial applications by choosing the appropriate strategy. This review features engineered enzymes that have been applied in food, animal feed, pharmaceuticals, medical applications, bioremediation, biofuels, and detergents. Graphical Integration of various techniques for obtaining modified enzymes.
doi_str_mv 10.1002/bab.2117
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Animal feed
Animals
Biocatalysis
Biodegradation, Environmental
Biofuels
Bioremediation
Biotechnology
Detergents
Directed evolution
Engineering
Enzymes
Enzymes - chemistry
Feeds
Industrial applications
Industry
Protein Engineering
rational design
semi‐rational design
Structure-function relationships
title Enzyme engineering and its industrial applications
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